Standard name
Description Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; relocalizes to the cytosol in response to hypoxia; homolog of human Sm D1; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0 0 0 0 0 0.07 0.12 0.15 0.05 0.13 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0.07 0.06 0.08 0.08 0.17 0.13 0.15 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.92 0.9 0.9 0.8 0.83 0.73 0.74 0.75 0.74 0.74 0.88 0.82 0.77 0.87 0.78 0.88 0.94 0.91 0.88 0.8 0.72 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0.09 0.15 0.11 0.16 0.25 0.34 0.39 0.39 0.38 0.34 0.06 0.06 0.06 0.06 0.05 0 0 0 0 0.07 0.12 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.12 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 1 1 1 0 3 2 2 0 2 1 0 0 0 3 1 1 2 1 6
Bud Neck 0 0 0 0 0 0 3 0 3 0 0 0 0 1 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 1 2 5 15 6 20 12 23 0 4 0 2 2 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 10 8 12 17 30 10 21 6 7 7 8 25 66 85 15 25 21 0 2 2 0 2 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 1 3 0 0 0 0 0 1
Endosome 1 0 1 0 3 0 0 0 0 0 0 2 0 4 3 1 1 4 0 5 2 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 2 0 1 1 0 1
Mitochondria 13 1 36 29 28 60 70 69 95 67 80 1 3 0 8 8 5 4 2 0 0 4 5
Nucleus 518 209 494 354 408 591 631 299 538 323 369 306 456 450 275 149 271 550 221 500 200 287 301
Nuclear Periphery 1 1 3 4 4 8 8 3 12 4 8 1 4 3 2 2 0 1 0 0 0 0 1
Nucleolus 43 21 83 42 80 177 291 156 284 168 169 22 36 36 18 10 9 7 5 24 18 46 63
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 5 10 2 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 1 1 0 0 0 1 2 15 18 0 0 0 1 0 3 1 1 1
Vac/Vac Membrane 2 4 7 0 11 4 11 1 7 5 5 5 2 11 8 6 11 12 5 17 20 47 80
Unique Cell Count 551 228 546 394 508 712 863 405 721 439 500 346 554 584 315 192 309 588 243 567 250 398 471
Labelled Cell Count 589 244 638 447 568 857 1052 540 969 588 665 365 593 620 337 205 322 588 243 567 250 398 471


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.0 12.9 8.1 9.4 9.9 8.0 8.5 7.9 7.9 8.1 7.9 11.3 12.2 11.8 12.8 12.0 12.5 11.7 12.6 12.7
Std Deviation (1e-4) 2.8 3.5 2.6 3.9 4.7 2.8 3.2 3.3 3.0 3.2 2.9 3.8 4.5 4.8 4.7 4.6 4.8 2.8 3.1 2.9
Intensity Change (Log2) 0.22 0.29 -0.02 0.07 -0.04 -0.04 0.0 -0.04 0.48 0.59 0.54 0.66 0.57 0.63 0.54 0.64 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 3.1 0 3.9 3.9 5.1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 3.1 -1.1 0.3 -0.8 -1.8 -0.7 -0.7 3.7 6.3 7.4 2.1 5.9 3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.5 -0.7 1.2 1.1 5.1 3.8 4.4 4.8 -4.6 -5.4 -6.3 -2.6 -1.2 -3.3
Nucleus -0.3 -4.7 -3.8 -7.9 -6.8 -7.2 -7.0 -7.1 -1.0 -3.9 -6.1 -1.5 -4.6 -1.3
Nuclear Periphery 0 0 0 0 0 1.8 0 1.7 0 0 0 0 0 0
Nucleolus -2.0 0.2 4.2 7.7 8.2 9.4 8.3 7.0 -4.0 -4.6 -4.9 -4.2 -3.6 -5.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 3.5 3.8 0 0 0
Vacuole 0 1.1 0 0 0 -0.5 -0.2 -0.4 0 0 0.8 1.4 0 2.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Smd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available