Standard name | |
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Description | Regulator of the Fps1p glycerol channel; under nonstress conditions, binds to Fps1p to positively regulate glycerol transport; under osmotic stress, multiple phosphorylation by Hog1p causes Ask10p to dissociate from Fps1p; forms homodimers and heterodimerizes with paralog Rgc1p; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; associates with RNA polymerase II holoenzyme |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 12 | 5 | 7 | 29 | 74 | 38 | 51 | 50 | 30 | – | 7 | 6 | 16 | 34 | 33 | 4 | 2 | 6 | 33 | 15 | 20 | 10 | 22 | 33 |
Bud | 9 | 7 | 10 | 10 | 38 | 40 | 28 | 42 | 27 | – | 24 | 33 | 15 | 26 | 38 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 5 | 4 | 12 | 17 | 18 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 75 | 57 | 32 | 91 | 133 | 95 | 132 | 91 | 102 | – | 37 | 84 | 149 | 205 | 230 | 24 | 13 | 41 | 109 | 58 | 51 | 42 | 92 | 141 |
Cytoplasm | 145 | 84 | 67 | 98 | 146 | 192 | 301 | 360 | 261 | – | 152 | 286 | 167 | 274 | 370 | 37 | 30 | 59 | 49 | 41 | 23 | 57 | 147 | 155 |
Endoplasmic Reticulum | 6 | 2 | 1 | 0 | 3 | 4 | 5 | 10 | 5 | – | 6 | 7 | 4 | 2 | 4 | 2 | 2 | 0 | 22 | 2 | 5 | 4 | 15 | 26 |
Endosome | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 2 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 1 | 0 | 0 | 1 |
Mitochondria | 1 | 6 | 4 | 16 | 31 | 75 | 137 | 149 | 161 | – | 116 | 245 | 1 | 2 | 9 | 1 | 2 | 0 | 15 | 1 | 7 | 1 | 4 | 3 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | – | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 5 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 4 | 1 | 2 | 1 | 3 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 6 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 10 | 3 | 1 | 2 | 3 | 4 |
Unique Cell Count | 216 | 123 | 94 | 158 | 277 | 294 | 478 | 544 | 427 | 254 | 484 | 242 | 370 | 472 | 51 | 40 | 96 | 266 | 136 | 118 | 137 | 313 | 392 | |
Labelled Cell Count | 267 | 163 | 121 | 245 | 432 | 448 | 661 | 712 | 594 | 346 | 663 | 352 | 544 | 685 | 68 | 51 | 117 | 266 | 136 | 118 | 137 | 313 | 392 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.3 | 7.4 | 5.3 | 5.7 | 6.1 | 5.4 | 5.2 | 5.2 | 4.4 | – | 4.1 | 4.0 | 8.9 | 8.3 | 7.1 | 11.0 | 11.0 | 13.0 | 8.2 | 8.4 | 9.1 |
Std Deviation (1e-4) | 1.6 | 1.4 | 1.0 | 1.0 | 1.3 | 1.0 | 1.1 | 1.2 | 0.8 | – | 0.6 | 0.8 | 1.7 | 1.4 | 1.2 | 2.4 | 2.7 | 2.5 | 1.5 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.2 | 0.02 | -0.02 | -0.03 | -0.27 | – | -0.36 | -0.43 | 0.75 | 0.64 | 0.41 | 1.05 | 1.04 | 1.29 | 0.62 | 0.66 | 0.77 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.4 | 3.9 | 1.4 | 0.9 | 0.5 | 0 | – | 0 | 0 | -0.3 | 0.5 | -0.2 | 0 | 0 | -0.3 |
Bud | -1.2 | 0.8 | 0.7 | -1.7 | -1.0 | 0 | – | 0 | 0 | -1.4 | -1.2 | -0.8 | 0 | 0 | -2.8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 3.6 | 2.4 | -0.3 | -1.3 | -3.9 | 0 | – | 0 | 0 | 4.5 | 3.7 | 2.6 | 1.5 | -0.2 | 1.2 |
Cytoplasm | -1.5 | -3.1 | -1.1 | -1.5 | -1.0 | -1.8 | – | -2.0 | -2.2 | -0.4 | 0.5 | 1.5 | 0.2 | 0.4 | -1.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.7 | 2.0 | 4.5 | 5.0 | 4.9 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |