Standard name
Description ER protein that functions in assembly of the V0 sector of V-ATPase; functions with other assembly factors; null mutation enhances the vacuolar ATPase (V-ATPase) deficiency of a vma21 mutant impaired in endoplasmic reticulum (ER) retrieval
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0 0 0.05 0.08 0.07 0.08 0.13 0.09 0.16 0.14 0 0 0 0.05 0.08 0.06 0.07 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 0.1 0.1 0.14 0.14 0.11 0.15 0.14 0.12 0 0 0 0.05 0 0.07 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0.08 0.06 0.07 0.1 0.11 0.07 0.11 0.1 0.13 0.09 0.08 0.11 0.08 0.12 0.18 0.12 0.16 0 0 0 0 0 0
Mitochondria – 0 0.09 0 0.1 0.15 0.12 0.1 0.14 0.11 0.18 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0.1 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0.1 0 0 0.05 0
Nuclear Periphery – 0 0.11 0.11 0.07 0.12 0.21 0.12 0.11 0.06 0.06 0.11 0 0 0.09 0.05 0 0 0.07 0 0.13 0.05 0.05 0
Nucleolus – 0.1 0.09 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.88 0.78 0.87 0.67 0.56 0.51 0.52 0.52 0.52 0.46 0.44 0.92 0.94 0.94 0.92 0.88 0.86 0.74 0.81 0.79 0.86 0.8 0.84
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 8 11 14 27 17 28 11 15 0 0 0 0 0 1 0 0 0 0 0 0
Bud – 0 0 0 3 2 2 1 1 3 2 2 0 0 0 0 0 0 0 0 0 0 4 4
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 1 0 1 4 6 18 21 24 40 20 23 0 0 2 0 0 2 0 0 0 0 0 0
Cytoplasm – 3 4 5 19 35 46 38 61 55 67 76 9 5 7 13 14 13 2 1 0 0 2 0
Endoplasmic Reticulum – 0 1 4 39 45 89 65 52 97 58 65 1 2 8 12 5 15 0 0 1 2 3 4
Endosome – 0 0 0 1 0 1 1 0 0 1 2 0 0 0 0 0 0 0 0 1 4 5 1
Golgi – 6 8 16 37 47 46 54 46 83 40 45 36 33 53 45 22 33 0 0 0 0 2 3
Mitochondria – 0 12 5 36 67 75 48 62 73 77 99 1 2 0 10 2 5 0 0 1 2 8 6
Nucleus – 7 6 3 11 13 4 6 3 11 3 2 20 5 3 6 5 3 1 8 3 5 19 17
Nuclear Periphery – 0 15 25 26 51 134 57 52 40 24 60 5 10 37 12 6 8 2 3 20 11 20 16
Nucleolus – 7 13 17 19 18 19 16 6 8 4 6 10 17 6 13 9 14 0 1 2 4 8 8
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane – 64 108 189 250 248 320 246 238 332 196 236 301 378 400 227 157 174 29 73 124 206 316 336
Unique Cell Count 73 138 218 375 443 633 475 459 638 425 536 326 403 426 247 179 202 40 91 158 240 397 402
Labelled Cell Count 88 167 265 455 543 768 580 562 770 503 631 383 452 516 338 220 268 40 91 158 240 397 402


