Standard name | |
---|---|
Human Ortholog | |
Description | Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 3 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 6 | 7 | 4 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 6 | 2 | 0 | 2 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 130 | 470 | 194 | 177 | 74 | 60 | 17 | 16 | 5 | 7 | 3 | 5 | 145 | 166 | 145 | 168 | 151 | 153 | 121 | 447 | 182 | 118 | 138 | 174 |
Endoplasmic Reticulum | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 14 | 7 | 2 | 0 | 1 | 0 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 2 | 5 | 4 | 4 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 8 | 39 | 17 | 21 | 31 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 1 | 0 | 0 | 6 | 72 | 182 | 246 | 155 | 160 | 151 | 173 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 3 | 0 | 3 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 2 | 12 | 29 | 43 | 43 | 36 | 68 | 0 | 1 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 1 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 1 | 40 | 86 | 59 | 55 | 17 | 17 | 9 | 12 | 2 | 6 | 11 | 15 | 8 | 13 | 2 | 8 | 1 | 2 | 2 | 5 |
Unique Cell Count | 130 | 473 | 197 | 179 | 117 | 206 | 268 | 341 | 226 | 221 | 202 | 253 | 147 | 170 | 154 | 190 | 166 | 171 | 126 | 469 | 189 | 130 | 151 | 188 |
Labelled Cell Count | 131 | 481 | 203 | 183 | 123 | 229 | 283 | 368 | 266 | 248 | 224 | 295 | 155 | 177 | 160 | 207 | 177 | 178 | 126 | 469 | 189 | 130 | 151 | 188 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 75.1 | 52.0 | 60.4 | 51.7 | 17.7 | 13.6 | 12.2 | 12.5 | 11.1 | 12.1 | 12.4 | 10.8 | 62.8 | 47.3 | 39.8 | 47.5 | 61.1 | 63.2 | 63.4 | 62.5 | 58.3 |
Std Deviation (1e-4) | 15.6 | 13.1 | 12.2 | 13.9 | 6.9 | 4.1 | 3.6 | 3.0 | 3.1 | 3.2 | 4.0 | 3.4 | 15.9 | 14.6 | 13.1 | 20.3 | 27.0 | 26.7 | 22.0 | 20.4 | 17.7 |
Intensity Change (Log2) | – | – | – | -0.23 | -1.77 | -2.15 | -2.31 | -2.27 | -2.45 | -2.32 | -2.29 | -2.49 | 0.06 | -0.35 | -0.6 | -0.35 | 0.02 | 0.06 | 0.07 | 0.05 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.3 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | -8.5 | -14.4 | -19.7 | -21.5 | -19.8 | -19.5 | -19.4 | -20.4 | 0.1 | -0.6 | -2.2 | -4.0 | -3.3 | -3.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 6.1 | 4.0 | 4.6 | 5.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 9.2 | 14.8 | 16.2 | 14.6 | 15.2 | 15.4 | 14.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 3.0 | 4.2 | 6.5 | 6.5 | 6.2 | 7.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 8.6 | 10.1 | 6.8 | 5.7 | 3.6 | 3.6 | 0 | 2.7 | 0 | 0 | 3.4 | 3.6 | 0 | 3.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 69.9962 | 70.1011 | 69.9466 | 65.1166 | 65.2398 | 66.3326 | 79.5988 | 90.4047 | 87.2962 | 79.3411 | 73.8162 | 82.9511 | 75.6216 | 74.341 | 74.5633 | 66.4911 | 66.7137 | 70.4152 |
Actin | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 |
Bud | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0002 | 0.0001 | 0.0002 | 0.001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0001 |
Bud Neck | 0.0004 | 0.0004 | 0.0003 | 0.0005 | 0.0005 | 0.0013 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0008 | 0.0003 | 0.0004 | 0.0004 | 0.0006 | 0.003 | 0.0008 |
