Standard name | |
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Description | High affinity methionine permease; integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 4 | 2 | 1 | 2 | 5 | 4 | 3 | 4 | 0 | 0 | – | 0 | 1 | 1 | 2 | 1 | 6 |
Bud | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 4 | 5 | 7 | 9 | 3 | 3 | 5 | 0 | 0 | – | 1 | 0 | 1 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 14 | 0 | 11 | 21 | 22 | 8 | 29 | 77 | 111 | 131 | 112 | 250 | 17 | 17 | 33 | 8 | 0 | – | 2 | 0 | 0 | 0 | 0 | 2 |
Endoplasmic Reticulum | 0 | 0 | 1 | 4 | 1 | 2 | 4 | 4 | 5 | 6 | 2 | 4 | 0 | 0 | 0 | 2 | 1 | – | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 0 | 0 | 2 | 0 | 3 | 14 | 25 | 29 | 18 | 27 | 8 | 23 | 76 | 75 | 165 | 1 | 2 | – | 8 | 3 | 6 | 9 | 24 | 44 |
Golgi | 11 | 1 | 3 | 6 | 22 | 1 | 6 | 2 | 3 | 1 | 0 | 1 | 53 | 54 | 22 | 6 | 3 | – | 19 | 3 | 27 | 30 | 40 | 57 |
Mitochondria | 31 | 27 | 61 | 8 | 30 | 44 | 35 | 50 | 35 | 36 | 34 | 34 | 60 | 60 | 28 | 2 | 1 | – | 14 | 5 | 35 | 17 | 35 | 48 |
Nucleus | 40 | 15 | 33 | 63 | 50 | 86 | 173 | 132 | 90 | 112 | 30 | 54 | 48 | 48 | 58 | 13 | 5 | – | 18 | 6 | 13 | 9 | 6 | 5 |
Nuclear Periphery | 3 | 1 | 0 | 0 | 1 | 3 | 4 | 2 | 0 | 0 | 2 | 1 | 3 | 5 | 2 | 0 | 0 | – | 2 | 1 | 3 | 2 | 7 | 9 |
Nucleolus | 28 | 14 | 31 | 17 | 18 | 26 | 33 | 48 | 18 | 24 | 8 | 19 | 65 | 65 | 69 | 7 | 4 | – | 20 | 5 | 15 | 6 | 8 | 4 |
Peroxisomes | 0 | 0 | 0 | 0 | 2 | 3 | 4 | 8 | 5 | 0 | 3 | 2 | 21 | 23 | 28 | 0 | 0 | – | 0 | 0 | 1 | 0 | 2 | 3 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 6 | 20 | 19 | 14 | 15 | 11 | 18 | 62 | 61 | 123 | 0 | 0 | – | 0 | 0 | 1 | 1 | 2 | 2 |
Vac/Vac Membrane | 177 | 72 | 196 | 220 | 321 | 247 | 431 | 558 | 346 | 420 | 204 | 283 | 464 | 480 | 385 | 35 | 10 | – | 223 | 104 | 220 | 134 | 191 | 183 |
Unique Cell Count | 262 | 109 | 285 | 308 | 417 | 368 | 649 | 783 | 519 | 619 | 334 | 560 | 697 | 710 | 697 | 56 | 20 | 316 | 132 | 330 | 216 | 324 | 376 | |
Labelled Cell Count | 305 | 130 | 338 | 344 | 473 | 442 | 767 | 937 | 653 | 780 | 426 | 706 | 877 | 895 | 924 | 74 | 26 | 316 | 132 | 330 | 216 | 324 | 376 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 44.8 | 44.1 | 43.9 | 44.2 | 39.4 | 27.9 | 23.5 | 19.8 | 17.8 | 15.8 | 13.5 | 13.4 | 15.4 | 15.4 | 11.3 | 47.9 | 38.0 | – | 34.4 | 29.1 | 27.6 |
