Standard name
Description Protein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole; BUD9 has a paralog, BUD8, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.09 0.06 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0.06 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.77 0.87 0.87 0.92 0.87 0.76 0.73 0.73 0.61 0.6 0.55 0.5 0.98 0.99 1.0 0.84 0.86 0.8 0.44 0.52 0.38 0.67 0.75 0.79
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.09 0.05 0 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0 0.08 0.08 0.13 0.35 0.43 0.43 0.59 0.6 0.56 0.72 0 0 0 0.05 0 0 0.18 0.13 0.27 0.1 0.09 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 8 7 1 0 0 0 0 0 0 0 0 0 0 0 0 1 4 11 12 32 11 7 5
Bud 2 3 11 3 5 7 10 8 15 8 4 1 2 1 0 3 3 7 16 19 20 7 3 5
Bud Neck 0 3 4 0 0 1 1 3 1 3 0 1 0 0 0 4 4 3 7 13 11 7 4 3
Bud Site 3 2 2 1 1 0 1 4 2 1 1 0 0 0 0 2 0 0 – – – – – –
Cell Periphery 1 2 4 4 0 0 4 1 4 1 0 1 0 1 3 1 3 7 2 3 5 3 3 3
Cytoplasm 75 193 310 209 253 210 321 298 223 249 70 81 344 393 438 283 179 132 82 116 140 137 183 184
Endoplasmic Reticulum 1 11 10 0 0 0 0 0 7 1 0 1 6 2 0 19 16 15 10 8 22 3 6 3
Endosome 1 4 1 1 8 3 2 0 1 1 0 0 0 1 0 7 3 5 5 1 5 2 2 1
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 2 0 1 1 1 1
Mitochondria 26 4 27 18 37 98 190 177 217 249 71 116 0 1 2 18 6 7 33 29 99 20 21 11
Nucleus 0 0 0 0 1 0 1 0 1 0 0 1 0 0 2 6 0 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 1 1 1 0 0 0 0 2 0 0 0 0 0 0 0 0
Peroxisomes 1 1 2 3 3 0 1 0 0 1 0 0 0 0 0 6 2 1 1 0 3 0 1 0
SpindlePole 3 2 3 9 4 5 7 6 7 6 7 2 1 0 0 20 6 5 4 5 7 4 6 2
Vac/Vac Membrane 4 4 1 1 6 7 3 7 2 1 2 2 1 0 0 10 13 8 7 13 10 2 2 6
Unique Cell Count 98 221 355 227 291 278 437 407 367 416 127 162 350 398 439 336 207 164 190 226 369 205 246 235
Labelled Cell Count 117 237 384 250 318 331 542 506 481 522 156 206 355 400 446 382 236 196 190 226 369 205 246 235


Ambiguous, Bud, Bud Neck

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.3 3.9 3.5 3.8 3.2 2.6 2.9 2.8 2.6 2.7 2.6 4.4 4.2 4.2 5.3 5.9 6.1 3.8 4.0 4.8
Std Deviation (1e-4) 0.7 0.7 1.1 1.3 1.8 0.9 0.9 1.0 0.6 1.1 1.2 1.0 1.2 1.3 1.2 1.6 1.5 2.4 1.1 1.4 1.4
Intensity Change (Log2) – – – -0.13 -0.01 -0.29 -0.58 -0.41 -0.48 -0.56 -0.53 -0.57 0.17 0.12 0.1 0.46 0.61 0.66 -0.02 0.05 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 2.8 4.4 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.76 0.0002 0.0055 0.0012 0 0.0007 0.1762 0.0114 0.0056 0.0005 0.0057 0.0145 0.0717 0.0002 0.0816 0.0056 0.0101 0.0123
Bud 0.0034 0.0002 0.0083 0.0017 0 0.0001 0.0015 0.0001 0.0082 0.0008 0.0068 0.0076 0.0017 0.0006 0.0211 0.0011 0.0074 0.0004
Bud Neck 0.0037 0.008 0.0005 0.0078 0 0.0017 0.0285 0.0003 0.0054 0.0001 0.0002 0.0199 0.0235 0.0029 0.0054 0.0005 0.0007 0.0476
