Standard name
Description cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.44 0.36 0.37 0.24 0.42 0.47 0.46 0.37 0.47 0.44 0.45 0.44 0.28 0.31 0.35 0.5 0.45 0.38 0.22 0.25 0.35 0.39 0.34 0.25
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0.15 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0.1 0 0 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.18 0.32 0.46 0.51 0.5 0.47 0.55 0.6 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.31 0.27 0.14 0.3 0.09 0.07 0.07 0.06 0.06 0.06 0.08 0.06 0.34 0.4 0.36 0.16 0.07 0.08 0.6 0.5 0.36 0.31 0.25 0.27
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.5 0.56 0.49 0.44 0.43 0.3 0.23 0.22 0.18 0.23 0.13 0.1 0.5 0.34 0.37 0.38 0.51 0.58 0.06 0.09 0.09 0.15 0.22 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 2
Bud 0 0 0 1 0 7 6 17 9 8 5 14 0 0 0 0 0 0 6 15 2 10 17 30
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 1 1 6
Bud Site 0 0 0 0 0 0 1 1 3 0 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 1 1 1 4 3 2 2 0 0 1 0 0 1 0 0 0 0 0 0
Cytoplasm 54 156 122 60 118 180 238 218 215 196 179 226 105 142 155 61 30 25 31 122 130 127 152 125
Endoplasmic Reticulum 1 3 2 0 0 1 0 3 0 0 1 1 2 2 3 1 4 2 1 1 2 2 3 6
Endosome 0 8 49 29 25 12 14 16 8 6 1 5 11 15 11 7 2 3 3 17 37 10 19 42
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 2 0 1 2 2 8 11
Mitochondria 0 3 12 16 51 123 236 301 230 210 218 304 1 1 0 4 2 2 0 1 2 3 6 11
Nucleus 39 119 46 77 26 27 37 37 28 25 33 29 128 181 159 19 5 5 83 241 134 98 112 137
Nuclear Periphery 0 1 3 0 0 0 2 2 4 2 9 2 0 0 1 0 0 0 0 2 3 1 0 2
Nucleolus 1 1 13 4 4 6 9 13 7 3 2 1 2 0 1 0 1 0 0 4 2 1 2 3
Peroxisomes 0 0 1 0 1 0 0 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 4 5 1 0 1 4 3 1 1 3 0 1 1 0 1 1 2 16 16 7 12 24
Vac/Vac Membrane 62 241 161 111 121 112 116 128 81 100 50 51 190 157 164 46 34 38 8 45 33 49 96 89
Unique Cell Count 124 433 328 255 283 379 515 586 456 443 397 508 378 456 444 122 67 66 139 484 375 322 444 503
Labelled Cell Count 157 533 413 303 348 469 661 744 593 554 503 640 440 500 496 138 79 79 139 484 375 322 444 503


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 7.4 6.5 6.5 5.7 5.2 4.3 4.5 4.4 4.2 4.2 4.2 8.4 7.5 7.8 8.2 7.8 9.0 9.0 8.9 8.6
Std Deviation (1e-4) 1.5 1.2 1.3 1.3 2.1 1.7 1.6 1.6 1.6 1.6 1.2 1.2 1.2 1.2 1.3 1.7 1.3 2.6 1.3 1.4 1.3
Intensity Change (Log2) – – – -0.01 -0.19 -0.33 -0.6 -0.55 -0.57 -0.62 -0.65 -0.65 0.36 0.21 0.26 0.33 0.26 0.46 0.47 0.44 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.5 1.1 2.8 2.6 0 2.8 2.0 2.1 2.1 -2.7 -1.8 -0.7 2.5 1.2 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.3 -2.3 -5.6 0 0 0 0 0 0 -5.7 -5.9 -6.4 -2.6 -2.7 -2.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.5 5.8 9.7 0 0 0 0 0 0 -3.3 -3.7 -4.1 0 0 0
Nucleus 4.7 -1.8 -3.0 -3.2 -3.9 -3.7 -4.0 -2.5 -4.1 6.1 7.8 6.8 0.4 -1.5 -1.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.7 -1.9 -2.0 0 0 0 0 0 0 -3.2 -4.3 -3.8 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.3 -1.6 -5.3 -8.0 -8.5 -9.4 -7.7 -10.8 -12.7 0.3 -4.1 -3.4 -2.2 0.2 1.3