Standard name
Human Ortholog
Description Mucin family member involved in various signaling pathways; functions as osmosensor in the Sho1p-mediated HOG pathway; functions in Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; processed into secreted and cell-associated forms by aspartyl protease Yps1p; potential Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.05 0.09 0.07 0.12 0.12 0.15 – 0.21 0.28 0.31 0.47 0.49 0.7 0.16 0.2 0.23 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 – 0 0 0 0.08 0.13 0 0 0 0 0 0 0 0.08 0.06 0.1
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.11 0.11 0.05 0 0.08 0.08 0.06 – 0.08 0.08 0.07 0 0 0 0 0.07 0 0 0 0 0 0 0
Nucleus 0.23 0.21 0.32 0.39 0.17 0.32 0.31 0.27 – 0.23 0.2 0.22 0.08 0.08 0.05 0.45 0.44 0.36 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.71 0.72 0.59 0.71 0.79 0.67 0.66 0.72 – 0.7 0.68 0.66 0.44 0.41 0.24 0.44 0.37 0.43 0.86 0.89 0.89 0.79 0.82 0.75
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 2 – 1 0 0 3 3 3 0 0 0 0 0 1 0 0 3
Bud 0 1 0 0 0 0 1 4 – 2 2 3 1 1 1 0 0 0 6 10 4 6 3 5
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 4 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 17 14 17 28 24 57 74 130 – 146 147 203 75 75 133 51 80 45 1 1 2 2 3 2
Endoplasmic Reticulum 1 1 3 1 3 5 2 3 – 2 4 2 1 2 4 8 16 12 1 2 0 2 2 2
Endosome 16 14 11 3 16 1 5 1 – 0 0 0 13 20 7 10 12 8 9 10 7 19 19 34
Golgi 2 5 1 0 3 0 0 1 – 0 0 0 0 0 0 8 0 3 3 1 2 1 3 3
Mitochondria 14 46 36 16 6 39 47 49 – 57 41 45 1 1 0 14 29 5 8 4 4 6 9 6
Nucleus 83 86 100 116 56 157 189 231 – 162 103 143 12 12 10 140 180 70 8 9 5 7 6 2
Nuclear Periphery 6 5 9 1 0 0 1 3 – 1 2 3 0 0 1 5 4 3 6 2 5 4 9 12
Nucleolus 12 13 6 0 2 1 4 5 – 2 2 0 0 0 0 0 5 3 4 3 0 0 0 1
Peroxisomes 1 1 0 0 2 0 1 1 – 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 1 0 0 1 1 1 – 2 1 0 5 3 1 5 10 0 2 1 0 1 0 0
Vac/Vac Membrane 255 288 188 214 263 326 403 610 – 492 356 429 70 63 45 137 151 82 352 398 321 206 272 248
Unique Cell Count 360 401 316 301 333 489 607 848 698 521 648 158 154 189 314 408 192 410 448 360 262 334 331
Labelled Cell Count 408 476 372 379 375 587 728 1041 868 658 829 181 180 209 378 487 231 410 448 360 262 334 331


