Standard name
Human Ortholog
Description Acyl-CoA-binding protein; transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 1.0 0.98 0.94 0.9 0.89 0.87 0.88 0.83 0.99 0.99 0.99 0.99 1.0 1.0 0.96 0.95 0.95 0.98 0.95 0.94
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.07 0.05 0.07 0.06 0.09 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 3 9 8 1 6 0 0 0 0 0 0 0 1 0 0 0 0
Bud 1 1 6 1 1 0 2 1 0 4 0 0 0 0 1 0 0 4 0 0 0 6 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 2 4 2 4 4 13 11 7 11 0 0 1 1 0 1 0 0 0 0 0 0
Cytoplasm 630 245 335 280 276 458 459 335 395 279 279 435 581 567 137 92 135 611 227 327 308 412 432
Endoplasmic Reticulum 2 1 1 0 7 26 35 20 34 18 30 1 3 1 3 5 4 1 0 2 0 1 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0
Golgi 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 4 3 8 14 13 0 0 0 0 1 0 0 1 1 1 0 0
Nucleus 0 0 0 0 0 1 2 4 3 0 4 0 0 0 0 0 0 5 3 2 0 3 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 2 2 0 0 0 0 5 2 11 3 2 2 2 1 0 0 1 1 0 0 1 3 5
Unique Cell Count 634 247 338 280 283 487 510 377 456 316 335 438 586 570 138 92 135 635 240 344 316 436 459
Labelled Cell Count 635 250 345 285 286 490 517 389 470 327 345 438 586 571 142 98 141 635 240 344 316 436 459


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 56.0 64.7 34.0 45.0 44.8 42.6 37.6 37.3 34.7 33.5 32.4 60.1 67.1 64.8 88.9 84.1 86.9 54.9 60.8 64.0
Std Deviation (1e-4) 12.5 13.7 9.6 11.1 10.7 9.4 10.1 9.7 9.2 9.4 9.2 14.6 15.6 15.0 34.8 33.6 28.5 13.3 16.5 18.6
Intensity Change (Log2) 0.4 0.4 0.32 0.14 0.13 0.03 -0.02 -0.07 0.82 0.98 0.93 1.38 1.3 1.35 0.69 0.84 0.91


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2.7 2.0 0 2.5 0 0 0 0 0 0
Cytoplasm 1.6 -1.6 -3.7 -5.3 -5.6 -6.4 -5.8 -7.3 0.3 0.1 0.6 0.2 0.9 1.1
Endoplasmic Reticulum 0 0 4.0 4.6 4.0 4.9 4.1 5.4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.9 3.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 45.4982 55.4203 52.5567 49.4758 49.2428 50.6224 42.4653 52.2168 44.3729 41.2516 36.3793 47.5345 40.2003 57.8806 50.9428 42.6708 45.938 51.6404
Actin 0.0005 0.0016 0.0001 0 0 0.0001 0.0092 0.0002 0.003 0.0005 0.0107 0.0006 0.0796 0.0001 0.0164 0.0033 0.0047 0.0001
Bud 0.0002 0.0003 0 0 0 0.0013 0.0002 0 0.0004 0.0002 0.0038 0.0001 0.0006 0 0 0.0005 0.0026 0.0001
Bud Neck 0.0002 0.0001 0.0002 0.0001 0.0002 0.0005 0.0003 0.0001 0.0002 0.0003 0.0005 0.0004 0.0007 0.0001 0.0001 0.0002 0.0003 0.0003
Bud Periphery 0.0002 0.0001 0 0 0 0.0004 0.0003 0 0.0006 0.0003 0.0029 0.0001 0.0006 0 0 0.0007 0.0026 0
Bud Site 0.0001 0.0002 0 0 0 0.0001 0.0006 0 0.001 0.0002 0.0008 0.0001 0.0034 0 0.0001 0.0026 0.0008 0
Cell Periphery 0.0003 0 0 0 0 0 0.0002 0 0.0005 0.0001 0.0005 0 0.0002 0 0 0.0008 0.0003 0
Cytoplasm 0.9527 0.9892 0.986 0.9818 0.983 0.9665 0.9064 0.9923 0.9407 0.9256 0.8282 0.9668 0.8 0.9962 0.9769 0.87 0.9033 0.989
Cytoplasmic Foci 0.0025 0.0001 0.0001 0.0001 0.0001 0.0001 0.0108 0.0001 0.0068 0.007 0.0104 0.0041 0.0133 0.0001 0.0001 0.0128 0.0035 0.0003
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0.0003 0.0001 0.0004 0 0.0006 0 0.0001 0.0003 0.0001 0
Endoplasmic Reticulum 0.0013 0.0002 0.0002 0.0001 0.0001 0.0004 0.0021 0.0003 0.0029 0.0007 0.0033 0.0006 0.0038 0 0.0003 0.0209 0.0073 0.0001
Endosome 0.001 0 0 0 0 0.0001 0.0027 0 0.0026 0.0022 0.0115 0.001 0.0162 0 0.0001 0.0157 0.0057 0.0001
Golgi 0.0002 0 0 0 0 0 0.0012 0 0.0009 0.0005 0.0098 0.0002 0.0117 0 0.0004 0.0016 0.0005 0
Lipid Particles 0.0022 0 0 0 0 0 0.0033 0 0.0024 0.0128 0.0097 0.0009 0.0149 0 0 0.0088 0.0005 0
Mitochondria 0.0004 0 0 0 0 0.0001 0.0076 0 0.0114 0.0141 0.0281 0.0004 0.0057 0 0 0.0061 0.0274 0.0001
None 0.0037 0.0001 0.0001 0.0001 0.0001 0.0001 0.0063 0.0001 0.0028 0.0015 0.0071 0.0011 0.0059 0.0001 0.0001 0.0211 0.0034 0.0001
Nuclear Periphery 0.0044 0.0008 0.0013 0.0015 0.0019 0.0033 0.0057 0.0008 0.0032 0.0032 0.0132 0.0018 0.0072 0.0003 0.0004 0.0082 0.01 0.0011
Nucleolus 0.0007 0 0 0 0 0.0001 0.0021 0 0.0015 0.0005 0.0021 0.0001 0.0003 0 0 0.0011 0.0005 0
Nucleus 0.0241 0.0068 0.0118 0.016 0.0141 0.0264 0.0304 0.0057 0.0126 0.0214 0.025 0.0174 0.0125 0.0031 0.0046 0.0095 0.0168 0.0084
Peroxisomes 0.0008 0 0 0 0 0 0.0022 0 0.0004 0.003 0.0013 0.0002 0.0159 0 0 0.0008 0.0007 0
Punctate Nuclear 0.0018 0.0001 0.0001 0.0001 0.0001 0.0001 0.005 0.0001 0.0006 0.0004 0.0016 0.0004 0.0058 0 0 0.0024 0.0007 0.0001
Vacuole 0.0022 0.0001 0.0002 0.0001 0.0002 0.0004 0.002 0.0001 0.0037 0.0028 0.0182 0.0032 0.0005 0 0 0.0102 0.0052 0.0001
Vacuole Periphery 0.0004 0 0 0 0 0.0001 0.001 0 0.0016 0.0027 0.0109 0.0003 0.0006 0 0 0.0024 0.0032 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 708.4358 496.9244 457.4586 432.3845 444.3568 614.0555 525.1062 549.465 466.4923 493.7426
Translational Efficiency 2.9167 3.2142 2.631 2.6209 3.1341 3.7278 2.8264 2.5927 2.7866 2.5454

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency