Standard name | |
---|---|
Human Ortholog | |
Description | FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 4 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 4 | 5 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 9 | 11 | 22 | 12 | 10 | 12 | 6 | 6 | 5 | 5 | 1 | 15 | 22 | 9 | 11 | 7 | 0 | 0 | 1 | 1 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 2 | 3 | 5 | 1 | 0 | 5 | 2 | 3 | 8 | 9 | 5 | 0 | 0 | 0 | 0 | 1 | 3 |
Endosome | 1 | 0 | 4 | 1 | 9 | 0 | 6 | 8 | 3 | 0 | 1 | 2 | 3 | 1 | 1 | 5 | 4 | 7 | 0 | 0 | 4 | 3 | 3 | 15 |
Golgi | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 2 |
Mitochondria | 7 | 8 | 30 | 35 | 25 | 78 | 83 | 111 | 82 | 131 | 134 | 129 | 2 | 0 | 0 | 8 | 19 | 16 | 1 | 0 | 0 | 7 | 18 | 23 |
Nucleus | 5 | 4 | 7 | 11 | 14 | 5 | 17 | 25 | 12 | 19 | 20 | 11 | 6 | 12 | 6 | 23 | 23 | 9 | 2 | 1 | 1 | 0 | 5 | 4 |
Nuclear Periphery | 110 | 42 | 193 | 209 | 219 | 247 | 252 | 352 | 249 | 246 | 228 | 196 | 102 | 238 | 184 | 125 | 144 | 114 | 156 | 48 | 207 | 339 | 404 | 395 |
Nucleolus | 24 | 8 | 59 | 56 | 79 | 117 | 147 | 233 | 147 | 172 | 158 | 151 | 13 | 26 | 17 | 25 | 20 | 32 | 6 | 4 | 2 | 12 | 12 | 13 |
Peroxisomes | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
SpindlePole | 0 | 1 | 2 | 0 | 1 | 0 | 3 | 0 | 1 | 1 | 0 | 1 | 0 | 6 | 9 | 0 | 4 | 1 | 0 | 0 | 2 | 2 | 3 | 2 |
Vac/Vac Membrane | 14 | 8 | 16 | 17 | 29 | 19 | 13 | 23 | 22 | 12 | 13 | 10 | 8 | 37 | 30 | 44 | 54 | 37 | 6 | 7 | 2 | 4 | 18 | 24 |
Unique Cell Count | 131 | 56 | 226 | 254 | 306 | 344 | 380 | 575 | 357 | 417 | 372 | 337 | 120 | 284 | 238 | 195 | 220 | 167 | 177 | 65 | 230 | 383 | 481 | 500 |
Labelled Cell Count | 161 | 72 | 323 | 341 | 404 | 479 | 538 | 770 | 527 | 594 | 563 | 508 | 140 | 339 | 281 | 248 | 289 | 233 | 177 | 65 | 230 | 383 | 481 | 500 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.6 | 13.4 | 12.3 | 12.4 | 12.5 | 10.5 | 10.4 | 10.6 | 10.7 | 10.0 | 9.9 | 9.8 | 15.7 | 14.4 | 15.1 | 10.6 | 12.0 | 15.7 | 16.5 | 16.0 | 17.3 |
Std Deviation (1e-4) | 3.2 | 4.1 | 4.0 | 3.6 | 3.7 | 2.9 | 3.6 | 3.3 | 3.6 | 4.3 | 3.7 | 3.0 | 6.3 | 4.8 | 6.2 | 6.4 | 7.1 | 9.0 | 5.6 | 4.8 | 5.9 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.02 | -0.22 | -0.24 | -0.21 | -0.19 | -0.29 | -0.3 | -0.33 | 0.36 | 0.23 | 0.3 | -0.21 | -0.03 | 0.36 | 0.43 | 0.38 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | 1.6 | -0.3 | -0.9 | -1.5 | -1.7 | -2.0 | -2.1 | -1.9 | 0 | 0.7 | 2.3 | 0.3 | 0.5 | 0.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.2 | -1.9 | 2.8 | 2.6 | 2.0 | 2.9 | 5.1 | 6.0 | 6.5 | -3.5 | -6.3 | -5.8 | -3.3 | -1.6 | -1.1 |
Nucleus | 0.7 | 0.9 | 0 | 0.8 | 0.8 | 0.2 | 0.9 | 1.3 | 0.1 | 0 | 0.7 | -0.4 | 3.5 | 3.1 | 1.1 |
Nuclear Periphery | -0.9 | -3.8 | -3.8 | -5.1 | -6.6 | -4.3 | -6.9 | -6.3 | -6.9 | -0.1 | -0.5 | -2.2 | -5.1 | -4.9 | -4.1 |
Nucleolus | -1.0 | -0.1 | 2.0 | 3.2 | 3.8 | 3.7 | 3.8 | 4.0 | 4.5 | -3.3 | -5.1 | -5.5 | -3.4 | -4.7 | -1.6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 |
Vacuole | -0.2 | 1.0 | -0.8 | -2.0 | -1.8 | -0.4 | -2.5 | -2.0 | -2.3 | -0.1 | 2.2 | 2.0 | 4.5 | 5.1 | 4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.6993 | 13.9608 | 13.1094 | 11.8437 | 12.254 | 12.4996 | 10.6253 | 16.1353 | 14.5771 | 13.5446 | 12.42 | 13.7767 | 11.2872 | 15.7136 | 12.3259 | 12.201 | 13.2978 | 11.7674 |
Actin | 0.0142 | 0.0001 | 0.0003 | 0.0002 | 0.0077 | 0.0001 | 0.0123 | 0.0001 | 0.0008 | 0.0051 | 0.0201 | 0 | 0.1224 | 0.0001 | 0.002 | 0.0024 | 0.0132 | 0.0001 |
Bud | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0003 | 0.0006 | 0 | 0.0008 | 0 | 0.0011 | 0.0001 | 0.0003 | 0.0001 |
Bud Neck | 0.0072 | 0 | 0 | 0 | 0.0002 | 0 | 0.0095 | 0 | 0 | 0.0001 | 0.0006 | 0 | 0.0101 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0006 |
Bud Periphery | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0011 | 0.0007 | 0 | 0.0026 | 0.0002 | 0.0013 | 0.0007 | 0.0023 | 0.0001 |
Bud Site | 0.0062 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0009 | 0 | 0 | 0.0002 | 0.0037 | 0 | 0.0115 | 0 | 0.0015 | 0.0001 | 0.0005 | 0 |
Cell Periphery | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0473 | 0 | 0.0003 | 0.0001 | 0.056 | 0 |
Cytoplasm | 0.0025 | 0.0027 | 0.002 | 0 | 0.0007 | 0 | 0.0015 | 0 | 0 | 0.0009 | 0.0042 | 0 | 0.0006 | 0 | 0.0132 | 0.0001 | 0.0005 | 0.0015 |
Cytoplasmic Foci | 0.0064 | 0.0001 | 0.0008 | 0 | 0.0018 | 0.0001 | 0.0127 | 0 | 0.0005 | 0.0006 | 0.0092 | 0 | 0.0046 | 0 | 0.0062 | 0.0002 | 0.0132 | 0.0001 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0043 | 0.0004 | 0.0004 | 0.0006 | 0.0012 | 0.0001 | 0.0016 | 0.0003 | 0.0004 | 0.0032 | 0.0043 | 0.0001 | 0.0018 | 0.0003 | 0.0012 | 0.001 | 0.0014 | 0.0001 |
Endosome | 0.0083 | 0.0002 | 0.0006 | 0.0001 | 0.0088 | 0.0001 | 0.0089 | 0.0001 | 0.0003 | 0.01 | 0.0259 | 0 | 0.0039 | 0.0001 | 0.0014 | 0.0003 | 0.0188 | 0.0001 |
Golgi | 0.0026 | 0 | 0.0001 | 0.0001 | 0.0019 | 0.0001 | 0.0047 | 0.0001 | 0.0006 | 0.0011 | 0.0135 | 0 | 0.0059 | 0.0001 | 0.0021 | 0.0008 | 0.015 | 0.0001 |
Lipid Particles | 0.0066 | 0.0003 | 0.0005 | 0.0008 | 0.004 | 0.0059 | 0.0056 | 0.0015 | 0.0191 | 0.0031 | 0.0084 | 0.0004 | 0.0075 | 0.0006 | 0.0082 | 0.0186 | 0.0984 | 0.0008 |
Mitochondria | 0.0034 | 0.0008 | 0.0005 | 0.0007 | 0.0017 | 0.0043 | 0.0008 | 0.0034 | 0.0022 | 0.0116 | 0.0043 | 0.0001 | 0.007 | 0.0012 | 0.0153 | 0.0026 | 0.0059 | 0.0002 |
None | 0.0049 | 0.0002 | 0.007 | 0.0001 | 0.0026 | 0 | 0.0009 | 0.0001 | 0.0003 | 0.0025 | 0.0006 | 0 | 0.003 | 0.0001 | 0.0006 | 0.0005 | 0.0008 | 0.0005 |
Nuclear Periphery | 0.8767 | 0.9432 | 0.9508 | 0.9646 | 0.9192 | 0.968 | 0.8911 | 0.9181 | 0.9126 | 0.859 | 0.8617 | 0.981 | 0.7082 | 0.94 | 0.8593 | 0.9102 | 0.6447 | 0.9526 |
Nucleolus | 0.0095 | 0.0006 | 0.0004 | 0.0003 | 0.0013 | 0.0035 | 0.0007 | 0.0025 | 0.0014 | 0.0116 | 0.0004 | 0.0001 | 0.0014 | 0.0003 | 0.0009 | 0.0016 | 0.001 | 0.0013 |
Nucleus | 0.011 | 0.024 | 0.004 | 0.003 | 0.0093 | 0.0047 | 0.0015 | 0.0191 | 0.0031 | 0.0185 | 0.0011 | 0.0007 | 0.0043 | 0.002 | 0.0049 | 0.0041 | 0.0016 | 0.0223 |
Peroxisomes | 0.002 | 0 | 0.0001 | 0 | 0.0008 | 0.0002 | 0.0013 | 0 | 0.0003 | 0.0004 | 0.002 | 0 | 0.0053 | 0 | 0.0101 | 0.0002 | 0.0684 | 0 |
Punctate Nuclear | 0.0122 | 0.0004 | 0.0021 | 0.0004 | 0.0153 | 0.0006 | 0.0091 | 0.0004 | 0.0007 | 0.004 | 0.0014 | 0.0002 | 0.0026 | 0.0002 | 0.0016 | 0.0019 | 0.0021 | 0.0025 |
Vacuole | 0.001 | 0.0008 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0005 | 0.0004 | 0.0006 | 0.0017 | 0.0009 | 0.0001 | 0.0091 | 0.0004 | 0.0012 | 0.0006 | 0.0221 | 0.0001 |
Vacuole Periphery | 0.0196 | 0.0261 | 0.0301 | 0.0287 | 0.0224 | 0.0119 | 0.0356 | 0.0537 | 0.0566 | 0.0647 | 0.0359 | 0.0172 | 0.039 | 0.0545 | 0.0674 | 0.0537 | 0.0335 | 0.017 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 35.2872 | 43.6772 | 34.2109 | 46.2429 | 49.7107 | 24.4161 | 45.8487 | 45.3305 | 43.4346 | 44.3396 |
Translational Efficiency | 2.0706 | 1.9969 | 1.9289 | 1.2892 | 1.5011 | 2.9953 | 1.5054 | 1.6149 | 1.5998 | 1.582 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
66 | 275 | 1070 | 85 | 207 | 1656 | 172 | 624 | 273 | 1931 | 1242 | 709 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1788.40 | 1558.04 | 1319.09 | 1804.95 | 1441.42 | 1354.89 | 1665.13 | 1513.14 | 1525.31 | 1383.82 | 1367.01 | 1548.12 |
Standard Deviation | 245.61 | 312.58 | 341.51 | 268.21 | 246.56 | 248.58 | 246.10 | 322.79 | 287.66 | 268.23 | 350.93 | 330.62 |
Intensity Change Log 2 | — | -0.198937 | -0.439126 | 0.013289 | — | -0.089315 | 0.208144 | 0.070055 | — | -0.148983 | -0.114100 | 0.038899 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000915 | 0.000666 | 0.001295 | 0.000394 | 0.000317 | 0.000783 | 0.000259 | 0.001238 | 0.000462 | 0.000767 | 0.001152 | 0.001137 |
Bud Neck | 0.018047 | 0.000389 | 0.001866 | 0.002551 | 0.000378 | 0.000497 | 0.000634 | 0.005031 | 0.004650 | 0.000481 | 0.001695 | 0.004734 |
Bud Site | 0.001945 | 0.000020 | 0.000254 | 0.000216 | 0.000043 | 0.000167 | 0.000027 | 0.001924 | 0.000503 | 0.000147 | 0.000222 | 0.001719 |
Cell Periphery | 0.000150 | 0.000041 | 0.000132 | 0.000045 | 0.000011 | 0.000052 | 0.000012 | 0.000105 | 0.000044 | 0.000050 | 0.000116 | 0.000098 |
Cytoplasm | 0.000322 | 0.000062 | 0.000719 | 0.000095 | 0.000036 | 0.000248 | 0.000065 | 0.000713 | 0.000105 | 0.000221 | 0.000629 | 0.000639 |
Cytoplasmic Foci | 0.008819 | 0.000919 | 0.001052 | 0.003408 | 0.000648 | 0.001184 | 0.001117 | 0.013271 | 0.002623 | 0.001146 | 0.001061 | 0.012088 |
Eisosomes | 0.000042 | 0.000014 | 0.000024 | 0.000015 | 0.000009 | 0.000020 | 0.000007 | 0.000042 | 0.000017 | 0.000019 | 0.000022 | 0.000039 |
Endoplasmic Reticulum | 0.003776 | 0.000882 | 0.002056 | 0.000657 | 0.001220 | 0.001854 | 0.000739 | 0.002028 | 0.001838 | 0.001716 | 0.001873 | 0.001864 |
Endosome | 0.004646 | 0.000122 | 0.000798 | 0.000563 | 0.000091 | 0.000568 | 0.000119 | 0.007049 | 0.001192 | 0.000505 | 0.000704 | 0.006271 |
Golgi | 0.002960 | 0.000155 | 0.000945 | 0.001141 | 0.000092 | 0.000440 | 0.000257 | 0.008059 | 0.000785 | 0.000399 | 0.000850 | 0.007230 |
Lipid Particles | 0.001996 | 0.000592 | 0.000793 | 0.009761 | 0.000346 | 0.001905 | 0.000910 | 0.015829 | 0.000745 | 0.001718 | 0.000809 | 0.015102 |
Mitochondria | 0.020567 | 0.005200 | 0.004709 | 0.010904 | 0.001200 | 0.005578 | 0.003524 | 0.014400 | 0.005882 | 0.005524 | 0.004545 | 0.013981 |
Mitotic Spindle | 0.140898* | 0.000177 | 0.003276 | 0.033420 | 0.004060 | 0.002734 | 0.001100 | 0.061264 | 0.037141 | 0.002369 | 0.002975 | 0.057926 |
None | 0.000346 | 0.000654 | 0.003186 | 0.000709 | 0.000156 | 0.001192 | 0.000120 | 0.001114 | 0.000202 | 0.001115 | 0.002761 | 0.001065 |
Nuclear Periphery | 0.552512* | 0.771678* | 0.523659* | 0.674040* | 0.790418* | 0.730526* | 0.764165* | 0.524542* | 0.732902* | 0.736387* | 0.556966* | 0.542465* |
Nuclear Periphery Foci | 0.145344 | 0.151558 | 0.198568* | 0.217259* | 0.147764 | 0.162132 | 0.185063 | 0.256537* | 0.147179 | 0.160626 | 0.196698* | 0.251828* |
Nucleolus | 0.046885 | 0.027278 | 0.134533 | 0.012666 | 0.028342 | 0.045195 | 0.024573 | 0.039035 | 0.032825 | 0.042643 | 0.119305 | 0.035873 |
Nucleus | 0.009631 | 0.013600 | 0.097972 | 0.002778 | 0.015887 | 0.023128 | 0.009225 | 0.017803 | 0.014374 | 0.021771 | 0.085682 | 0.016002 |
Peroxisomes | 0.002501 | 0.000741 | 0.002036 | 0.000530 | 0.001611 | 0.001727 | 0.000628 | 0.002542 | 0.001826 | 0.001587 | 0.001841 | 0.002301 |
Vacuole | 0.015393 | 0.002735 | 0.004863 | 0.003606 | 0.001414 | 0.004043 | 0.001266 | 0.008729 | 0.004794 | 0.003857 | 0.004365 | 0.008115 |
Vacuole Periphery | 0.022306 | 0.022516 | 0.017263 | 0.025241 | 0.005957 | 0.016028 | 0.006191 | 0.018743 | 0.009909 | 0.016952 | 0.015730 | 0.019522 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.12 | -1.15 | 2.09 | 1.19 | 4.14 | -4.96 | 0.69 | -2.49 | -1.26 | -2.66 | -2.95 | -4.45 | -2.01 | -1.16 | -0.01 |