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 53.2 31.7 32.4 25.7 23.2 21.4 22.1 21.5 20.5 20.4 19.9 40.4 38.1 38.5 39.7 40.8 43.2 42.6 41.9 45.4
Std Deviation (1e-4) – 15.1 10.2 8.6 7.6 7.2 6.5 6.5 6.0 5.8 5.8 5.8 10.3 11.5 10.9 14.2 13.6 14.5 12.6 14.6 14.8
Intensity Change (Log2) – – – 0.03 -0.3 -0.45 -0.56 -0.52 -0.56 -0.63 -0.64 -0.67 0.35 0.26 0.28 0.32 0.36 0.44 0.42 0.4 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 2.9 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 2.7 3.0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.1 2.1 1.9 2.1 3.4 2.3 4.0 3.7 0 0 0 1.1 1.9 1.5
Endoplasmic Reticulum 0 3.6 3.6 4.4 4.3 3.8 4.6 4.3 4.0 0 0 0 0 0 2.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.6 1.4 1.7 0.6 1.9 1.5 2.4 1.3 1.0 1.8 0.9 2.2 3.4 2.0 2.9
Mitochondria -2.8 0.3 1.9 1.1 0.5 1.5 0.9 2.6 2.8 0 0 0 -1.9 -3.3 -2.6
Nucleus 0 0 0 0 0 0 0 0 0 0.8 0 0 0 0 0
Nuclear Periphery 0.2 -1.5 0.2 2.8 0.4 0.1 -1.9 -2.1 0.1 -4.5 -4.1 -0.8 -2.2 -2.7 -2.5
Nucleolus -0.5 -1.8 -2.4 -3.4 -2.9 0 0 0 0 -2.9 -2.3 0 -1.6 -1.5 -0.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.1 -2.5 -4.7 -5.9 -5.5 -5.5 -5.6 -6.6 -7.2 4.3 5.2 5.3 3.8 2.3 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 29.6992 33.0353 33.4652 31.5079 28.3522 31.9527 34.6029 37.1854 34.8655 35.2574 34.1552 35.4984 33.7136 37.1017 35.3851 34.0049 34.5 34.1781
Actin 0.0062 0.0001 0 0.0105 0.0005 0.0002 0.002 0 0 0 0 0.0009 0.0042 0 0.0034 0 0 0.0388
Bud 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0.0001 0.0001 0 0.0001 0.0001 0 0.0001
Bud Neck 0.0001 0.0008 0.0002 0.0001 0 0 0.0001 0 0 0 0 0.0001 0.0001 0 0.0005 0.0005 0 0.0004
Bud Periphery 0.0003 0.0002 0.0003 0.0003 0.0002 0.0003 0.0002 0.0001 0.0002 0.0002 0.0001 0.0002 0.0004 0.0001 0.0002 0.0003 0.0001 0.0002
Bud Site 0.0004 0.0039 0.0001 0.0002 0.0003 0.0001 0.0002 0 0 0 0 0.0001 0.0001 0 0.002 0.0001 0 0.0002
Cell Periphery 0.0028 0.0023 0.0026 0.0003 0.0022 0.002 0.0026 0.0029 0.0018 0.0006 0.0004 0.0007 0.0048 0.0023 0.0028 0.0015 0.0061 0.0004
Cytoplasm 0.0011 0.0006 0.0025 0.0002 0.0016 0.002 0.0007 0.0001 0.0003 0 0 0.0003 0.0019 0 0.0027 0.0001 0 0.0003
Cytoplasmic Foci 0.0163 0.0002 0.0011 0.0019 0.0277 0.0098 0.0045 0 0.0004 0 0 0.0014 0.0059 0 0.0022 0.0001 0.0001 0.0012
Eisosomes 0 0 0 0.0001 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0 0 0.0001
Endoplasmic Reticulum 0.0008 0.0001 0.0003 0.0004 0.0008 0.0005 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001 0.0003 0.0001 0 0.0005
Endosome 0.0098 0.0014 0.0082 0.0062 0.0184 0.0024 0.0037 0.0011 0.0018 0.0008 0.0009 0.0042 0.004 0.0012 0.0037 0.0047 0.0015 0.0028
Golgi 0.0024 0.0001 0.0004 0.003 0.0034 0.0003 0.0007 0 0.0001 0 0 0.0033 0.0008 0 0.0007 0.0007 0 0.0024
Lipid Particles 0.0072 0.0001 0.0011 0.0063 0.0251 0.0029 0.0021 0.0002 0.0006 0.0001 0.0001 0.0023 0.0099 0.0003 0.0031 0.0005 0.001 0.003
Mitochondria 0.0037 0.0002 0.0012 0.0399 0.0032 0.0002 0.002 0.0005 0.001 0.0007 0.0001 0.0057 0.0106 0.0003 0.0067 0.0044 0.0006 0.0004
None 0.001 0 0 0.0013 0.0061 0.0013 0.0019 0 0.0001 0 0 0.0001 0.0005 0 0.0003 0 0 0.0003
Nuclear Periphery 0.0048 0.0135 0.0081 0.0224 0.0058 0.0268 0.007 0.0036 0.0049 0.0019 0.0046 0.0109 0.0028 0.0092 0.0214 0.0016 0.0023 0.079
Nucleolus 0.009 0.005 0.006 0.0001 0.0054 0.0006 0.0033 0.0003 0.0012 0.0001 0.0001 0.0011 0.0052 0.0012 0.0048 0.0007 0.0196 0.0003
Nucleus 0.0035 0.0071 0.0084 0.0004 0.0033 0.0009 0.0015 0.0007 0.0011 0.0003 0.0002 0.0009 0.0037 0.0026 0.0053 0.0007 0.0029 0.0006
Peroxisomes 0.0062 0 0.0001 0.0046 0.0019 0.0002 0.0016 0 0.0001 0 0 0.0016 0.0016 0 0.0005 0.0001 0 0.0011
Punctate Nuclear 0.002 0 0 0.001 0.0066 0.0023 0.0011 0 0.0003 0 0 0.0001 0.0006 0 0.0024 0 0 0.0021
Vacuole 0.2957 0.2887 0.3064 0.2595 0.2507 0.2029 0.242 0.2269 0.2259 0.2315 0.1667 0.1302 0.2806 0.2554 0.2722 0.2601 0.2478 0.1374
Vacuole Periphery 0.6264 0.6755 0.6527 0.6412 0.6368 0.7441 0.7223 0.7635 0.7601 0.7636 0.8267 0.8357 0.6619 0.7273 0.6648 0.7237 0.7178 0.7286

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 161.7773 158.2157 166.9322 202.9207 173.8965 110.5557 176.2189 186.839 173.4817 203.6279
Translational Efficiency 1.3699 1.4029 1.519 1.3029 1.2017 1.9275 1.4025 1.1884 1.4126 1.1205

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