ORF | |
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Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 2 |
Bud | 0 | 0 | 2 | 0 | 1 | 0 | 6 | 2 | 11 | 7 | 11 | 4 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 4 | 2 | 1 | 1 | 6 | 2 | 1 | 1 | 4 | 5 | 0 | 1 | 0 | 6 | 4 | 10 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 109 | 157 | 218 | 215 | 268 | 285 | 328 | 331 | 250 | 267 | 167 | 276 | 270 | 407 | 379 | 171 | 135 | 122 | 103 | 156 | 204 | 55 | 227 | 235 |
Endoplasmic Reticulum | 0 | 1 | 5 | 0 | 0 | 1 | 1 | 0 | 0 | 4 | 0 | 9 | 3 | 3 | 1 | 5 | 0 | 3 | 0 | 0 | 2 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 |
Golgi | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 |
Mitochondria | 6 | 2 | 1 | 9 | 13 | 50 | 118 | 113 | 132 | 181 | 160 | 227 | 0 | 0 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 3 | 3 | 8 | 8 | 9 | 9 | 3 | 9 | 0 | 1 | 3 | 4 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 0 | 3 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 2 | 4 | 18 | 22 | 43 | 59 | 42 | 34 | 28 | 1 | 4 | 6 | 4 | 9 | 5 | 0 | 0 | 1 | 1 | 0 | 1 |
Unique Cell Count | 112 | 160 | 226 | 219 | 279 | 325 | 413 | 433 | 380 | 402 | 293 | 470 | 273 | 411 | 383 | 180 | 140 | 129 | 108 | 163 | 218 | 62 | 234 | 252 |
Labelled Cell Count | 115 | 160 | 233 | 230 | 293 | 362 | 490 | 500 | 467 | 515 | 382 | 566 | 274 | 417 | 389 | 195 | 152 | 145 | 108 | 163 | 218 | 62 | 234 | 252 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 5.9 | 7.4 | 7.3 | 6.2 | 6.5 | 5.6 | 5.3 | 5.4 | 5.1 | 4.9 | 4.4 | 4.4 | 7.8 | 7.9 | 7.8 | 10.0 | 10.4 | 9.9 | 6.0 | 8.0 | 8.5 |
Std Deviation (1e-4) | 0.7 | 1.2 | 2.6 | 1.4 | 2.0 | 1.2 | 1.3 | 1.5 | 1.7 | 1.7 | 1.3 | 0.8 | 1.2 | 1.1 | 1.1 | 1.8 | 1.9 | 1.8 | 0.7 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.23 | -0.16 | -0.37 | -0.46 | -0.44 | -0.51 | -0.58 | -0.74 | -0.72 | 0.1 | 0.11 | 0.09 | 0.46 | 0.52 | 0.44 | -0.28 | 0.14 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 |
Cytoplasm | 1.1 | -0.2 | -3.6 | -5.8 | -6.5 | -8.7 | -8.6 | -10.2 | -10.3 | 1.8 | 2.3 | 2.1 | -0.7 | 0 | -0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 2.9 | 6.0 | 8.7 | 8.3 | 9.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 3.2 | 3.2 | 4.6 | 6.0 | 4.8 | 5.0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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