Standard name
Description FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0.05 0.09 0.05 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.14 0.13 0.14 0.08 0.23 0.22 0.19 0.23 0.31 0.36 0.38 0 0 0 0 0.09 0.1 0 0 0 0 0 0.05
Nucleus 0 0.07 0 0 0.05 0 0 0 0 0.05 0.05 0 0.05 0 0 0.12 0.1 0.05 0 0 0 0 0 0
Nuclear Periphery 0.84 0.75 0.85 0.82 0.72 0.72 0.66 0.61 0.7 0.59 0.61 0.58 0.85 0.84 0.77 0.64 0.65 0.68 0.89 0.75 0.9 0.89 0.84 0.79
Nucleolus 0.18 0.14 0.26 0.22 0.26 0.34 0.39 0.41 0.41 0.41 0.42 0.45 0.11 0.09 0.07 0.13 0.09 0.19 0 0.06 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.14 0.07 0.07 0.09 0.06 0 0 0.06 0 0 0 0.07 0.13 0.13 0.23 0.25 0.22 0 0.11 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 2 0 0 0 1 1 0 0 0 0 2 1 1 0 0 0 0 3 1 4
Bud 0 0 0 0 0 0 0 2 0 1 3 0 0 0 0 0 0 1 1 2 1 4 5 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 1 0 0 0 0 2 1 0 0 0 2 0 2 2 0 0 0
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 3 0 0 1 0 0 0 0 0 0
Cytoplasm 0 0 9 11 22 12 10 12 6 6 5 5 1 15 22 9 11 7 0 0 1 1 0 1
Endoplasmic Reticulum 0 0 1 0 1 1 3 2 3 5 1 0 5 2 3 8 9 5 0 0 0 0 1 3
Endosome 1 0 4 1 9 0 6 8 3 0 1 2 3 1 1 5 4 7 0 0 4 3 3 15
Golgi 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 2 2
Mitochondria 7 8 30 35 25 78 83 111 82 131 134 129 2 0 0 8 19 16 1 0 0 7 18 23
Nucleus 5 4 7 11 14 5 17 25 12 19 20 11 6 12 6 23 23 9 2 1 1 0 5 4
Nuclear Periphery 110 42 193 209 219 247 252 352 249 246 228 196 102 238 184 125 144 114 156 48 207 339 404 395
Nucleolus 24 8 59 56 79 117 147 233 147 172 158 151 13 26 17 25 20 32 6 4 2 12 12 13
Peroxisomes 0 0 1 0 1 0 1 1 1 0 0 1 0 0 2 0 0 1 0 0 0 0 1 2
SpindlePole 0 1 2 0 1 0 3 0 1 1 0 1 0 6 9 0 4 1 0 0 2 2 3 2
Vac/Vac Membrane 14 8 16 17 29 19 13 23 22 12 13 10 8 37 30 44 54 37 6 7 2 4 18 24
Unique Cell Count 131 56 226 254 306 344 380 575 357 417 372 337 120 284 238 195 220 167 177 65 230 383 481 500
Labelled Cell Count 161 72 323 341 404 479 538 770 527 594 563 508 140 339 281 248 289 233 177 65 230 383 481 500


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.6 13.4 12.3 12.4 12.5 10.5 10.4 10.6 10.7 10.0 9.9 9.8 15.7 14.4 15.1 10.6 12.0 15.7 16.5 16.0 17.3
Std Deviation (1e-4) 3.2 4.1 4.0 3.6 3.7 2.9 3.6 3.3 3.6 4.3 3.7 3.0 6.3 4.8 6.2 6.4 7.1 9.0 5.6 4.8 5.9
Intensity Change (Log2) 0.02 0.02 -0.22 -0.24 -0.21 -0.19 -0.29 -0.3 -0.33 0.36 0.23 0.3 -0.21 -0.03 0.36 0.43 0.38 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 1.6 -0.3 -0.9 -1.5 -1.7 -2.0 -2.1 -1.9 0 0.7 2.3 0.3 0.5 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.9 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 -1.9 2.8 2.6 2.0 2.9 5.1 6.0 6.5 -3.5 -6.3 -5.8 -3.3 -1.6 -1.1
Nucleus 0.7 0.9 0 0.8 0.8 0.2 0.9 1.3 0.1 0 0.7 -0.4 3.5 3.1 1.1
Nuclear Periphery -0.9 -3.8 -3.8 -5.1 -6.6 -4.3 -6.9 -6.3 -6.9 -0.1 -0.5 -2.2 -5.1 -4.9 -4.1
Nucleolus -1.0 -0.1 2.0 3.2 3.8 3.7 3.8 4.0 4.5 -3.3 -5.1 -5.5 -3.4 -4.7 -1.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 2.0 0 0 0
Vacuole -0.2 1.0 -0.8 -2.0 -1.8 -0.4 -2.5 -2.0 -2.3 -0.1 2.2 2.0 4.5 5.1 4.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nup57-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available