Std Deviation (1e-4) | 19.5 | 18.1 | 18.2 | 17.8 | 18.5 | 18.1 | 16.2 | 15.0 | 13.4 | 13.4 | 11.7 | 10.9 | 6.0 | 6.0 | 3.9 | 29.4 | 20.0 | – | 15.0 | 12.9 | 11.8 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.15 | -0.65 | -0.9 | -1.15 | -1.3 | -1.47 | -1.7 | -1.71 | -1.51 | -1.51 | -1.96 | 0.13 | -0.21 | – | -0.35 | -0.59 | -0.67 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cytoplasm | 1.6 | 0.9 | -1.3 | 0.4 | 3.1 | 6.6 | 6.6 | 9.2 | 12.1 | -1.2 | -1.3 | 0.6 | 0 | 0 | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Endosome | 0 | 0 | 2.5 | 2.6 | 2.6 | 2.4 | 2.9 | 0 | 2.8 | 5.4 | 5.3 | 8.7 | 0 | 0 | – |
Golgi | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 4.0 | 1.9 | 0 | 0 | – |
Mitochondria | -7.1 | -5.5 | -3.3 | -7.4 | -7.1 | -6.1 | -7.0 | -3.9 | -6.7 | -5.5 | -5.7 | -8.6 | -3.1 | 0 | – |
Nucleus | 2.9 | 0.2 | 3.9 | 5.1 | 2.1 | 2.2 | 2.5 | -1.1 | -0.9 | -2.4 | -2.5 | -1.6 | 2.3 | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleolus | -2.4 | -3.4 | -1.7 | -3.2 | -2.6 | -4.2 | -4.1 | -4.3 | -4.4 | -0.7 | -0.8 | -0.5 | 0.4 | 0 | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 3.1 | 3.4 | 0 | 0 | – |
SpindlePole | 0 | 0 | 0 | 3.0 | 2.7 | 0 | 0 | 3.1 | 3.1 | 5.2 | 5.1 | 7.6 | 0 | 0 | – |
Vacuole | 0.7 | 2.4 | -0.4 | -0.7 | 0.8 | -0.6 | -0.3 | -2.0 | -5.1 | -0.7 | -0.4 | -3.9 | -0.9 | -1.7 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 37.9565 | 63.9243 | 58.6418 | 45.5666 | 56.4469 | 61.1304 | 43.2528 | 58.1299 | 57.6054 | 50.6654 | 53.5988 | 61.877 | 50.1976 | 75.6191 | 67.0112 | 62.4065 | 65.5332 | 67.9858 |
Actin | 0.0052 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0018 | 0 | 0.0001 | 0 | 0 | 0 |
Bud | 0.0002 | 0.0001 | 0.0001 | 0.0011 | 0.0005 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 |
Bud Neck | 0.0004 | 0.0004 | 0.0004 | 0.001 | 0.0004 | 0.0002 | 0.0004 | 0.0011 | 0.0003 | 0.0046 | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0013 | 0.0004 | 0.0003 | 0.0002 |
Bud Periphery | 0.0005 | 0.0003 | 0.0003 | 0.0007 | 0.0011 | 0.0003 | 0.0005 | 0.0005 | 0.0003 | 0.0011 | 0.0006 | 0.0005 | 0.0011 | 0.0002 | 0.0003 | 0.0005 | 0.0013 | 0.0002 |
Bud Site | 0.0008 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0 | 0.0006 | 0.0016 | 0.0005 | 0.0011 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0003 | 0.0003 | 0.0001 |
Cell Periphery | 0.0391 | 0.0378 | 0.0329 | 0.0531 | 0.074 | 0.0397 | 0.0727 | 0.0235 | 0.0283 | 0.0374 | 0.0919 | 0.0538 | 0.0301 | 0.0213 | 0.0313 | 0.0428 | 0.0423 | 0.0335 |
