Standard name | |
---|---|
Human Ortholog | |
Description | C-3 sterol dehydrogenase; catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; human homolog NSDHL implicated in CK syndrome, and can complement yeast null mutant; molecular target of natural product and antifungal compound FR171456 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 1 | 12 | 4 | 6 | 8 | 15 | 13 | 7 | 17 | 7 | 12 | 3 | 4 | 2 | 0 | – | – | 3 | 3 | 2 | 6 | 4 | 6 |
Bud | 1 | 0 | 3 | 2 | 1 | 3 | 4 | 4 | 0 | 4 | 1 | 3 | 1 | 1 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 1 | 0 | 2 | 0 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 13 | 8 | 26 | 29 | 25 | 15 | 3 | 9 | 10 | 13 | 10 | 15 | 30 | 7 | – | – | 0 | 0 | 3 | 4 | 10 | 10 |
Cytoplasm | 15 | 27 | 42 | 29 | 41 | 43 | 39 | 36 | 21 | 22 | 17 | 20 | 24 | 34 | 13 | 5 | – | – | 3 | 7 | 6 | 3 | 5 | 9 |
Endoplasmic Reticulum | 146 | 78 | 42 | 75 | 100 | 100 | 115 | 98 | 90 | 78 | 39 | 72 | 68 | 74 | 107 | 0 | – | – | 179 | 89 | 124 | 111 | 186 | 235 |
Endosome | 1 | 0 | 3 | 0 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | 3 | 0 | 0 | 0 | 0 | 1 |
Golgi | 1 | 0 | 0 | 0 | 4 | 3 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | – | – | 1 | 0 | 2 | 0 | 0 | 1 |
Mitochondria | 19 | 1 | 5 | 15 | 13 | 12 | 33 | 19 | 41 | 37 | 20 | 23 | 1 | 2 | 0 | 0 | – | – | 7 | 2 | 6 | 6 | 7 | 6 |
Nucleus | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 1 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 4 | 5 | 2 | 0 | 1 | 3 | 8 | 1 | 2 | 7 | 2 | 8 | 1 | 0 | 1 | 0 | – | – | 1 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 10 | 1 | 39 | 19 | 66 | 37 | 52 | 63 | 9 | 45 | 12 | 39 | 4 | 8 | 4 | 7 | – | – | 5 | 2 | 2 | 1 | 13 | 11 |
Unique Cell Count | 183 | 101 | 129 | 128 | 204 | 186 | 223 | 206 | 144 | 171 | 91 | 156 | 97 | 119 | 131 | 18 | 208 | 108 | 161 | 138 | 236 | 289 | ||
Labelled Cell Count | 204 | 116 | 163 | 152 | 260 | 240 | 295 | 254 | 173 | 221 | 109 | 191 | 115 | 140 | 158 | 22 | 208 | 108 | 161 | 138 | 236 | 289 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.6 | 11.2 | 9.1 | 9.1 | 10.4 | 9.6 | 9.4 | 10.4 | 8.9 | 9.0 | 9.1 | 9.8 | 9.8 | 10.6 | 10.5 | 9.9 | – | – | 9.7 | 9.5 | 10.1 |
Std Deviation (1e-4) | 1.2 | 2.0 | 1.7 | 1.7 | 1.9 | 1.8 | 1.8 | 2.1 | 1.7 | 1.8 | 1.8 | 2.0 | 2.0 | 1.6 | 1.5 | 1.7 | – | – | 1.7 | 1.7 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.0 | 0.19 | 0.08 | 0.05 | 0.2 | -0.02 | -0.01 | -0.0 | 0.11 | 0.1 | 0.23 | 0.21 | 0.13 | – | – | 0.09 | 0.07 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.0 | -2.5 | -1.8 | -0.9 | -1.0 | -1.4 | 0.2 | -0.4 | -0.5 | -1.9 | -1.9 | -2.8 | 0 | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Cell Periphery | -1.1 | 0.7 | 1.4 | 0.3 | -0.9 | -2.8 | -1.6 | 0.2 | -0.5 | 0.1 | 0.6 | 2.8 | 0 | – | – |
Cytoplasm | -1.8 | -2.6 | -1.9 | -3.2 | -3.2 | -3.5 | -4.1 | -2.3 | -4.0 | -1.3 | -0.7 | -4.5 | -0.4 | – | – |
Endoplasmic Reticulum | 4.2 | 3.0 | 3.7 | 3.5 | 2.7 | 4.9 | 2.3 | 1.6 | 2.3 | 5.6 | 4.7 | 8.0 | -2.9 | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Mitochondria | 2.3 | 1.0 | 1.0 | 3.2 | 1.8 | 5.4 | 4.4 | 4.2 | 3.1 | 0 | 0 | 0 | 0 | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Vacuole | -3.0 | 0.4 | -2.1 | -1.4 | 0.1 | -5.2 | -0.7 | -3.0 | -1.0 | -4.9 | -4.7 | -5.9 | 0.7 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.6897 | 6.4083 | 6.2842 | 5.8058 | 5.632 | 6.5429 | 4.8306 | 5.5751 | 5.1035 | 4.7624 | 4.7537 | 5.2775 | 4.3307 | 4.7867 | 4.5679 | 2.85 | 2.9026 | 3.8696 |
Actin | 0.0193 | 0.0013 | 0.0025 | 0.0372 | 0.0069 | 0.0011 | 0.0081 | 0.0003 | 0.003 | 0.005 | 0.0037 | 0.0008 | 0.0265 | 0.0113 | 0.0021 | 0.0014 | 0.0006 | 0.042 |
Bud | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0 | 0.0009 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0005 |
Bud Neck | 0.0016 | 0.0001 | 0.0053 | 0.0013 | 0.0004 | 0.0002 | 0.0007 | 0.0003 | 0.0033 | 0.001 | 0.0042 | 0.0005 | 0.0017 | 0.0018 | 0.0004 | 0.0004 | 0.0004 | 0.001 |
Bud Periphery | 0.0005 | 0.0003 | 0.0003 | 0.0006 | 0.0005 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0023 | 0.0007 | 0.0002 | 0.0015 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0013 |
Bud Site | 0.008 | 0.0002 | 0.0021 | 0.0026 | 0.0005 | 0 | 0.0015 | 0.0113 | 0.006 | 0.0064 | 0.0005 | 0.0001 | 0.0117 | 0.0057 | 0.0006 | 0.0002 | 0.0002 | 0.0005 |
Cell Periphery | 0.0015 | 0.0009 | 0.002 | 0.0014 | 0.0012 | 0.0003 | 0.001 | 0.0021 | 0.0012 | 0.0044 | 0.0048 | 0.0008 | 0.0008 | 0.0044 | 0.0013 | 0.0018 | 0.003 | 0.0004 |
Cytoplasm | 0.0611 | 0.0515 | 0.0468 | 0.0377 | 0.0296 | 0.0278 | 0.0609 | 0.0911 | 0.0804 | 0.0332 | 0.1001 | 0.0762 | 0.0652 | 0.092 | 0.0777 | 0.0689 | 0.0513 | 0.0516 |
Cytoplasmic Foci | 0.0142 | 0.0165 | 0.0087 | 0.0079 | 0.0039 | 0.0015 | 0.0094 | 0.0011 | 0.0097 | 0.0125 | 0.0211 | 0.0022 | 0.0246 | 0.0109 | 0.0169 | 0.0019 | 0.0021 | 0.0069 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0003 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0.0004 |
Endoplasmic Reticulum | 0.7745 | 0.8409 | 0.8905 | 0.7928 | 0.8257 | 0.9298 | 0.7896 | 0.8596 | 0.8183 | 0.8878 | 0.7054 | 0.8773 | 0.6393 | 0.8131 | 0.8474 | 0.8422 | 0.8505 | 0.7481 |
Endosome | 0.0352 | 0.0262 | 0.0074 | 0.0293 | 0.0412 | 0.0106 | 0.0337 | 0.0064 | 0.0248 | 0.0059 | 0.0536 | 0.0054 | 0.0506 | 0.0067 | 0.0204 | 0.0206 | 0.0492 | 0.0118 |
Golgi | 0.0108 | 0.0054 | 0.0019 | 0.0338 | 0.0256 | 0.0022 | 0.0085 | 0.0002 | 0.0067 | 0.0023 | 0.0163 | 0.0012 | 0.0204 | 0.0045 | 0.009 | 0.0017 | 0.0014 | 0.0114 |
Lipid Particles | 0.0087 | 0.0095 | 0.0042 | 0.0083 | 0.0121 | 0.0023 | 0.0152 | 0.0029 | 0.0108 | 0.003 | 0.0568 | 0.0083 | 0.0258 | 0.0044 | 0.0058 | 0.0104 | 0.0087 | 0.0243 |
