Standard name
Description Spt4p/5p (DSIF) transcription elongation factor complex subunit; the Spt4/5 complex binds to ssRNA in a sequence-specific manner, and along with RNAP I and II has multiple roles regulating transcriptional elongation, RNA processing, quality control, and transcription-coupled repair; localizes to kinetochores and heterochromatin, influencing chromosomal dynamics and silencing; required for transcription through long trinucleotide repeats in ORFs and non-protein coding regions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0.05 0.08 0.12 0.1 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0 0 0.05 0.06 0 0.08 0.06 0.09 0.07 0 0 0 0 0.11 0.05 0 0 0 0 0 0
Nucleus 0.89 0.87 0.85 0.85 0.85 0.75 0.73 0.67 0.66 0.65 0.71 0.64 0.86 0.86 0.78 0.75 0.77 0.77 0.84 0.81 0.79 0.66 0.64 0.67
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.4 0.45 0.48 0.45 0.26 0.46 0.45 0.56 0.56 0.6 0.53 0.63 0.46 0.32 0.36 0.2 0.14 0.22 0.11 0.14 0.15 0.27 0.28 0.21
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 2 0 0 0 2 0 0 0 0 0 0 3 3 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 2 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 1 0 1 2 7 5 5 10 11 0 1 8 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 1 0 0 0 0 0 0
Cytoplasm 9 6 1 4 23 18 26 22 19 10 16 9 12 31 47 22 10 12 1 1 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 2 1 0 0 0 0 0
Endosome 0 1 0 0 1 0 2 3 0 0 1 1 2 3 5 0 0 0 2 1 1 1 0 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 1
Mitochondria 8 1 10 13 2 22 35 24 34 33 38 41 4 0 1 2 11 6 0 0 0 0 1 1
Nucleus 407 237 120 253 343 310 420 522 294 368 311 364 388 520 487 143 74 101 406 237 116 98 267 309
Nuclear Periphery 0 0 0 0 2 3 0 0 4 2 1 4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 184 123 67 133 103 192 261 437 249 335 231 360 208 192 222 38 13 29 54 42 22 40 118 97
Peroxisomes 0 1 0 1 1 0 0 0 0 0 0 0 0 3 6 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 4 9 17 0 0 0 2 0 0 0 1 0
Vac/Vac Membrane 2 3 2 0 3 4 7 5 5 2 1 1 1 4 2 4 3 2 6 5 3 3 26 41
Unique Cell Count 457 272 141 297 403 415 579 781 445 562 439 569 451 604 622 190 96 131 483 293 147 150 417 460
Labelled Cell Count 610 372 201 405 478 550 753 1024 610 757 610 795 619 763 796 212 113 153 483 293 147 150 417 460


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.7 27.7 14.3 18.2 19.7 15.0 15.7 15.1 14.5 14.1 14.4 13.5 26.4 28.5 26.0 36.8 34.9 34.4 22.3 27.3 28.1
Std Deviation (1e-4) 8.4 7.3 5.1 6.2 9.3 6.3 7.2 6.5 7.8 6.0 6.3 5.5 8.3 9.7 10.3 16.7 14.3 14.0 6.8 8.6 8.1
Intensity Change (Log2) – – – 0.35 0.46 0.07 0.13 0.08 0.02 -0.02 0.01 -0.08 0.89 0.99 0.86 1.37 1.29 1.27 0.64 0.93 0.97


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.5 0 2.1 0 0 0 0 0 0 2.3 3.0 3.8 0 3.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.2 0 -0.8 -0.5 -2.3 0.2 -0.5 0.6 0 0 0 0 -2.9 1.2 -0.9
Nucleus 0 0 -2.6 -3.1 -4.3 -4.3 -4.5 -3.4 -4.8 0.3 0.3 -1.8 -2.2 -1.6 -1.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.5 -4.8 -0.3 -0.5 1.9 1.8 2.6 1.1 3.4 -0.3 -3.5 -2.6 -5.3 -5.4 -4.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.5259 17.988 15.7437 17.0001 15.4356 16.5184 18.2571 17.8966 16.1489 15.2759 14.5349 15.6195 21.3359 21.5188 19.9507 21.2425 17.8328 19.157
Actin 0.0139 0.0002 0.0106 0.0309 0.0028 0.0056 0.0139 0 0.0098 0.003 0.0052 0.0038 0.0428 0 0.0111 0 0 0
Bud 0.0002 0.003 0.0005 0 0.0004 0.0003 0.0004 0 0.0005 0.0012 0.0004 0.0002 0.0003 0 0.0002 0 0.0001 0.0001
Bud Neck 0.0003 0.0001 0.0004 0.0002 0.005 0.0008 0.0002 0 0.0001 0.0001 0.0002 0.0005 0.0006 0 0.0032 0 0.0001 0.0001
Bud Periphery 0.0003 0.0007 0.001 0 0.0015 0.0003 0.0004 0 0.0006 0.0025 0.0005 0.0003 0.0005 0 0.0003 0 0.0002 0.0001
Bud Site 0.0005 0.0018 0.0029 0.0001 0.0011 0.0003 0.0015 0 0.0019 0.0004 0.001 0.0002 0.0007 0 0.0051 0 0.0002 0
Cell Periphery 0.0004 0.0001 0.0008 0 0.0034 0.0005 0.0002 0 0.0001 0.0001 0.0009 0.0001 0.0001 0 0.0005 0 0.0002 0.0001
Cytoplasm 0.0026 0.0033 0.0047 0.0001 0.0028 0.0089 0.0066 0 0.006 0.0017 0.0017 0.0074 0.003 0 0.0004 0 0 0
Cytoplasmic Foci 0.0075 0.0007 0.0119 0.0035 0.0126 0.0091 0.0148 0 0.0047 0.0006 0.053 0.0012 0.0026 0 0.002 0 0.0002 0.0005
Eisosomes 0.0001 0 0.0002 0.0001 0.0003 0.0001 0.0001 0 0 0 0.0002 0 0.0004 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0011 0 0.0034 0 0.0012 0.0011 0.0026 0 0.0007 0.0053 0.0015 0.0015 0.0017 0 0.0002 0 0 0
Endosome 0.0036 0 0.0076 0.0006 0.0126 0.0045 0.0143 0 0.0035 0.0101 0.006 0.0151 0.0104 0 0.0011 0 0.0002 0.0001
Golgi 0.002 0 0.0028 0.002 0.0019 0.001 0.0036 0 0.0036 0.0025 0.0027 0.003 0.0024 0 0.0009 0 0 0
Lipid Particles 0.0079 0 0.0068 0.0064 0.0103 0.0037 0.0081 0 0.0031 0.0002 0.0222 0.0037 0.0031 0 0.0016 0 0.0006 0.0015
Mitochondria 0.0037 0.0003 0.0049 0.0003 0.0068 0.0013 0.0049 0.0002 0.0072 0.05 0.0177 0.0023 0.015 0.0002 0.001 0.0003 0.0026 0.0041
None 0.0013 0.0002 0.0044 0.0003 0.0061 0.0016 0.004 0 0.0008 0.0004 0.0048 0.0018 0.0121 0 0.0004 0 0.0002 0
Nuclear Periphery 0.0036 0.0003 0.0039 0.0001 0.0174 0.01 0.0217 0.0001 0.0037 0.0364 0.0188 0.02 0.0184 0.0006 0.0006 0 0.0006 0.0001
Nucleolus 0.1136 0.0701 0.0659 0.0262 0.4611 0.1917 0.0796 0.0301 0.051 0.0158 0.4777 0.1876 0.0765 0.0272 0.0588 0.068 0.369 0.1924
Nucleus 0.8189 0.9185 0.8523 0.9113 0.42 0.7534 0.7955 0.9694 0.8914 0.8652 0.3462 0.7404 0.7983 0.9718 0.9069 0.9315 0.6221 0.7982
Peroxisomes 0.0093 0 0.0054 0.017 0.002 0.0003 0.0054 0 0.0054 0.0002 0.0021 0.0003 0.0028 0 0.0015 0 0.0001 0.0001
Punctate Nuclear 0.0045 0.0003 0.0067 0.001 0.0114 0.0034 0.0195 0 0.0048 0.0004 0.0293 0.0046 0.004 0 0.0022 0 0.0022 0.0001
Vacuole 0.0022 0.0003 0.0016 0 0.014 0.001 0.0014 0 0.0005 0.0015 0.0025 0.0029 0.002 0 0.0012 0 0.0006 0.0008
Vacuole Periphery 0.0025 0.0001 0.0012 0 0.0053 0.001 0.0015 0 0.0006 0.0023 0.0053 0.0031 0.002 0 0.0005 0 0.0008 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.9924 54.2667 75.799 47.6407 71.7621 49.9648 62.131 46.8357 66.5208 58.4726
Translational Efficiency 2.7707 2.1847 1.6088 2.7912 1.6454 2.7933 2.0373 3.377 1.9621 2.3697

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