Standard name
Description Nonessential component of the TIM23 complex; interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate the Translocase of the Inner Mitochondrial membrane (TIM23 complex) activity

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.11 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.08 0 0.06 0 0 0 0 0 0 0 0.15 0.09 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.06 0.07 0.13 0 0.09 0 0 0.07 0 0.1 0.09 0.08 0.11 0.07 0.08 0 0.07 0 0.06 0.08 0.06
Golgi 0 0.06 0.2 0.09 0.08 0 0 0 0 0 0 0.16 0.21 0.31 0.36 0.48 0.36 0.05 0.08 0.05 0.07 0.08 0.09
Mitochondria 0.86 0.89 0.77 0.82 0.64 0.9 0.69 0.9 0.88 0.9 0.93 0.8 0.76 0.65 0.14 0.1 0.07 0.78 0.74 0.81 0.71 0.65 0.64
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.06 0 0 0 0 0 0 0 0.06 0.05 0.07 0.05 0.07 0.07 0.05 0 0.06 0.07 0.06 0.07
SpindlePole 0 0 0.06 0 0.07 0 0.06 0 0.09 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.17 0.09 0.08 0.16 0.09 0.17 0.09 0.05 0.1 0.05 0.12 0.1 0.11 0.19 0.2 0.22 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 4 9 3 3 1 0 0 0 0 0 1 0 2 4 2 4 1 10 0 3 7 9
Bud 3 7 3 10 6 11 5 13 22 18 10 2 3 1 2 2 3 0 2 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 3 2
Bud Site 0 1 1 2 0 3 1 2 2 3 4 0 0 1 0 1 0
Cell Periphery 3 31 13 6 3 0 1 3 6 9 2 3 5 6 8 14 13 1 1 1 0 0 0
Cytoplasm 5 7 6 4 21 4 17 12 9 6 1 11 10 8 15 12 11 0 1 0 0 0 1
Endoplasmic Reticulum 3 10 4 0 3 4 1 4 2 0 3 2 5 3 4 1 3 0 2 0 0 0 5
Endosome 11 17 16 14 32 3 27 6 9 35 17 30 30 24 11 9 10 15 49 12 12 29 22
Golgi 13 38 55 18 21 1 5 1 2 0 2 49 71 90 37 64 43 15 59 15 13 28 32
Mitochondria 286 603 208 158 162 259 201 380 360 466 482 251 251 189 14 14 8 263 543 256 142 239 229
Nucleus 3 5 1 1 0 11 11 7 10 6 7 0 0 2 2 2 4 0 1 0 1 0 1
Nuclear Periphery 11 11 5 0 6 7 10 23 5 8 10 7 8 6 0 1 1 6 5 0 1 4 5
Nucleolus 14 10 1 1 4 10 5 15 8 17 7 13 5 16 0 0 3 1 1 0 0 3 1
Peroxisomes 4 13 3 11 4 0 4 4 7 11 6 19 15 21 5 9 9 16 17 18 13 22 25
SpindlePole 7 6 16 8 18 10 18 15 35 18 23 5 3 8 5 4 4 2 2 0 0 3 1
Vac/Vac Membrane 53 117 23 15 41 26 50 36 21 51 25 36 33 33 19 27 27 10 31 6 7 16 15
Unique Cell Count 331 681 270 192 255 289 292 422 411 517 521 313 331 290 102 134 121 339 733 316 201 366 359
Labelled Cell Count 416 880 364 251 324 350 356 521 499 648 599 429 439 410 126 162 143 339 733 316 201 366 359


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 6.5 6.7 6.6 7.3 4.8 5.9 5.3 5.1 5.3 5.3 7.9 8.0 8.0 8.7 9.8 8.7 6.6 6.9 7.0
Std Deviation (1e-4) 1.0 0.9 0.9 1.0 2.0 1.1 1.4 1.3 0.9 1.1 0.9 1.3 1.4 1.2 1.8 2.2 1.6 1.5 1.4 1.5
Intensity Change (Log2) -0.03 0.11 -0.5 -0.19 -0.34 -0.39 -0.35 -0.35 0.24 0.25 0.24 0.36 0.54 0.36 -0.02 0.03 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -1.7 0 0 0 0 0 0 0 0 -2.3 0 0 0
Bud 2.6 0 0 0 1.7 2.9 2.0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.9 -2.4 0 -3.4 -3.5 -2.6 -2.5 -4.3 -2.8 -2.4 -1.8 1.1 2.1 2.2
Cytoplasm 0 3.1 0 2.2 0.5 0 0 0 0.9 0.6 0.4 4.7 3.1 3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.6 2.6 0 1.5 -3.3 -2.5 0.5 -1.8 1.6 1.4 1.1 1.6 0.3 0.9
Golgi -3.2 -3.9 0 -7.2 -9.5 -9.2 -10.6 -10.3 -1.5 0.3 2.9 3.2 5.7 3.2
Mitochondria 1.4 -3.4 0 -2.2 4.7 3.6 5.0 6.2 0.9 -0.3 -3.1 -11.1 -12.7 -12.9
Nucleus 0 0 2.8 2.8 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.4 0 1.2 2.3 0 0 0.1 0.3 0.5 0.2 0 0 0
Nucleolus 0 0 0 0 2.7 0 2.6 0 3.0 0 3.5 0 0 0
Peroxisomes 2.9 0 0 0 0 0 0 0 3.1 2.4 3.6 0 0 0
SpindlePole -0.8 0.5 0 0.1 -1.5 1.3 -1.6 -0.9 -2.8 -3.5 -1.8 -0.4 -1.3 -1.1
Vacuole -0.3 2.6 0.2 3.0 0 -1.8 0.6 -2.1 1.2 0.6 1.1 2.7 3.3 3.8


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tim21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available