Mitochondria | 0.0061 | 0.007 | 0.0022 | 0.0029 | 0.0123 | 0.0024 | 0.011 | 0.0002 | 0.0109 | 0.0005 | 0.0062 | 0.0033 | 0.0295 | 0.0014 | 0.0026 | 0.0008 | 0.001 | 0.0384 |
None | 0.0074 | 0.0071 | 0.0044 | 0.0053 | 0.0066 | 0.0021 | 0.0138 | 0.0018 | 0.0051 | 0.0016 | 0.0009 | 0.0083 | 0.0025 | 0.0085 | 0.0007 | 0.0005 | 0.0012 | 0.0009 |
Nuclear Periphery | 0.0326 | 0.0217 | 0.0089 | 0.0204 | 0.0193 | 0.0107 | 0.0219 | 0.0137 | 0.0088 | 0.0144 | 0.0092 | 0.0101 | 0.055 | 0.0157 | 0.0079 | 0.0191 | 0.019 | 0.0361 |
Nucleolus | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0.001 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0006 | 0.0009 | 0 | 0.0002 | 0 | 0.0001 |
Nucleus | 0.0038 | 0.0018 | 0.0009 | 0.0038 | 0.0042 | 0.0021 | 0.0026 | 0.0018 | 0.0017 | 0.0028 | 0.0019 | 0.0014 | 0.0093 | 0.0037 | 0.0007 | 0.0018 | 0.0016 | 0.002 |
Peroxisomes | 0.0032 | 0.0029 | 0.0067 | 0.0016 | 0.0004 | 0.0012 | 0.0076 | 0 | 0.0022 | 0.0052 | 0.0077 | 0.0005 | 0.0199 | 0.0012 | 0.0027 | 0.0001 | 0 | 0.0158 |
Punctate Nuclear | 0.0024 | 0.0008 | 0.0007 | 0.0015 | 0.0012 | 0.0022 | 0.0038 | 0.0006 | 0.0006 | 0.0064 | 0.0013 | 0.0005 | 0.003 | 0.0098 | 0.0006 | 0.0004 | 0.0003 | 0.0028 |
Vacuole | 0.0065 | 0.0043 | 0.0037 | 0.0081 | 0.0057 | 0.0024 | 0.0067 | 0.0057 | 0.0049 | 0.0037 | 0.0043 | 0.0026 | 0.0066 | 0.003 | 0.0025 | 0.025 | 0.0068 | 0.0022 |
Vacuole Periphery | 0.0018 | 0.0014 | 0.0004 | 0.0025 | 0.0019 | 0.0006 | 0.0021 | 0.0004 | 0.0011 | 0.0006 | 0.0011 | 0.0003 | 0.0042 | 0.0003 | 0.0003 | 0.0023 | 0.0025 | 0.0016 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 71.984 | 73.2737 | 57.1605 | 68.8725 | 57.9057 | 84.0224 | 75.2742 | 76.9287 | 79.6431 | 83.9712 |
Translational Efficiency | 1.7213 | 1.2182 | 1.5164 | 1.346 | 1.3913 | 1.5134 | 1.4954 | 1.2382 | 1.2702 | 1.2265 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1820 | 661 | 323 | 1694 | 1831 | 1670 | 226 | 1363 | 3651 | 2331 | 549 | 3057 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1045.66 | 1108.58 | 1512.07 | 1345.97 | 1108.63 | 1057.61 | 1580.27 | 1373.71 | 1077.24 | 1072.06 | 1540.15 | 1358.34 |
Standard Deviation | 159.52 | 176.87 | 230.39 | 259.20 | 197.53 | 157.10 | 217.45 | 252.09 | 182.33 | 164.56 | 227.64 | 256.42 |
Intensity Change Log 2 | — | 0.084299 | 0.532111 | 0.364232 | — | -0.067970 | 0.511393 | 0.309300 | — | 0.007947 | 0.521486 | 0.336224 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000708 | 0.002326 | 0.002071 | 0.003700 | 0.000479 | 0.001501 | 0.001122 | 0.003314 | 0.000593 | 0.001735 | 0.001680 | 0.003528 |
Bud Neck | 0.000662 | 0.002076 | 0.006543 | 0.003696 | 0.000194 | 0.001180 | 0.005605 | 0.004093 | 0.000427 | 0.001434 | 0.006157 | 0.003873 |
