Standard name
Description Mitochondrial membrane transporter; mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud 0.05 0 0 0 0 0 0 0 – 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.52 0.74 0.58 0.66 0.65 0.55 0.38 0.67 – 0.22 0.31 0.31 0.89 0.94 0.96 0.75 0.69 0.71 0.15 0.22 0.16 0.08 0.07 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.09 0.08 0.09 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.11 0.05 0.05 0.09 0 0.06 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.55 0.15 0.35 0.34 0.34 0.59 0.79 0.41 – 0.84 0.85 0.81 0 0 0 0.08 0.07 0.07 0.64 0.43 0.62 0.61 0.61 0.58
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 – 0 0 0.05 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.07 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0.1 0 0.07 0 0.09 0.09 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 2 0 0 0 0 0 – 0 0 0 4 7 1 3 2 0 0 33 10 16 16 26
Bud 2 7 3 1 3 3 10 3 – 5 6 17 4 0 0 2 3 0 1 10 0 5 6 21
Bud Neck 0 4 4 0 1 5 1 0 – 2 1 0 8 10 3 3 4 1 0 12 1 12 17 20
Bud Site 0 0 0 0 0 0 1 2 – 2 1 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 10 6 0 0 1 0 2 – 5 1 2 5 6 3 6 4 7 0 8 3 2 5 6
Cytoplasm 23 369 104 61 114 132 102 135 – 59 66 94 477 620 642 198 150 87 8 150 38 45 37 65
Endoplasmic Reticulum 1 9 6 1 0 1 4 0 – 4 3 2 2 6 5 23 17 11 0 11 4 10 6 10
Endosome 0 23 0 1 1 0 0 0 – 0 0 0 16 10 9 14 24 6 2 60 7 31 23 24
Golgi 0 2 0 0 0 0 0 0 – 0 0 0 0 2 2 2 2 2 0 4 0 1 5 5
Mitochondria 24 74 63 31 59 141 210 84 – 224 181 249 2 3 0 20 15 9 38 297 148 346 315 390
Nucleus 0 1 0 0 1 2 2 2 – 0 2 4 0 3 0 2 1 2 0 0 0 1 1 0
Nuclear Periphery 0 0 0 1 0 0 0 0 – 2 2 0 0 0 1 1 1 0 0 2 1 1 0 3
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Peroxisomes 0 1 0 2 4 0 0 2 – 0 0 0 4 1 1 3 8 3 0 1 2 3 3 4
SpindlePole 0 9 9 3 6 6 1 9 – 10 3 14 8 1 3 15 11 4 0 17 5 22 18 18
Vac/Vac Membrane 2 34 3 0 7 4 1 2 – 4 1 1 26 11 12 8 8 12 1 49 9 53 44 64
Unique Cell Count 44 498 179 92 175 240 267 203 267 214 308 538 661 666 264 217 123 59 689 241 571 514 675
Labelled Cell Count 52 545 200 101 196 295 332 241 317 267 387 556 680 682 300 250 144 59 689 241 571 514 675


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.2 3.5 3.8 3.3 3.1 2.6 3.9 – 2.6 2.6 2.6 5.3 5.2 5.2 5.5 5.4 5.9 5.6 5.7 5.9
Std Deviation (1e-4) 2.4 0.9 0.6 1.5 1.2 0.9 1.2 11.6 – 0.3 0.5 0.4 0.8 0.9 1.0 1.3 1.3 1.9 1.1 1.1 1.4
Intensity Change (Log2) – – – 0.14 -0.06 -0.14 -0.39 0.18 – -0.43 -0.43 -0.41 0.62 0.57 0.58 0.66 0.63 0.77 0.68 0.71 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 3.2