Standard name
Human Ortholog
Description Protein that exhibits physical and genetic interactions with Rpb8p; Rpb8p is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0 0 0 0 0 0 0 0 0 0 0 0.12 0.05 0.06 0 0 0 0.05 0.07 0.09 0.07 0.07 0.16
Bud 0 0 0.06 0.06 0.05 0.06 0.06 0.05 0 0.05 0.07 0.06 0.06 0 0.06 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.06 0 0.08 0.05 0 0 0 0 0 0.05 0.05 0.25 0.23 0.18 0.16 0.14 0.18 0 0 0.06 0.05 0.05 0.08
Cytoplasm 0.19 0.69 0.75 0.67 0.71 0.66 0.68 0.65 0.54 0.56 0.6 0.47 0.5 0.76 0.79 0.89 0.84 0.86 0 0.23 0.12 0.12 0.14 0.07
Endoplasmic Reticulum 0.81 0.38 0.08 0.05 0.06 0.05 0 0 0 0 0 0 0.25 0.13 0.06 0 0 0 0.75 0.42 0.39 0.46 0.46 0.51
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.11 0.31 0.21 0.34 0.38 0.41 0.54 0.54 0.53 0.64 0.06 0 0.08 0 0 0 0 0.09 0.21 0.1 0.12 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.15 0.06 0 0.08 0.05 0.08 0.08 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 6 10 3 2 2 3 3 0 0 0 2 5 9 3 0 1 3 5 20 2 9 24
Bud 0 0 11 14 17 23 26 29 9 25 22 27 1 2 8 0 0 0 0 0 1 0 1 0
Bud Neck 0 0 2 0 2 2 0 1 0 0 0 1 0 0 0 0 0 0 2 3 7 2 4 5
Bud Site 0 0 1 0 1 0 1 2 2 2 2 2 0 0 0 0 0 0
Cell Periphery 0 1 6 21 17 16 13 13 14 15 15 20 4 26 26 63 40 50 0 1 14 1 6 12
Cytoplasm 3 11 138 166 228 237 309 374 210 284 186 209 8 84 113 347 231 241 3 16 27 4 19 10
Endoplasmic Reticulum 13 6 14 12 20 17 13 6 13 14 3 10 4 14 8 4 5 11 54 29 87 18 65 80
Endosome 0 0 2 0 8 1 2 2 1 0 0 0 0 0 0 2 0 0 0 0 2 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1
Mitochondria 0 0 21 77 68 121 174 235 212 275 165 280 1 1 11 4 2 1 2 6 46 4 16 13
Nucleus 0 0 0 1 0 2 2 3 2 2 0 0 0 0 0 9 8 14 0 0 0 0 0 0
Nuclear Periphery 1 0 1 1 0 0 1 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 4 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 3 1 1 1 2 3 0 1 0 0 2 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 3 3 3 3 10 17 3 12 6 16 0 0 0 19 42 17 3 5 10 3 10 4
Unique Cell Count 16 16 185 249 319 359 457 573 392 510 309 440 16 111 143 392 276 279 73 72 223 39 141 156
Labelled Cell Count 18 18 205 305 374 425 554 687 471 634 399 568 20 132 177 452 328 336 73 72 223 39 141 156


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 5.8 4.3 4.1 4.5 4.0 3.8 3.9 3.4 3.5 3.5 3.3 5.4 5.8 5.0 10.0 10.9 11.4 5.6 6.7
Std Deviation (1e-4) 0.7 1.9 0.6 0.9 1.2 1.2 1.1 1.3 0.9 0.9 1.1 1.0 0.4 0.8 0.6 1.5 1.5 1.7 0.9 1.6
Intensity Change (Log2) -0.06 0.05 -0.1 -0.17 -0.14 -0.36 -0.31 -0.29 -0.41 0.31 0.43 0.22 1.21 1.33 1.4 0.37 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1.3 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 -0.1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 5.4 4.5 4.4 4.0 4.8
