TheCellVision

CLB1

Standard name	CLB1 ORF YGR108W Aliases B-type cyclin CLB1, SCB1
Human Ortholog	CCNB2 CCNB1
Description	B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB1 has a paralog, CLB2, that arose from the whole genome duplication

DOWNLOAD

Scale images

CLB1

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Neck	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cytoplasm	–	0.91	0.95	0.85	0.84	0.76	0.7	0.66	0.58	0.63	0.45	0.46	0.7	0.81	0.87	0.93	0.89	0.93	0.39	0.63	0.63	0.89	0.84	0.77
Endoplasmic Reticulum	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.07	0.13	0.09	0.24	0	0	0	0	0
Endosome	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Golgi	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	–	0.05	0	0.14	0.07	0.38	0.38	0.37	0.6	0.54	0.66	0.71	0	0	0	0	0	0	0.12	0.1	0.19	0	0	0
Nucleus	–	0	0	0	0	0	0	0	0	0	0	0	0.13	0	0	0	0	0	0	0	0	0	0	0
Nuclear Periphery	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vac/Vac Membrane	–	0.06	0	0.05	0.09	0	0	0	0	0	0	0	0.18	0.17	0.14	0	0.09	0.05	0.05	0.07	0	0	0	0.06

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	3	4	5	1	3	5
Bud	–	0	2	2	0	1	1	6	2	5	7	4	0	0	0	0	0	0	0	3	1	0	6	5
Bud Neck	–	0	1	0	1	1	0	1	2	3	0	0	0	0	0	0	1	0	0	0	1	0	1	4
Bud Site	–	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	–	0	0	1	2	1	3	1	2	0	4	3	0	0	0	4	7	4	0	1	1	0	0	1
Cytoplasm	–	158	195	138	202	233	366	314	173	219	156	198	124	260	306	253	214	179	37	123	136	97	251	180
Endoplasmic Reticulum	–	3	1	0	0	4	1	3	2	2	3	6	1	0	1	20	31	17	23	1	1	0	1	1
Endosome	–	0	3	2	6	2	8	12	1	2	1	0	5	11	9	6	3	1	3	4	6	2	3	1
Golgi	–	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	1	0	2	2	0	1	2
Mitochondria	–	8	8	22	17	116	199	174	179	190	232	305	0	0	0	6	2	0	11	20	40	4	13	7
Nucleus	–	0	0	2	2	0	8	16	8	5	14	10	23	9	2	3	3	2	0	8	0	0	0	0
Nuclear Periphery	–	0	0	0	0	0	2	1	0	1	1	0	0	0	0	1	0	0	0	0	0	0	0	0
Nucleolus	–	0	0	1	2	0	0	0	0	1	0	0	2	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	–	0	0	1	0	0	3	2	0	0	3	1	0	0	0	1	0	0	0	0	0	0	1	0
SpindlePole	–	1	0	0	2	4	6	8	1	6	5	4	0	0	0	1	0	1	0	4	3	0	5	2
Vac/Vac Membrane	–	10	2	8	21	5	16	10	7	10	13	8	31	53	49	10	22	10	5	13	9	0	4	13
Unique Cell Count		174	205	162	241	307	522	475	298	349	349	432	176	320	351	273	241	192	97	195	217	110	300	233
Labelled Cell Count		181	212	177	255	368	613	548	377	444	440	540	186	333	367	305	284	215	97	195	217	110	300	233

Yeast GFP Assignment

Nucleus

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	–	4.1	3.8	3.7	4.0	3.2	2.9	3.2	3.0	2.8	2.9	2.9	4.9	4.4	4.4	4.9	5.7	5.5	4.2	4.2	5.1
Std Deviation (1e^-4)	–	1.1	1.1	1.7	1.9	1.0	1.1	1.1	1.1	1.2	0.6	1.6	1.6	1.1	1.2	1.4	1.2	1.5	1.7	1.6	2.0
Intensity Change (Log₂)	–	–	–	-0.05	0.06	-0.24	-0.39	-0.25	-0.35	-0.46	-0.4	-0.39	0.34	0.19	0.21	0.35	0.56	0.52	0.11	0.11	0.42

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Actin	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Bud	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Bud Neck	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Bud Site	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Cell Periphery	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Cyto	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Endoplasmic Reticulum	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Endosome	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Golgi	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Mitochondria	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Nuclear Periphery	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Nuc	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Nucleolus	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Peroxisomes	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
SpindlePole	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Vac	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Cortical Patches	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Cytoplasm	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Nucleus	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Vacuole	–	–	–	–	–	–	–	–	–	–	–	–	1.9	3.8	2.5