Cytoplasm | 0.0027 | 0.0002 | 0.0001 | 0.0201 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0008 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0058 | 0.0004 | 0.0003 | 0.0327 | 0.0003 | 0.0001 | 0.0863 | 0.0006 | 0.0004 | 0.0005 | 0.0002 | 0.0006 | 0.001 | 0.0001 | 0.0004 | 0.0007 | 0.0059 | 0.0002 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0012 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0011 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Endosome | 0.0097 | 0.0012 | 0.0026 | 0.0021 | 0.0019 | 0.0014 | 0.0013 | 0.0015 | 0.0011 | 0.0021 | 0.0007 | 0.0022 | 0.0062 | 0.0009 | 0.0015 | 0.0013 | 0.0012 | 0.0012 |
Golgi | 0.0018 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.001 | 0.001 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0.0003 |
Lipid Particles | 0.0221 | 0.0046 | 0.0177 | 0.007 | 0.005 | 0.0061 | 0.1147 | 0.0145 | 0.027 | 0.0245 | 0.0464 | 0.0508 | 0.0569 | 0.0216 | 0.036 | 0.0434 | 0.0313 | 0.0309 |
Mitochondria | 0.0393 | 0.0071 | 0.0161 | 0.003 | 0.0047 | 0.0043 | 0.1453 | 0.0304 | 0.1688 | 0.1802 | 0.0182 | 0.0447 | 0.1174 | 0.0315 | 0.1462 | 0.0414 | 0.0254 | 0.0324 |
None | 0.003 | 0 | 0 | 0.0187 | 0 | 0 | 0.0026 | 0.0001 | 0 | 0 | 0 | 0 | 0.0017 | 0 | 0 | 0 | 0.0005 | 0 |
Nuclear Periphery | 0.0107 | 0.0016 | 0.0006 | 0.0011 | 0.0003 | 0.0106 | 0.0001 | 0.0013 | 0.0003 | 0.0009 | 0.0002 | 0.0019 | 0.004 | 0.0007 | 0.0007 | 0.0002 | 0.0004 | 0.0013 |
Nucleolus | 0.084 | 0.0281 | 0.0299 | 0.0862 | 0.0343 | 0.0309 | 0.0562 | 0.1478 | 0.1252 | 0.1082 | 0.1144 | 0.0991 | 0.1068 | 0.0242 | 0.071 | 0.1051 | 0.1625 | 0.0581 |
Nucleus | 0.0116 | 0.0095 | 0.0108 | 0.0163 | 0.0071 | 0.0093 | 0.0057 | 0.0113 | 0.007 | 0.0104 | 0.0153 | 0.0068 | 0.0092 | 0.0029 | 0.0058 | 0.0085 | 0.0114 | 0.0067 |
Peroxisomes | 0.0015 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0049 | 0.001 | 0.0017 | 0.0021 | 0.0003 | 0.0009 | 0.001 | 0.0001 | 0.0012 | 0.0017 | 0.0009 | 0.0004 |
Punctate Nuclear | 0.0044 | 0 | 0 | 0.0049 | 0 | 0 | 0.001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 |
Vacuole | 0.2914 | 0.3952 | 0.3264 | 0.3845 | 0.4715 | 0.3463 | 0.2451 | 0.183 | 0.1984 | 0.2341 | 0.2959 | 0.2737 | 0.1808 | 0.2898 | 0.2066 | 0.3296 | 0.2801 | 0.2511 |
Vacuole Periphery | 0.4647 | 0.513 | 0.5611 | 0.3667 | 0.3979 | 0.5504 | 0.2597 | 0.5809 | 0.4399 | 0.3917 | 0.4154 | 0.4629 | 0.4771 | 0.6065 | 0.4963 | 0.4234 | 0.4353 | 0.5833 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 53.0913 | 76.6228 | 154.8729 | 212.2556 | 88.7394 | 70.3231 | 91.2046 | 124.6747 | 190.8582 | 98.1449 |
Translational Efficiency | 1.1173 | 1.026 | 1.0388 | 1.1479 | 0.9347 | 0.9374 | 0.9439 | 1.0107 | 0.9571 | 0.8286 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |