Standard name | |
---|---|
Human Ortholog | |
Description | Spt4p/5p (DSIF) transcription elongation factor complex subunit; the Spt4/5 complex binds to ssRNA in a sequence-specific manner, and along with RNAP I and II has multiple roles regulating transcriptional elongation, RNA processing, quality control, and transcription-coupled repair; localizes to kinetochores and heterochromatin, influencing chromosomal dynamics and silencing; required for transcription through long trinucleotide repeats in ORFs and non-protein coding regions |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 7 | 5 | 5 | 10 | 11 | 0 | 1 | 8 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 6 | 1 | 4 | 23 | 18 | 26 | 22 | 19 | 10 | 16 | 9 | 12 | 31 | 47 | 22 | 10 | 12 | 1 | 1 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 3 | 0 | 0 | 1 | 1 | 2 | 3 | 5 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 |
Mitochondria | 8 | 1 | 10 | 13 | 2 | 22 | 35 | 24 | 34 | 33 | 38 | 41 | 4 | 0 | 1 | 2 | 11 | 6 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleus | 407 | 237 | 120 | 253 | 343 | 310 | 420 | 522 | 294 | 368 | 311 | 364 | 388 | 520 | 487 | 143 | 74 | 101 | 406 | 237 | 116 | 98 | 267 | 309 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 4 | 2 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 184 | 123 | 67 | 133 | 103 | 192 | 261 | 437 | 249 | 335 | 231 | 360 | 208 | 192 | 222 | 38 | 13 | 29 | 54 | 42 | 22 | 40 | 118 | 97 |
Peroxisomes | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 9 | 17 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 2 | 3 | 2 | 0 | 3 | 4 | 7 | 5 | 5 | 2 | 1 | 1 | 1 | 4 | 2 | 4 | 3 | 2 | 6 | 5 | 3 | 3 | 26 | 41 |
Unique Cell Count | 457 | 272 | 141 | 297 | 403 | 415 | 579 | 781 | 445 | 562 | 439 | 569 | 451 | 604 | 622 | 190 | 96 | 131 | 483 | 293 | 147 | 150 | 417 | 460 |
Labelled Cell Count | 610 | 372 | 201 | 405 | 478 | 550 | 753 | 1024 | 610 | 757 | 610 | 795 | 619 | 763 | 796 | 212 | 113 | 153 | 483 | 293 | 147 | 150 | 417 | 460 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 23.7 | 27.7 | 14.3 | 18.2 | 19.7 | 15.0 | 15.7 | 15.1 | 14.5 | 14.1 | 14.4 | 13.5 | 26.4 | 28.5 | 26.0 | 36.8 | 34.9 | 34.4 | 22.3 | 27.3 | 28.1 |
Std Deviation (1e-4) | 8.4 | 7.3 | 5.1 | 6.2 | 9.3 | 6.3 | 7.2 | 6.5 | 7.8 | 6.0 | 6.3 | 5.5 | 8.3 | 9.7 | 10.3 | 16.7 | 14.3 | 14.0 | 6.8 | 8.6 | 8.1 |
Intensity Change (Log2) | – | – | – | 0.35 | 0.46 | 0.07 | 0.13 | 0.08 | 0.02 | -0.02 | 0.01 | -0.08 | 0.89 | 0.99 | 0.86 | 1.37 | 1.29 | 1.27 | 0.64 | 0.93 | 0.97 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.5 | 0 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 3.0 | 3.8 | 0 | 3.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.2 | 0 | -0.8 | -0.5 | -2.3 | 0.2 | -0.5 | 0.6 | 0 | 0 | 0 | 0 | -2.9 | 1.2 | -0.9 |
Nucleus | 0 | 0 | -2.6 | -3.1 | -4.3 | -4.3 | -4.5 | -3.4 | -4.8 | 0.3 | 0.3 | -1.8 | -2.2 | -1.6 | -1.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.5 | -4.8 | -0.3 | -0.5 | 1.9 | 1.8 | 2.6 | 1.1 | 3.4 | -0.3 | -3.5 | -2.6 | -5.3 | -5.4 | -4.4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 18.5259 | 17.988 | 15.7437 | 17.0001 | 15.4356 | 16.5184 | 18.2571 | 17.8966 | 16.1489 | 15.2759 | 14.5349 | 15.6195 | 21.3359 | 21.5188 | 19.9507 | 21.2425 | 17.8328 | 19.157 |
Actin | 0.0139 | 0.0002 | 0.0106 | 0.0309 | 0.0028 | 0.0056 | 0.0139 | 0 | 0.0098 | 0.003 | 0.0052 | 0.0038 | 0.0428 | 0 | 0.0111 | 0 | 0 | 0 |
Bud | 0.0002 | 0.003 | 0.0005 | 0 | 0.0004 | 0.0003 | 0.0004 | 0 | 0.0005 | 0.0012 | 0.0004 | 0.0002 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.005 | 0.0008 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0006 | 0 | 0.0032 | 0 | 0.0001 | 0.0001 |
Bud Periphery | 0.0003 | 0.0007 | 0.001 | 0 | 0.0015 | 0.0003 | 0.0004 | 0 | 0.0006 | 0.0025 | 0.0005 | 0.0003 | 0.0005 | 0 | 0.0003 | 0 | 0.0002 | 0.0001 |
Bud Site | 0.0005 | 0.0018 | 0.0029 | 0.0001 | 0.0011 | 0.0003 | 0.0015 | 0 | 0.0019 | 0.0004 | 0.001 | 0.0002 | 0.0007 | 0 | 0.0051 | 0 | 0.0002 | 0 |
Cell Periphery | 0.0004 | 0.0001 | 0.0008 | 0 | 0.0034 | 0.0005 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0005 | 0 | 0.0002 | 0.0001 |
Cytoplasm | 0.0026 | 0.0033 | 0.0047 | 0.0001 | 0.0028 | 0.0089 | 0.0066 | 0 | 0.006 | 0.0017 | 0.0017 | 0.0074 | 0.003 | 0 | 0.0004 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0075 | 0.0007 | 0.0119 | 0.0035 | 0.0126 | 0.0091 | 0.0148 | 0 | 0.0047 | 0.0006 | 0.053 | 0.0012 | 0.0026 | 0 | 0.002 | 0 | 0.0002 | 0.0005 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0011 | 0 | 0.0034 | 0 | 0.0012 | 0.0011 | 0.0026 | 0 | 0.0007 | 0.0053 | 0.0015 | 0.0015 | 0.0017 | 0 | 0.0002 | 0 | 0 | 0 |
Endosome | 0.0036 | 0 | 0.0076 | 0.0006 | 0.0126 | 0.0045 | 0.0143 | 0 | 0.0035 | 0.0101 | 0.006 | 0.0151 | 0.0104 | 0 | 0.0011 | 0 | 0.0002 | 0.0001 |
Golgi | 0.002 | 0 | 0.0028 | 0.002 | 0.0019 | 0.001 | 0.0036 | 0 | 0.0036 | 0.0025 | 0.0027 | 0.003 | 0.0024 | 0 | 0.0009 | 0 | 0 | 0 |
Lipid Particles | 0.0079 | 0 | 0.0068 | 0.0064 | 0.0103 | 0.0037 | 0.0081 | 0 | 0.0031 | 0.0002 | 0.0222 | 0.0037 | 0.0031 | 0 | 0.0016 | 0 | 0.0006 | 0.0015 |
Mitochondria | 0.0037 | 0.0003 | 0.0049 | 0.0003 | 0.0068 | 0.0013 | 0.0049 | 0.0002 | 0.0072 | 0.05 | 0.0177 | 0.0023 | 0.015 | 0.0002 | 0.001 | 0.0003 | 0.0026 | 0.0041 |
None | 0.0013 | 0.0002 | 0.0044 | 0.0003 | 0.0061 | 0.0016 | 0.004 | 0 | 0.0008 | 0.0004 | 0.0048 | 0.0018 | 0.0121 | 0 | 0.0004 | 0 | 0.0002 | 0 |
Nuclear Periphery | 0.0036 | 0.0003 | 0.0039 | 0.0001 | 0.0174 | 0.01 | 0.0217 | 0.0001 | 0.0037 | 0.0364 | 0.0188 | 0.02 | 0.0184 | 0.0006 | 0.0006 | 0 | 0.0006 | 0.0001 |
Nucleolus | 0.1136 | 0.0701 | 0.0659 | 0.0262 | 0.4611 | 0.1917 | 0.0796 | 0.0301 | 0.051 | 0.0158 | 0.4777 | 0.1876 | 0.0765 | 0.0272 | 0.0588 | 0.068 | 0.369 | 0.1924 |
Nucleus | 0.8189 | 0.9185 | 0.8523 | 0.9113 | 0.42 | 0.7534 | 0.7955 | 0.9694 | 0.8914 | 0.8652 | 0.3462 | 0.7404 | 0.7983 | 0.9718 | 0.9069 | 0.9315 | 0.6221 | 0.7982 |
Peroxisomes | 0.0093 | 0 | 0.0054 | 0.017 | 0.002 | 0.0003 | 0.0054 | 0 | 0.0054 | 0.0002 | 0.0021 | 0.0003 | 0.0028 | 0 | 0.0015 | 0 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0045 | 0.0003 | 0.0067 | 0.001 | 0.0114 | 0.0034 | 0.0195 | 0 | 0.0048 | 0.0004 | 0.0293 | 0.0046 | 0.004 | 0 | 0.0022 | 0 | 0.0022 | 0.0001 |
Vacuole | 0.0022 | 0.0003 | 0.0016 | 0 | 0.014 | 0.001 | 0.0014 | 0 | 0.0005 | 0.0015 | 0.0025 | 0.0029 | 0.002 | 0 | 0.0012 | 0 | 0.0006 | 0.0008 |
Vacuole Periphery | 0.0025 | 0.0001 | 0.0012 | 0 | 0.0053 | 0.001 | 0.0015 | 0 | 0.0006 | 0.0023 | 0.0053 | 0.0031 | 0.002 | 0 | 0.0005 | 0 | 0.0008 | 0.0016 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 49.9924 | 54.2667 | 75.799 | 47.6407 | 71.7621 | 49.9648 | 62.131 | 46.8357 | 66.5208 | 58.4726 |
Translational Efficiency | 2.7707 | 2.1847 | 1.6088 | 2.7912 | 1.6454 | 2.7933 | 2.0373 | 3.377 | 1.9621 | 2.3697 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
251 | 1699 | 1726 | 1006 | 2529 | 1590 | 1152 | 1378 | 2780 | 3289 | 2878 | 2384 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1493.77 | 1859.91 | 2212.77 | 2659.85 | 1672.41 | 1813.31 | 2461.33 | 2495.48 | 1656.28 | 1837.38 | 2312.26 | 2564.84 |
Standard Deviation | 326.15 | 469.06 | 499.60 | 746.92 | 377.42 | 401.15 | 571.46 | 665.76 | 376.57 | 438.17 | 543.36 | 705.84 |
Intensity Change Log 2 | — | 0.316275 | 0.566895 | 0.832387 | — | 0.116697 | 0.557510 | 0.577389 | — | 0.214302 | 0.561945 | 0.703322 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000411 | 0.000468 | 0.000638 | 0.000679 | 0.000501 | 0.000567 | 0.000546 | 0.000625 | 0.000493 | 0.000516 | 0.000601 | 0.000648 |
Bud Neck | 0.001462 | 0.001611 | 0.004631 | 0.004171 | 0.002795 | 0.002638 | 0.003120 | 0.006386 | 0.002674 | 0.002108 | 0.004026 | 0.005451 |
Bud Site | 0.000145 | 0.000210 | 0.000528 | 0.000894 | 0.000322 | 0.000520 | 0.000410 | 0.001650 | 0.000306 | 0.000360 | 0.000481 | 0.001331 |
Cell Periphery | 0.000006 | 0.000030 | 0.000019 | 0.000109 | 0.000027 | 0.000098 | 0.000034 | 0.000210 | 0.000025 | 0.000063 | 0.000025 | 0.000167 |
Cytoplasm | 0.000005 | 0.000202 | 0.000014 | 0.000463 | 0.000015 | 0.000105 | 0.000019 | 0.000756 | 0.000014 | 0.000155 | 0.000016 | 0.000632 |
Cytoplasmic Foci | 0.000056 | 0.000242 | 0.000231 | 0.000909 | 0.000135 | 0.000777 | 0.000310 | 0.003245 | 0.000128 | 0.000501 | 0.000263 | 0.002260 |
Eisosomes | 0.000105 | 0.000247 | 0.000235 | 0.000080 | 0.000337 | 0.000418 | 0.000212 | 0.000106 | 0.000316 | 0.000330 | 0.000226 | 0.000095 |
Endoplasmic Reticulum | 0.000149 | 0.000145 | 0.000121 | 0.000150 | 0.000219 | 0.000221 | 0.000101 | 0.000299 | 0.000212 | 0.000182 | 0.000113 | 0.000236 |
Endosome | 0.000002 | 0.000033 | 0.000002 | 0.000061 | 0.000009 | 0.000049 | 0.000003 | 0.001207 | 0.000009 | 0.000041 | 0.000003 | 0.000724 |
Golgi | 0.000014 | 0.000053 | 0.000022 | 0.000230 | 0.000014 | 0.000062 | 0.000013 | 0.000923 | 0.000014 | 0.000057 | 0.000018 | 0.000631 |
Lipid Particles | 0.000001 | 0.000021 | 0.000002 | 0.000021 | 0.000008 | 0.000043 | 0.000001 | 0.000159 | 0.000007 | 0.000031 | 0.000002 | 0.000101 |
Mitochondria | 0.000710 | 0.000705 | 0.001352 | 0.002182 | 0.000688 | 0.001433 | 0.001075 | 0.002059 | 0.000690 | 0.001057 | 0.001241 | 0.002111 |
Mitotic Spindle | 0.000006 | 0.000250 | 0.000051 | 0.000947 | 0.000013 | 0.002233 | 0.000006 | 0.002924 | 0.000013 | 0.001209 | 0.000033 | 0.002090 |
None | 0.000422 | 0.000553 | 0.000415 | 0.000944 | 0.000325 | 0.000753 | 0.000408 | 0.001753 | 0.000334 | 0.000650 | 0.000412 | 0.001411 |
Nuclear Periphery | 0.000006 | 0.000015 | 0.000017 | 0.000164 | 0.000013 | 0.000037 | 0.000011 | 0.000573 | 0.000013 | 0.000026 | 0.000015 | 0.000400 |
Nuclear Periphery Foci | 0.000001 | 0.000091 | 0.000001 | 0.000059 | 0.000026 | 0.000175 | 0.000001 | 0.000787 | 0.000024 | 0.000132 | 0.000001 | 0.000480 |
Nucleolus | 0.091685 | 0.090927 | 0.061289 | 0.034682 | 0.082846 | 0.115538 | 0.037716 | 0.020955 | 0.083644 | 0.102825 | 0.051853 | 0.026748 |
Nucleus | 0.895023* | 0.895279* | 0.919906* | 0.949674* | 0.907292* | 0.863042* | 0.949470* | 0.950541* | 0.906184* | 0.879695* | 0.931740* | 0.950175* |
Peroxisomes | 0.009583 | 0.007920 | 0.010207 | 0.002800 | 0.004037 | 0.010576 | 0.006229 | 0.002750 | 0.004538 | 0.009204 | 0.008615 | 0.002771 |
Vacuole | 0.000076 | 0.000403 | 0.000115 | 0.000475 | 0.000166 | 0.000383 | 0.000138 | 0.001150 | 0.000158 | 0.000393 | 0.000125 | 0.000865 |
Vacuole Periphery | 0.000130 | 0.000593 | 0.000204 | 0.000306 | 0.000211 | 0.000331 | 0.000177 | 0.000942 | 0.000204 | 0.000466 | 0.000193 | 0.000673 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.21 | -9.21 | -5.93 | -4.89 | -1.11 | -3.03 | -2.69 | -4.94 | -2.24 | -3.28 | -1.71 | -8.31 | -6.77 | -5.32 | -2.52 |
Bud Neck | -1.13 | -17.75 | -9.56 | -9.59 | 0.59 | 0.25 | -1.76 | -7.85 | -7.44 | -7.19 | 3.24 | -9.14 | -9.45 | -10.94 | -5.47 |
Bud Site | -3.22 | -15.10 | -2.63 | -2.43 | -1.40 | -2.01 | -0.70 | -3.99 | -3.33 | -3.68 | -1.03 | -2.87 | -4.52 | -4.24 | -3.88 |
Cell Periphery | -2.19 | -9.76 | -2.79 | -2.08 | -2.46 | -1.54 | -0.38 | -3.79 | -1.80 | -3.58 | -1.60 | 0.21 | -4.42 | -2.79 | -4.46 |
Cytoplasm | -1.48 | -4.08 | -3.08 | -1.09 | -3.03 | -3.12 | -0.18 | -3.08 | -2.75 | -3.07 | -1.92 | 0.08 | -3.96 | -2.79 | -3.97 |
Cytoplasmic Foci | -2.56 | -9.69 | -2.64 | -2.11 | -2.15 | -2.01 | -0.78 | -3.78 | -2.78 | -3.63 | -1.94 | -0.81 | -4.26 | -3.44 | -4.15 |
Eisosomes | -10.99 | -12.18 | 2.64 | 15.08 | 18.34 | -0.64 | 7.02 | 14.58 | 2.47 | 8.71 | -0.22 | 6.10 | 15.97 | 3.82 | 17.88 |
Endoplasmic Reticulum | -0.05 | 2.05 | -0.50 | -0.16 | -2.83 | -0.51 | 14.32 | -2.44 | -1.78 | -5.18 | 0.88 | 14.46 | -1.60 | -1.82 | -5.48 |
Endosome | -1.52 | -0.98 | -1.42 | -0.65 | -1.42 | -1.93 | 1.01 | -2.56 | -2.45 | -2.58 | -1.96 | 1.05 | -2.64 | -2.50 | -2.68 |
Golgi | -1.99 | -4.64 | -1.34 | -1.10 | -1.29 | -2.40 | 0.72 | -2.42 | -2.24 | -2.42 | -3.11 | -2.24 | -2.72 | -2.49 | -2.70 |
Lipid Particles | -1.38 | -1.99 | -1.58 | -0.04 | -1.55 | -1.58 | 0.97 | -2.48 | -1.62 | -2.70 | -1.58 | 0.93 | -2.55 | -1.69 | -2.92 |
Mitochondria | 0.04 | -9.85 | -6.42 | -6.51 | -3.67 | -1.50 | -7.55 | -7.18 | -1.26 | -5.11 | -1.53 | -12.99 | -9.66 | -3.93 | -5.91 |
Mitotic Spindle | -1.52 | -1.96 | -1.90 | -1.42 | -1.82 | -2.56 | 1.48 | -3.84 | -0.64 | -3.85 | -2.78 | -1.46 | -4.28 | -1.45 | -4.23 |
None | -1.13 | 0.03 | -4.85 | -2.53 | -4.96 | -3.64 | -2.46 | -3.19 | -2.13 | -3.00 | -3.80 | -4.06 | -4.14 | -2.76 | -3.82 |
Nuclear Periphery | -2.31 | -5.10 | -2.62 | -2.47 | -2.45 | -2.92 | 0.95 | -2.14 | -2.03 | -2.15 | -2.84 | -0.73 | -2.56 | -2.46 | -2.55 |
Nuclear Periphery Foci | -2.32 | 0.04 | -1.68 | 0.57 | -1.68 | -1.88 | 1.06 | -2.00 | -1.45 | -2.12 | -2.07 | 1.05 | -2.05 | -1.45 | -2.23 |
Nucleolus | 0.08 | 3.64 | 6.86 | 12.24 | 6.72 | -7.11 | 12.20 | 18.60 | 22.03* | 4.71 | -5.38 | 9.93 | 18.55 | 24.50 | 9.58 |
Nucleus | -0.00 | -2.62 | -5.72 | -9.78 | -5.97 | 8.64 | -10.29 | -8.24 | -14.35 | 0.56 | 6.82 | -7.18 | -10.78 | -16.88 | -4.57 |
Peroxisomes | 1.12 | -0.63 | 4.95 | 8.19 | 9.71 | -9.68 | -3.50 | 2.48 | 10.29 | 4.69 | -9.63 | -7.63 | 4.77 | 12.87 | 10.47 |
Vacuole | -1.48 | -5.76 | -1.97 | -0.38 | -1.79 | -1.57 | 0.79 | -2.27 | -1.68 | -2.37 | -1.69 | 1.05 | -2.67 | -1.65 | -2.87 |
Vacuole Periphery | -1.49 | -7.27 | -4.55 | 0.88 | -2.75 | -1.77 | 1.44 | -1.83 | -1.48 | -1.92 | -1.61 | 0.62 | -2.07 | -0.68 | -2.12 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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