Standard name
Description Mitogen-activated protein kinase (MAPK); involved in signal transduction pathways that control filamentous growth and pheromone response; regulates septum assembly, and may directly phosphorylate Bni4p; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.08 0.09 0.06 0.08 0.07 0 0.05 0.06 0.05 0 0.08 0.22 0.26 0.12 0.19 0.15 0 0 0 0.4 0.35 0.36
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0 0 0 0 0.16 0.35 0.19 0.47 0.21 0.5 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.87 0.81 0.84 0.84 0.79 0.79 0.7 0.84 0.71 0.81 0.73 0.83 0.82 0.61 0.62 0.57 0.49 0.53 0.78 0.74 0.7 0.22 0.2 0.18
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 0 0.05 0 0.1 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Vac/Vac Membrane 0 0.15 0.1 0.07 0.13 0.07 0.05 0 0 0 0 0 0 0.11 0.09 0.31 0.32 0.38 0 0.07 0.1 0.09 0.13 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 5 6
Bud 0 1 0 0 0 2 8 6 3 4 4 4 0 0 0 0 0 0 4 6 3 6 12 11
Bud Neck 0 0 1 0 0 1 1 0 0 3 2 1 0 0 0 0 0 0 0 0 0 1 4 6
Bud Site 0 0 0 0 3 4 6 10 13 12 16 12 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 10 13 21 24 28 32 21 19 22 17 9 10 37 39 13 23 5 2 4 3 38 58 75
Endoplasmic Reticulum 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 1 7 0 1 0 1 0 3 6
Endosome 0 3 0 1 9 4 2 3 1 0 0 0 4 7 8 1 0 0 4 4 3 4 8 11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 0 2 2 2 1 2 5
Mitochondria 64 5 3 9 13 54 154 95 172 81 155 85 3 1 1 1 5 1 5 4 6 0 3 1
Nucleus 237 169 138 200 316 273 307 421 259 315 227 304 99 103 93 59 59 18 232 189 133 20 33 36
Nuclear Periphery 0 0 0 0 0 0 2 1 0 1 2 0 0 0 1 0 0 0 1 1 0 0 1 0
Nucleolus 13 7 1 0 0 5 13 17 20 16 30 16 5 7 0 1 2 0 20 16 7 2 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
SpindlePole 0 0 3 5 5 2 1 4 3 4 0 1 1 3 2 0 0 0 3 3 4 5 5 7
Vac/Vac Membrane 10 31 16 17 53 24 22 12 7 11 7 9 5 18 13 32 38 13 12 17 19 8 21 31
Unique Cell Count 273 208 165 239 399 345 437 499 365 388 312 365 120 168 149 104 120 34 296 255 190 96 167 208
Labelled Cell Count 332 226 176 254 425 398 548 590 497 469 460 441 127 177 159 109 135 37 296 255 190 96 167 208


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 7.4 6.4 6.4 6.4 5.5 5.2 5.8 4.7 5.6 4.8 5.5 7.9 8.2 7.7 9.5 10.0 9.0 7.8 8.6 9.1
Std Deviation (1e-4) 0.6 1.2 1.2 1.3 1.1 1.3 1.3 1.3 1.1 1.4 1.1 1.0 3.3 2.9 2.4 1.5 2.4 1.5 2.6 3.2 3.5
Intensity Change (Log2) -0.01 -0.02 -0.23 -0.31 -0.15 -0.45 -0.19 -0.41 -0.23 0.29 0.36 0.25 0.56 0.64 0.48 0.28 0.41 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -0.8 0.1 -0.2 -1.9 -1.2 -1.0 -1.0 -2.9 0.1 3.6 4.4 1.2 2.8 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.6 8.3 5.4 0 5.7 0 6.2 0 0 0 0 0 0
Nucleus 0 -1.2 -1.2 -3.3 0.2 -3.1 -0.7 -2.7 -0.1 -0.3 -4.6 -4.3 -4.9 -6.2 -4.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 2.2 0 2.3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.9 1.2 -1.1 -2.1 -4.0 -4.1 -3.4 -3.6 -3.6 -1.8 0.3 -0.3 4.4 4.7 0