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	-1.144	-0.4624	-0.5799	-0.3061	-0.5342	-0.8507	2.005	1.6163	2.0598	2.1598	2.1241	1.4704	2.9538	2.9836	2.9849	3.3805	3.0031	2.4226
Actin	0.0243	0.0035	0.0018	0.0298	0.003	0.0017	0.0301	0.0005	0.0506	0.0202	0.0187	0.0021	0.0597	0.0015	0.0088	0.0122	0.0064	0.0029
Bud	0.0023	0.0011	0.0002	0.0002	0.0002	0.0001	0.0014	0.0013	0.0058	0.0098	0.0019	0.0009	0.0028	0.0047	0.0011	0.0142	0.0098	0.0017
Bud Neck	0.0061	0.0003	0.0003	0.0011	0.0012	0.0007	0.0028	0.0001	0.0017	0.0011	0.0116	0.0001	0.0035	0.0005	0.0025	0.0007	0.0006	0.0004
Bud Periphery	0.0049	0.0013	0.0008	0.0002	0.0001	0.0001	0.0033	0.0049	0.0039	0.0248	0.0025	0.0033	0.0029	0.0046	0.0013	0.0082	0.0064	0.0042
Bud Site	0.0058	0.0036	0.0004	0.0008	0.0003	0.0001	0.0121	0.0031	0.0098	0.0152	0.0346	0.0001	0.0057	0.0064	0.0151	0.0022	0.0009	0.0002
Cell Periphery	0.0003	0.0001	0.0002	0.0002	0.0001	0	0.0008	0.0003	0.0006	0.0009	0.0006	0.0001	0.0005	0.0008	0.0011	0.0005	0.0003	0.0001
Cytoplasm	0.0369	0.1295	0.1007	0.2393	0.2248	0.0834	0.0631	0.0761	0.0836	0.1821	0.2139	0.0514	0.0514	0.09	0.1097	0.1925	0.1305	0.076
Cytoplasmic Foci	0.0138	0.0074	0.0078	0.0026	0.0231	0.011	0.0615	0.0046	0.0265	0.0053	0.0379	0.0054	0.0162	0.0101	0.0196	0.0247	0.0206	0.011
Eisosomes	0.0008	0.0002	0.0004	0.0002	0.0001	0.0002	0.0009	0.0003	0.0005	0.0005	0.0002	0.0001	0.0006	0.0015	0.0002	0.0003	0.0002	0.0001
Endoplasmic Reticulum	0.0037	0.0024	0.0032	0.0036	0.0041	0.0005	0.0063	0.0009	0.0034	0.0016	0.0038	0.0005	0.006	0.0012	0.0031	0.0026	0.0102	0.0007
Endosome	0.014	0.0016	0.0044	0.0027	0.0112	0.0013	0.0252	0.0012	0.0107	0.0052	0.0146	0.001	0.01	0.0046	0.0076	0.0084	0.0102	0.0038
Golgi	0.004	0.0004	0.0004	0.0009	0.0011	0.0006	0.0167	0.0001	0.0098	0.0034	0.008	0.0018	0.0054	0.0035	0.002	0.0017	0.0008	0.0029
Lipid Particles	0.0075	0.0006	0.0021	0.0005	0.0018	0.0043	0.0164	0.0007	0.0067	0.003	0.0042	0.0017	0.0096	0.0012	0.0034	0.0045	0.003	0.007
Mitochondria	0.0071	0.0007	0.0132	0.0007	0.001	0.0039	0.0158	0.0008	0.0063	0.0326	0.005	0.0088	0.0051	0.0011	0.0016	0.0034	0.0024	0.0012
None	0.822	0.7064	0.7164	0.4476	0.6614	0.8624	0.6961	0.8647	0.6466	0.4487	0.5064	0.9094	0.772	0.8385	0.6439	0.5137	0.7303	0.8635
Nuclear Periphery	0.0107	0.0113	0.0086	0.0121	0.0192	0.0005	0.0034	0.0011	0.0078	0.0033	0.031	0.0004	0.0118	0.0011	0.0096	0.0059	0.0145	0.0011
Nucleolus	0.0016	0.0016	0.0022	0.0007	0.0008	0.0006	0.0055	0.0005	0.0024	0.0019	0.0011	0.0006	0.001	0.0007	0.0012	0.0042	0.0013	0.0003
Nucleus	0.0112	0.1112	0.1147	0.2458	0.0224	0.0128	0.0039	0.0353	0.0856	0.2258	0.0425	0.0102	0.0058	0.0131	0.1499	0.1609	0.0421	0.0153
Peroxisomes	0.0131	0.0043	0.0032	0.001	0.0012	0.0076	0.0215	0.0004	0.021	0.0029	0.042	0.0011	0.0169	0.0023	0.0053	0.0024	0.0006	0.0036
Punctate Nuclear	0.0054	0.01	0.0169	0.0076	0.021	0.0079	0.0084	0.0018	0.011	0.0067	0.0163	0.0003	0.0097	0.0013	0.0104	0.0333	0.0037	0.0033
Vacuole	0.0029	0.0022	0.0017	0.0018	0.0015	0.0004	0.0039	0.001	0.0048	0.0039	0.0019	0.0004	0.002	0.0107	0.0019	0.0025	0.0038	0.0005
Vacuole Periphery	0.0015	0.0003	0.0006	0.0004	0.0006	0.0001	0.001	0.0001	0.0007	0.0013	0.0014	0.0002	0.0012	0.0005	0.0007	0.001	0.0014	0.0002

