Standard name
Human Ortholog
Description Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.98 0.99 0.63 0.29 0.06 0.05 0 0 0 0 0.99 0.98 0.94 0.88 0.91 0.89 0.97 0.96 0.97 0.91 0.91 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.17 0.08 0.1 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0.05 0.35 0.68 0.72 0.69 0.72 0.75 0.68 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.09 0.19 0.19 0.18 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.34 0.42 0.22 0.16 0.08 0.08 0 0.05 0 0 0.07 0.08 0.05 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 1 0 0 0 0 1 0 0 1 0 1 0 1 0 0 0 3 0 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 4 1 4 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 6 7 4 0 1 2 1 0 0 0 0 6 2 0 2 1 3 0 0 1 0 0 0
Cytoplasm 130 470 194 177 74 60 17 16 5 7 3 5 145 166 145 168 151 153 121 447 182 118 138 174
Endoplasmic Reticulum 0 3 1 0 0 0 0 0 0 0 0 0 2 0 1 14 7 2 0 1 0 0 1 1
Endosome 0 0 0 0 0 3 3 4 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 2 5 4 4 0 1 0 0 0 1 1 2 3 4 0 0 0 0 0 0
Mitochondria 0 0 0 0 1 0 4 8 39 17 21 31 0 0 0 2 2 0 0 1 0 0 0 0
Nucleus 0 1 0 0 6 72 182 246 155 160 151 173 0 0 1 0 2 0 0 3 0 3 1 1
Nuclear Periphery 0 0 0 0 0 0 0 4 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 2 12 29 43 43 36 68 0 1 0 2 1 3 0 0 0 1 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 40 86 59 55 17 17 9 12 2 6 11 15 8 13 2 8 1 2 2 5
Unique Cell Count 130 473 197 179 117 206 268 341 226 221 202 253 147 170 154 190 166 171 126 469 189 130 151 188
Labelled Cell Count 131 481 203 183 123 229 283 368 266 248 224 295 155 177 160 207 177 178 126 469 189 130 151 188


