Standard name
Description Ubiquitin-protein ligase; forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.72 0.79 0.67 0.65 0.81 0.72 0.69 0.54 0.62 0.47 0.58 0.52 0.46 0.45 0.66 0.59 0.54 0.4 0.35 0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0 0.14 0.23 0.46 0.37 0.53 0.29 0 0 0 0 0 0 0 0 0.14
Nucleus 0 0.08 0.1 0.07 0.06 0.11 0.12 0.1 0.09 0.1 0.09 0.18 0.1 0.15 0.18 0.21 0.17 0.25 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.26 0.21 0.27 0.28 0.19 0.15 0.11 0.1 0.1 0.1 0.13 0.42 0.52 0.49 0.07 0.14 0.18 0.35 0.62 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 0 0 0
Bud 0 2 0 2 1 1 1 6 5 7 4 0 0 0 0 2 4 0 0 0
Bud Neck 0 0 4 1 0 0 5 0 2 1 3 0 0 0 0 3 2 0 0 0
Bud Site 0 0 0 0 0 1 1 1 1 2 3 0 0 0
Cell Periphery 0 1 1 0 2 0 0 2 2 1 3 0 0 0 0 0 0 0 0 0
Cytoplasm 55 250 151 132 221 246 332 240 349 205 248 131 73 57 77 234 159 2 2 2
Endoplasmic Reticulum 0 4 1 0 0 1 1 0 0 1 9 10 3 4 1 0 1 0 0 0
Endosome 0 1 0 6 1 2 0 2 4 2 2 15 7 5 3 3 1 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 6 5 0 9 4 46 113 204 209 231 124 3 4 1 0 2 10 0 0 0
Nucleus 0 24 23 15 16 37 57 46 49 44 38 45 16 19 20 82 49 1 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 2 3 0 0 1 0 1 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 0 1 3 1 4 2 0 0 1 4 6 0 0 0
Vac/Vac Membrane 20 66 61 57 52 51 51 46 54 45 54 106 83 62 8 53 54 2 4 1
Unique Cell Count 76 318 225 204 272 340 482 443 565 440 426 254 159 126 118 395 297 7 7 5
Labelled Cell Count 83 353 241 222 297 387 561 549 682 544 493 313 187 148 118 395 297 7 7 5


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 5.0 5.8 5.3 5.6 4.4 4.4 4.0 3.9 3.9 4.1 6.9 7.3 6.9
Std Deviation (1e-4) 0.6 0.6 1.0 1.1 1.3 0.8 0.8 1.1 0.8 0.9 0.5 1.2 1.3 1.1
Intensity Change (Log2) -0.13 -0.04 -0.39 -0.39 -0.52 -0.57 -0.58 -0.49 0.25 0.34 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 3.6 1.3 0.5 -3.2 -1.4 -5.0 -2.2 -3.4 -4.2 -4.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 2.5 0 0
Endosome 0 0 0 0 0 0 0 0 3.7 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0
Nucleus -1.0 -1.8 0.2 0.6 0.1 -0.7 -0.1 -0.5 2.3 -0.1 1.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.2 -2.1 -3.5 -5.6 -5.6 -6.3 -5.6 -4.6 3.4 5.0 4.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cul3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available