Standard name
Description Mucin family member involved in various signaling pathways; functions as osmosensor in the Sho1p-mediated HOG pathway; functions in Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; processed into secreted and cell-associated forms by aspartyl protease Yps1p; potential Cdc28p substrate

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.05 0.09 0.07 0.12 0.12 0.15 0.21 0.28 0.31 0.47 0.49 0.7 0.16 0.2 0.23 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0.08 0.13 0 0 0 0 0 0 0 0.08 0.06 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.11 0.11 0.05 0 0.08 0.08 0.06 0.08 0.08 0.07 0 0 0 0 0.07 0 0 0 0 0 0 0
Nucleus 0.23 0.21 0.32 0.39 0.17 0.32 0.31 0.27 0.23 0.2 0.22 0.08 0.08 0.05 0.45 0.44 0.36 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.71 0.72 0.59 0.71 0.79 0.67 0.66 0.72 0.7 0.68 0.66 0.44 0.41 0.24 0.44 0.37 0.43 0.86 0.89 0.89 0.79 0.82 0.75
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 2 1 0 0 3 3 3 0 0 0 0 0 1 0 0 3
Bud 0 1 0 0 0 0 1 4 2 2 3 1 1 1 0 0 0 6 10 4 6 3 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 17 14 17 28 24 57 74 130 146 147 203 75 75 133 51 80 45 1 1 2 2 3 2
Endoplasmic Reticulum 1 1 3 1 3 5 2 3 2 4 2 1 2 4 8 16 12 1 2 0 2 2 2
Endosome 16 14 11 3 16 1 5 1 0 0 0 13 20 7 10 12 8 9 10 7 19 19 34
Golgi 2 5 1 0 3 0 0 1 0 0 0 0 0 0 8 0 3 3 1 2 1 3 3
Mitochondria 14 46 36 16 6 39 47 49 57 41 45 1 1 0 14 29 5 8 4 4 6 9 6
Nucleus 83 86 100 116 56 157 189 231 162 103 143 12 12 10 140 180 70 8 9 5 7 6 2
Nuclear Periphery 6 5 9 1 0 0 1 3 1 2 3 0 0 1 5 4 3 6 2 5 4 9 12
Nucleolus 12 13 6 0 2 1 4 5 2 2 0 0 0 0 0 5 3 4 3 0 0 0 1
Peroxisomes 1 1 0 0 2 0 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 1 0 0 1 1 1 2 1 0 5 3 1 5 10 0 2 1 0 1 0 0
Vac/Vac Membrane 255 288 188 214 263 326 403 610 492 356 429 70 63 45 137 151 82 352 398 321 206 272 248
Unique Cell Count 360 401 316 301 333 489 607 848 698 521 648 158 154 189 314 408 192 410 448 360 262 334 331
Labelled Cell Count 408 476 372 379 375 587 728 1041 868 658 829 181 180 209 378 487 231 410 448 360 262 334 331


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.3 10.8 9.6 9.9 13.5 12.5 12.5 12.4 11.1 10.9 10.0 7.2 7.2 6.7 8.3 8.2 10.0 10.4 11.4 11.9
Std Deviation (1e-4) 5.7 4.1 3.7 3.8 4.8 5.9 6.4 6.1 5.6 5.6 4.5 2.4 2.4 1.9 3.3 4.2 4.3 4.7 4.8 4.7
Intensity Change (Log2) 0.04 0.49 0.38 0.38 0.37 0.22 0.18 0.06 -0.4 -0.4 -0.53 -0.21 -0.23 0.06 0.12 0.24 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 1.0 3.0 3.3 4.5 6.2 8.1 9.0 10.9 11.1 15.5 4.4 5.6 6.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 1.5 2.5 3.4
Endosome -2.1 0.8 0 -2.9 0 0 0 0 2.2 3.9 0.1 -0.2 -0.4 0.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.7 -5.0 -1.6 -1.8 -3.3 -1.6 -1.7 -2.3 -4.1 -4.1 -4.8 -3.2 -2.0 -3.5
Nucleus 1.8 -4.4 0.1 -0.2 -1.5 -2.8 -3.9 -3.2 -5.8 -5.7 -6.9 3.3 3.4 1.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 -1.1 -1.9 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.0 5.4 2.1 2.1 4.1 3.4 2.6 2.0 -3.1 -3.8 -7.8 -4.0 -6.0 -3.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msb2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available