Standard name | |
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Human Ortholog | |
Description | Mitogen-activated protein kinase (MAPK); involved in signal transduction pathways that control filamentous growth and pheromone response; regulates septum assembly, and may directly phosphorylate Bni4p; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 5 | 6 |
Bud | 0 | 1 | 0 | 0 | 0 | 2 | 8 | 6 | 3 | 4 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 6 | 3 | 6 | 12 | 11 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 3 | 4 | 6 | 10 | 13 | 12 | 16 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 10 | 13 | 21 | 24 | 28 | 32 | 21 | 19 | 22 | 17 | 9 | 10 | 37 | 39 | 13 | 23 | 5 | 2 | 4 | 3 | 38 | 58 | 75 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 0 | 1 | 0 | 1 | 0 | 3 | 6 |
Endosome | 0 | 3 | 0 | 1 | 9 | 4 | 2 | 3 | 1 | 0 | 0 | 0 | 4 | 7 | 8 | 1 | 0 | 0 | 4 | 4 | 3 | 4 | 8 | 11 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 2 | 2 | 2 | 1 | 2 | 5 |
Mitochondria | 64 | 5 | 3 | 9 | 13 | 54 | 154 | 95 | 172 | 81 | 155 | 85 | 3 | 1 | 1 | 1 | 5 | 1 | 5 | 4 | 6 | 0 | 3 | 1 |
Nucleus | 237 | 169 | 138 | 200 | 316 | 273 | 307 | 421 | 259 | 315 | 227 | 304 | 99 | 103 | 93 | 59 | 59 | 18 | 232 | 189 | 133 | 20 | 33 | 36 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 |
Nucleolus | 13 | 7 | 1 | 0 | 0 | 5 | 13 | 17 | 20 | 16 | 30 | 16 | 5 | 7 | 0 | 1 | 2 | 0 | 20 | 16 | 7 | 2 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 3 | 5 | 5 | 2 | 1 | 4 | 3 | 4 | 0 | 1 | 1 | 3 | 2 | 0 | 0 | 0 | 3 | 3 | 4 | 5 | 5 | 7 |
Vac/Vac Membrane | 10 | 31 | 16 | 17 | 53 | 24 | 22 | 12 | 7 | 11 | 7 | 9 | 5 | 18 | 13 | 32 | 38 | 13 | 12 | 17 | 19 | 8 | 21 | 31 |
Unique Cell Count | 273 | 208 | 165 | 239 | 399 | 345 | 437 | 499 | 365 | 388 | 312 | 365 | 120 | 168 | 149 | 104 | 120 | 34 | 296 | 255 | 190 | 96 | 167 | 208 |
Labelled Cell Count | 332 | 226 | 176 | 254 | 425 | 398 | 548 | 590 | 497 | 469 | 460 | 441 | 127 | 177 | 159 | 109 | 135 | 37 | 296 | 255 | 190 | 96 | 167 | 208 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 7.4 | 6.4 | 6.4 | 6.4 | 5.5 | 5.2 | 5.8 | 4.7 | 5.6 | 4.8 | 5.5 | 7.9 | 8.2 | 7.7 | 9.5 | 10.0 | 9.0 | 7.8 | 8.6 | 9.1 |
Std Deviation (1e-4) | 0.6 | 1.2 | 1.2 | 1.3 | 1.1 | 1.3 | 1.3 | 1.3 | 1.1 | 1.4 | 1.1 | 1.0 | 3.3 | 2.9 | 2.4 | 1.5 | 2.4 | 1.5 | 2.6 | 3.2 | 3.5 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.02 | -0.23 | -0.31 | -0.15 | -0.45 | -0.19 | -0.41 | -0.23 | 0.29 | 0.36 | 0.25 | 0.56 | 0.64 | 0.48 | 0.28 | 0.41 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | -0.8 | 0.1 | -0.2 | -1.9 | -1.2 | -1.0 | -1.0 | -2.9 | 0.1 | 3.6 | 4.4 | 1.2 | 2.8 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.6 | 8.3 | 5.4 | 0 | 5.7 | 0 | 6.