Standard name
Description Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.98 0.99 0.63 0.29 0.06 0.05 0 0 0 0 0.99 0.98 0.94 0.88 0.91 0.89 0.97 0.96 0.97 0.91 0.91 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.17 0.08 0.1 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0.05 0.35 0.68 0.72 0.69 0.72 0.75 0.68 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.09 0.19 0.19 0.18 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.34 0.42 0.22 0.16 0.08 0.08 0 0.05 0 0 0.07 0.08 0.05 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 1 0 0 0 0 1 0 0 1 0 1 0 1 0 0 0 3 0 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 4 1 4 3 0 0 0 0 0 0
Cell Periphery 1 6 7 4 0 1 2 1 0 0 0 0 6 2 0 2 1 3 0 0 1 0 0 0
Cytoplasm 130 470 194 177 74 60 17 16 5 7 3 5 145 166 145 168 151 153 121 447 182 118 138 174
Endoplasmic Reticulum 0 3 1 0 0 0 0 0 0 0 0 0 2 0 1 14 7 2 0 1 0 0 1 1
Endosome 0 0 0 0 0 3 3 4 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 2 5 4 4 0 1 0 0 0 1 1 2 3 4 0 0 0 0 0 0
Mitochondria 0 0 0 0 1 0 4 8 39 17 21 31 0 0 0 2 2 0 0 1 0 0 0 0
Nucleus 0 1 0 0 6 72 182 246 155 160 151 173 0 0 1 0 2 0 0 3 0 3 1 1
Nuclear Periphery 0 0 0 0 0 0 0 4 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 2 12 29 43 43 36 68 0 1 0 2 1 3 0 0 0 1 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 40 86 59 55 17 17 9 12 2 6 11 15 8 13 2 8 1 2 2 5
Unique Cell Count 130 473 197 179 117 206 268 341 226 221 202 253 147 170 154 190 166 171 126 469 189 130 151 188
Labelled Cell Count 131 481 203 183 123 229 283 368 266 248 224 295 155 177 160 207 177 178 126 469 189 130 151 188


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 75.1 52.0 60.4 51.7 17.7 13.6 12.2 12.5 11.1 12.1 12.4 10.8 62.8 47.3 39.8 47.5 61.1 63.2 63.4 62.5 58.3
Std Deviation (1e-4) 15.6 13.1 12.2 13.9 6.9 4.1 3.6 3.0 3.1 3.2 4.0 3.4 15.9 14.6 13.1 20.3 27.0 26.7 22.0 20.4 17.7
Intensity Change (Log2) -0.23 -1.77 -2.15 -2.31 -2.27 -2.45 -2.32 -2.29 -2.49 0.06 -0.35 -0.6 -0.35 0.02 0.06 0.07 0.05 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.8 0 0 0 0 0 0 0 0 0.3 0 0 0 0 0
Cytoplasm 0.3 -8.5 -14.4 -19.7 -21.5 -19.8 -19.5 -19.4 -20.4 0.1 -0.6 -2.2 -4.0 -3.3 -3.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.5 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 6.1 4.0 4.6 5.1 0 0 0 0 0 0
Nucleus 0 0 9.2 14.8 16.2 14.6 15.2 15.4 14.8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 3.0 4.2 6.5 6.5 6.2 7.9 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 8.6 10.1 6.8 5.7 3.6 3.6 0 2.7 0 0 3.4 3.6 0 3.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps23a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available