Bud Neck | 2.82 | 2.61 | 2.48 | -2.12 | -0.69 | -0.88 | -1.25 | -5.18 | -5.07 | -4.89 | 2.65 | 1.97 | 0.08 | -5.32 | -3.87 |
Bud Site | 3.07 | 2.74 | 2.80 | -2.29 | 0.43 | -2.00 | 0.79 | -2.98 | -2.77 | -3.00 | 2.23 | 1.84 | -1.97 | -2.81 | -2.68 |
Cell Periphery | 2.71 | 0.79 | 2.68 | -0.19 | 2.39 | -3.15 | -0.23 | -7.03 | -3.08 | -6.98 | 0.07 | -2.03 | -2.89 | -3.11 | 0.42 |
Cytoplasm | 1.28 | -0.37 | 1.20 | -1.10 | 2.16 | -2.62 | -0.88 | -2.81 | -1.99 | -2.71 | -0.40 | -1.76 | -2.13 | -2.03 | -0.13 |
Cytoplasmic Foci | 2.63 | 2.58 | 1.85 | -2.82 | -2.61 | -2.26 | -1.51 | -6.24 | -5.97 | -5.99 | 1.97 | 2.04 | -4.70 | -6.12 | -6.15 |
Eisosomes | 2.26 | 1.38 | 2.04 | -0.28 | 1.28 | -4.01 | 0.55 | -4.55 | -3.07 | -4.86 | -0.58 | -0.82 | -3.05 | -3.10 | -2.29 |
Endoplasmic Reticulum | 2.01 | 1.19 | 2.16 | 1.13 | 6.38 | -2.21 | 2.09 | -2.16 | -0.61 | -3.51 | 0.28 | -0.10 | -0.22 | -0.60 | -0.20 |
Endosome | 2.05 | 1.74 | 1.84 | -1.82 | 0.76 | -4.19 | -0.82 | -5.02 | -4.66 | -5.00 | 1.24 | 0.85 | -3.78 | -4.70 | -4.50 |
Golgi | 2.81 | 2.05 | 1.91 | -3.47 | -0.42 | -1.82 | -2.97 | -3.52 | -3.35 | -3.44 | 1.47 | 0.06 | -3.13 | -3.41 | -3.16 |
Lipid Particles | 2.72 | 2.32 | -1.51 | -1.82 | -1.78 | -3.33 | -1.05 | -5.97 | -5.28 | -5.64 | -2.23 | -0.20 | -6.06 | -5.58 | -6.04 |
Mitochondria | 1.49 | 1.57 | 0.82 | -0.94 | -1.08 | -6.19 | -0.85 | -4.94 | -3.20 | -2.84 | 0.12 | 0.51 | -2.35 | -3.33 | -3.74 |
Mitotic Spindle | 4.21 | 4.11 | 2.88 | -2.09 | -1.89 | 0.43 | 1.01 | -7.80 | -8.54 | -8.85 | 3.82 | 3.75 | -2.06 | -8.78 | -8.70 |
None | -1.01 | -3.33 | -0.74 | -0.13 | 2.75 | -2.91 | 0.99 | -3.03 | 0.13 | -3.15 | -2.90 | -3.58 | -2.99 | 0.08 | 2.19 |
Nuclear Periphery | -5.17 | 0.35 | -2.67 | 3.65 | -5.60 | 4.17 | 1.39 | 15.43 | 16.50 | 14.00 | -0.41 | 9.43 | 10.01 | 16.76 | 1.44 |
Nuclear Periphery Foci | -0.43 | -3.27 | -3.19 | -3.69 | -1.08 | -1.76 | -3.12 | -10.09 | -11.73 | -5.86 | -1.86 | -6.00 | -10.66 | -12.32 | -6.50 |
Nucleolus | 1.41 | -4.93 | 2.33 | 2.31 | 16.38 | -2.59 | 0.46 | -1.53 | 1.04 | -1.99 | -1.38 | -10.58 | -0.37 | 1.31 | 12.07 |
Nucleus | -0.34 | -12.94 | 2.52 | 2.68 | 16.62 | -1.40 | 1.21 | -1.07 | 0.31 | -2.88 | -1.65 | -11.51 | -1.03 | 0.62 | 11.55 |
Peroxisomes | 2.55 | 0.79 | 2.77 | 0.95 | 6.37 | -0.16 | 1.61 | -1.25 | -1.52 | -3.11 | 0.56 | 0.06 | -0.82 | -1.52 | -1.09 |
Vacuole | 2.69 | 2.28 | 2.51 | -0.96 | 1.30 | -3.69 | 0.23 | -6.20 | -4.03 | -6.53 | 0.95 | 0.56 | -1.79 | -4.14 | -3.51 |
Vacuole Periphery | -0.02 | 0.52 | -0.26 | -0.44 | -1.36 | -5.23 | -0.14 | -5.25 | -1.14 | -6.25 | -2.48 | -1.95 | -2.98 | -1.15 | -1.60 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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