Bud Periphery | 0.0005 | 0.0003 | 0.0002 | 0 | 0 | 0.0003 | 0.0005 | 0.0002 | 0 | 0.0001 | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0006 | 0.0001 | 0 | 0.0003 | 0 | 0.0004 | 0.0002 | 0 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0012 | 0.0016 | 0.0013 | 0.0003 | 0.0002 | 0.0005 | 0.0008 | 0.0024 | 0.0007 | 0.0004 | 0.0015 | 0.0003 | 0.0011 | 0.0019 | 0.0007 | 0.0002 | 0.0012 | 0.0003 |
Cytoplasm | 0.9362 | 0.9701 | 0.9696 | 0.9825 | 0.9818 | 0.9438 | 0.943 | 0.9749 | 0.9722 | 0.948 | 0.8911 | 0.9766 | 0.9606 | 0.9686 | 0.9734 | 0.975 | 0.9318 | 0.969 |
Cytoplasmic Foci | 0.0004 | 0.0004 | 0.0008 | 0.0005 | 0.0004 | 0.0013 | 0.0027 | 0.0003 | 0.0039 | 0.001 | 0.0011 | 0.0007 | 0.0007 | 0.0005 | 0.0004 | 0.001 | 0.0008 | 0.0007 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0181 | 0.0114 | 0.0071 | 0.0012 | 0.0019 | 0.0227 | 0.0051 | 0.0045 | 0.0053 | 0.0021 | 0.0153 | 0.0105 | 0.0119 | 0.0075 | 0.0078 | 0.0035 | 0.0081 | 0.013 |
Endosome | 0.0012 | 0.0006 | 0.0009 | 0.0007 | 0.0007 | 0.0025 | 0.0017 | 0.0002 | 0.001 | 0.0027 | 0.0027 | 0.0011 | 0.0005 | 0.0003 | 0.0005 | 0.0013 | 0.0007 | 0.0013 |
Golgi | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 |
Lipid Particles | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0023 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0004 | 0.0001 |
Mitochondria | 0.0004 | 0.0013 | 0.0003 | 0 | 0 | 0.0013 | 0.0028 | 0.0055 | 0.0002 | 0.0012 | 0.0068 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
None | 0.0003 | 0.0002 | 0.0011 | 0.0001 | 0.0001 | 0.0002 | 0.0066 | 0.0001 | 0.0011 | 0.0004 | 0.0025 | 0.0001 | 0.0013 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 |
Nuclear Periphery | 0.0111 | 0.0016 | 0.0024 | 0.0019 | 0.0024 | 0.0043 | 0.0053 | 0.0007 | 0.0016 | 0.0027 | 0.0079 | 0.0025 | 0.0065 | 0.002 | 0.0029 | 0.0024 | 0.0055 | 0.0038 |
Nucleolus | 0.0027 | 0 | 0.001 | 0 | 0 | 0.0001 | 0.0026 | 0.0001 | 0.0004 | 0.0004 | 0.0075 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0035 | 0 |
Nucleus | 0.0083 | 0.0028 | 0.0028 | 0.0019 | 0.0025 | 0.01 | 0.0087 | 0.0063 | 0.0014 | 0.0021 | 0.0161 | 0.0016 | 0.0043 | 0.0115 | 0.0049 | 0.0029 | 0.0313 | 0.0044 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0019 | 0 | 0.0014 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 |
Vacuole | 0.0155 | 0.0077 | 0.0103 | 0.0088 | 0.0078 | 0.0091 | 0.0115 | 0.0032 | 0.0054 | 0.0228 | 0.0292 | 0.0041 | 0.0098 | 0.0058 | 0.0072 | 0.0109 | 0.0113 | 0.0051 |
Vacuole Periphery | 0.0031 | 0.0006 | 0.0011 | 0.0011 | 0.0012 | 0.0015 | 0.0043 | 0.0002 | 0.0005 | 0.0154 | 0.0147 | 0.001 | 0.001 | 0.0002 | 0.0007 | 0.0008 | 0.0011 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1660.145 | 2103.3684 | 2625.269 | 2072.1353 | 2565.6297 | 2036.895 | 1852.9049 | 1821.8982 | 2346.0534 | 1785.3217 |
Translational Efficiency | 1.9019 | 1.5942 | 1.3177 | 1.8853 | 1.4577 | 1.7154 | 1.7555 | 1.6284 | 1.4297 | 1.7963 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1031 | 478 | 2392 | 1882 | 2437 | 2082 | 1776 | 1555 | 3468 | 2560 | 4168 | 3437 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2008.34 | 3815.60 | 6527.94 | 4514.66 | 3090.88 | 3680.90 | 5304.02 | 6104.22 | 2769.05 | 3706.05 | 6006.42 | 5233.82 |
Standard Deviation | 527.06 | 812.98 | 1282.04 | 1667.75 | 628.10 | 759.84 | 1703.23 | 1816.92 | 777.58 | 771.83 | 1595.53 | 1908.54 |
Intensity Change Log 2 | — | 0.925906 | 1.700624 | 1.168614 | — | 0.252041 | 0.779069 | 0.981789 | — | 0.555941 | 1.214341 | 1.058283 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000173 | 0.000112 | 0.000060 | 0.000585 | 0.000023 | 0.000080 | 0.000199 | 0.000251 | 0.000067 | 0.000086 | 0.000120 | 0.000434 |
Bud Neck | 0.000581 | 0.000679 | 0.000534 | 0.012924 | 0.000166 | 0.000376 | 0.004358 | 0.006229 | 0.000289 | 0.000433 | 0.002163 | 0.009895 |
Bud Site | 0.004698 | 0.003049 | 0.003153 | 0.006065 | 0.001632 | 0.001489 | 0.002194 | 0.003515 | 0.002544 | 0.001780 | 0.002744 | 0.004912 |
Cell Periphery | 0.001297 | 0.001975 | 0.003749 | 0.000596 | 0.000423 | 0.000449 | 0.000582 | 0.001440 | 0.000683 | 0.000734 | 0.002399 | 0.000978 |
Cytoplasm | 0.870640* | 0.875462* | 0.896700* | 0.115622* | 0.943960* | 0.917020* | 0.391426* | 0.437713* | 0.922163* | 0.909261* | 0.681401* | 0.261346* |
Cytoplasmic Foci | 0.005884 | 0.015242 | 0.004256 | 0.005701 | 0.005788 | 0.004527 | 0.002136 | 0.006424 | 0.005816 | 0.006528 | 0.003352 | 0.006028 |
Eisosomes | 0.000002 | 0.000001 | 0.000001 | 0.000026 | 0.000000 | 0.000001 | 0.000004 | 0.000008 | 0.000001 | 0.000001 | 0.000002 | 0.000018 |
Endoplasmic Reticulum | 0.000305 | 0.000091 | 0.000133 | 0.000335 | 0.000147 | 0.000113 | 0.000134 | 0.000201 | 0.000194 | 0.000109 | 0.000133 | 0.000274 |
Endosome | 0.000346 | 0.000453 | 0.000181 | 0.002472 | 0.000177 | 0.000346 | 0.000767 | 0.000262 | 0.000227 | 0.000366 | 0.000430 | 0.001472 |
Golgi | 0.010116 | 0.017417 | 0.007770 | 0.000819 | 0.009316 | 0.004904 | 0.001129 | 0.002849 | 0.009554 | 0.007241 | 0.004940 | 0.001738 |
Lipid Particles | 0.001346 | 0.002659 | 0.002378 | 0.000657 | 0.000344 | 0.000779 | 0.000378 | 0.000957 | 0.000641 | 0.001130 | 0.001526 | 0.000792 |
Mitochondria | 0.000265 | 0.000129 | 0.000171 | 0.002339 | 0.000254 | 0.000114 | 0.001051 | 0.001075 | 0.000257 | 0.000117 | 0.000546 | 0.001767 |
Mitotic Spindle | 0.000496 | 0.004689 | 0.000489 | 0.009538 | 0.000775 | 0.000953 | 0.001058 | 0.002385 | 0.000692 | 0.001650 | 0.000731 | 0.006302 |
None | 0.008580 | 0.004502 | 0.002063 | 0.014252 | 0.002655 | 0.004226 | 0.011767 | 0.007373 | 0.004417 | 0.004278 | 0.006198 | 0.011139 |
Nuclear Periphery | 0.000075 | 0.000172 | 0.000222 | 0.000519 | 0.000048 | 0.000266 | 0.000343 | 0.000311 | 0.000056 | 0.000248 | 0.000273 | 0.000425 |
Nuclear Periphery Foci | 0.003396 | 0.005532 | 0.003173 | 0.000779 | 0.001618 | 0.002293 | 0.000679 | 0.001057 | 0.002147 | 0.002898 | 0.002110 | 0.000905 |
Nucleolus | 0.000347 | 0.000081 | 0.000059 | 0.022077 | 0.000063 | 0.000098 | 0.001373 | 0.007623 | 0.000147 | 0.000095 | 0.000619 | 0.015538 |
Nucleus | 0.067271 | 0.048090 | 0.028958 | 0.797817* | 0.014103 | 0.042772 | 0.563520* | 0.508598* | 0.029910 | 0.043765 | 0.256737* | 0.666966* |
Peroxisomes | 0.000058 | 0.000018 | 0.000015 | 0.001039 | 0.000010 | 0.000010 | 0.000039 | 0.000340 | 0.000024 | 0.000012 | 0.000025 | 0.000723 |
