Standard name | |
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Description | High affinity methionine permease; integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 4 | 2 | 1 | 2 | 5 | 4 | 3 | 4 | 0 | 0 | – | 0 | 1 | 1 | 2 | 1 | 6 |
Bud | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 4 | 5 | 7 | 9 | 3 | 3 | 5 | 0 | 0 | – | 1 | 0 | 1 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 14 | 0 | 11 | 21 | 22 | 8 | 29 | 77 | 111 | 131 | 112 | 250 | 17 | 17 | 33 | 8 | 0 | – | 2 | 0 | 0 | 0 | 0 | 2 |
Endoplasmic Reticulum | 0 | 0 | 1 | 4 | 1 | 2 | 4 | 4 | 5 | 6 | 2 | 4 | 0 | 0 | 0 | 2 | 1 | – | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 0 | 0 | 2 | 0 | 3 | 14 | 25 | 29 | 18 | 27 | 8 | 23 | 76 | 75 | 165 | 1 | 2 | – | 8 | 3 | 6 | 9 | 24 | 44 |
Golgi | 11 | 1 | 3 | 6 | 22 | 1 | 6 | 2 | 3 | 1 | 0 | 1 | 53 | 54 | 22 | 6 | 3 | – | 19 | 3 | 27 | 30 | 40 | 57 |
Mitochondria | 31 | 27 | 61 | 8 | 30 | 44 | 35 | 50 | 35 | 36 | 34 | 34 | 60 | 60 | 28 | 2 | 1 | – | 14 | 5 | 35 | 17 | 35 | 48 |
Nucleus | 40 | 15 | 33 | 63 | 50 | 86 | 173 | 132 | 90 | 112 | 30 | 54 | 48 | 48 | 58 | 13 | 5 | – | 18 | 6 | 13 | 9 | 6 | 5 |
Nuclear Periphery | 3 | 1 | 0 | 0 | 1 | 3 | 4 | 2 | 0 | 0 | 2 | 1 | 3 | 5 | 2 | 0 | 0 | – | 2 | 1 | 3 | 2 | 7 | 9 |
Nucleolus | 28 | 14 | 31 | 17 | 18 | 26 | 33 | 48 | 18 | 24 | 8 | 19 | 65 | 65 | 69 | 7 | 4 | – | 20 | 5 | 15 | 6 | 8 | 4 |
Peroxisomes | 0 | 0 | 0 | 0 | 2 | 3 | 4 | 8 | 5 | 0 | 3 | 2 | 21 | 23 | 28 | 0 | 0 | – | 0 | 0 | 1 | 0 | 2 | 3 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 6 | 20 | 19 | 14 | 15 | 11 | 18 | 62 | 61 | 123 | 0 | 0 | – | 0 | 0 | 1 | 1 | 2 | 2 |
Vac/Vac Membrane | 177 | 72 | 196 | 220 | 321 | 247 | 431 | 558 | 346 | 420 | 204 | 283 | 464 | 480 | 385 | 35 | 10 | – | 223 | 104 | 220 | 134 | 191 | 183 |
Unique Cell Count | 262 | 109 | 285 | 308 | 417 | 368 | 649 | 783 | 519 | 619 | 334 | 560 | 697 | 710 | 697 | 56 | 20 | 316 | 132 | 330 | 216 | 324 | 376 | |
Labelled Cell Count | 305 | 130 | 338 | 344 | 473 | 442 | 767 | 937 | 653 | 780 | 426 | 706 | 877 | 895 | 924 | 74 | 26 | 316 | 132 | 330 | 216 | 324 | 376 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 44.8 | 44.1 | 43.9 | 44.2 | 39.4 | 27.9 | 23.5 | 19.8 | 17.8 | 15.8 | 13.5 | 13.4 | 15.4 | 15.4 | 11.3 | 47.9 | 38.0 | – | 34.4 | 29.1 | 27.6 |
Std Deviation (1e-4) | 19.5 | 18.1 | 18.2 | 17.8 | 18.5 | 18.1 | 16.2 | 15.0 | 13.4 | 13.4 | 11.7 | 10.9 | 6.0 | 6.0 | 3.9 | 29.4 | 20.0 | – | 15.0 | 12.9 | 11.8 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.15 | -0.65 | -0.9 | -1.15 | -1.3 | -1.47 | -1.7 | -1.71 | -1.51 | -1.51 | -1.96 | 0.13 | -0.21 | – | -0.35 | -0.59 | -0.67 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cytoplasm | 1.6 | 0.9 | -1.3 | 0.4 | 3.1 | 6.6 | 6.6 | 9.2 | 12.1 | -1.2 | -1.3 | 0.6 | 0 | 0 | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Endosome | 0 | 0 | 2.5 | 2.6 | 2.6 | 2.4 | 2.9 | 0 | 2.8 | 5.4 | 5.3 | 8.7 | 0 | 0 | – |
Golgi | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 4.0 | 1.9 | 0 | 0 | – |
Mitochondria | -7.1 | -5.5 | -3.3 | -7.4 | -7.1 | -6.1 | -7.0 | -3.9 | -6.7 | -5.5 | -5.7 | -8.6 | -3.1 | 0 | – |
Nucleus | 2.9 | 0.2 | 3.9 | 5.1 | 2.1 | 2.2 | 2.5 | -1.1 | -0.9 | -2.4 | -2.5 | -1.6 | 2.3 | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleolus | -2.4 | -3.4 | -1.7 | -3.2 | -2.6 | -4.2 | -4.1 | -4.3 | -4.4 | -0.7 | -0.8 | -0.5 | 0.4 | 0 | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 3.1 | 3.4 | 0 | 0 | – |
SpindlePole | 0 | 0 | 0 | 3.0 | 2.7 | 0 | 0 | 3.1 | 3.1 | 5.2 | 5.1 | 7.6 | 0 | 0 | – |
Vacuole | 0.7 | 2.4 | -0.4 | -0.7 | 0.8 | -0.6 | -0.3 | -2.0 | -5.1 | -0.7 | -0.4 | -3.9 | -0.9 | -1.7 | – |