Standard name | |
---|---|
Human Ortholog | |
Description | Protein that exhibits physical and genetic interactions with Rpb8p; Rpb8p is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 6 | 10 | 3 | 2 | 2 | 3 | 3 | 0 | 0 | 0 | 2 | 5 | 9 | 3 | 0 | 1 | 3 | 5 | 20 | 2 | 9 | 24 |
Bud | 0 | 0 | 11 | 14 | 17 | 23 | 26 | 29 | 9 | 25 | 22 | 27 | 1 | 2 | 8 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 2 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 7 | 2 | 4 | 5 |
Bud Site | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 2 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 6 | 21 | 17 | 16 | 13 | 13 | 14 | 15 | 15 | 20 | 4 | 26 | 26 | 63 | 40 | 50 | 0 | 1 | 14 | 1 | 6 | 12 |
Cytoplasm | 3 | 11 | 138 | 166 | 228 | 237 | 309 | 374 | 210 | 284 | 186 | 209 | 8 | 84 | 113 | 347 | 231 | 241 | 3 | 16 | 27 | 4 | 19 | 10 |
Endoplasmic Reticulum | 13 | 6 | 14 | 12 | 20 | 17 | 13 | 6 | 13 | 14 | 3 | 10 | 4 | 14 | 8 | 4 | 5 | 11 | 54 | 29 | 87 | 18 | 65 | 80 |
Endosome | 0 | 0 | 2 | 0 | 8 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 0 | 0 | 21 | 77 | 68 | 121 | 174 | 235 | 212 | 275 | 165 | 280 | 1 | 1 | 11 | 4 | 2 | 1 | 2 | 6 | 46 | 4 | 16 | 13 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 9 | 8 | 14 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 1 | 2 | 3 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 3 | 3 | 3 | 3 | 10 | 17 | 3 | 12 | 6 | 16 | 0 | 0 | 0 | 19 | 42 | 17 | 3 | 5 | 10 | 3 | 10 | 4 |
Unique Cell Count | 16 | 16 | 185 | 249 | 319 | 359 | 457 | 573 | 392 | 510 | 309 | 440 | 16 | 111 | 143 | 392 | 276 | 279 | 73 | 72 | 223 | 39 | 141 | 156 |
Labelled Cell Count | 18 | 18 | 205 | 305 | 374 | 425 | 554 | 687 | 471 | 634 | 399 | 568 | 20 | 132 | 177 | 452 | 328 | 336 | 73 | 72 | 223 | 39 | 141 | 156 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 5.8 | 4.3 | 4.1 | 4.5 | 4.0 | 3.8 | 3.9 | 3.4 | 3.5 | 3.5 | 3.3 | 5.4 | 5.8 | 5.0 | 10.0 | 10.9 | 11.4 | – | 5.6 | 6.7 |
Std Deviation (1e-4) | 0.7 | 1.9 | 0.6 | 0.9 | 1.2 | 1.2 | 1.1 | 1.3 | 0.9 | 0.9 | 1.1 | 1.0 | 0.4 | 0.8 | 0.6 | 1.5 | 1.5 | 1.7 | – | 0.9 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.05 | -0.1 | -0.17 | -0.14 | -0.36 | -0.31 | -0.29 | -0.41 | 0.31 | 0.43 | 0.22 | 1.21 | 1.33 | 1.4 | – | 0.37 | 0.63 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.4 | 4.5 | 4.4 | 4.0 | 4.8 |
Cytoplasm | -1.8 | -0.8 | -2.0 | -1.7 | -2.4 | -4.8 | -4.5 | -3.3 | -6.2 | -2.1 | 0.2 | 0.9 | 4.3 | 2.4 | 3.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | -0.7 | -4.2 | -3.0 | -1.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.3 | -1.1 | -5.7 | -5.1 | -5.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 | 1.9 | 4.8 | 2.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8855 | 2.2839 | 1.9203 | 1.7866 | 1.56 | 2.13 | 2.4321 | 3.1906 | 3.3319 | 3.1749 | 2.9301 | 3.3489 | 2.0959 | 2.4753 | 2.3783 | 2.3825 | 2.1197 | 2.2434 |
Actin | 0.031 | 0.012 | 0.0233 | 0.0588 | 0.052 | 0.024 | 0.0277 | 0.0034 | 0.0073 | 0.0065 | 0.0147 | 0.0055 | 0.0059 | 0.0149 | 0.0136 | 0.0065 | 0.0123 | 0.0075 |
Bud | 0.0005 | 0.0003 | 0.0009 | 0.0002 | 0.0086 | 0.0004 | 0.0009 | 0.0002 | 0.0002 | 0.0015 | 0.0005 | 0.0011 | 0.0013 | 0.0279 | 0.003 | 0.0029 | 0.0002 | 0.0004 |
Bud Neck | 0.0102 | 0.0018 | 0.0047 | 0.0034 | 0.0166 | 0.0395 | 0.0119 | 0.0012 | 0.0017 | 0.0036 | 0.015 | 0.0301 | 0.0054 | 0.0023 | 0.0027 | 0.0009 | 0.0053 | 0.0184 |
Bud Periphery | 0.0015 | 0.0008 | 0.004 | 0.0012 | 0.0158 | 0.003 | 0.0018 | 0.0004 | 0.0008 | 0.0025 | 0.0008 | 0.0043 | 0.0007 | 0.007 | 0.0047 | 0.0023 | 0.0009 | 0.0006 |
Bud Site | 0.0094 | 0.0156 | 0.0126 | 0.0006 | 0.0379 | 0.0012 | 0.0182 | 0.0139 | 0.002 | 0.0016 | 0.0073 | 0.0007 | 0.0082 | 0.0561 | 0.0239 | 0.0014 | 0.0007 | 0.0004 |
Cell Periphery | 0.0244 | 0.024 | 0.0226 | 0.008 | 0.0084 | 0.0161 | 0.0101 | 0.0123 | 0.0136 | 0.0095 | 0.0065 | 0.0073 | 0.0222 | 0.0202 | 0.023 | 0.0071 | 0.0117 | 0.0101 |
Cytoplasm | 0.375 | 0.533 | 0.4392 | 0.2087 | 0.297 | 0.5019 | 0.3908 | 0.5764 | 0.6149 | 0.5119 | 0.4685 | 0.5745 | 0.4624 | 0.5779 | 0.4906 | 0.3762 | 0.5479 | 0.6038 |
Cytoplasmic Foci | 0.0317 | 0.0248 | 0.0262 | 0.0287 | 0.0378 | 0.0219 | 0.0367 | 0.0153 | 0.0162 | 0.0174 | 0.0912 | 0.0217 | 0.0299 | 0.0158 | 0.0215 | 0.0148 | 0.0342 | 0.0178 |
Eisosomes | 0.0018 | 0.001 | 0.0008 | 0.0006 | 0.0013 | 0.0007 | 0.0005 | 0.0004 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0011 | 0.0013 | 0.0012 | 0.001 | 0.0004 | 0.0005 |
Endoplasmic Reticulum | 0.3133 | 0.2393 | 0.3235 | 0.5055 | 0.3094 | 0.3088 | 0.2082 | 0.1719 | 0.2295 | 0.3019 | 0.219 | 0.2589 | 0.2332 | 0.0925 | 0.2121 | 0.3525 | 0.2407 | 0.1814 |
Endosome | 0.0396 | 0.0204 | 0.0271 | 0.036 | 0.032 | 0.0179 | 0.0343 | 0.0127 | 0.0165 | 0.0267 | 0.0568 | 0.0188 | 0.0151 | 0.0099 | 0.0116 | 0.0125 | 0.0217 | 0.01 |
Golgi | 0.0173 | 0.0068 | 0.0123 | 0.0092 | 0.0193 | 0.0056 | 0.0149 | 0.003 | 0.0091 | 0.0061 | 0.0146 | 0.0066 | 0.0057 | 0.0037 | 0.0061 | 0.0049 | 0.0056 | 0.0042 |
Lipid Particles | 0.0442 | 0.0736 | 0.034 | 0.0693 | 0.0519 | 0.0173 | 0.052 | 0.0147 | 0.017 | 0.0054 | 0.0189 | 0.0164 | 0.0369 | 0.0178 | 0.0218 | 0.0178 | 0.0339 | 0.0161 |
Mitochondria | 0.0045 | 0.0006 | 0.012 | 0.0445 | 0.0593 | 0.001 | 0.0038 | 0.0006 | 0.0012 | 0.0017 | 0.0016 | 0.0025 | 0.0027 | 0.0029 | 0.0011 | 0.0009 | 0.0017 | 0.0007 |
