ORF | |
---|---|
Human Ortholog | |
Description | Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 89 | 127 | 287 | 143 | 186 | 205 | 198 | 188 | 172 | – | 128 | 156 | 101 | 73 | 106 | – | – | – | 29 | 68 | 103 | 38 | 89 | 66 |
Bud | 6 | 14 | 50 | 15 | 38 | 30 | 44 | 43 | 29 | – | 23 | 21 | 3 | 5 | 7 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 4 | 0 | 3 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 82 | 135 | 249 | 132 | 128 | 154 | 144 | 128 | 147 | – | 103 | 131 | 140 | 248 | 225 | – | – | – | 107 | 127 | 314 | 68 | 144 | 83 |
Cytoplasm | 3 | 10 | 34 | 23 | 27 | 50 | 53 | 45 | 28 | – | 31 | 25 | 80 | 157 | 94 | – | – | – | 1 | 1 | 3 | 1 | 31 | 70 |
Endoplasmic Reticulum | 4 | 33 | 12 | 7 | 40 | 30 | 34 | 45 | 23 | – | 23 | 30 | 22 | 32 | 52 | – | – | – | 3 | 12 | 10 | 3 | 42 | 50 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 3 | 0 | 0 | 1 |
Golgi | 4 | 9 | 52 | 7 | 39 | 8 | 22 | 90 | 2 | – | 8 | 36 | 2 | 1 | 1 | – | – | – | 1 | 4 | 3 | 0 | 7 | 5 |
Mitochondria | 77 | 42 | 30 | 37 | 26 | 96 | 120 | 79 | 204 | – | 184 | 154 | 4 | 0 | 1 | – | – | – | 0 | 1 | 14 | 2 | 4 | 1 |
Nucleus | 0 | 0 | 2 | 0 | 2 | 1 | 0 | 3 | 1 | – | 0 | 1 | 0 | 0 | 2 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 1 | 2 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 12 | 5 | 24 | 12 | 9 | 18 | 14 | 9 | 18 | – | 0 | 7 | 5 | 0 | 2 | – | – | – | 0 | 0 | 2 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 6 | 1 | 2 | 0 | 1 | 3 | 1 | – | 0 | 2 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 3 | 1 | 0 | 3 | 0 | 8 | 1 | – | 1 | 3 | 0 | 0 | 0 | – | – | – | 0 | 1 | 3 | 0 | 0 | 0 |
Unique Cell Count | 130 | 208 | 411 | 198 | 298 | 343 | 361 | 386 | 348 | 292 | 319 | 232 | 365 | 342 | 145 | 223 | 470 | 118 | 328 | 290 | ||||
Labelled Cell Count | 277 | 377 | 750 | 378 | 497 | 595 | 630 | 643 | 626 | 502 | 568 | 357 | 516 | 490 | 145 | 223 | 470 | 118 | 328 | 290 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.1 | 13.7 | 10.2 | 9.5 | 11.1 | 9.5 | 10.5 | 11.1 | 9.3 | – | 9.5 | 11.1 | 14.7 | 19.2 | 18.9 | – | – | – | 10.9 | 12.9 | 14.2 |
Std Deviation (1e-4) | 1.9 | 3.1 | 1.6 | 1.4 | 1.6 | 1.7 | 1.6 | 2.0 | 2.1 | – | 2.4 | 2.7 | 2.4 | 4.4 | 5.1 | – | – | – | 2.2 | 2.3 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.12 | -0.1 | 0.04 | 0.12 | -0.13 | – | -0.11 | 0.13 | 0.53 | 0.91 | 0.89 | – | – | – | 0.1 | 0.34 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.6 | -2.1 | -2.9 | -4.3 | -6.1 | -5.7 | – | -6.9 | -5.7 | -6.5 | -13.9 | -10.6 | – | – | – |