Cytoplasm -1.8 -0.8 -2.0 -1.7 -2.4 -4.8 -4.5 -3.3 -6.2 -2.1 0.2 0.9 4.3 2.4 3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1.4 -0.7 -4.2 -3.0 -1.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 -3.3 -1.1 -5.7 -5.1 -5.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 1.3 0 0 0 1.9 4.8 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8855 2.2839 1.9203 1.7866 1.56 2.13 2.4321 3.1906 3.3319 3.1749 2.9301 3.3489 2.0959 2.4753 2.3783 2.3825 2.1197 2.2434
Actin 0.031 0.012 0.0233 0.0588 0.052 0.024 0.0277 0.0034 0.0073 0.0065 0.0147 0.0055 0.0059 0.0149 0.0136 0.0065 0.0123 0.0075
Bud 0.0005 0.0003 0.0009 0.0002 0.0086 0.0004 0.0009 0.0002 0.0002 0.0015 0.0005 0.0011 0.0013 0.0279 0.003 0.0029 0.0002 0.0004
Bud Neck 0.0102 0.0018 0.0047 0.0034 0.0166 0.0395 0.0119 0.0012 0.0017 0.0036 0.015 0.0301 0.0054 0.0023 0.0027 0.0009 0.0053 0.0184
Bud Periphery 0.0015 0.0008 0.004 0.0012 0.0158 0.003 0.0018 0.0004 0.0008 0.0025 0.0008 0.0043 0.0007 0.007 0.0047 0.0023 0.0009 0.0006
Bud Site 0.0094 0.0156 0.0126 0.0006 0.0379 0.0012 0.0182 0.0139 0.002 0.0016 0.0073 0.0007 0.0082 0.0561 0.0239 0.0014 0.0007 0.0004
Cell Periphery 0.0244 0.024 0.0226 0.008 0.0084 0.0161 0.0101 0.0123 0.0136 0.0095 0.0065 0.0073 0.0222 0.0202 0.023 0.0071 0.0117 0.0101
Cytoplasm 0.375 0.533 0.4392 0.2087 0.297 0.5019 0.3908 0.5764 0.6149 0.5119 0.4685 0.5745 0.4624 0.5779 0.4906 0.3762 0.5479 0.6038
Cytoplasmic Foci 0.0317 0.0248 0.0262 0.0287 0.0378 0.0219 0.0367 0.0153 0.0162 0.0174 0.0912 0.0217 0.0299 0.0158 0.0215 0.0148 0.0342 0.0178
Eisosomes 0.0018 0.001 0.0008 0.0006 0.0013 0.0007 0.0005 0.0004 0.0003 0.0002 0.0003 0.0002 0.0011 0.0013 0.0012 0.001 0.0004 0.0005
Endoplasmic Reticulum 0.3133 0.2393 0.3235 0.5055 0.3094 0.3088 0.2082 0.1719 0.2295 0.3019 0.219 0.2589 0.2332 0.0925 0.2121 0.3525 0.2407 0.1814
Endosome 0.0396 0.0204 0.0271 0.036 0.032 0.0179 0.0343 0.0127 0.0165 0.0267 0.0568 0.0188 0.0151 0.0099 0.0116 0.0125 0.0217 0.01
Golgi 0.0173 0.0068 0.0123 0.0092 0.0193 0.0056 0.0149 0.003 0.0091 0.0061 0.0146 0.0066 0.0057 0.0037 0.0061 0.0049 0.0056 0.0042
Lipid Particles 0.0442 0.0736 0.034 0.0693 0.0519 0.0173 0.052 0.0147 0.017 0.0054 0.0189 0.0164 0.0369 0.0178 0.0218 0.0178 0.0339 0.0161
Mitochondria 0.0045 0.0006 0.012 0.0445 0.0593 0.001 0.0038 0.0006 0.0012 0.0017 0.0016 0.0025 0.0027 0.0029 0.0011 0.0009 0.0017 0.0007
None 0.046 0.0291 0.0321 0.0015 0.0121 0.0271 0.1625 0.1611 0.0541 0.092 0.0608 0.0421 0.1409 0.1371 0.149 0.1725 0.0719 0.1181
Nuclear Periphery 0.0242 0.0048 0.0049 0.0052 0.0082 0.0041 0.0026 0.0024 0.0033 0.0038 0.0031 0.0017 0.0032 0.0027 0.0036 0.0063 0.0022 0.0017
Nucleolus 0.0008 0.0003 0.0003 0.0001 0.0016 0.0002 0.0011 0.0009 0.0001 0.0001 0.0005 0.0001 0.0032 0.0007 0.0006 0.0005 0.0001 0.0002
Nucleus 0.0041 0.0017 0.0014 0.001 0.008 0.0016 0.0016 0.002 0.0011 0.0013 0.0014 0.001 0.0064 0.0023 0.0018 0.0028 0.0013 0.0015
Peroxisomes 0.0064 0.0005 0.0085 0.0084 0.0053 0.0006 0.0071 0.0002 0.005 0.0007 0.0087 0.0026 0.0022 0.0005 0.001 0.0005 0.0005 0.0003
Punctate Nuclear 0.0059 0.0018 0.0017 0.0009 0.0032 0.0015 0.0055 0.0011 0.0006 0.0006 0.0038 0.0007 0.0075 0.0019 0.0024 0.0104 0.0021 0.0029
Vacuole 0.0066 0.0075 0.0073 0.007 0.0126 0.0053 0.0072 0.0056 0.0054 0.0045 0.0052 0.0028 0.0056 0.0044 0.0043 0.0048 0.0044 0.003
Vacuole Periphery 0.0017 0.0003 0.0007 0.0012 0.0016 0.0004 0.0005 0.0003 0.0003 0.0005 0.0006 0.0003 0.0003 0.0003 0.0003 0.0005 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.1498 18.5976 13.8002 19.0433 15.5225 13.3387 23.4164 17.0018 17.8746 21.3489
Translational Efficiency 0.6943 0.4913 0.5534 0.3033 0.3787 0.497 0.3646 0.447 0.4149 0.3953

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
269 260 167 971 1586 1715 770 220 1855 1975 937 1191

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 717.56 906.89 1219.72 925.19 704.07 801.07 1045.61 1017.05 706.03 815.00 1076.64 942.16
Standard Deviation 80.38 145.50 136.43 109.94 121.93 114.49 122.00 122.08 116.92 124.30 141.38 117.80
Intensity Change Log 2 0.337828 0.765379 0.366650 0.186209 0.570554 0.530600 0.264728 0.672175 0.450175

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003703 0.018917 0.016413 0.014749 0.002659 0.004320 0.018945 0.106726* 0.002811 0.006241 0.018494 0.031739
Bud Neck 0.021004 0.028112 0.007460 0.024491 0.007315 0.023824 0.012864 0.033894 0.009300 0.024389 0.011901 0.026228
Bud Site 0.018075 0.035984 0.051992 0.057799 0.007321 0.024356 0.035548 0.134200* 0.008881 0.025887 0.038479 0.071912
Cell Periphery 0.008768 0.011063 0.003027 0.002429 0.003556 0.004625 0.007859 0.018952 0.004312 0.005473 0.006998 0.005481
Cytoplasm 0.260751* 0.243560* 0.266440* 0.263604* 0.344190* 0.333707* 0.218733* 0.203092* 0.332090* 0.321840* 0.227236* 0.252427*
Cytoplasmic Foci 0.162365 0.183014* 0.092549 0.145180 0.153447 0.146248 0.053154 0.060228 0.154740 0.151088 0.060176 0.129488
Eisosomes 0.007942 0.009223 0.000282 0.002533 0.006244 0.005632 0.004935 0.010977 0.006491 0.006105 0.004106 0.004093
Endoplasmic Reticulum 0.178564* 0.162800* 0.220201* 0.052675 0.159532* 0.130073* 0.320200* 0.116437* 0.162292* 0.134382* 0.302377* 0.064453
Endosome 0.027460 0.031675 0.028249 0.034831 0.019721 0.034382 0.014290 0.013731 0.020843 0.034025 0.016777 0.030934
Golgi 0.072014 0.050759 0.005061 0.009408 0.069138 0.052234 0.007113 0.014605 0.069555 0.052040 0.006747 0.010368
Lipid Particles 0.114715* 0.073446 0.059175 0.058992 0.120165* 0.099382 0.064135 0.047501 0.119374* 0.095968 0.063251 0.056869
Mitochondria 0.035378 0.007724 0.000070 0.002554 0.022756 0.022323 0.000810 0.001373 0.024586 0.020401 0.000678 0.002336
Mitotic Spindle 0.000606 0.000783 0.001683 0.009262 0.000275 0.001596 0.001229 0.000502 0.000323 0.001489 0.001310 0.007644
None 0.001807 0.000588 0.000377 0.000324 0.007157 0.003332 0.001144 0.000087 0.006381 0.002971 0.001007 0.000280
Nuclear Periphery 0.000851 0.000331 0.001116 0.001837 0.001024 0.001260 0.002633 0.000285 0.000999 0.001138 0.002362 0.001550
Nuclear Periphery Foci 0.002002 0.004093 0.079352 0.010995 0.002516 0.002037 0.037635 0.010208 0.002442 0.002307 0.045070 0.010850
Nucleolus 0.000376 0.003458 0.000053 0.000432 0.000913 0.000801 0.000192 0.000090 0.000835 0.001151 0.000167 0.000369
Nucleus 0.020280 0.025582 0.014547 0.041326 0.033027 0.035372 0.058581 0.029507 0.031179 0.034084 0.050733 0.039143
Peroxisomes 0.028595 0.025989 0.000959 0.014079 0.017556 0.016961 0.003892 0.011092 0.019156 0.018149 0.003370 0.013527
Vacuole 0.029430 0.079983 0.150548 0.250897* 0.018096 0.052589 0.135636 0.185652 0.019739 0.056195 0.138294 0.238845*
Vacuole Periphery 0.005312 0.002917 0.000448 0.001604 0.003392 0.004946 0.000472 0.000860 0.003670 0.004679 0.000468 0.001466

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.16 -2.02 -5.44 1.33 0.38 -2.64 -6.56 -7.81 -7.67 -6.40 -4.67 -6.73 -9.39 -8.02 -3.15
Bud Neck -1.14 3.92 -0.52 0.91 -7.07 -9.95 -4.32 -6.35 -1.90 -4.75 -9.06 -2.15 -8.23 -0.10 -6.45
Bud Site -3.09 -3.32 -6.30 -2.93 -0.30 -8.27 -7.98 -10.38 -8.77 -7.60 -8.68 -8.67 -13.60 -9.04 -5.49
Cell Periphery -0.64 3.21 3.65 2.95 1.64 -1.80 -4.62 -5.84 -5.32 -4.01 -1.65 -3.10 -1.02 0.63 2.01
Cytoplasm 1.01 -0.49 0.33 -0.85 0.74 1.32 13.13 10.22 9.54 1.51 1.40 11.71 10.07 8.93 -1.77
Cytoplasmic Foci -1.49 5.47 1.89 3.41 -4.87 1.35 20.17 12.86 12.26 -1.14 0.73 20.11 4.76 4.24 -12.84
Eisosomes -0.66 5.57 4.05 5.27 -8.27 1.12 2.37 -2.58 -3.09 -3.66 0.73 4.39 5.18 5.21 0.38
Endoplasmic Reticulum 0.80 -1.04 8.26 7.34 7.47 3.97 -11.35 3.77 1.59 11.58 3.99 -10.83 15.62 11.84 20.79
Endosome -1.00 0.02 -1.50 -0.42 -1.66 -7.83 3.96 2.70 7.70 0.52 -7.68 3.20 -4.08 2.08 -6.26
Golgi 2.95 12.79 11.86 8.04 -2.73 5.77 27.46 10.77 7.66 -1.29 6.46 30.34 25.08 18.94 -2.04
Lipid Particles 4.04 5.20 6.71 2.42 0.19 4.09 10.53 9.24 6.90 1.72 5.08 11.72 13.47 9.74 1.54
Mitochondria 4.60 6.23 5.74 2.32 -2.12 0.22 15.31 13.85 13.29 -0.60 2.17 16.19 13.30 11.84 -1.60
Mitotic Spindle -0.55 -1.12 -4.35 -4.22 -3.59 -2.47 -2.48 -2.32 1.80 1.56 -2.52 -2.76 -4.62 -3.77 -3.93
None 1.15 1.41 1.47 0.68 0.12 2.80 5.04 6.33 4.64 2.08 2.88 5.23 6.30 4.40 1.68
Nuclear Periphery 2.88 -0.74 -2.81 -4.44 -1.98 -1.15 -4.65 4.73 5.67 7.01 -0.77 -4.66 -2.16 -1.72 1.62
Nuclear Periphery Foci -3.28 -9.91 -10.01 -6.77 8.63 1.61 -13.60 -6.40 -6.79 8.70 0.51 -16.46 -11.06 -11.42 12.37
Nucleolus -0.97 4.49 -0.64 0.95 -3.90 0.27 2.37 2.71 5.54 2.88 -0.66 2.56 1.64 1.79 -2.52
Nucleus -1.31 0.99 -6.13 -4.44 -8.15 -1.11 -7.55 0.43 0.97 5.63 -1.53 -6.91 -3.88 -2.45 3.80
Peroxisomes 0.44 10.20 4.82 3.05 -7.56 0.34 13.83 3.71 3.50 -3.49 0.62 16.94 3.93 3.37 -6.70
Vacuole -6.54 -9.48 -27.25 -17.58 -7.24 -14.35 -19.11 -12.55 -10.35 -3.71 -15.59 -21.37 -32.48 -26.73 -10.98
Vacuole Periphery 3.01 7.60 4.63 2.01 -1.83 -3.21 8.65 6.32 7.83 -0.77 -2.31 10.72 4.71 5.83 -1.78