None | 0.046 | 0.0291 | 0.0321 | 0.0015 | 0.0121 | 0.0271 | 0.1625 | 0.1611 | 0.0541 | 0.092 | 0.0608 | 0.0421 | 0.1409 | 0.1371 | 0.149 | 0.1725 | 0.0719 | 0.1181 |
Nuclear Periphery | 0.0242 | 0.0048 | 0.0049 | 0.0052 | 0.0082 | 0.0041 | 0.0026 | 0.0024 | 0.0033 | 0.0038 | 0.0031 | 0.0017 | 0.0032 | 0.0027 | 0.0036 | 0.0063 | 0.0022 | 0.0017 |
Nucleolus | 0.0008 | 0.0003 | 0.0003 | 0.0001 | 0.0016 | 0.0002 | 0.0011 | 0.0009 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0032 | 0.0007 | 0.0006 | 0.0005 | 0.0001 | 0.0002 |
Nucleus | 0.0041 | 0.0017 | 0.0014 | 0.001 | 0.008 | 0.0016 | 0.0016 | 0.002 | 0.0011 | 0.0013 | 0.0014 | 0.001 | 0.0064 | 0.0023 | 0.0018 | 0.0028 | 0.0013 | 0.0015 |
Peroxisomes | 0.0064 | 0.0005 | 0.0085 | 0.0084 | 0.0053 | 0.0006 | 0.0071 | 0.0002 | 0.005 | 0.0007 | 0.0087 | 0.0026 | 0.0022 | 0.0005 | 0.001 | 0.0005 | 0.0005 | 0.0003 |
Punctate Nuclear | 0.0059 | 0.0018 | 0.0017 | 0.0009 | 0.0032 | 0.0015 | 0.0055 | 0.0011 | 0.0006 | 0.0006 | 0.0038 | 0.0007 | 0.0075 | 0.0019 | 0.0024 | 0.0104 | 0.0021 | 0.0029 |
Vacuole | 0.0066 | 0.0075 | 0.0073 | 0.007 | 0.0126 | 0.0053 | 0.0072 | 0.0056 | 0.0054 | 0.0045 | 0.0052 | 0.0028 | 0.0056 | 0.0044 | 0.0043 | 0.0048 | 0.0044 | 0.003 |
Vacuole Periphery | 0.0017 | 0.0003 | 0.0007 | 0.0012 | 0.0016 | 0.0004 | 0.0005 | 0.0003 | 0.0003 | 0.0005 | 0.0006 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.1498 | 18.5976 | 13.8002 | 19.0433 | 15.5225 | 13.3387 | 23.4164 | 17.0018 | 17.8746 | 21.3489 |
Translational Efficiency | 0.6943 | 0.4913 | 0.5534 | 0.3033 | 0.3787 | 0.497 | 0.3646 | 0.447 | 0.4149 | 0.3953 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
269 | 260 | 167 | 971 | 1586 | 1715 | 770 | 220 | 1855 | 1975 | 937 | 1191 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 717.56 | 906.89 | 1219.72 | 925.19 | 704.07 | 801.07 | 1045.61 | 1017.05 | 706.03 | 815.00 | 1076.64 | 942.16 |
Standard Deviation | 80.38 | 145.50 | 136.43 | 109.94 | 121.93 | 114.49 | 122.00 | 122.08 | 116.92 | 124.30 | 141.38 | 117.80 |
Intensity Change Log 2 | — | 0.337828 | 0.765379 | 0.366650 | — | 0.186209 | 0.570554 | 0.530600 | — | 0.264728 | 0.672175 | 0.450175 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003703 | 0.018917 | 0.016413 | 0.014749 | 0.002659 | 0.004320 | 0.018945 | 0.106726* | 0.002811 | 0.006241 | 0.018494 | 0.031739 |
Bud Neck | 0.021004 | 0.028112 | 0.007460 | 0.024491 | 0.007315 | 0.023824 | 0.012864 | 0.033894 | 0.009300 | 0.024389 | 0.011901 | 0.026228 |
Bud Site | 0.018075 | 0.035984 | 0.051992 | 0.057799 | 0.007321 | 0.024356 | 0.035548 | 0.134200* | 0.008881 | 0.025887 | 0.038479 | 0.071912 |
Cell Periphery | 0.008768 | 0.011063 | 0.003027 | 0.002429 | 0.003556 | 0.004625 | 0.007859 | 0.018952 | 0.004312 | 0.005473 | 0.006998 | 0.005481 |
Cytoplasm | 0.260751* | 0.243560* | 0.266440* | 0.263604* | 0.344190* | 0.333707* | 0.218733* | 0.203092* | 0.332090* | 0.321840* | 0.227236* | 0.252427* |
Cytoplasmic Foci | 0.162365 | 0.183014* | 0.092549 | 0.145180 | 0.153447 | 0.146248 | 0.053154 | 0.060228 | 0.154740 | 0.151088 | 0.060176 | 0.129488 |
Eisosomes | 0.007942 | 0.009223 | 0.000282 | 0.002533 | 0.006244 | 0.005632 | 0.004935 | 0.010977 | 0.006491 | 0.006105 | 0.004106 | 0.004093 |
Endoplasmic Reticulum | 0.178564* | 0.162800* | 0.220201* | 0.052675 | 0.159532* | 0.130073* | 0.320200* | 0.116437* | 0.162292* | 0.134382* | 0.302377* | 0.064453 |
Endosome | 0.027460 | 0.031675 | 0.028249 | 0.034831 | 0.019721 | 0.034382 | 0.014290 | 0.013731 | 0.020843 | 0.034025 | 0.016777 | 0.030934 |
Golgi | 0.072014 | 0.050759 | 0.005061 | 0.009408 | 0.069138 | 0.052234 | 0.007113 | 0.014605 | 0.069555 | 0.052040 | 0.006747 | 0.010368 |
Lipid Particles | 0.114715* | 0.073446 | 0.059175 | 0.058992 | 0.120165* | 0.099382 | 0.064135 | 0.047501 | 0.119374* | 0.095968 | 0.063251 | 0.056869 |
Mitochondria | 0.035378 | 0.007724 | 0.000070 | 0.002554 | 0.022756 | 0.022323 | 0.000810 | 0.001373 | 0.024586 | 0.020401 | 0.000678 | 0.002336 |
Mitotic Spindle | 0.000606 | 0.000783 | 0.001683 | 0.009262 | 0.000275 | 0.001596 | 0.001229 | 0.000502 | 0.000323 | 0.001489 | 0.001310 | 0.007644 |
None | 0.001807 | 0.000588 | 0.000377 | 0.000324 | 0.007157 | 0.003332 | 0.001144 | 0.000087 | 0.006381 | 0.002971 | 0.001007 | 0.000280 |
Nuclear Periphery | 0.000851 | 0.000331 | 0.001116 | 0.001837 | 0.001024 | 0.001260 | 0.002633 | 0.000285 | 0.000999 | 0.001138 | 0.002362 | 0.001550 |
Nuclear Periphery Foci | 0.002002 | 0.004093 | 0.079352 | 0.010995 | 0.002516 | 0.002037 | 0.037635 | 0.010208 | 0.002442 | 0.002307 | 0.045070 | 0.010850 |
Nucleolus | 0.000376 | 0.003458 | 0.000053 | 0.000432 | 0.000913 | 0.000801 | 0.000192 | 0.000090 | 0.000835 | 0.001151 | 0.000167 | 0.000369 |
Nucleus | 0.020280 | 0.025582 | 0.014547 | 0.041326 | 0.033027 | 0.035372 | 0.058581 | 0.029507 | 0.031179 | 0.034084 | 0.050733 | 0.039143 |
Peroxisomes | 0.028595 | 0.025989 | 0.000959 | 0.014079 | 0.017556 | 0.016961 | 0.003892 | 0.011092 | 0.019156 | 0.018149 | 0.003370 | 0.013527 |
Vacuole | 0.029430 | 0.079983 | 0.150548 | 0.250897* | 0.018096 | 0.052589 | 0.135636 | 0.185652 | 0.019739 | 0.056195 | 0.138294 | 0.238845* |
Vacuole Periphery | 0.005312 | 0.002917 | 0.000448 | 0.001604 | 0.003392 | 0.004946 | 0.000472 | 0.000860 | 0.003670 | 0.004679 | 0.000468 | 0.001466 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.16 | -2.02 | -5.44 | 1.33 | 0.38 | -2.64 | -6.56 | -7.81 | -7.67 | -6.40 | -4.67 | -6.73 | -9.39 | -8.02 | -3.15 |
Bud Neck | -1.14 | 3.92 | -0.52 | 0.91 | -7.07 | -9.95 | -4.32 | -6.35 | -1.90 | -4.75 | -9.06 | -2.15 | -8.23 | -0.10 | -6.45 |
Bud Site | -3.09 | -3.32 | -6.30 | -2.93 | -0.30 | -8.27 | -7.98 | -10.38 | -8.77 | -7.60 | -8.68 | -8.67 | -13.60 | -9.04 | -5.49 |
Cell Periphery | -0.64 | 3.21 | 3.65 | 2.95 | 1.64 | -1.80 | -4.62 | -5.84 | -5.32 | -4.01 | -1.65 | -3.10 | -1.02 | 0.63 | 2.01 |
Cytoplasm | 1.01 | -0.49 | 0.33 | -0.85 | 0.74 | 1.32 | 13.13 | 10.22 | 9.54 | 1.51 | 1.40 | 11.71 | 10.07 | 8.93 | -1.77 |
Cytoplasmic Foci | -1.49 | 5.47 | 1.89 | 3.41 | -4.87 | 1.35 | 20.17 | 12.86 | 12.26 | -1.14 | 0.73 | 20.11 | 4.76 | 4.24 | -12.84 |
Eisosomes | -0.66 | 5.57 | 4.05 | 5.27 | -8.27 | 1.12 | 2.37 | -2.58 | -3.09 | -3.66 | 0.73 | 4.39 | 5.18 | 5.21 | 0.38 |
Endoplasmic Reticulum | 0.80 | -1.04 | 8.26 | 7.34 | 7.47 | 3.97 | -11.35 | 3.77 | 1.59 | 11.58 | 3.99 | -10.83 | 15.62 | 11.84 | 20.79 |
Endosome | -1.00 | 0.02 | -1.50 | -0.42 | -1.66 | -7.83 | 3.96 | 2.70 | 7.70 | 0.52 | -7.68 | 3.20 | -4.08 | 2.08 | -6.26 |
Golgi | 2.95 | 12.79 | 11.86 | 8.04 | -2.73 | 5.77 | 27.46 | 10.77 | 7.66 | -1.29 | 6.46 | 30.34 | 25.08 | 18.94 | -2.04 |
Lipid Particles | 4.04 | 5.20 | 6.71 | 2.42 | 0.19 | 4.09 | 10.53 | 9.24 | 6.90 | 1.72 | 5.08 | 11.72 | 13.47 | 9.74 | 1.54 |
Mitochondria | 4.60 | 6.23 | 5.74 | 2.32 | -2.12 | 0.22 | 15.31 | 13.85 | 13.29 | -0.60 | 2.17 | 16.19 | 13.30 | 11.84 | -1.60 |
Mitotic Spindle | -0.55 | -1.12 | -4.35 | -4.22 | -3.59 | -2.47 | -2.48 | -2.32 | 1.80 | 1.56 | -2.52 | -2.76 | -4.62 | -3.77 | -3.93 |
None | 1.15 | 1.41 | 1.47 | 0.68 | 0.12 | 2.80 | 5.04 | 6.33 | 4.64 | 2.08 | 2.88 | 5.23 | 6.30 | 4.40 | 1.68 |
Nuclear Periphery | 2.88 | -0.74 | -2.81 | -4.44 | -1.98 | -1.15 | -4.65 | 4.73 | 5.67 | 7.01 | -0.77 | -4.66 | -2.16 | -1.72 | 1.62 |
Nuclear Periphery Foci | -3.28 | -9.91 | -10.01 | -6.77 | 8.63 | 1.61 | -13.60 | -6.40 | -6.79 | 8.70 | 0.51 | -16.46 | -11.06 | -11.42 | 12.37 |
Nucleolus | -0.97 | 4.49 | -0.64 | 0.95 | -3.90 | 0.27 | 2.37 | 2.71 | 5.54 | 2.88 | -0.66 | 2.56 | 1.64 | 1.79 | -2.52 |
Nucleus | -1.31 | 0.99 | -6.13 | -4.44 | -8.15 | -1.11 | -7.55 | 0.43 | 0.97 | 5.63 | -1.53 | -6.91 | -3.88 | -2.45 | 3.80 |
Peroxisomes | 0.44 | 10.20 | 4.82 | 3.05 | -7.56 | 0.34 | 13.83 | 3.71 | 3.50 | -3.49 | 0.62 | 16.94 | 3.93 | 3.37 | -6.70 |
Vacuole | -6.54 | -9.48 | -27.25 | -17.58 | -7.24 | -14.35 | -19.11 | -12.55 | -10.35 | -3.71 | -15.59 | -21.37 | -32.48 | -26.73 | -10.98 |
Vacuole Periphery | 3.01 | 7.60 | 4.63 | 2.01 | -1.83 | -3.21 | 8.65 | 6.32 | 7.83 | -0.77 | -2.31 | 10.72 | 4.71 | 5.83 | -1.78 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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