Bud Site | 0.005865 | 0.014036 | 0.016914 | 0.024497 | 0.002426 | 0.005474 | 0.011503 | 0.027711 | 0.004140 | 0.007902 | 0.014687 | 0.025930 |
Cell Periphery | 0.004166 | 0.003170 | 0.004548 | 0.016018 | 0.003752 | 0.002373 | 0.003133 | 0.019330 | 0.003958 | 0.002599 | 0.003966 | 0.017495 |
Cytoplasm | 0.041250 | 0.035711 | 0.022078 | 0.048770 | 0.016256 | 0.049747 | 0.020674 | 0.052903 | 0.028715 | 0.045766 | 0.021500 | 0.050613 |
Cytoplasmic Foci | 0.006272 | 0.015374 | 0.004821 | 0.006204 | 0.002366 | 0.010576 | 0.005982 | 0.006139 | 0.004313 | 0.011937 | 0.005299 | 0.006175 |
Eisosomes | 0.000417 | 0.000813 | 0.000189 | 0.000314 | 0.000175 | 0.000476 | 0.000064 | 0.000438 | 0.000295 | 0.000572 | 0.000138 | 0.000369 |
Endoplasmic Reticulum | 0.911621* | 0.871983* | 0.747474* | 0.804196* | 0.957562* | 0.884740* | 0.764539* | 0.808880* | 0.934661* | 0.881123* | 0.754499* | 0.806284* |
Endosome | 0.003209 | 0.010568 | 0.022093 | 0.011076 | 0.003017 | 0.005145 | 0.019879 | 0.011116 | 0.003113 | 0.006683 | 0.021182 | 0.011094 |
Golgi | 0.003977 | 0.007508 | 0.001479 | 0.008461 | 0.001524 | 0.008640 | 0.001204 | 0.005644 | 0.002747 | 0.008319 | 0.001366 | 0.007205 |
Lipid Particles | 0.005991 | 0.010665 | 0.023849 | 0.025660 | 0.003686 | 0.009114 | 0.016734 | 0.026928 | 0.004835 | 0.009554 | 0.020920 | 0.026225 |
Mitochondria | 0.003135 | 0.002978 | 0.000534 | 0.001577 | 0.000939 | 0.003479 | 0.000153 | 0.001069 | 0.002034 | 0.003336 | 0.000377 | 0.001350 |
Mitotic Spindle | 0.000385 | 0.000488 | 0.002662 | 0.002901 | 0.000101 | 0.000865 | 0.002273 | 0.001547 | 0.000242 | 0.000758 | 0.002501 | 0.002297 |
None | 0.002446 | 0.001055 | 0.000059 | 0.001442 | 0.001670 | 0.003113 | 0.000039 | 0.001341 | 0.002057 | 0.002529 | 0.000051 | 0.001397 |
Nuclear Periphery | 0.000275 | 0.000842 | 0.019087 | 0.000476 | 0.000405 | 0.000333 | 0.010250 | 0.000445 | 0.000340 | 0.000477 | 0.015449 | 0.000462 |
Nuclear Periphery Foci | 0.002946 | 0.006403 | 0.076665 | 0.009097 | 0.002267 | 0.003774 | 0.083354 | 0.006766 | 0.002605 | 0.004519 | 0.079419 | 0.008058 |
Nucleolus | 0.000030 | 0.000083 | 0.000025 | 0.000152 | 0.000010 | 0.000039 | 0.000017 | 0.000031 | 0.000020 | 0.000051 | 0.000022 | 0.000098 |
Nucleus | 0.001518 | 0.002780 | 0.002316 | 0.002583 | 0.000406 | 0.002662 | 0.001786 | 0.002576 | 0.000960 | 0.002695 | 0.002098 | 0.002580 |
Peroxisomes | 0.002437 | 0.001593 | 0.000099 | 0.000714 | 0.001031 | 0.001946 | 0.000133 | 0.000914 | 0.001731 | 0.001846 | 0.000113 | 0.000803 |
Vacuole | 0.001746 | 0.007250 | 0.045266 | 0.025960 | 0.001156 | 0.003477 | 0.050374 | 0.017038 | 0.001450 | 0.004547 | 0.047369 | 0.021982 |
Vacuole Periphery | 0.000944 | 0.002299 | 0.001227 | 0.002507 | 0.000580 | 0.001347 | 0.001184 | 0.001776 | 0.000762 | 0.001617 | 0.001209 | 0.002181 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.70 | -3.09 | -7.09 | -2.30 | -2.57 | -2.38 | -2.51 | -6.07 | -3.15 | -3.91 | -3.25 | -3.91 | -9.40 | -4.35 | -4.19 |