Bud Periphery 0.0045 0.0002 0.0403 0.001 0 0.0002 0.0026 0.0002 0.0258 0.0011 0.0286 0.017 0.0048 0.0009 0.0744 0.0018 0.0257 0.0006
Bud Site 0.0269 0.1565 0.0277 0.0484 0 0.0001 0.0119 0.0008 0.004 0.0002 0.001 0.0015 0.013 0.0606 0.0099 0.0073 0.0087 0.0006
Cell Periphery 0.001 0.0011 0.0018 0.0002 0 0.0003 0.0009 0.0005 0.0007 0.0002 0.0003 0.0008 0.0017 0.0017 0.0008 0.0003 0.0011 0.0007
Cytoplasm 0.0052 0.035 0.0824 0.0196 0.0084 0.1407 0.1158 0.1203 0.0569 0.271 0.0869 0.1325 0.0838 0.099 0.0556 0.0937 0.0378 0.0932
Cytoplasmic Foci 0.0589 0.0011 0.0581 0.051 0.0003 0.0092 0.1481 0.0193 0.0409 0.0163 0.0144 0.0074 0.0188 0.0188 0.0233 0.0883 0.0336 0.0196
Eisosomes 0.0023 0.0005 0.002 0.0001 0.0001 0.0003 0.0013 0.0009 0.002 0.0002 0.0002 0.0002 0.001 0.0004 0.0007 0.0002 0.0006 0.0002
Endoplasmic Reticulum 0.0039 0.0026 0.0037 0.0001 0.0002 0.0026 0.0079 0.0028 0.0025 0.0039 0.0011 0.0031 0.0013 0.0007 0.0014 0.0012 0.0018 0.0014
Endosome 0.0174 0.0003 0.0072 0.0096 0 0.002 0.0577 0.0132 0.0276 0.0332 0.0053 0.0014 0.004 0.0013 0.008 0.0102 0.0233 0.0029
Golgi 0.0368 0.0001 0.0021 0.0056 0 0.0003 0.025 0.0258 0.0129 0.0013 0.003 0.0013 0.0029 0.0005 0.0092 0.0041 0.0054 0.0016
Lipid Particles 0.0352 0.0004 0.0049 0.0016 0 0.0015 0.0995 0.019 0.0054 0.001 0.0037 0.0013 0.0307 0.0016 0.0023 0.0047 0.0266 0.0008
Mitochondria 0.0057 0.0005 0.003 0.0005 0 0.0005 0.0111 0.0032 0.0346 0.0016 0.0062 0.0242 0.0011 0.0003 0.0086 0.0034 0.0017 0.0006
None 0.003 0.7921 0.7321 0.8393 0.9908 0.8362 0.2203 0.7716 0.7451 0.6562 0.8124 0.7551 0.713 0.77 0.6843 0.7267 0.7756 0.8142
Nuclear Periphery 0.0014 0.0003 0.0008 0 0 0.0004 0.0292 0.0005 0.0011 0.0024 0.0021 0.0011 0.0005 0.0002 0.0004 0.0005 0.0019 0.0004
Nucleolus 0.0003 0.0001 0.0009 0.0004 0 0.0001 0.0098 0.0002 0.0007 0.0002 0.0006 0.0004 0.0006 0.0016 0.0004 0.0004 0.0014 0.0002
Nucleus 0.0004 0.0002 0.0007 0.0002 0 0.0004 0.0026 0.0003 0.0009 0.0012 0.0005 0.0011 0.0006 0.0009 0.0005 0.0006 0.0018 0.0003
Peroxisomes 0.0242 0.0001 0.0097 0.0097 0 0.0013 0.032 0.0081 0.0093 0.0005 0.0153 0.0063 0.0216 0.0012 0.0077 0.0425 0.0167 0.0007
Punctate Nuclear 0.0045 0.0001 0.0032 0.0008 0 0.0007 0.0084 0.0009 0.0059 0.0007 0.003 0.0018 0.0031 0.0347 0.0021 0.0056 0.0156 0.0014
Vacuole 0.0008 0.0004 0.0044 0.0012 0 0.0006 0.0049 0.0006 0.0033 0.0054 0.0015 0.0012 0.0006 0.0016 0.0018 0.0011 0.0022 0.0003
Vacuole Periphery 0.0004 0.0001 0.0008 0.0001 0 0.0001 0.0047 0.0002 0.0013 0.002 0.001 0.0004 0.0001 0.0001 0.0006 0.0003 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.732 4.7999 7.5687 27.6771 27.8465 7.3788 4.5498 4.5039 15.0677 45.2101
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Abundance –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –