Standard name
Description High affinity methionine permease; integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0.07 0.05 0 0 0.1 0.21 0.21 0.34 0.45 0 0 0.05 0.14 0 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.11 0.24 0 0.1 – 0 0 0 0 0.07 0.12
Golgi 0 0 0 0 0.05 0 0 0 0 0 0 0 0.08 0.08 0 0.11 0.15 – 0.06 0 0.08 0.14 0.12 0.15
Mitochondria 0.12 0.25 0.21 0 0.07 0.12 0.05 0.06 0.07 0.06 0.1 0.06 0.09 0.08 0 0 0.05 – 0.05 0 0.11 0.08 0.11 0.13
Nucleus 0.15 0.14 0.12 0.2 0.12 0.23 0.27 0.17 0.17 0.18 0.09 0.1 0.07 0.07 0.08 0.23 0.25 – 0.06 0.05 0 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0.11 0.13 0.11 0.06 0 0.07 0.05 0.06 0 0 0 0 0.09 0.09 0.1 0.12 0.2 – 0.06 0 0.05 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0.18 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0.68 0.66 0.69 0.71 0.77 0.67 0.66 0.71 0.67 0.68 0.61 0.51 0.67 0.68 0.55 0.62 0.5 – 0.71 0.79 0.67 0.62 0.59 0.49
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 2 1 0 3 4 2 1 2 5 4 3 4 0 0 – 0 1 1 2 1 6
Bud 1 0 0 1 0 1 0 3 4 5 7 9 3 3 5 0 0 – 1 0 1 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 0 1 2 2 1 0 0 1 0 0 – – – – – – –
Cell Periphery 0 0 0 2 0 0 0 1 1 0 1 2 1 1 1 0 0 – 0 0 0 0 0 0
Cytoplasm 14 0 11 21 22 8 29 77 111 131 112 250 17 17 33 8 0 – 2 0 0 0 0 2
Endoplasmic Reticulum 0 0 1 4 1 2 4 4 5 6 2 4 0 0 0 2 1 – 0 0 0 0 0 2
Endosome 0 0 2 0 3 14 25 29 18 27 8 23 76 75 165 1 2 – 8 3 6 9 24 44
Golgi 11 1 3 6 22 1 6 2 3 1 0 1 53 54 22 6 3 – 19 3 27 30 40 57
Mitochondria 31 27 61 8 30 44 35 50 35 36 34 34 60 60 28 2 1 – 14 5 35 17 35 48
Nucleus 40 15 33 63 50 86 173 132 90 112 30 54 48 48 58 13 5 – 18 6 13 9 6 5
Nuclear Periphery 3 1 0 0 1 3 4 2 0 0 2 1 3 5 2 0 0 – 2 1 3 2 7 9
Nucleolus 28 14 31 17 18 26 33 48 18 24 8 19 65 65 69 7 4 – 20 5 15 6 8 4
Peroxisomes 0 0 0 0 2 3 4 8 5 0 3 2 21 23 28 0 0 – 0 0 1 0 2 3
SpindlePole 0 0 0 0 2 6 20 19 14 15 11 18 62 61 123 0 0 – 0 0 1 1 2 2
Vac/Vac Membrane 177 72 196 220 321 247 431 558 346 420 204 283 464 480 385 35 10 – 223 104 220 134 191 183
Unique Cell Count 262 109 285 308 417 368 649 783 519 619 334 560 697 710 697 56 20 316 132 330 216 324 376
Labelled Cell Count 305 130 338 344 473 442 767 937 653 780 426 706 877 895 924 74 26 316 132 330 216 324 376


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 44.8 44.1 43.9 44.2 39.4 27.9 23.5 19.8 17.8 15.8 13.5 13.4 15.4 15.4 11.3 47.9 38.0 – 34.4 29.1 27.6
Std Deviation (1e-4) 19.5 18.1 18.2 17.8 18.5 18.1 16.2 15.0 13.4 13.4 11.7 10.9 6.0 6.0 3.9 29.4 20.0 – 15.0 12.9 11.8
Intensity Change (Log2) – – – 0.01 -0.15 -0.65 -0.9 -1.15 -1.3 -1.47 -1.7 -1.71 -1.51 -1.51 -1.96 0.13 -0.21 – -0.35 -0.59 -0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cytoplasm 1.6 0.9 -1.3 0.4 3.1 6.6 6.6 9.2 12.1 -1.2 -1.3 0.6 0 0 –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Endosome 0 0 2.5 2.6 2.6 2.4 2.9 0 2.8 5.4 5.3 8.7 0 0 –
Golgi 0 3.0 0 0 0 0 0 0 0 4.0 4.0 1.9 0 0 –
Mitochondria -7.1 -5.5 -3.3 -7.4 -7.1 -6.1 -7.0 -3.9 -6.7 -5.5 -5.7 -8.6 -3.1 0 –
Nucleus 2.9 0.2 3.9 5.1 2.1 2.2 2.5 -1.1 -0.9 -2.4 -2.5 -1.6 2.3 0 –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nucleolus -2.4 -3.4 -1.7 -3.2 -2.6 -4.2 -4.1 -4.3 -4.4 -0.7 -0.8 -0.5 0.4 0 –
Peroxisomes 0 0 0 0 0 0 0 0 0 3.0 3.1 3.4 0 0 –
SpindlePole 0 0 0 3.0 2.7 0 0 3.1 3.1 5.2 5.1 7.6 0 0 –
Vacuole 0.7 2.4 -0.4 -0.7 0.8 -0.6 -0.3 -2.0 -5.1 -0.7 -0.4 -3.9 -0.9 -1.7 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 37.9565 63.9243 58.6418 45.5666 56.4469 61.1304 43.2528 58.1299 57.6054 50.6654 53.5988 61.877 50.1976 75.6191 67.0112 62.4065 65.5332 67.9858
Actin 0.0052 0 0 0 0 0 0.0008 0.0001 0.0001 0.0001 0 0.0001 0.0018 0 0.0001 0 0 0
Bud 0.0002 0.0001 0.0001 0.0011 0.0005 0.0001 0.0003 0.0004 0.0002 0.0004 0.0001 0.0001 0.0004 0 0.0001 0.0001 0.0003 0
Bud Neck 0.0004 0.0004 0.0004 0.001 0.0004 0.0002 0.0004 0.0011 0.0003 0.0046 0.0002 0.0005 0.0003 0.0001 0.0013 0.0004 0.0003 0.0002
Bud Periphery 0.0005 0.0003 0.0003 0.0007 0.0011 0.0003 0.0005 0.0005 0.0003 0.0011 0.0006 0.0005 0.0011 0.0002 0.0003 0.0005 0.0013 0.0002
Bud Site 0.0008 0.0002 0.0001 0.0004 0.0002 0 0.0006 0.0016 0.0005 0.0011 0.0001 0.0001 0.0005 0 0.0002 0.0003 0.0003 0.0001
Cell Periphery 0.0391 0.0378 0.0329 0.0531 0.074 0.0397 0.0727 0.0235 0.0283 0.0374 0.0919 0.0538 0.0301 0.0213 0.0313 0.0428 0.0423 0.0335
Cytoplasm 0.0027 0.0002 0.0001 0.0201 0.0002 0.0001 0.0002 0.0001 0 0.0001 0 0.0001 0.0008 0 0 0 0.0001 0
Cytoplasmic Foci 0.0058 0.0004 0.0003 0.0327 0.0003 0.0001 0.0863 0.0006 0.0004 0.0005 0.0002 0.0006 0.001 0.0001 0.0004 0.0007 0.0059 0.0002
Eisosomes 0.0001 0 0 0 0 0 0.0004 0 0.0001 0.0001 0 0 0.0001 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0012 0.0001 0.0001 0.0002 0.0001 0.0001 0 0 0 0.0001 0 0.0001 0.0011 0.0001 0 0 0.0001 0
Endosome 0.0097 0.0012 0.0026 0.0021 0.0019 0.0014 0.0013 0.0015 0.0011 0.0021 0.0007 0.0022 0.0062 0.0009 0.0015 0.0013 0.0012 0.0012
Golgi 0.0018 0.0001 0.0004 0.0001 0.0002 0.0001 0.001 0.0003 0.0002 0.0004 0.0001 0.001 0.001 0.0001 0.0006 0.0003 0.0002 0.0003
Lipid Particles 0.0221 0.0046 0.0177 0.007 0.005 0.0061 0.1147 0.0145 0.027 0.0245 0.0464 0.0508 0.0569 0.0216 0.036 0.0434 0.0313 0.0309
Mitochondria 0.0393 0.0071 0.0161 0.003 0.0047 0.0043 0.1453 0.0304 0.1688 0.1802 0.0182 0.0447 0.1174 0.0315 0.1462 0.0414 0.0254 0.0324
None 0.003 0 0 0.0187 0 0 0.0026 0.0001 0 0 0 0 0.0017 0 0 0 0.0005 0
Nuclear Periphery 0.0107 0.0016 0.0006 0.0011 0.0003 0.0106 0.0001 0.0013 0.0003 0.0009 0.0002 0.0019 0.004 0.0007 0.0007 0.0002 0.0004 0.0013
Nucleolus 0.084 0.0281 0.0299 0.0862 0.0343 0.0309 0.0562 0.1478 0.1252 0.1082 0.1144 0.0991 0.1068 0.0242 0.071 0.1051 0.1625 0.0581
Nucleus 0.0116 0.0095 0.0108 0.0163 0.0071 0.0093 0.0057 0.0113 0.007 0.0104 0.0153 0.0068 0.0092 0.0029 0.0058 0.0085 0.0114 0.0067
Peroxisomes 0.0015 0.0001 0.0002 0.0002 0.0002 0.0001 0.0049 0.001 0.0017 0.0021 0.0003 0.0009 0.001 0.0001 0.0012 0.0017 0.0009 0.0004
Punctate Nuclear 0.0044 0 0 0.0049 0 0 0.001 0 0 0 0 0 0.0004 0 0 0 0.0001 0
Vacuole 0.2914 0.3952 0.3264 0.3845 0.4715 0.3463 0.2451 0.183 0.1984 0.2341 0.2959 0.2737 0.1808 0.2898 0.2066 0.3296 0.2801 0.2511
Vacuole Periphery 0.4647 0.513 0.5611 0.3667 0.3979 0.5504 0.2597 0.5809 0.4399 0.3917 0.4154 0.4629 0.4771 0.6065 0.4963 0.4234 0.4353 0.5833

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.0913 76.6228 154.8729 212.2556 88.7394 70.3231 91.2046 124.6747 190.8582 98.1449
Translational Efficiency 1.1173 1.026 1.0388 1.1479 0.9347 0.9374 0.9439 1.0107 0.9571 0.8286

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