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | -1.2 | -1.2 | -3.3 | 0.2 | -3.1 | -0.7 | -2.7 | -0.1 | -0.3 | -4.6 | -4.3 | -4.9 | -6.2 | -4.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.9 | 1.2 | -1.1 | -2.1 | -4.0 | -4.1 | -3.4 | -3.6 | -3.6 | -1.8 | 0.3 | -0.3 | 4.4 | 4.7 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9692 | 4.6383 | 4.0759 | 3.6245 | 3.7475 | 4.2944 | 3.5978 | 4.2293 | 3.7272 | 3.4116 | 2.9619 | 3.6498 | 3.8872 | 3.4847 | 3.1963 | 2.942 | 3.2753 | 2.9013 |
Actin | 0.0376 | 0 | 0.0008 | 0.0476 | 0.0578 | 0.0025 | 0.0147 | 0.0003 | 0.0071 | 0.0077 | 0.0003 | 0.0015 | 0.017 | 0 | 0.02 | 0.0023 | 0.0045 | 0.001 |
Bud | 0.0005 | 0 | 0.0001 | 0.0041 | 0.0009 | 0.0002 | 0.001 | 0.0003 | 0.0004 | 0.0006 | 0.0004 | 0.0001 | 0.0008 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0002 |
Bud Neck | 0.0008 | 0.0001 | 0.0007 | 0.0023 | 0.002 | 0.0034 | 0.003 | 0.0004 | 0.0018 | 0.0093 | 0.0014 | 0.0018 | 0.003 | 0.0001 | 0.0007 | 0.0002 | 0.0008 | 0.0019 |
Bud Periphery | 0.0006 | 0 | 0.0003 | 0.0099 | 0.0011 | 0.0003 | 0.002 | 0.0003 | 0.0005 | 0.0012 | 0.001 | 0.0002 | 0.0036 | 0 | 0.0001 | 0.0002 | 0.0005 | 0.0003 |
Bud Site | 0.0025 | 0.0001 | 0.0007 | 0.0069 | 0.0042 | 0.0007 | 0.0116 | 0.0005 | 0.0027 | 0.0013 | 0.0006 | 0.0002 | 0.0011 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0002 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.006 | 0.0009 | 0.006 | 0.001 | 0.0056 | 0.0017 | 0.0032 | 0.0008 | 0.0073 | 0.0157 | 0.0074 | 0.0018 | 0.0165 | 0.0006 | 0.0043 | 0.0044 | 0.0042 | 0.0011 |
Cytoplasmic Foci | 0.0115 | 0 | 0.0014 | 0.0231 | 0.0137 | 0.0027 | 0.0144 | 0.0003 | 0.0056 | 0.0058 | 0.0032 | 0.0013 | 0.0047 | 0 | 0.0003 | 0.0021 | 0.0015 | 0.0029 |
Eisosomes | 0.0005 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0019 | 0 | 0.001 | 0.001 | 0.0029 | 0.0018 | 0.002 | 0.0002 | 0.0015 | 0.0025 | 0.0005 | 0.0003 | 0.0051 | 0 | 0.0008 | 0.0009 | 0.002 | 0.0008 |
Endosome | 0.0234 | 0 | 0.0043 | 0.0069 | 0.088 | 0.0206 | 0.0183 | 0.0009 | 0.0129 | 0.0176 | 0.0032 | 0.0035 | 0.0168 | 0 | 0.0017 | 0.0054 | 0.0139 | 0.0031 |
Golgi | 0.011 | 0 | 0.0005 | 0.0106 | 0.0256 | 0.005 | 0.0061 | 0.0001 | 0.0021 | 0.0105 | 0.0003 | 0.0024 | 0.0032 | 0 | 0.0004 | 0.0005 | 0.0085 | 0.0006 |
Lipid Particles | 0.0171 | 0 | 0.001 | 0.0125 | 0.0082 | 0.0033 | 0.0109 | 0.0001 | 0.0058 | 0.0051 | 0.002 | 0.0024 | 0.0016 | 0 | 0.0002 | 0.0003 | 0.0062 | 0.0002 |
Mitochondria | 0.0093 | 0.0001 | 0.0061 | 0.0164 | 0.0269 | 0.0146 | 0.0087 | 0.0005 | 0.0029 | 0.0113 | 0.0022 | 0.0084 | 0.004 | 0.0002 | 0.0004 | 0.0011 | 0.0155 | 0.001 |
None | 0.0047 | 0 | 0.0002 | 0.0005 | 0.0516 | 0.0015 | 0.0017 | 0.0001 | 0.0005 | 0.0021 | 0.0145 | 0.0002 | 0.002 | 0 | 0.0005 | 0.0283 | 0.0006 | 0.0032 |
Nuclear Periphery | 0.0081 | 0.0022 | 0.0028 | 0.0034 | 0.0096 | 0.0054 | 0.014 | 0.0032 | 0.0071 | 0.0096 | 0.0082 | 0.0022 | 0.0297 | 0.0021 | 0.012 | 0.0075 | 0.0437 | 0.0053 |
Nucleolus | 0.0055 | 0.0107 | 0.0059 | 0.0052 | 0.0435 | 0.018 | 0.0117 | 0.0057 | 0.0071 | 0.0101 | 0.0207 | 0.0165 | 0.0085 | 0.0054 | 0.0068 | 0.0023 | 0.0086 | 0.0103 |
Nucleus | 0.8365 | 0.9848 | 0.9579 | 0.8057 | 0.6189 | 0.9117 | 0.8361 | 0.9716 | 0.9026 | 0.8387 | 0.9084 | 0.9489 | 0.8556 | 0.9771 | 0.9367 | 0.939 | 0.8755 | 0.9517 |
Peroxisomes | 0.0076 | 0 | 0.0056 | 0.0332 | 0.0268 | 0.0007 | 0.0091 | 0.0001 | 0.0098 | 0.0024 | 0.0017 | 0.0012 | 0.0005 | 0 | 0.0001 | 0.0007 | 0.0005 | 0.0001 |
Punctate Nuclear | 0.0122 | 0.0007 | 0.0007 | 0.0065 | 0.007 | 0.0042 | 0.0206 | 0.014 | 0.0126 | 0.0456 | 0.0221 | 0.0064 | 0.0169 | 0.0141 | 0.0138 | 0.0039 | 0.0082 | 0.015 |
Vacuole | 0.0014 | 0.0001 | 0.0031 | 0.0016 | 0.0034 | 0.0011 | 0.0083 | 0.0004 | 0.0072 | 0.0016 | 0.0013 | 0.0003 | 0.0063 | 0.0001 | 0.0003 | 0.0003 | 0.002 | 0.0008 |
Vacuole Periphery | 0.0009 | 0 | 0.0006 | 0.0009 | 0.0021 | 0.0006 | 0.0022 | 0.0001 | 0.0022 | 0.0008 | 0.0005 | 0.0003 | 0.0025 | 0 | 0.0004 | 0.0003 | 0.0024 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.948 | 4.4552 | 11.9014 | 16.6562 | 17.4465 | 7.9696 | 14.64 | 12.7693 | 15.0767 | 19.7661 |
Translational Efficiency | 0.6671 | 2.2799 | 0.8284 | 0.732 | 0.8082 | 1.1287 | 0.7339 | 0.6862 | 0.686 | 0.7134 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
36 | 695 | 2936 | 1124 | 1948 | 1804 | 1164 | 1366 | 1984 | 2499 | 4100 | 2490 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1194.16 | 895.19 | 987.88 | 927.81 | 769.38 | 846.88 | 978.08 | 999.02 | 777.09 | 860.32 | 985.10 | 966.88 |
Standard Deviation | 725.92 | 135.73 | 126.78 | 146.02 | 104.55 | 118.35 | 119.20 | 137.35 | 153.33 | 125.31 | 124.75 | 145.70 |
Intensity Change Log 2 | — | -0.415730 | -0.273588 | -0.364095 | — | 0.138461 | 0.346256 | 0.376817 | — | -0.172654 | 0.001777 | -0.027228 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002029 | 0.001117 | 0.000507 | 0.001050 | 0.000247 | 0.000695 | 0.000863 | 0.002553 | 0.000280 | 0.000812 | 0.000608 | 0.001875 |
Bud Neck | 0.048090 | 0.014384 | 0.023847 | 0.021251 | 0.004733 | 0.013644 | 0.011399 | 0.030510 | 0.005519 | 0.013850 | 0.020313 | 0.026330 |
Bud Site | 0.026721 | 0.005738 | 0.002987 | 0.013085 | 0.000557 | 0.001949 | 0.001964 | 0.039057 | 0.001032 | 0.003003 | 0.002696 | 0.027333 |
Cell Periphery | 0.000323 | 0.000249 | 0.000099 | 0.000260 | 0.000089 | 0.000102 | 0.000138 | 0.000192 | 0.000093 | 0.000143 | 0.000110 | 0.000223 |
Cytoplasm | 0.020473 | 0.020184 | 0.010933 | 0.079481 | 0.016434 | 0.013673 | 0.027136 | 0.037873 | 0.016507 | 0.015483 | 0.015533 | 0.056655 |
Cytoplasmic Foci | 0.011447 | 0.001940 | 0.000180 | 0.004831 | 0.001228 | 0.002991 | 0.000910 | 0.010206 | 0.001414 | 0.002699 | 0.000387 | 0.007780 |
Eisosomes | 0.000079 | 0.000030 | 0.000019 | 0.000042 | 0.000016 | 0.000025 | 0.000036 | 0.000038 | 0.000017 | 0.000026 | 0.000024 | 0.000040 |
Endoplasmic Reticulum | 0.023000 | 0.006998 | 0.003260 | 0.007905 | 0.002486 | 0.004526 | 0.007128 | 0.007073 | 0.002858 | 0.005213 | 0.004358 | 0.007448 |
Endosome | 0.004435 | 0.001084 | 0.000472 | 0.009377 | 0.000352 | 0.001439 | 0.000662 | 0.021400 | 0.000426 | 0.001340 | 0.000526 | 0.015973 |
Golgi | 0.000852 | 0.000343 | 0.000091 | 0.002999 | 0.000170 | 0.001533 | 0.000091 | 0.013654 | 0.000183 | 0.001202 | 0.000091 | 0.008844 |
Lipid Particles | 0.020346 | 0.001221 | 0.000089 | 0.000951 | 0.000577 | 0.000712 | 0.000513 | 0.001106 | 0.000936 | 0.000853 | 0.000210 | 0.001036 |
Mitochondria | 0.004360 | 0.002720 | 0.001615 | 0.006854 | 0.001234 | 0.003509 | 0.001618 | 0.010096 | 0.001290 | 0.003290 | 0.001616 | 0.008632 |
Mitotic Spindle | 0.005130 | 0.009418 | 0.002886 | 0.046983 | 0.002143 | 0.008312 | 0.003549 | 0.158001* | 0.002198 | 0.008620 | 0.003074 | 0.107887* |
None | 0.019180 | 0.008173 | 0.004466 | 0.011788 | 0.006356 | 0.005660 | 0.007693 | 0.010404 | 0.006589 | 0.006359 | 0.005382 | 0.011028 |
Nuclear Periphery | 0.002681 | 0.001527 | 0.001127 | 0.002792 | 0.000918 | 0.001232 | 0.001204 | 0.004392 | 0.000950 | 0.001314 | 0.001149 | 0.003670 |
Nuclear Periphery Foci | 0.018553 | 0.001696 | 0.000164 | 0.001980 | 0.000887 | 0.000959 | 0.000468 | 0.004268 | 0.001207 | 0.001164 | 0.000250 | 0.003235 |
Nucleolus | 0.036848 | 0.017883 | 0.005495 | 0.008655 | 0.016962 | 0.023564 | 0.005299 | 0.007460 | 0.017323 | 0.021984 | 0.005440 | 0.008000 |
Nucleus | 0.733126* | 0.901271* | 0.939856* | 0.752293* | 0.943526* | 0.912592* | 0.921446* | 0.599146* | 0.939708* | 0.909444* | 0.934629* | 0.668277* |
Peroxisomes | 0.001778 | 0.001481 | 0.000103 | 0.001423 | 0.000416 | 0.001074 | 0.000212 | 0.001970 | 0.000441 | 0.001187 | 0.000134 | 0.001723 |
Vacuole | 0.016883 | 0.002265 | 0.001691 | 0.022352 | 0.000549 | 0.001335 | 0.007520 | 0.033340 | 0.000845 | 0.001593 | 0.003346 | 0.028380 |
Vacuole Periphery | 0.003666 | 0.000279 | 0.000114 | 0.003645 | 0.000118 | 0.000475 | 0.000153 | 0.007261 | 0.000182 | 0.000421 | 0.000125 | 0.005629 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.31 | 2.25 | 0.50 | -3.37 | -11.44 | -6.10 | -14.64 | -8.32 | -5.56 | -3.72 | -8.25 | -12.27 | -12.48 | -7.02 | -8.15 |
Bud Neck | 1.25 | 0.62 | 0.41 | -7.07 | -1.92 | -8.87 | -9.48 | -20.60 | -15.86 | -14.57 | -9.20 | -20.29 | -23.26 | -17.03 | -10.85 |
Bud Site | 1.16 | 1.33 | 0.66 | -3.89 | -7.14 | -7.01 | -9.18 | -12.59 | -11.82 | -11.27 | -4.04 | -4.48 | -14.73 | -12.85 | -13.09 |
Cell Periphery | 0.68 | 2.73 | 1.28 | 0.70 | -9.29 | -1.28 | -4.08 | -7.93 | -6.40 | -3.98 | -2.79 | -1.08 | -9.63 | -2.53 | -11.16 |
Cytoplasm | 1.20 | 1.59 | 0.50 | -5.17 | -10.02 | 1.67 | -0.94 | -6.02 | -7.28 | -4.15 | 1.02 | 3.54 | -8.05 | -9.29 | -12.34 |
Cytoplasmic Foci | 1.15 | 1.37 | 1.12 | -0.33 | -4.19 | -2.19 | 2.44 | -3.91 | -0.63 | -5.30 | -1.95 | 4.46 | -3.15 | -0.36 | -7.21 |
Eisosomes | 2.60 | 3.17 | 0.64 | -8.98 | -14.88 | -5.66 | -13.24 | -18.05 | -12.75 | -2.96 | -6.61 | -10.20 | -22.68 | -16.23 | -15.83 |
Endoplasmic Reticulum | 2.23 | 2.77 | 1.73 | -4.26 | -13.18 | -8.67 | -16.32 | -16.46 | -9.81 | 0.40 | -9.59 | -13.05 | -21.00 | -11.55 | -11.74 |
Endosome | 1.71 | 2.13 | -0.09 | -3.38 | -4.34 | -3.36 | -7.00 | -6.45 | -5.04 | -5.84 | -3.70 | -1.97 | -7.73 | -5.91 | -7.45 |
Golgi | 1.30 | 2.00 | -0.68 | -2.10 | -2.78 | -3.06 | 0.68 | -3.06 | -1.51 | -3.11 | -3.13 | 1.09 | -3.70 | -1.63 | -3.82 |
Lipid Particles | 1.74 | 1.85 | 1.77 | 0.88 | -10.11 | -1.04 | -0.10 | -4.36 | -3.37 | -5.82 | 0.06 | 2.98 | -0.90 | -1.53 | -12.39 |
Mitochondria | 0.71 | 1.17 | -0.20 | -2.12 | -3.96 | -3.27 | -1.44 | -5.53 | -2.44 | -5.41 | -3.35 | -1.55 | -5.97 | -2.87 | -6.78 |
Mitotic Spindle | -1.63 | 0.12 | -9.29 | -8.32 | -10.75 | -3.72 | -3.01 | -19.23 | -18.10 | -18.18 | -4.56 | -2.89 | -21.71 | -19.73 | -21.22 |
None | 0.67 | 0.91 | 0.59 | -0.97 | -5.78 | 1.28 | 0.83 | -2.54 | -3.74 | -3.09 | 0.73 | 2.94 | -2.73 | -3.95 | -6.88 |
Nuclear Periphery | 0.51 | 0.78 | -1.53 | -6.06 | -10.89 | -2.22 | -6.66 | -13.54 | -11.64 | -10.28 | -3.21 | -6.76 | -17.85 | -13.88 | -14.73 |
Nuclear Periphery Foci | 1.70 | 1.94 | 1.52 | -1.78 | -5.13 | -0.72 | -0.32 | -5.78 | -5.57 | -5.76 | -0.68 | 2.14 | -5.79 | -5.80 | -8.26 |
Nucleolus | 1.78 | 2.87 | 1.66 | -0.40 | -5.18 | -2.89 | 6.43 | -5.35 | -3.31 | -9.80 | -2.20 | 6.68 | -4.09 | -2.38 | -10.86 |
Nucleus | -2.99 | -3.58 | -0.59 | 12.95 | 19.73 | 6.26 | 6.55 | 33.78 | 29.49 | 28.77 | 6.80 | 4.27 | 36.22 | 31.14 | 35.90 |
Peroxisomes | 0.28 | 1.96 | 1.04 | 0.92 | -3.01 | -2.90 | 1.28 | -4.15 | -1.38 | -5.71 | -3.05 | 1.25 | -3.92 | -0.17 | -5.77 |
Vacuole | 2.06 | 2.15 | -2.02 | -11.08 | -11.40 | -4.49 | -8.28 | -15.09 | -14.66 | -9.14 | -4.92 | -9.25 | -19.11 | -18.41 | -16.00 |
Vacuole Periphery | 1.64 | 1.72 | 1.12 | -2.44 | -2.88 | -4.61 | -3.25 | -3.68 | -2.79 | -3.56 | -3.65 | 1.16 | -4.44 | -3.56 | -4.62 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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