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	1.5585	25.0774	21.7099	19.1822	10.6084	1.5576	33.6315	35.6529	27.0473	12.4699
Translational Efficiency	–	–	–	–	–	–	–	–	–	–

Hit Data

Dataset	Hit
Protein Concentration	✘
Protein Localization	✘
Gene Expression	✔
Translational Efficiency	–

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
1738	1330	1656	538	1015	1633	1601	113	2753	2963	3257	651

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	644.18	658.24	777.71	810.23	650.31	667.16	842.77	743.17	646.44	663.16	809.69	798.59
Standard Deviation	68.77	95.50	94.23	106.78	75.83	88.33	97.59	108.39	71.52	91.73	101.26	110.04
Intensity Change Log ₂	—	0.031150	0.271768	0.330868	—	0.036905	0.374011	0.192565	—	0.034044	0.324037	0.263046

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.000029	0.000223	0.000150	0.000683	0.000047	0.000399	0.000232	0.003436	0.000035	0.000320	0.000191	0.001161
Bud Neck	0.006073	0.020762	0.007088	0.014710	0.002258	0.039970	0.004379	0.009721	0.004666	0.031348	0.005756	0.013844
Bud Site	0.002380	0.009014	0.006082	0.021301	0.000827	0.015441	0.005690	0.093542	0.001808	0.012556	0.005890	0.033841
Cell Periphery	0.000176	0.000140	0.000126	0.000269	0.000131	0.000176	0.000138	0.000825	0.000159	0.000160	0.000132	0.000366
Cytoplasm	0.546135*	0.460841*	0.192531*	0.344331*	0.511197*	0.412982*	0.299304*	0.474084*	0.533254*	0.434465*	0.245016*	0.366853*
Cytoplasmic Foci	0.107562	0.174337	0.002402	0.022250	0.083714	0.226377*	0.005617	0.099983	0.098769	0.203018*	0.003982	0.035743
Eisosomes	0.000129	0.000089	0.000016	0.000057	0.000056	0.000167	0.000029	0.000164	0.000102	0.000132	0.000022	0.000076
Endoplasmic Reticulum	0.000581	0.001168	0.002250	0.006280	0.000332	0.000858	0.003357	0.002037	0.000489	0.000997	0.002794	0.005543
Endosome	0.004142	0.018753	0.000673	0.008415	0.001660	0.018491	0.002159	0.014035	0.003227	0.018608	0.001403	0.009391
Golgi	0.000842	0.003507	0.000038	0.010558	0.000865	0.006099	0.000718	0.002139	0.000851	0.004935	0.000372	0.009097
Lipid Particles	0.005365	0.007535	0.000445	0.001574	0.002939	0.008566	0.000798	0.004030	0.004471	0.008103	0.000619	0.002001
Mitochondria	0.001793	0.007375	0.000767	0.004563	0.001133	0.017909	0.001023	0.008109	0.001550	0.013181	0.000893	0.005178
Mitotic Spindle	0.000841	0.001813	0.002740	0.036365	0.000557	0.015175	0.005855	0.012465	0.000736	0.009177	0.004272	0.032216
None	0.011009	0.016956	0.006390	0.008978	0.013278	0.005239	0.009124	0.012723	0.011846	0.010498	0.007734	0.009628
Nuclear Periphery	0.000535	0.000857	0.001866	0.006550	0.000359	0.000526	0.002273	0.000749	0.000470	0.000674	0.002066	0.005543
Nuclear Periphery Foci	0.000280	0.000801	0.000325	0.005787	0.000185	0.000425	0.000909	0.005061	0.000245	0.000594	0.000612	0.005661
Nucleolus	0.001221	0.004218	0.000740	0.000761	0.001311	0.003528	0.000881	0.001011	0.001254	0.003838	0.000809	0.000805
Nucleus	0.284816*	0.210857*	0.763556*	0.363465*	0.369103*	0.156364*	0.625008*	0.155570*	0.315891*	0.180824*	0.695452*	0.327379*
Peroxisomes	0.001306	0.008834	0.000107	0.001566	0.000865	0.019413	0.000216	0.004097	0.001143	0.014664	0.000161	0.002005
Vacuole	0.024416	0.049629	0.011568	0.129060	0.008881	0.049998	0.031939	0.094516	0.018688	0.049832	0.021582	0.123064
Vacuole Periphery	0.000370	0.002292	0.000138	0.012477	0.000300	0.001897	0.000351	0.001702	0.000345	0.002074	0.000243	0.010607

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	-6.90	-10.14	-8.32	-6.44	-6.72	-5.99	-9.56	-2.93	-2.56	-2.65	-8.36	-14.27	-6.67	-5.12	-5.48
Bud Neck	-7.99	-1.79	-3.64	3.94	-2.09	-15.19	-5.39	-2.60	9.43	-1.09	-16.46	-3.32	-4.90	9.93	-3.07
Bud Site	-6.09	-2.87	-5.66	-2.75	-3.68	-10.35	-4.71	-5.30	-4.21	-4.97	-11.09	-4.55	-7.55	-4.04	-6.18
Cell Periphery	3.65	3.52	-0.11	-1.87	-2.38	-3.54	0.67	-4.54	-4.15	-4.59	0.14	3.85	-3.66	-3.68	-4.92
Cytoplasm	8.94	46.25	25.63	16.52	-4.71	8.83	24.23	2.85	-0.41	-5.66	13.80	49.24	22.97	13.84	-4.57
Cytoplasmic Foci	-10.34	31.88	25.62	27.93	-5.69	-21.19	20.64	0.74	12.01	-6.35	-22.17	37.69	20.98	38.16	-7.64
Eisosomes	2.03	5.91	2.34	1.14	-13.86	-12.34	3.88	-3.78	0.93	-4.38	-2.34	6.01	0.61	5.11	-11.24
Endoplasmic Reticulum	-4.75	-13.06	-13.80	-12.65	-9.33	-3.41	-18.86	-5.61	-4.10	5.18	-4.82	-22.24	-14.40	-13.16	-6.79
Endosome	-10.06	9.71	0.10	9.47	-5.12	-14.60	-0.79	-3.27	2.77	-3.14	-16.69	6.80	-2.58	11.79	-5.23
Golgi	-3.49	3.59	-1.17	0.56	-1.74	-5.69	0.66	-0.78	5.24	-1.84	-7.29	2.12	-1.24	1.95	-1.79
Lipid Particles	-2.74	11.17	6.91	7.80	-12.55	-8.22	8.74	-0.14	3.19	-1.42	-6.70	13.02	5.07	10.42	-5.75
Mitochondria	-5.01	1.80	-0.17	5.12	-2.52	-9.52	0.66	-0.95	2.38	-0.99	-10.43	1.88	-1.06	7.60	-1.85
Mitotic Spindle	-1.77	-2.82	-5.74	-5.54	-5.10	-6.91	-4.16	-1.62	-0.19	-1.06	-7.02	-4.93	-5.91	-3.74	-4.86
None	-3.06	4.70	4.26	5.82	-0.02	6.15	4.62	0.49	-0.68	-0.42	1.12	6.36	3.38	2.44	-0.15
Nuclear Periphery	-1.56	-12.39	-5.53	-5.10	-3.54	-2.74	-12.64	-2.44	-1.88	4.90	-1.91	-16.34	-5.76	-5.45	-2.61
Nuclear Periphery Foci	-5.64	-1.89	-4.46	-3.64	-4.26	-2.79	-4.28	-3.86	-3.58	-2.48	-5.46	-4.52	-5.38	-4.75	-4.18
Nucleolus	-7.90	0.21	1.38	8.17	0.75	-4.43	-0.31	1.37	4.60	1.31	-8.02	-0.17	1.96	8.45	1.54
Nucleus	8.05	-59.41	-7.49	-12.11	26.55	21.02	-23.46	6.13	-1.21	15.49	20.73	-56.68	-3.11	-13.51	26.57
Peroxisomes	-7.92	5.03	1.59	8.40	-2.52	-14.62	5.93	-2.05	10.27	-2.77	-16.31	6.61	-0.05	15.74	-3.71
Vacuole	-10.06	5.23	-19.70	-17.28	-20.44	-18.11	-16.71	-7.39	-5.36	-4.52	-17.78	-10.96	-21.14	-18.03	-18.88
Vacuole Periphery	-7.76	4.46	-1.70	-0.51	-1.85	-8.09	0.69	-1.23	0.85	-1.31	-11.05	3.03	-1.83	-0.53	-1.95