Standard name
Description Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0 0 0 0 0 0 0 0 0 0.07 0 0 0.06 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.08 0.06 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.06 0.08 0.07 0.09 0.05 0.06 0 0.05 0.08 0 0.11 0.12 0.15 0.15 0 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0 0.06 0.08 0.12 0 0 0 0 0 0
Endosome 0.06 0.07 0.11 0.26 0.27 0 0.19 0.1 0.06 0.05 0 0 0.12 0.2 0.3 0.27 0.19 0.35 0.11 0.08 0.09 0.08 0.1 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0.06 0.11 0 0.05 0 0 0 0.07 0.08
Mitochondria 0.87 0.86 0.44 0.65 0.49 0.86 0.67 0.79 0.89 0.84 0.85 0.9 0.61 0.51 0.3 0.08 0.19 0.12 0.68 0.75 0.77 0.69 0.64 0.63
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0.06 0.05 0.23 0.12 0 0.05 0 0 0.06 0 0 0.07 0.1 0.22 0.14 0.11 0.12 0.07 0 0.05 0.07 0.06 0.06
SpindlePole 0 0 0.2 0.1 0.15 0.06 0.1 0.07 0.06 0.1 0.05 0.05 0.08 0 0.05 0.13 0.14 0.15 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.11 0.11 0.07 0.19 0.07 0.13 0.08 0.07 0.05 0.05 0 0.05 0.09 0.1 0.22 0.17 0.23 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 6 7 0 2 0 0 0 0 1 0 0 21 12 7 6 0 0 3 9 2 5 19 20
Bud 4 20 6 6 3 9 4 16 12 21 11 11 5 4 8 4 1 1 0 4 0 1 3 1
Bud Neck 0 0 2 0 2 0 0 0 0 2 0 1 0 0 0 0 1 0 0 2 0 1 8 7
Bud Site 2 0 0 1 0 5 2 5 2 9 5 6 0 0 0 0 0 2 – – – – – –
Cell Periphery 6 20 5 10 6 0 5 5 1 4 1 1 14 21 19 8 2 0 0 1 0 1 0 2
Cytoplasm 6 26 8 19 18 27 17 31 10 18 20 8 32 54 77 15 1 3 0 2 0 3 1 1
Endoplasmic Reticulum 2 8 6 0 2 3 1 1 2 1 1 1 14 20 18 6 3 3 0 1 0 0 1 5
Endosome 15 34 14 64 70 11 69 49 24 17 7 2 36 89 153 27 7 9 30 39 18 33 59 59
Golgi 4 5 3 2 4 0 3 1 1 0 0 0 24 22 14 6 4 0 12 20 4 13 40 45
Mitochondria 218 414 54 161 128 268 236 404 344 303 203 196 184 226 150 8 7 3 184 394 166 290 381 384
Nucleus 2 0 0 1 6 12 10 12 4 11 3 4 0 1 1 6 0 0 0 0 0 0 1 3
Nuclear Periphery 6 1 0 0 0 1 13 11 5 6 3 5 4 1 0 1 0 0 5 0 1 6 6 5
Nucleolus 2 0 1 1 0 2 1 11 9 6 3 5 0 0 0 0 1 0 0 1 0 2 1 2
Peroxisomes 12 27 6 58 31 5 19 19 15 20 3 3 20 43 110 14 4 3 19 17 11 30 38 39
SpindlePole 9 10 25 24 39 20 34 38 23 36 13 11 25 12 23 13 5 4 7 5 2 10 15 5
Vac/Vac Membrane 28 53 14 17 49 21 45 40 27 17 13 7 14 40 51 22 6 6 3 19 2 15 16 15
Unique Cell Count 252 480 124 247 260 313 354 509 385 362 239 218 304 446 503 101 36 26 272 526 216 422 600 606
Labelled Cell Count 316 624 151 364 360 384 459 643 479 472 286 261 393 545 631 136 42 34 272 526 216 422 600 606


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.2 6.0 5.6 6.1 4.5 5.5 5.6 4.9 5.1 5.2 4.9 5.6 5.7 5.7 6.2 6.4 6.0 4.9 4.9 5.2
Std Deviation (1e-4) 0.8 0.8 1.3 1.4 1.5 1.3 1.3 1.6 1.1 1.6 1.7 1.3 1.0 1.0 1.1 1.3 1.1 1.7 1.2 1.1 1.4
Intensity Change (Log2) – – – -0.1 0.02 -0.42 -0.12 -0.1 -0.27 -0.22 -0.21 -0.28 -0.09 -0.08 -0.06 0.06 0.11 0.02 -0.29 -0.28 -0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0.5 0 0 0.1 0 0
Bud 0 0 0 0 0 0 0.4 -0.1 0.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.3 0.3 0 1.2 0 0
Cytoplasm 0.4 0.2 0.8 -0.7 -0.2 0 -0.6 0.6 -1.2 1.3 1.8 2.6 2.1 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 -0.1 -0.2 0 0.4 0 0
Endosome 3.3 3.5 0 2.1 -0.6 -1.9 -2.6 -3.2 -4.4 0.2 2.2 4.3 3.0 0 0
Golgi 0 0 0 0 0 0 0 0 0 2.1 1.2 0 0 0 0
Mitochondria 4.0 1.0 0 4.5 8.0 10.7 8.7 8.2 9.3 3.2 1.4 -2.9 -5.9 -2.6 -3.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 4.5 2.2 0 0.2 0 -0.5 0.3 0 0 0.7 1.7 4.4 2.4 0 0
SpindlePole -2.8 -1.3 0 -3.1 -4.2 -4.7 -3.0 -4.3 -4.4 -3.5 -7.0 -5.8 -1.5 -0.8 -0.6
Vacuole -1.4 1.9 -1.6 0.4 -1.2 -1.5 -2.6 -2.0 -3.0 -2.5 -0.8 -0.4 2.1 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5951 0.8679 0.6955 0.4996 0.3928 0.9935 1.7733 2.459 2.0434 1.9662 1.8023 2.199 4.9348 5.046 4.4964 5.5289 4.9248 4.7978
Actin 0.0059 0.0026 0.0087 0.0048 0.0021 0.0032 0.0028 0.0045 0.0037 0.0193 0.0305 0.0034 0.0246 0.0299 0.0418 0.0262 0.0019 0.0172
Bud 0.0006 0.0017 0.0006 0.0007 0.0012 0.0003 0.0004 0.0009 0.0009 0.0024 0.0017 0.0005 0.0011 0.0035 0.0044 0.0006 0.0012 0.0007
Bud Neck 0.0005 0.0004 0.0011 0.0037 0.0004 0.0005 0.0007 0.0006 0.0004 0.0007 0.0004 0.0008 0.0007 0.0038 0.0009 0.0012 0.0006 0.0044
Bud Periphery 0.0018 0.0026 0.002 0.0016 0.0054 0.0012 0.0008 0.0018 0.0018 0.0094 0.006 0.0011 0.0019 0.0052 0.0066 0.0016 0.0058 0.0013
Bud Site 0.0015 0.0049 0.0027 0.0029 0.0019 0.0005 0.0012 0.0052 0.0025 0.0024 0.0014 0.0006 0.0034 0.0563 0.0092 0.0029 0.0019 0.0015
Cell Periphery 0.0006 0.0006 0.0006 0.0004 0.0003 0.0004 0.0004 0.0009 0.0004 0.0009 0.0004 0.0002 0.0009 0.0049 0.0018 0.0005 0.0197 0.0007
Cytoplasm 0.0047 0.0066 0.0026 0.0008 0.0024 0.0023 0.0051 0.0036 0.0109 0.0047 0.0048 0.0041 0.0105 0.0256 0.0184 0.001 0.0143 0.0163