Bud | -1.7 | 0.2 | -1.5 | 0 | -0.5 | -1.7 | – | -1.8 | -2.5 | -4.8 | -5.8 | -5.2 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 1.5 | -4.6 | -4.3 | -5.7 | -7.7 | -5.0 | – | -6.6 | -5.2 | -0.1 | 2.1 | 1.5 | – | – | – |
Cytoplasm | 1.3 | 0.4 | 2.7 | 2.8 | 1.6 | -0.1 | – | 1.1 | -0.2 | 8.4 | 11.2 | 7.0 | – | – | – |
Endoplasmic Reticulum | 0.4 | 5.3 | 3.5 | 3.8 | 4.8 | 2.4 | – | 3.0 | 3.7 | 3.6 | 3.5 | 6.0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | -3.6 | 0.2 | -5.2 | -3.1 | 3.9 | -6.4 | – | -4.6 | -0.6 | -5.2 | -6.8 | -6.6 | – | – | – |
Mitochondria | 4.2 | 0.7 | 7.6 | 9.1 | 5.4 | 15.3 | – | 15.8 | 12.6 | -3.0 | -5.3 | -4.8 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0.1 | -1.8 | -0.4 | -1.3 | -2.5 | -0.4 | – | -4.2 | -2.4 | -2.2 | -4.7 | -3.9 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | 1.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.5907 | 17.2879 | 14.5766 | 13.4583 | 9.8424 | 14.0388 | 13.8493 | 17.24 | 15.0858 | 14.9492 | 12.621 | 16.0362 | 13.3364 | 15.3022 | 13.6455 | 12.9274 | 12.6583 | 13.0282 |
Actin | 0.1217 | 0.0151 | 0.0368 | 0.0031 | 0.2426 | 0.0415 | 0.0651 | 0.0353 | 0.0576 | 0.0369 | 0.1068 | 0.0322 | 0.1546 | 0.0504 | 0.1058 | 0.0665 | 0.1331 | 0.0692 |
Bud | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0005 | 0.0004 | 0.0007 | 0.0001 | 0.0006 | 0.0004 | 0.0021 | 0.0002 | 0.0008 | 0.0003 | 0.0011 | 0.0008 | 0.0004 | 0.0004 |
Bud Neck | 0.0051 | 0.0032 | 0.0061 | 0.0029 | 0.0118 | 0.0033 | 0.0027 | 0.003 | 0.0034 | 0.0047 | 0.0118 | 0.0012 | 0.0041 | 0.0086 | 0.0127 | 0.0206 | 0.0077 | 0.0042 |
Bud Periphery | 0.0017 | 0.0008 | 0.0016 | 0.0016 | 0.0027 | 0.0048 | 0.0041 | 0.0007 | 0.0027 | 0.0034 | 0.0049 | 0.0017 | 0.0025 | 0.0014 | 0.0039 | 0.0057 | 0.0027 | 0.0027 |
Bud Site | 0.0075 | 0.1013 | 0.0192 | 0.0031 | 0.002 | 0.0012 | 0.0114 | 0.0175 | 0.0081 | 0.0041 | 0.0256 | 0.0007 | 0.0103 | 0.0474 | 0.0358 | 0.0172 | 0.0034 | 0.004 |
Cell Periphery | 0.0537 | 0.2105 | 0.2387 | 0.2755 | 0.1816 | 0.2138 | 0.1349 | 0.1356 | 0.1816 | 0.2499 | 0.1197 | 0.1832 | 0.0632 | 0.1294 | 0.1612 | 0.3247 | 0.1067 | 0.1465 |
Cytoplasm | 0.0037 | 0.0722 | 0.0048 | 0.0031 | 0.0013 | 0.0019 | 0.0107 | 0.0026 | 0.0099 | 0.0064 | 0.0042 | 0.0017 | 0.0052 | 0.0064 | 0.0096 | 0.0112 | 0.0057 | 0.0101 |