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.3 10.8 9.6 9.9 13.5 12.5 12.5 12.4 – 11.1 10.9 10.0 7.2 7.2 6.7 8.3 8.2 10.0 10.4 11.4 11.9
Std Deviation (1e-4) 5.7 4.1 3.7 3.8 4.8 5.9 6.4 6.1 – 5.6 5.6 4.5 2.4 2.4 1.9 3.3 4.2 4.3 4.7 4.8 4.7
Intensity Change (Log2) – – – 0.04 0.49 0.38 0.38 0.37 – 0.22 0.18 0.06 -0.4 -0.4 -0.53 -0.21 -0.23 0.06 0.12 0.24 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 1.0 3.0 3.3 4.5 – 6.2 8.1 9.0 10.9 11.1 15.5 4.4 5.6 6.0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 1.5 2.5 3.4
Endosome -2.1 0.8 0 -2.9 0 – 0 0 0 2.2 3.9 0.1 -0.2 -0.4 0.4
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria -2.7 -5.0 -1.6 -1.8 -3.3 – -1.6 -1.7 -2.3 -4.1 -4.1 -4.8 -3.2 -2.0 -3.5
Nucleus 1.8 -4.4 0.1 -0.2 -1.5 – -2.8 -3.9 -3.2 -5.8 -5.7 -6.9 3.3 3.4 1.1
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 -1.1 -1.9 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 3.0 5.4 2.1 2.1 4.1 – 3.4 2.6 2.0 -3.1 -3.8 -7.8 -4.0 -6.0 -3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6146 9.1505 7.6919 7.4673 6.8805 8.4311 6.8343 12.6542 10.4373 10.383 11.0128 11.1833 6.9879 11.3743 9.3325 9.8968 9.2704 10.0943
Actin 0.0062 0 0.0017 0.0001 0.0288 0.0002 0.0004 0 0 0 0 0 0.0001 0.0005 0.0016 0.0002 0.0015 0
Bud 0.0004 0.0012 0.0004 0.0002 0.0025 0.0004 0.0002 0.0004 0.0003 0.0002 0.0003 0.0002 0.0002 0.0005 0.0008 0.0008 0.0002 0.0003
Bud Neck 0.001 0.0011 0.0012 0.0005 0.0015 0.0012 0.0007 0.0008 0.0007 0.0008 0.0007 0.0006 0.0004 0.0012 0.0036 0.0016 0.0005 0.0012
Bud Periphery 0.0009 0.0028 0.001 0.0006 0.0033 0.0014 0.0008 0.0012 0.001 0.0005 0.001 0.0011 0.0008 0.0016 0.0028 0.002 0.0005 0.0014
Bud Site 0.0041 0.0015 0.0014 0.0004 0.0186 0.001 0.0005 0.0009 0.0006 0.0004 0.0005 0.0003 0.0004 0.0014 0.0048 0.0016 0.0005 0.0004
Cell Periphery 0.0064 0.0109 0.0067 0.0057 0.0031 0.0092 0.0073 0.013 0.0105 0.0065 0.0088 0.0107 0.005 0.0246 0.0124 0.0076 0.0105 0.0141
Cytoplasm 0.009 0.0014 0.0036 0.0042 0.0013 0.0011 0.0007 0.001 0.0007 0.0006 0.0004 0.0004 0.0005 0.0018 0.0011 0.0006 0.0005 0.0004
Cytoplasmic Foci 0.017 0.0023 0.0072 0.0025 0.0145 0.0015 0.0018 0.0029 0.0014 0.0016 0.0007 0.0005 0.0122 0.0082 0.0204 0.0071 0.0363 0.001
Eisosomes 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0037 0.0017 0.0027 0.0013 0.0014 0.0015 0.0017 0.0023 0.0023 0.0007 0.0006 0.0016 0.0007 0.006 0.0016 0.0007 0.0005 0.0012
Endosome 0.0961 0.0332 0.106 0.0499 0.0782 0.0459 0.0768 0.037 0.0747 0.067 0.0493 0.0405 0.1454 0.0643 0.1072 0.0878 0.1234 0.0399
Golgi 0.009 0.0029 0.0091 0.0036 0.0191 0.0018 0.0035 0.0015 0.0034 0.0016 0.002 0.0017 0.0075 0.0073 0.0266 0.0133 0.0829 0.003
Lipid Particles 0.0287 0.0058 0.0211 0.0386 0.008 0.0087 0.0136 0.0049 0.0119 0.0062 0.0042 0.0069 0.0658 0.0653 0.0646 0.1059 0.0708 0.0546
Mitochondria 0.0096 0.0033 0.0073 0.0273 0.0322 0.0025 0.018 0.0035 0.0217 0.002 0.0126 0.0131 0.0619 0.0236 0.0821 0.0193 0.0308 0.0049
None 0.0012 0.0002 0.0008 0.0002 0.0003 0.0001 0.0003 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001
Nuclear Periphery 0.0118 0.0031 0.0043 0.0031 0.0084 0.0048 0.0043 0.0028 0.0026 0.0009 0.001 0.0037 0.0017 0.0055 0.0022 0.001 0.0006 0.0024
Nucleolus 0.0055 0.0064 0.0089 0.0036 0.0043 0.0045 0.0044 0.0052 0.004 0.0067 0.002 0.0033 0.0048 0.0075 0.0104 0.0036 0.003 0.011
Nucleus 0.0081 0.007 0.0066 0.0055 0.0139 0.0046 0.0026 0.0036 0.002 0.0019 0.0014 0.003 0.0022 0.0022 0.0038 0.0018 0.001 0.0037
Peroxisomes 0.0111 0.0004 0.0008 0.0026 0.0174 0.0005 0.0009 0.0029 0.0005 0.0004 0.0003 0.0003 0.0014 0.0037 0.0097 0.0022 0.0127 0.0006
Punctate Nuclear 0.0095 0.0001 0.0043 0.0007 0.0008 0.0016 0.0002 0.0001 0 0 0 0 0.0001 0.0001 0.0002 0.0002 0.0004 0.0001
Vacuole 0.5304 0.6981 0.6024 0.6653 0.5792 0.694 0.5583 0.6781 0.6197 0.7065 0.6706 0.6409 0.3707 0.5591 0.468 0.5301 0.4605 0.5839
Vacuole Periphery 0.2303 0.2167 0.2023 0.1841 0.1631 0.2134 0.3032 0.2379 0.2419 0.1953 0.2434 0.2712 0.3182 0.2153 0.1758 0.2127 0.1627 0.276

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 90.6137 62.8906 27.6272 46.4053 113.6536 65.8132 73.6992 56.9093 41.8934 150.404
Translational Efficiency 0.9323 0.9441 1.0187 0.3547 0.8715 1.1312 0.686 0.6435 0.7014 0.6468

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mucin family member involved in various signaling pathways; functions as osmosensor in the Sho1p-mediated HOG pathway; functions in Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; processed into secreted and cell-associated forms by aspartyl protease Yps1p; potential Cdc28p substrate
Localization
Cell Percentages vacuole (57%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Msb2

Msb2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msb2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available