Bud Neck | -2.22 | -4.32 | -5.97 | -2.29 | 2.11 | -2.93 | -3.74 | -6.71 | -4.44 | 1.19 | -3.24 | -5.75 | -9.04 | -5.35 | 2.40 |
Bud Site | -3.04 | -2.87 | -8.31 | -3.64 | -1.66 | -3.03 | -2.49 | -9.35 | -7.99 | -3.14 | -3.41 | -3.90 | -12.64 | -9.76 | -3.22 |
Cell Periphery | 1.20 | -0.51 | -7.77 | -8.51 | -6.44 | 2.26 | 0.52 | -9.85 | -11.18 | -9.05 | 2.81 | -0.19 | -12.34 | -14.14 | -10.64 |
Cytoplasm | 1.31 | 3.26 | -3.15 | -3.78 | -5.21 | -11.11 | -1.44 | -9.58 | -1.13 | -4.68 | -6.70 | 1.15 | -9.12 | -2.59 | -7.01 |
Cytoplasmic Foci | -4.19 | 0.61 | -0.20 | 4.11 | -0.78 | -6.54 | -2.32 | -4.02 | 2.93 | 0.16 | -6.84 | -1.52 | -2.92 | 4.74 | -0.41 |
Eisosomes | -0.75 | 1.77 | 0.83 | 0.96 | -1.83 | -3.45 | 1.87 | -3.08 | 0.37 | -5.40 | -1.67 | 2.15 | -1.07 | 1.26 | -4.65 |
Endoplasmic Reticulum | 3.76 | 9.71 | 13.76 | 6.63 | -3.01 | 11.82 | 9.83 | 18.02 | 8.40 | -2.49 | 9.81 | 14.07 | 22.54 | 11.62 | -3.94 |
Endosome | -6.06 | -8.86 | -10.20 | -0.52 | 5.05 | -3.14 | -7.99 | -9.06 | -5.72 | 3.99 | -5.98 | -11.86 | -13.71 | -5.83 | 6.42 |
Golgi | -2.38 | 4.51 | -3.26 | -0.45 | -5.38 | -6.37 | 1.08 | -4.06 | 2.04 | -4.38 | -6.05 | 4.34 | -5.09 | 0.99 | -6.88 |
Lipid Particles | -2.24 | -3.96 | -9.75 | -5.67 | -0.18 | -5.11 | -4.49 | -9.69 | -7.14 | -2.06 | -4.86 | -5.58 | -13.87 | -9.91 | -1.15 |
Mitochondria | 0.18 | 4.70 | 2.54 | 1.56 | -1.83 | -4.64 | 4.73 | -0.96 | 4.30 | -5.93 | -2.66 | 5.34 | 1.94 | 3.86 | -2.98 |
Mitotic Spindle | -0.34 | -2.98 | -3.13 | -3.02 | 0.14 | -1.82 | -2.40 | -2.32 | -1.05 | 1.00 | -1.59 | -3.87 | -3.93 | -2.70 | 0.80 |
None | 2.16 | 4.55 | 1.57 | -0.82 | -4.12 | -1.68 | 4.27 | 0.57 | 2.00 | -3.04 | -0.73 | 6.19 | 1.54 | 1.79 | -5.07 |
Nuclear Periphery | -3.82 | -5.93 | -3.88 | 2.20 | 5.85 | 0.79 | -5.00 | -0.63 | -2.52 | 4.98 | -2.05 | -7.40 | -2.68 | -0.14 | 7.33 |
Nuclear Periphery Foci | -3.37 | -9.62 | -5.52 | -2.42 | 8.69 | -2.01 | -7.63 | -5.78 | -3.14 | 7.19 | -3.02 | -12.24 | -7.72 | -4.52 | 11.27 |
Nucleolus | -1.13 | -0.46 | -1.11 | -0.58 | -1.04 | -2.97 | -1.66 | -4.21 | 0.63 | -1.37 | -2.07 | -1.11 | -1.29 | -0.75 | -1.10 |
Nucleus | -0.94 | -2.16 | -4.37 | -0.33 | -0.50 | -6.23 | -2.36 | -5.53 | -0.87 | 0.04 | -3.69 | -3.37 | -7.29 | -0.97 | -0.27 |
Peroxisomes | 1.23 | 4.40 | 3.20 | 1.96 | -4.26 | -2.92 | 4.51 | 0.53 | 3.62 | -5.02 | -0.33 | 5.71 | 3.17 | 4.43 | -6.54 |
Vacuole | -4.78 | -7.42 | -12.65 | -9.21 | 2.89 | -3.96 | -6.63 | -11.73 | -9.60 | 4.39 | -5.76 | -9.97 | -16.69 | -13.73 | 5.13 |
Vacuole Periphery | -2.61 | -0.87 | -2.89 | -0.29 | -2.20 | -2.36 | -0.89 | -3.41 | -0.99 | -0.87 | -3.02 | -1.36 | -4.25 | -1.42 | -2.20 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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