Cytoplasmic Foci 0.0362 0.039 0.0326 0.0184 0.0453 0.0233 0.0328 0.0294 0.0212 0.0218 0.0505 0.0277 0.0512 0.0427 0.0437 0.027 0.0451 0.0448
Eisosomes 0.0004 0.0004 0.0005 0.0004 0.0002 0.0003 0.0002 0.0006 0.0003 0.0005 0.0005 0.0002 0.0007 0.0038 0.0013 0.0003 0.0006 0.0004
Endoplasmic Reticulum 0.002 0.0015 0.0004 0.0002 0.001 0.001 0.0021 0.0009 0.0007 0.0017 0.0006 0.001 0.0104 0.0019 0.0042 0.0003 0.0041 0.0024
Endosome 0.0979 0.0866 0.0511 0.0318 0.0698 0.0493 0.11 0.0732 0.0566 0.0475 0.065 0.0502 0.1038 0.0825 0.0656 0.0302 0.0919 0.0748
Golgi 0.0612 0.0642 0.0535 0.0262 0.0421 0.0463 0.066 0.072 0.0607 0.0392 0.0597 0.0641 0.085 0.0692 0.053 0.0368 0.1022 0.0746
Lipid Particles 0.0316 0.0187 0.0167 0.0037 0.0048 0.0278 0.0298 0.0064 0.0084 0.0068 0.007 0.0088 0.0649 0.0107 0.017 0.0144 0.0542 0.0364
Mitochondria 0.6964 0.7027 0.7554 0.8703 0.7766 0.7809 0.6991 0.7612 0.7849 0.8139 0.7161 0.7883 0.5018 0.5829 0.6752 0.7695 0.538 0.6359
None 0.003 0.0034 0.0095 0.0007 0.0006 0.0042 0.0036 0.0014 0.0028 0.0025 0.001 0.0026 0.0182 0.0072 0.0081 0.001 0.0018 0.0182
Nuclear Periphery 0.0039 0.0014 0.0003 0.0001 0.0012 0.0037 0.0008 0.0006 0.0002 0.0002 0.0002 0.0019 0.0237 0.0003 0.0034 0.0002 0.0245 0.0009
Nucleolus 0.0006 0.0005 0.0007 0.0003 0.0002 0.0009 0.0005 0.0002 0.0002 0.0002 0.0002 0.0001 0.0017 0.0008 0.0012 0.0002 0.0006 0.0009
Nucleus 0.0005 0.0006 0.0005 0.0003 0.0006 0.0017 0.0007 0.0005 0.0003 0.0003 0.0002 0.0004 0.0032 0.0007 0.0126 0.0002 0.0226 0.0006
Peroxisomes 0.032 0.0406 0.0523 0.0289 0.0355 0.0433 0.0306 0.0297 0.0348 0.0202 0.0472 0.0388 0.0651 0.052 0.0208 0.0803 0.0463 0.0589
Punctate Nuclear 0.0003 0.0002 0.0005 0.0002 0.0004 0.0014 0.0005 0.0002 0.0002 0.0001 0.0002 0.0001 0.0076 0.0012 0.0017 0.0002 0.0021 0.0014
Vacuole 0.011 0.0118 0.0046 0.0021 0.0039 0.0035 0.0071 0.0035 0.0035 0.0026 0.0037 0.0024 0.0096 0.0105 0.0047 0.0026 0.0117 0.0046
Vacuole Periphery 0.0075 0.0089 0.0031 0.0016 0.0041 0.0041 0.0048 0.003 0.0047 0.0023 0.0031 0.0029 0.0101 0.0045 0.0045 0.0029 0.0089 0.0032

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.9865 28.5906 17.2376 21.4605 19.4377 9.9891 20.3259 19.5109 19.096 18.9165
Translational Efficiency 0.9302 0.9523 1.148 0.6866 1.2251 1.8038 1.034 1.0571 1.142 1.3749

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