Cytoplasmic Foci | 0.0066 | 0.0016 | 0.0004 | 0.0001 | 0.0041 | 0.014 | 0.0057 | 0.0001 | 0.0067 | 0.0056 | 0.0142 | 0.0005 | 0.0218 | 0.0002 | 0.0033 | 0.0142 | 0.0042 | 0.0015 |
Eisosomes | 0.7344 | 0.5826 | 0.6731 | 0.7031 | 0.3463 | 0.6681 | 0.7024 | 0.7987 | 0.6844 | 0.6271 | 0.6139 | 0.7516 | 0.6916 | 0.7523 | 0.6354 | 0.4787 | 0.6817 | 0.7392 |
Endoplasmic Reticulum | 0.0015 | 0.0029 | 0.0008 | 0.0032 | 0.0013 | 0.0021 | 0.0061 | 0.0034 | 0.005 | 0.0181 | 0.0042 | 0.0038 | 0.0047 | 0.0013 | 0.0035 | 0.0089 | 0.0036 | 0.0027 |
Endosome | 0.006 | 0.0012 | 0.0001 | 0 | 0.0059 | 0.0037 | 0.007 | 0 | 0.0126 | 0.0028 | 0.0223 | 0.0014 | 0.0081 | 0 | 0.0029 | 0.0134 | 0.0073 | 0.0007 |
Golgi | 0.0051 | 0.0006 | 0.0007 | 0.0003 | 0.0069 | 0.0048 | 0.0059 | 0.001 | 0.0046 | 0.0097 | 0.0238 | 0.0025 | 0.0068 | 0.0006 | 0.0042 | 0.0082 | 0.0047 | 0.0016 |
Lipid Particles | 0.0142 | 0.0003 | 0.0005 | 0.0001 | 0.0406 | 0.0084 | 0.0122 | 0.0001 | 0.004 | 0.0053 | 0.0123 | 0.0007 | 0.0058 | 0.0002 | 0.0043 | 0.0069 | 0.0082 | 0.0005 |
Mitochondria | 0.0154 | 0.0012 | 0.0137 | 0.0018 | 0.1386 | 0.005 | 0.0107 | 0.0002 | 0.0029 | 0.0158 | 0.014 | 0.0159 | 0.0029 | 0.0002 | 0.0096 | 0.0021 | 0.0116 | 0.0127 |
None | 0.0137 | 0.0038 | 0.0006 | 0.0009 | 0.001 | 0.008 | 0.0039 | 0.0004 | 0.0009 | 0.0005 | 0.0016 | 0.001 | 0.0034 | 0.0004 | 0.0012 | 0.006 | 0.0051 | 0.0007 |
Nuclear Periphery | 0.0017 | 0.0005 | 0.0002 | 0.0003 | 0.0008 | 0.0004 | 0.0017 | 0.0007 | 0.0033 | 0.0015 | 0.0055 | 0.0005 | 0.0016 | 0.0004 | 0.0009 | 0.0037 | 0.004 | 0.0008 |
Nucleolus | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0014 | 0 | 0.0004 | 0.0001 | 0.0005 | 0 | 0.0002 | 0 | 0.0001 | 0.0006 | 0.0003 | 0 |
Nucleus | 0.0003 | 0.0004 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0009 | 0 | 0.0054 | 0.0001 | 0.0008 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0011 | 0.0004 | 0.0004 |
Peroxisomes | 0.0036 | 0.0002 | 0.0015 | 0.0002 | 0.0078 | 0.016 | 0.0076 | 0.0001 | 0.0024 | 0.0068 | 0.0071 | 0.0003 | 0.0058 | 0.0001 | 0.0026 | 0.0043 | 0.002 | 0.0005 |
Punctate Nuclear | 0.0026 | 0.0001 | 0 | 0 | 0.0007 | 0.0008 | 0.0035 | 0 | 0.0022 | 0.0001 | 0.0023 | 0.0001 | 0.005 | 0 | 0.0004 | 0.0034 | 0.0067 | 0.0012 |
