Standard name
Description Regulator of the Fps1p glycerol channel; under nonstress conditions, binds to Fps1p to positively regulate glycerol transport; under osmotic stress, multiple phosphorylation by Hog1p causes Ask10p to dissociate from Fps1p; forms homodimers and heterodimerizes with paralog Rgc1p; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; associates with RNA polymerase II holoenzyme
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0 0.07 0.18 0.27 0.13 0.11 0.09 0.07 – 0 0 0.07 0.09 0.07 0.08 0.05 0.06 0.12 0.12 0.18 0.08 0.07 0.08
Bud 0 0.06 0.11 0.06 0.14 0.14 0.06 0.08 0.06 – 0.09 0.07 0.06 0.07 0.08 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.05
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.35 0.46 0.34 0.58 0.48 0.32 0.28 0.17 0.24 – 0.15 0.17 0.62 0.55 0.49 0.47 0.33 0.43 0.41 0.43 0.44 0.31 0.29 0.36
Cytoplasm 0.67 0.68 0.71 0.62 0.53 0.65 0.63 0.66 0.61 – 0.6 0.59 0.69 0.74 0.78 0.73 0.75 0.61 0.18 0.3 0.2 0.42 0.47 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.08 0 0 0 0.05 0.07
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.1 0.11 0.26 0.29 0.27 0.38 – 0.46 0.51 0 0 0 0 0.05 0 0.06 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 12 5 7 29 74 38 51 50 30 – 7 6 16 34 33 4 2 6 33 15 20 10 22 33
Bud 9 7 10 10 38 40 28 42 27 – 24 33 15 26 38 0 1 1 1 1 0 0 3 2
Bud Neck 0 0 0 0 0 2 1 1 0 – 0 1 0 0 0 0 0 0 8 5 4 12 17 18
Bud Site 0 0 0 0 0 1 1 1 1 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 75 57 32 91 133 95 132 91 102 – 37 84 149 205 230 24 13 41 109 58 51 42 92 141
Cytoplasm 145 84 67 98 146 192 301 360 261 – 152 286 167 274 370 37 30 59 49 41 23 57 147 155
Endoplasmic Reticulum 6 2 1 0 3 4 5 10 5 – 6 7 4 2 4 2 2 0 22 2 5 4 15 26
Endosome 4 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 4 1 0 2 1 2
Golgi 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 2 3 0 1 0 0 1
Mitochondria 1 6 4 16 31 75 137 149 161 – 116 245 1 2 9 1 2 0 15 1 7 1 4 3
Nucleus 0 1 0 0 0 0 1 3 2 – 2 1 0 0 1 0 0 4 0 0 0 0 0 0
Nuclear Periphery 5 1 0 1 0 0 0 1 1 – 0 0 0 0 0 0 0 0 2 0 0 0 0 0
Nucleolus 3 0 0 0 1 0 0 1 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 4 1 2 1 3 – 2 0 0 0 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 6 0 0 0 2 0 0 2 1 – 0 0 0 0 0 0 0 3 10 3 1 2 3 4
Unique Cell Count 216 123 94 158 277 294 478 544 427 254 484 242 370 472 51 40 96 266 136 118 137 313 392
Labelled Cell Count 267 163 121 245 432 448 661 712 594 346 663 352 544 685 68 51 117 266 136 118 137 313 392


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 7.4 5.3 5.7 6.1 5.4 5.2 5.2 4.4 – 4.1 4.0 8.9 8.3 7.1 11.0 11.0 13.0 8.2 8.4 9.1
Std Deviation (1e-4) 1.6 1.4 1.0 1.0 1.3 1.0 1.1 1.2 0.8 – 0.6 0.8 1.7 1.4 1.2 2.4 2.7 2.5 1.5 1.7 1.7
Intensity Change (Log2) – – – 0.09 0.2 0.02 -0.02 -0.03 -0.27 – -0.36 -0.43 0.75 0.64 0.41 1.05 1.04 1.29 0.62 0.66 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.4 3.9 1.4 0.9 0.5 0 – 0 0 -0.3 0.5 -0.2 0 0 -0.3
Bud -1.2 0.8 0.7 -1.7 -1.0 0 – 0 0 -1.4 -1.2 -0.8 0 0 -2.8
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 3.6 2.4 -0.3 -1.3 -3.9 0 – 0 0 4.5 3.7 2.6 1.5 -0.2 1.2
Cytoplasm -1.5 -3.1 -1.1 -1.5 -1.0 -1.8 – -2.0 -2.2 -0.4 0.5 1.5 0.2 0.4 -1.4
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 1.7 2.0 4.5 5.0 4.9 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0