ORF
Description Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.68 0.61 0.7 0.72 0.62 0.6 0.55 0.49 0.49 – 0.44 0.49 0.44 0.2 0.31 – – – 0.2 0.31 0.22 0.33 0.27 0.23
Bud 0.05 0.07 0.12 0.08 0.13 0.09 0.12 0.11 0.08 – 0.08 0.07 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0.63 0.65 0.61 0.67 0.43 0.45 0.4 0.33 0.42 – 0.35 0.41 0.6 0.68 0.66 – – – 0.74 0.57 0.67 0.58 0.44 0.29
Cytoplasm 0 0.05 0.08 0.12 0.09 0.15 0.15 0.12 0.08 – 0.11 0.08 0.34 0.43 0.27 – – – 0 0 0 0 0.1 0.24
Endoplasmic Reticulum 0 0.16 0 0 0.13 0.09 0.09 0.12 0.07 – 0.08 0.09 0.09 0.09 0.15 – – – 0 0.06 0 0 0.13 0.17
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0.13 0 0.13 0 0.06 0.23 0 – 0 0.11 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.59 0.2 0.07 0.19 0.09 0.28 0.33 0.2 0.59 – 0.63 0.48 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0.09 0 0.06 0.06 0 0.05 0 0 0.05 – 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 89 127 287 143 186 205 198 188 172 – 128 156 101 73 106 – – – 29 68 103 38 89 66
Bud 6 14 50 15 38 30 44 43 29 – 23 21 3 5 7 – – – 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 4 0 3 2
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – – – – –
Cell Periphery 82 135 249 132 128 154 144 128 147 – 103 131 140 248 225 – – – 107 127 314 68 144 83
Cytoplasm 3 10 34 23 27 50 53 45 28 – 31 25 80 157 94 – – – 1 1 3 1 31 70
Endoplasmic Reticulum 4 33 12 7 40 30 34 45 23 – 23 30 22 32 52 – – – 3 12 10 3 42 50
Endosome 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 1 3 0 0 1
Golgi 4 9 52 7 39 8 22 90 2 – 8 36 2 1 1 – – – 1 4 3 0 7 5
Mitochondria 77 42 30 37 26 96 120 79 204 – 184 154 4 0 1 – – – 0 1 14 2 4 1
Nucleus 0 0 2 0 2 1 0 3 1 – 0 1 0 0 2 – – – 0 0 1 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 2 0 – 1 2 0 0 0 – – – 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 12 5 24 12 9 18 14 9 18 – 0 7 5 0 2 – – – 0 0 2 0 0 0
SpindlePole 0 0 6 1 2 0 1 3 1 – 0 2 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 1 3 1 0 3 0 8 1 – 1 3 0 0 0 – – – 0 1 3 0 0 0
Unique Cell Count 130 208 411 198 298 343 361 386 348 292 319 232 365 342 145 223 470 118 328 290
Labelled Cell Count 277 377 750 378 497 595 630 643 626 502 568 357 516 490 145 223 470 118 328 290


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.1 13.7 10.2 9.5 11.1 9.5 10.5 11.1 9.3 – 9.5 11.1 14.7 19.2 18.9 – – – 10.9 12.9 14.2
Std Deviation (1e-4) 1.9 3.1 1.6 1.4 1.6 1.7 1.6 2.0 2.1 – 2.4 2.7 2.4 4.4 5.1 – – – 2.2 2.3 2.6
Intensity Change (Log2) – – – -0.1 0.12 -0.1 0.04 0.12 -0.13 – -0.11 0.13 0.53 0.91 0.89 – – – 0.1 0.34 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.6 -2.1 -2.9 -4.3 -6.1 -5.7 – -6.9 -5.7 -6.5 -13.9 -10.6 – – –
Bud -1.7 0.2 -1.5 0 -0.5 -1.7 – -1.8 -2.5 -4.8 -5.8 -5.2 – – –
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Cell Periphery 1.5 -4.6 -4.3 -5.7 -7.7 -5.0 – -6.6 -5.2 -0.1 2.1 1.5 – – –
Cytoplasm 1.3 0.4 2.7 2.8 1.6 -0.1 – 1.1 -0.2 8.4 11.2 7.0 – – –
Endoplasmic Reticulum 0.4 5.3 3.5 3.8 4.8 2.4 – 3.0 3.7 3.6 3.5 6.0 – – –
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Golgi -3.6 0.2 -5.2 -3.1 3.9 -6.4 – -4.6 -0.6 -5.2 -6.8 -6.6 – – –
Mitochondria 4.2 0.7 7.6 9.1 5.4 15.3 – 15.8 12.6 -3.0 -5.3 -4.8 – – –
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Peroxisomes 0.1 -1.8 -0.4 -1.3 -2.5 -0.4 – -4.2 -2.4 -2.2 -4.7 -3.9 – – –
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Vacuole 0 0 0 0 1.6 0 – 0 0 0 0 0 – – –