ORF
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.96 0.98 0.96 0.88 0.79 0.76 0.66 0.66 0.57 0.59 0.99 0.99 0.99 0.95 0.96 0.95 0.95 0.96 0.94 0.89 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0.05 0.15 0.29 0.26 0.35 0.45 0.55 0.48 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.06 0.05 0.1 0.16 0.1 0.12 0.06 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 2 0 0 2
Bud 0 0 2 0 1 0 6 2 11 7 11 4 0 0 0 0 2 0 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 4 2 1 1 6 2 1 1 4 5 0 1 0 6 4 10 0 0 0 0 0 0
Cytoplasm 109 157 218 215 268 285 328 331 250 267 167 276 270 407 379 171 135 122 103 156 204 55 227 235
Endoplasmic Reticulum 0 1 5 0 0 1 1 0 0 4 0 9 3 3 1 5 0 3 0 0 2 0 0 0
Endosome 0 0 0 0 1 2 0 0 3 0 0 1 0 1 0 1 1 0 0 1 0 1 0 1
Golgi 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 1 0 0 0
Mitochondria 6 2 1 9 13 50 118 113 132 181 160 227 0 0 0 3 0 2 0 0 0 0 0 2
Nucleus 0 0 0 0 3 3 8 8 9 9 3 9 0 1 3 4 0 3 0 0 0 1 0 1
Nuclear Periphery 0 0 0 0 2 2 0 1 0 3 2 5 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 0 0 1 2 4 18 22 43 59 42 34 28 1 4 6 4 9 5 0 0 1 1 0 1
Unique Cell Count 112 160 226 219 279 325 413 433 380 402 293 470 273 411 383 180 140 129 108 163 218 62 234 252
Labelled Cell Count 115 160 233 230 293 362 490 500 467 515 382 566 274 417 389 195 152 145 108 163 218 62 234 252


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 7.4 7.3 6.2 6.5 5.6 5.3 5.4 5.1 4.9 4.4 4.4 7.8 7.9 7.8 10.0 10.4 9.9 6.0 8.0 8.5
Std Deviation (1e-4) 0.7 1.2 2.6 1.4 2.0 1.2 1.3 1.5 1.7 1.7 1.3 0.8 1.2 1.1 1.1 1.8 1.9 1.8 0.7 1.3 1.4
Intensity Change (Log2) – – – -0.23 -0.16 -0.37 -0.46 -0.44 -0.51 -0.58 -0.74 -0.72 0.1 0.11 0.09 0.46 0.52 0.44 -0.28 0.14 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.8
Cytoplasm 1.1 -0.2 -3.6 -5.8 -6.5 -8.7 -8.6 -10.2 -10.3 1.8 2.3 2.1 -0.7 0 -0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.9 6.0 8.7 8.3 9.9 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.2 3.2 4.6 6.0 4.8 5.0 3.4 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygr111w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available