Vacuole | 0.013802 | 0.015380 | 0.033034 | 0.005163 | 0.003835 | 0.015395 | 0.015051 | 0.009579 | 0.006798 | 0.015392 | 0.025371 | 0.007161 |
Vacuole Periphery | 0.010324 | 0.004266 | 0.012903 | 0.000675 | 0.014661 | 0.003790 | 0.001813 | 0.001808 | 0.013372 | 0.003879 | 0.008178 | 0.001188 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.97 | 4.07 | -7.57 | -10.01 | -12.66 | -3.74 | -14.35 | -13.63 | -8.45 | -1.65 | -1.29 | -7.38 | -15.56 | -13.11 | -11.78 |
Bud Neck | -1.32 | -1.58 | -17.58 | -17.27 | -17.38 | -4.13 | -12.87 | -11.26 | -10.93 | -2.12 | -3.58 | -13.11 | -20.82 | -20.43 | -14.01 |
Bud Site | 1.90 | 2.17 | -0.43 | -2.30 | -2.62 | 0.15 | -3.07 | -4.06 | -3.99 | -0.62 | 2.05 | -1.98 | -4.24 | -5.68 | -2.44 |
Cell Periphery | -1.99 | -6.22 | 3.25 | 5.91 | 10.37 | -0.52 | -2.33 | -5.83 | -5.54 | -4.37 | -0.65 | -8.67 | -1.66 | -1.18 | 7.81 |
Cytoplasm | 0.04 | -2.16 | 101.48* | 77.84* | 127.28* | 8.30 | 80.49* | 62.91* | 57.98* | -3.86 | 4.89 | 43.53 | 119.35* | 111.87* | 54.53* |
Cytoplasmic Foci | -4.52 | 1.64 | 1.66 | 5.06 | 0.48 | 1.61 | 3.79 | -2.37 | -3.43 | -4.73 | -1.91 | 3.33 | -0.43 | 1.21 | -2.84 |
Eisosomes | 2.52 | 2.08 | -11.90 | -12.75 | -12.65 | -4.33 | -13.56 | -10.90 | -10.50 | -4.24 | 0.39 | -11.53 | -16.18 | -16.29 | -13.16 |
Endoplasmic Reticulum | 3.43 | 2.46 | 0.10 | -7.95 | -4.81 | 1.16 | -0.94 | -1.00 | -1.56 | -0.68 | 2.98 | 1.46 | -1.76 | -4.57 | -3.30 |
Endosome | -1.41 | 4.11 | -2.03 | -1.71 | -2.38 | -3.10 | -1.25 | -0.79 | 1.75 | 1.08 | -3.02 | -0.93 | -2.29 | -1.59 | -1.60 |
Golgi | -2.50 | 1.50 | 7.21 | 6.37 | 15.59 | 5.29 | 11.44 | 8.10 | 4.21 | -2.26 | 2.58 | 6.67 | 12.15 | 9.22 | 9.77 |
Lipid Particles | -2.74 | -3.41 | 3.28 | 5.53 | 8.22 | -4.99 | -3.18 | -3.96 | -1.34 | -2.53 | -3.91 | -6.70 | -0.78 | 2.85 | 5.71 |
Mitochondria | 2.48 | 1.77 | -8.16 | -8.83 | -8.65 | 3.56 | -4.84 | -7.19 | -8.46 | -0.79 | 4.46 | -4.03 | -10.63 | -11.94 | -7.26 |
Mitotic Spindle | -2.29 | -0.58 | -5.24 | -1.33 | -5.20 | -0.85 | -2.42 | -2.44 | -2.11 | -0.60 | -2.49 | -2.10 | -5.68 | -4.11 | -4.91 |
None | 3.78 | 6.48 | -1.17 | -5.25 | -8.04 | -2.42 | -7.02 | -5.75 | -3.90 | 3.04 | 0.35 | -1.53 | -5.59 | -6.42 | -4.62 |
Nuclear Periphery | -4.03 | -7.31 | -14.17 | -10.48 | -8.61 | -1.20 | -12.31 | -6.82 | -1.42 | 0.25 | -1.32 | -14.78 | -14.05 | -2.40 | -4.82 |
Nuclear Periphery Foci | -2.37 | -0.07 | 6.08 | 5.68 | 7.29 | -2.41 | 3.48 | 1.49 | 3.44 | -1.64 | -2.63 | -0.72 | 5.77 | 6.79 | 5.81 |
Nucleolus | 1.65 | 1.77 | -10.45 | -10.63 | -10.64 | -1.56 | -4.88 | -6.38 | -6.35 | -4.82 | 0.91 | -4.20 | -12.29 | -12.34 | -11.56 |
Nucleus | 2.41 | 6.74 | -96.63* | -95.13* | -121.24* | -12.55 | -75.98* | -61.19* | -56.49* | 3.60 | -6.56 | -42.49 | -113.47* | -108.77* | -53.07* |
Peroxisomes | 1.56 | 1.73 | -4.88 | -5.26 | -5.29 | -0.69 | -4.80 | -2.28 | -2.27 | -2.08 | 1.51 | -0.60 | -5.02 | -5.13 | -4.98 |
Vacuole | -0.68 | -8.91 | 1.58 | 1.81 | 10.59 | -9.93 | -11.13 | -7.89 | 3.23 | 6.67 | -8.10 | -16.65 | -5.21 | 3.84 | 12.50 |
Vacuole Periphery | 3.23 | -1.35 | 6.14 | 3.91 | 11.79 | 7.92 | 10.31 | 10.31 | 3.64 | -0.20 | 8.51 | 4.71 | 12.32 | 5.64 | 11.03 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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