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 75.1 52.0 60.4 51.7 17.7 13.6 12.2 12.5 11.1 12.1 12.4 10.8 62.8 47.3 39.8 47.5 61.1 63.2 63.4 62.5 58.3
Std Deviation (1e-4) 15.6 13.1 12.2 13.9 6.9 4.1 3.6 3.0 3.1 3.2 4.0 3.4 15.9 14.6 13.1 20.3 27.0 26.7 22.0 20.4 17.7
Intensity Change (Log2) – – – -0.23 -1.77 -2.15 -2.31 -2.27 -2.45 -2.32 -2.29 -2.49 0.06 -0.35 -0.6 -0.35 0.02 0.06 0.07 0.05 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.8 0 0 0 0 0 0 0 0 0.3 0 0 0 0 0
Cytoplasm 0.3 -8.5 -14.4 -19.7 -21.5 -19.8 -19.5 -19.4 -20.4 0.1 -0.6 -2.2 -4.0 -3.3 -3.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.5 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 6.1 4.0 4.6 5.1 0 0 0 0 0 0
Nucleus 0 0 9.2 14.8 16.2 14.6 15.2 15.4 14.8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 3.0 4.2 6.5 6.5 6.2 7.9 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 8.6 10.1 6.8 5.7 3.6 3.6 0 2.7 0 0 3.4 3.6 0 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 69.9962 70.1011 69.9466 65.1166 65.2398 66.3326 79.5988 90.4047 87.2962 79.3411 73.8162 82.9511 75.6216 74.341 74.5633 66.4911 66.7137 70.4152
Actin 0.0001 0.0002 0.0002 0.0001 0.0001 0.0003 0.0005 0.0001 0.001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0004 0.0001
Bud 0.0002 0.0003 0.0002 0.0002 0.0002 0.0005 0.0006 0.0002 0.0001 0.0002 0.001 0.0002 0.0002 0.0002 0.0001 0.0003 0.0002 0.0001
Bud Neck 0.0004 0.0004 0.0003 0.0005 0.0005 0.0013 0.0003 0.0002 0.0002 0.0002 0.0003 0.0008 0.0003 0.0004 0.0004 0.0006 0.003 0.0008
Bud Periphery 0.0005 0.0003 0.0002 0 0 0.0003 0.0005 0.0002 0 0.0001 0.0011 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0
Bud Site 0.0001 0.0003 0.0001 0 0 0 0.0002 0.0006 0.0001 0 0.0003 0 0.0004 0.0002 0 0 0.0001 0
Cell Periphery 0.0012 0.0016 0.0013 0.0003 0.0002 0.0005 0.0008 0.0024 0.0007 0.0004 0.0015 0.0003 0.0011 0.0019 0.0007 0.0002 0.0012 0.0003
Cytoplasm 0.9362 0.9701 0.9696 0.9825 0.9818 0.9438 0.943 0.9749 0.9722 0.948 0.8911 0.9766 0.9606 0.9686 0.9734 0.975 0.9318 0.969
Cytoplasmic Foci 0.0004 0.0004 0.0008 0.0005 0.0004 0.0013 0.0027 0.0003 0.0039 0.001 0.0011 0.0007 0.0007 0.0005 0.0004 0.001 0.0008 0.0007
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0181 0.0114 0.0071 0.0012 0.0019 0.0227 0.0051 0.0045 0.0053 0.0021 0.0153 0.0105 0.0119 0.0075 0.0078 0.0035 0.0081 0.013
Endosome 0.0012 0.0006 0.0009 0.0007 0.0007 0.0025 0.0017 0.0002 0.001 0.0027 0.0027 0.0011 0.0005 0.0003 0.0005 0.0013 0.0007 0.0013
Golgi 0.0001 0.0002 0.0001 0 0 0.0003 0.0002 0.0001 0.0004 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0 0.0001
Lipid Particles 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0005 0.0002 0.0023 0.0002 0.0003 0.0001 0.0002 0.0004 0.0002 0.0001 0.0004 0.0001
Mitochondria 0.0004 0.0013 0.0003 0 0 0.0013 0.0028 0.0055 0.0002 0.0012 0.0068 0.0001 0.0002 0 0.0003 0.0002 0.0001 0.0001
None 0.0003 0.0002 0.0011 0.0001 0.0001 0.0002 0.0066 0.0001 0.0011 0.0004 0.0025 0.0001 0.0013 0.0001 0.0001 0.0002 0.0003 0.0001
Nuclear Periphery 0.0111 0.0016 0.0024 0.0019 0.0024 0.0043 0.0053 0.0007 0.0016 0.0027 0.0079 0.0025 0.0065 0.002 0.0029 0.0024 0.0055 0.0038
Nucleolus 0.0027 0 0.001 0 0 0.0001 0.0026 0.0001 0.0004 0.0004 0.0075 0 0.0002 0.0001 0 0 0.0035 0
Nucleus 0.0083 0.0028 0.0028 0.0019 0.0025 0.01 0.0087 0.0063 0.0014 0.0021 0.0161 0.0016 0.0043 0.0115 0.0049 0.0029 0.0313 0.0044
Peroxisomes 0 0 0 0 0 0 0.0002 0 0.0008 0 0.0001 0 0 0 0 0 0 0
Punctate Nuclear 0.0001 0 0 0 0 0 0.0019 0 0.0014 0 0.0002 0 0.0004 0 0 0 0.0001 0
Vacuole 0.0155 0.0077 0.0103 0.0088 0.0078 0.0091 0.0115 0.0032 0.0054 0.0228 0.0292 0.0041 0.0098 0.0058 0.0072 0.0109 0.0113 0.0051
Vacuole Periphery 0.0031 0.0006 0.0011 0.0011 0.0012 0.0015 0.0043 0.0002 0.0005 0.0154 0.0147 0.001 0.001 0.0002 0.0007 0.0008 0.0011 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1660.145 2103.3684 2625.269 2072.1353 2565.6297 2036.895 1852.9049 1821.8982 2346.0534 1785.3217
Translational Efficiency 1.9019 1.5942 1.3177 1.8853 1.4577 1.7154 1.7555 1.6284 1.4297 1.7963

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
Localization
Cell Percentages cytoplasm (95%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rps23a

Rps23a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps23a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available