Standard name
Description Trans-golgi network aminophospholipid translocase (flippase); maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation, endocytosis, protein trafficking between the Golgi and endosomal system and the cellular response to mating pheromone; autoinhibited by its C-terminal tail; localizes to the trans-Golgi network; mutations in human homolog ATP8B1 result in liver disease

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.15 0.23 0.11 0.05 0 0 0 0 0 0 0 0 0.14 0.13 0.07 0.29 0.1 0.12 0.14 0.14 0.14 0.1
Bud 0 0 0 0 0 0.05 0 0 0.06 0.06 0.09 0.06 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.06 0.05 0 0 0 0 0 0 0 0.06 0.06 0 0.29 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0.14 0.06 0.05 0 0 0 0 0 0 0 0 0.13 0.11 0 0 0 0 0 0 0 0
Endosome 0.08 0 0.09 0.1 0.17 0 0.07 0.05 0 0 0 0 0.05 0.09 0.08 0.12 0.15 0.14 0.12 0.11 0.08 0.08
Golgi 0.7 0.55 0.59 0.26 0.15 0 0 0 0 0 0 0 0.64 0.68 0.69 0.35 0.65 0.61 0.62 0.63 0.67 0.68
Mitochondria 0.41 0.53 0.54 0.75 0.71 0.9 0.86 0.84 0.94 0.91 0.94 0.92 0.27 0.17 0.16 0.18 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0.06 0.07 0.05 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0.05 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 68 62 46 13 4 6 7 19 10 4 10 10 16 21 12 5 52 40 67 43 50 41
Bud 2 9 5 6 13 23 22 30 26 31 50 32 1 2 2 0 4 4 1 1 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 5 7 7 6 2 14 12 0 0 0 0
Cell Periphery 1 2 3 1 3 1 7 2 1 1 3 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 16 15 10 15 15 14 14 18 8 15 10 8 7 10 7 5 2 3 1 1 2 0
Endoplasmic Reticulum 28 38 23 12 5 7 3 11 5 6 14 6 15 18 4 0 7 8 2 3 6 11
Endosome 36 5 37 26 52 16 47 40 4 6 6 12 6 14 13 2 78 44 60 33 28 34
Golgi 319 147 242 69 45 17 12 26 10 7 4 24 72 111 118 6 343 196 306 199 251 289
Mitochondria 185 142 222 196 220 422 589 615 401 452 530 511 30 28 27 3 7 8 11 11 13 14
Nucleus 2 2 0 0 0 0 0 3 4 3 0 3 1 0 1 0 0 0 0 0 0 0
Nuclear Periphery 1 1 4 4 2 4 6 10 5 7 10 12 0 0 0 0 0 1 1 0 0 1
Nucleolus 8 0 7 7 8 25 39 52 23 27 27 29 1 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 15 12 14 11 51 36 8 13 22 14 0 0 2 0 10 2 36 14 10 17
SpindlePole 1 0 5 2 3 8 11 12 2 3 5 5 0 2 3 0 3 1 1 1 2 1
Vac/Vac Membrane 26 8 9 4 8 6 22 12 3 14 3 10 4 15 16 0 12 10 4 2 3 3
Unique Cell Count 454 266 411 263 309 467 684 731 426 498 565 556 113 163 170 17 527 325 498 315 375 423
Labelled Cell Count 693 431 628 367 393 565 837 893 516 591 708 688 153 222 206 21 527 325 498 315 375 423


Early Golgi, Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.6 13.0 10.5 9.8 9.2 8.2 8.0 8.0 7.6 7.1 7.0 7.4 10.7 11.5 11.8 9.8 11.7 12.2 12.7
Std Deviation (1e-4) 1.8 2.2 1.7 1.7 1.8 1.7 1.8 1.8 1.7 1.5 1.7 1.8 2.0 2.0 2.2 2.2 1.8 1.8 1.8
Intensity Change (Log2) -0.1 -0.2 -0.37 -0.4 -0.4 -0.48 -0.57 -0.59 -0.51 0.02 0.12 0.17 -0.1 0.15 0.21 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.8 -5.2 -6.2 -7.6 -6.0 -5.1 -6.8 -6.2 -6.2 0.9 0.6 -1.5 0
Bud 0 2.5 3.1 2.1 2.7 3.7 3.9 5.1 3.6 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 3.2 3.0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.2 1.8 0.5 -0.4 0 -0.6 0.5 -0.7 -1.1 0 2.2 0 0
Endoplasmic Reticulum -0.6 -2.7 -3.3 -5.4 -3.9 -3.6 -3.7 -2.5 -4.1 2.8 2.3 -1.7 0
Endosome 0.4 3.2 -3.5 -1.3 -2.3 -5.4 -5.5 -6.0 -4.8 -1.3 -0.2 -0.5 0
Golgi -8.3 -12.0 -17.9 -21.7 -21.2 -17.8 -19.3 -20.7 -18.8 0.9 2.0 2.4 -1.9
Mitochondria 5.4 4.7 12.2 11.7 11.0 13.3 12.6 14.6 13.6 -5.2 -8.0 -8.5 -2.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0.6 0 0 1.0 1.4 0 0 0 0
Nucleolus 0.9 0.8 2.9 3.2 4.0 2.9 2.9 2.6 2.9 0 0 0 0
Peroxisomes 0.6 0.6 -1.1 2.6 1.0 -1.6 -0.9 0.2 -1.0 0 0 0 0
SpindlePole 0 0 0.6 0.5 0.6 0 0 0 0 0 0 0 0
Vacuole -0.6 0.3 -1.0 1.0 -0.7 -1.8 0.6 -2.3 -0.4 0 3.8 3.9 0