Standard name
Description Protein that exhibits physical and genetic interactions with Rpb8p; Rpb8p is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0 0 0 0 0 0 0 0 0 0 0 0.12 0.05 0.06 0 0 0 0.05 0.07 0.09 0.07 0.07 0.16
Bud 0 0 0.06 0.06 0.05 0.06 0.06 0.05 0 0.05 0.07 0.06 0.06 0 0.06 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.06 0 0.08 0.05 0 0 0 0 0 0.05 0.05 0.25 0.23 0.18 0.16 0.14 0.18 0 0 0.06 0.05 0.05 0.08
Cytoplasm 0.19 0.69 0.75 0.67 0.71 0.66 0.68 0.65 0.54 0.56 0.6 0.47 0.5 0.76 0.79 0.89 0.84 0.86 0 0.23 0.12 0.12 0.14 0.07
Endoplasmic Reticulum 0.81 0.38 0.08 0.05 0.06 0.05 0 0 0 0 0 0 0.25 0.13 0.06 0 0 0 0.75 0.42 0.39 0.46 0.46 0.51
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.11 0.31 0.21 0.34 0.38 0.41 0.54 0.54 0.53 0.64 0.06 0 0.08 0 0 0 0 0.09 0.21 0.1 0.12 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.15 0.06 0 0.08 0.05 0.08 0.08 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 6 10 3 2 2 3 3 0 0 0 2 5 9 3 0 1 3 5 20 2 9 24
Bud 0 0 11 14 17 23 26 29 9 25 22 27 1 2 8 0 0 0 0 0 1 0 1 0
Bud Neck 0 0 2 0 2 2 0 1 0 0 0 1 0 0 0 0 0 0 2 3 7 2 4 5
Bud Site 0 0 1 0 1 0 1 2 2 2 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 6 21 17 16 13 13 14 15 15 20 4 26 26 63 40 50 0 1 14 1 6 12
Cytoplasm 3 11 138 166 228 237 309 374 210 284 186 209 8 84 113 347 231 241 3 16 27 4 19 10
Endoplasmic Reticulum 13 6 14 12 20 17 13 6 13 14 3 10 4 14 8 4 5 11 54 29 87 18 65 80
Endosome 0 0 2 0 8 1 2 2 1 0 0 0 0 0 0 2 0 0 0 0 2 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1
Mitochondria 0 0 21 77 68 121 174 235 212 275 165 280 1 1 11 4 2 1 2 6 46 4 16 13
Nucleus 0 0 0 1 0 2 2 3 2 2 0 0 0 0 0 9 8 14 0 0 0 0 0 0
Nuclear Periphery 1 0 1 1 0 0 1 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 4 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 3 1 1 1 2 3 0 1 0 0 2 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 3 3 3 3 10 17 3 12 6 16 0 0 0 19 42 17 3 5 10 3 10 4
Unique Cell Count 16 16 185 249 319 359 457 573 392 510 309 440 16 111 143 392 276 279 73 72 223 39 141 156
Labelled Cell Count 18 18 205 305 374 425 554 687 471 634 399 568 20 132 177 452 328 336 73 72 223 39 141 156


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 5.8 4.3 4.1 4.5 4.0 3.8 3.9 3.4 3.5 3.5 3.3 5.4 5.8 5.0 10.0 10.9 11.4 – 5.6 6.7
Std Deviation (1e-4) 0.7 1.9 0.6 0.9 1.2 1.2 1.1 1.3 0.9 0.9 1.1 1.0 0.4 0.8 0.6 1.5 1.5 1.7 – 0.9 1.6
Intensity Change (Log2) – – – -0.06 0.05 -0.1 -0.17 -0.14 -0.36 -0.31 -0.29 -0.41 0.31 0.43 0.22 1.21 1.33 1.4 – 0.37 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1.3 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 -0.1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 5.4 4.5 4.4 4.0 4.8
Cytoplasm -1.8 -0.8 -2.0 -1.7 -2.4 -4.8 -4.5 -3.3 -6.2 -2.1 0.2 0.9 4.3 2.4 3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1.4 -0.7 -4.2 -3.0 -1.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 -3.3 -1.1 -5.7 -5.1 -5.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 1.3 0 0 0 1.9 4.8 2.3