Vacuole | 0.0005 | 0.001 | 0.0007 | 0.0004 | 0.0015 | 0.0013 | 0.0011 | 0.0003 | 0.0008 | 0.0007 | 0.0015 | 0.0005 | 0.001 | 0.0003 | 0.0009 | 0.0017 | 0.0003 | 0.0003 |
Vacuole Periphery | 0.0003 | 0.0001 | 0 | 0 | 0.0016 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0004 | 0.0003 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.6089 | 20.9268 | 24.4874 | 44.2066 | 28.6006 | 39.1444 | 36.1128 | 45.5891 | 54.6188 | 35.4943 |
Translational Efficiency | 2.2054 | 2.1371 | 2.6099 | 2.2673 | 2.1852 | 1.7884 | 1.5023 | 2.0978 | 1.8926 | 1.8986 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2054 | 1639 | 2481 | 1773 | 1691 | 1470 | 557 | 2794 | 3745 | 3109 | 3038 | 4567 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 976.47 | 1173.09 | 2187.62 | 1868.32 | 1078.18 | 1027.66 | 1626.82 | 1920.69 | 1022.40 | 1104.33 | 2084.80 | 1900.36 |
Standard Deviation | 139.57 | 186.96 | 447.66 | 430.77 | 161.59 | 182.98 | 319.70 | 443.23 | 158.23 | 198.82 | 479.05 | 439.18 |
Intensity Change Log 2 | — | 0.264666 | 1.163715 | 0.936094 | — | -0.069235 | 0.593457 | 0.833027 | — | 0.099103 | 0.892579 | 0.882928 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.139898* | 0.091459 | 0.033236 | 0.063835 | 0.086100 | 0.124523* | 0.127769* | 0.066565 | 0.115606* | 0.107092* | 0.050568 | 0.065505 |
Bud Neck | 0.003862 | 0.005440 | 0.005287 | 0.004109 | 0.004281 | 0.004634 | 0.006633 | 0.003294 | 0.004051 | 0.005059 | 0.005534 | 0.003611 |
Bud Site | 0.001403 | 0.003614 | 0.002597 | 0.007929 | 0.002295 | 0.004464 | 0.002723 | 0.004798 | 0.001806 | 0.004016 | 0.002620 | 0.006013 |
Cell Periphery | 0.194810* | 0.378051* | 0.445996* | 0.532816* | 0.233235* | 0.305894* | 0.079178 | 0.538167* | 0.212161* | 0.343934* | 0.378742* | 0.536089* |
Cytoplasm | 0.000254 | 0.000351 | 0.000830 | 0.001269 | 0.000194 | 0.000968 | 0.000338 | 0.003128 | 0.000227 | 0.000643 | 0.000739 | 0.002406 |
Cytoplasmic Foci | 0.005524 | 0.001285 | 0.000491 | 0.000625 | 0.002359 | 0.002519 | 0.001959 | 0.000975 | 0.004095 | 0.001868 | 0.000760 | 0.000839 |
Eisosomes | 0.618291* | 0.494445* | 0.384209* | 0.369330* | 0.653320* | 0.491734* | 0.444638* | 0.334592* | 0.634108* | 0.493163* | 0.395289* | 0.348078* |
Endoplasmic Reticulum | 0.003365 | 0.007397 | 0.033531 | 0.006300 | 0.005702 | 0.011513 | 0.120801* | 0.008382 | 0.004420 | 0.009343 | 0.049532 | 0.007574 |
Endosome | 0.002195 | 0.003236 | 0.005603 | 0.002211 | 0.002374 | 0.009536 | 0.027894 | 0.004106 | 0.002276 | 0.006215 | 0.009690 | 0.003371 |
Golgi | 0.003915 | 0.001911 | 0.005658 | 0.001957 | 0.001628 | 0.011833 | 0.084894 | 0.003841 | 0.002882 | 0.006603 | 0.020185 | 0.003109 |
Lipid Particles | 0.005623 | 0.001442 | 0.055542 | 0.004019 | 0.000702 | 0.009950 | 0.093687 | 0.010503 | 0.003401 | 0.005465 | 0.062536 | 0.007986 |
Mitochondria | 0.001117 | 0.001751 | 0.000788 | 0.001735 | 0.001046 | 0.002008 | 0.000375 | 0.002637 | 0.001085 | 0.001873 | 0.000712 | 0.002287 |
Mitotic Spindle | 0.001800 | 0.001634 | 0.001556 | 0.000577 | 0.002424 | 0.001393 | 0.000358 | 0.000827 | 0.002082 | 0.001520 | 0.001336 | 0.000730 |
None | 0.001770 | 0.000449 | 0.000262 | 0.000166 | 0.001087 | 0.000582 | 0.000250 | 0.000227 | 0.001462 | 0.000512 | 0.000260 | 0.000203 |
Nuclear Periphery | 0.000258 | 0.000426 | 0.002212 | 0.000354 | 0.000361 | 0.000361 | 0.000350 | 0.000637 | 0.000304 | 0.000395 | 0.001870 | 0.000527 |
Nuclear Periphery Foci | 0.000015 | 0.000096 | 0.000083 | 0.000032 | 0.000028 | 0.000156 | 0.000097 | 0.000032 | 0.000021 | 0.000125 | 0.000085 | 0.000032 |
Nucleolus | 0.000005 | 0.000042 | 0.000014 | 0.000012 | 0.000007 | 0.000031 | 0.000003 | 0.000010 | 0.000006 | 0.000037 | 0.000012 | 0.000011 |
Nucleus | 0.000169 | 0.000320 | 0.000246 | 0.000153 | 0.000180 | 0.000382 | 0.000097 | 0.000173 | 0.000174 | 0.000349 | 0.000219 | 0.000165 |
Peroxisomes | 0.015628 | 0.006391 | 0.019735 | 0.000988 | 0.002490 | 0.017061 | 0.007076 | 0.016062 | 0.009696 | 0.011436 | 0.017414 | 0.010210 |
Vacuole | 0.000068 | 0.000170 | 0.001985 | 0.001165 | 0.000126 | 0.000213 | 0.000792 | 0.000820 | 0.000094 | 0.000191 | 0.001766 | 0.000954 |
Vacuole Periphery | 0.000031 | 0.000090 | 0.000139 | 0.000419 | 0.000059 | 0.000243 | 0.000090 | 0.000225 | 0.000044 | 0.000162 | 0.000130 | 0.000300 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 6.57 | 18.40* | 11.38 | 4.36 | -6.95 | -4.92 | -4.09 | 3.37 | 8.31 | 6.40 | 1.57 | 14.67 | 11.21 | 8.91 | -4.29 |
Bud Neck | -4.42 | -4.30 | -0.77 | 3.31 | 3.10 | -1.04 | -3.81 | 3.25 | 3.79 | 5.34 | -4.07 | -5.24 | 1.99 | 5.41 | 6.39 |
Bud Site | -4.63 | -5.46 | -5.10 | -3.22 | -4.19 | -2.76 | -0.93 | -4.23 | -0.43 | -3.47 | -4.95 | -3.71 | -6.96 | -2.85 | -5.69 |
Cell Periphery | -18.89* | -28.21* | -34.17* | -13.66 | -8.14 | -6.83 | 16.28 | -32.76* | -22.29 | -49.77* | -18.31 | -21.74 | -48.68 | -25.20 | -19.50 |
Cytoplasm | -1.48 | -3.41 | -2.68 | -2.40 | -1.15 | -2.72 | -1.94 | -4.97 | -3.32 | -4.73 | -3.01 | -3.65 | -5.60 | -4.30 | -4.07 |
Cytoplasmic Foci | 5.68 | 7.44 | 6.93 | 1.75 | -0.65 | -0.30 | 0.77 | 3.74 | 3.46 | 2.31 | 4.67 | 8.16 | 7.75 | 3.58 | -0.55 |
Eisosomes | 11.72 | 25.01 | 24.46 | 11.48 | 1.54 | 13.72 | 11.63 | 33.37* | 14.90 | 6.41 | 17.90 | 30.34 | 41.71 | 19.44 | 6.33 |
Endoplasmic Reticulum | -3.93 | -15.74 | -3.09 | 0.91 | 13.44 | -4.14 | -14.36 | -2.75 | 2.22 | 14.02 | -5.76 | -20.22 | -4.71 | 1.93 | 18.59 |
Endosome | -2.90 | -13.39 | -0.07 | 2.46 | 10.29 | -7.10 | -11.80 | -5.45 | 5.38 | 11.00 | -7.65 | -15.60 | -5.17 | 5.39 | 12.94 |
Golgi | 3.33 | -2.64 | 2.61 | -0.09 | 5.17 | -6.76 | -12.55 | -2.87 | 5.21 | 12.21 | -4.73 | -12.13 | -0.43 | 4.28 | 11.83 |
Lipid Particles | 6.12 | -15.71 | 1.31 | -2.44 | 15.72 | -6.64 | -12.89 | -9.57 | -0.34 | 11.42 | -2.71 | -20.41 | -5.57 | -2.53 | 18.36 |
Mitochondria | -0.93 | 0.92 | -1.47 | 0.02 | -3.97 | -1.72 | 6.21 | -3.71 | -0.91 | -5.33 | -1.75 | 1.86 | -3.57 | -0.86 | -5.74 |
Mitotic Spindle | 1.05 | 1.72 | 8.32 | 9.12 | 10.50 | 5.07 | 11.99 | 9.25 | 4.75 | -9.56 | 4.44 | 6.46 | 12.31 | 9.83 | 9.85 |
None | 12.85 | 14.97 | 16.02 | 10.76 | 6.05 | 5.88 | 6.45 | 12.88 | 6.41 | 0.21 | 13.76 | 17.99 | 20.00 | 10.39 | 2.33 |
Nuclear Periphery | -5.78 | -25.55 | -4.76 | 2.43 | 24.22 | -0.03 | 0.31 | -10.41 | -8.62 | -7.17 | -4.64 | -24.53 | -12.30 | -5.71 | 20.69 |
Nuclear Periphery Foci | -1.20 | -15.19 | -2.87 | 0.95 | 6.97 | -1.07 | -5.07 | -0.70 | 1.03 | 4.97 | -1.55 | -13.30 | -2.88 | 1.38 | 10.23 |
Nucleolus | -1.02 | -15.43 | -7.43 | 0.82 | 1.80 | -1.05 | 6.00 | -4.14 | 0.91 | -15.55 | -1.40 | -11.48 | -9.13 | 1.17 | 1.56 |
Nucleus | -0.85 | -7.56 | -0.03 | 0.82 | 1.69 | -0.89 | 8.62 | 0.67 | 0.92 | -9.22 | -1.23 | -5.47 | 0.12 | 1.24 | 2.51 |
Peroxisomes | 5.82 | -2.03 | 11.88 | 5.24 | 11.58 | -8.65 | -3.87 | -10.36 | 0.48 | -5.31 | -1.48 | -5.13 | -0.49 | 1.00 | 4.66 |
Vacuole | -5.24 | -6.10 | -2.57 | -2.34 | 1.44 | -1.69 | -7.01 | -4.09 | -3.51 | -0.24 | -3.70 | -6.49 | -4.40 | -3.89 | 2.40 |
Vacuole Periphery | -1.57 | -6.57 | -1.68 | -1.41 | -1.22 | -1.75 | -1.31 | -2.70 | 0.14 | -2.34 | -2.23 | -6.27 | -2.62 | -1.28 | -1.77 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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