Standard name
Human Ortholog
Description Peroxisomal ubiquitin conjugating enzyme; required for peroxisomal matrix protein import and peroxisome biogenesis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0.16 0.06 0.05 0 0 0 0.07 0.12 0.09 0.09 0.13 0.16
Bud 0.05 0.07 0.17 0.11 0.14 0.14 0.11 0.12 0.13 0.1 0.13 0.13 0.05 0 0.06 0.1 0.09 0.08 0 0 0 0 0 0
Bud Neck 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0.1 0.05 0 0 0 0 0 0 0 0.05 0 0
Bud Site 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0.05
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.45 0.42 0.21 0.26 0.24 0.26 0.24 0.28 0.3 0.37 0.31 0.31 0.4 0.42 0.42 0.09 0.07 0.09 0.11 0.1 0 0.06 0.09 0.11
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.06 0.07 0 0.05 0.09 0 0 0 0 0 0 0 0 0.05 0.12 0.2 0.2 0.2 0.16 0.16 0.18 0.15 0.14 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.2 0.27 0.28 0.34 0.57 0.56 0.48 0.62 0.53 0.69 0.68 0 0 0 0.16 0.08 0.11 0.19 0.21 0.13 0.22 0.19 0.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.06 0.2 0.22 0.34 0.33 0.15 0.13 0.1 0.08 0.07 0.06 0.07 0.08 0.15 0.14 0.27 0.37 0.39 0.28 0.18 0.44 0.29 0.27 0.26
SpindlePole 0.32 0.1 0.28 0.26 0.3 0.22 0.24 0.27 0.19 0.23 0.11 0.12 0.36 0.4 0.36 0.46 0.42 0.48 0.11 0.09 0.08 0.06 0.08 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 8 2 2 0 7 1 0 0 0 0 2 50 27 23 6 4 13 20 30 19 26 60 83
Bud 11 12 27 31 61 72 73 93 61 55 52 73 17 14 29 43 39 33 4 7 3 7 7 12
Bud Neck 14 2 9 4 8 9 13 16 4 5 4 2 30 20 22 10 12 7 7 9 4 15 17 15
Bud Site 8 2 6 10 11 31 38 29 14 6 13 14 9 7 4 10 16 22
Cell Periphery 1 0 0 0 0 0 3 0 0 1 1 3 3 0 1 0 0 0 0 1 0 2 1 7
Cytoplasm 99 70 34 72 102 131 163 219 134 197 129 179 125 175 212 40 31 38 31 25 5 18 42 53
Endoplasmic Reticulum 1 4 2 3 0 1 0 3 0 0 0 1 4 4 10 3 10 5 0 1 0 2 3 2
Endosome 13 12 3 13 41 7 10 20 6 9 2 4 8 21 61 84 82 85 45 42 38 43 66 50
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 6 6 4 2 4 0 3 2 4
Mitochondria 35 33 43 78 147 293 373 375 281 286 286 388 6 1 3 70 32 48 53 55 29 64 93 99
Nucleus 0 0 0 1 2 4 4 4 2 1 1 1 0 0 0 7 5 9 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 1 1 0 1 0 0 0 0 0 4 4 2 0 0 0 0 0 0
Nucleolus 0 0 2 2 0 2 5 3 2 2 0 0 1 0 2 15 10 11 0 1 0 1 0 0
Peroxisomes 14 34 35 94 144 78 85 80 34 38 23 42 24 63 71 115 154 169 80 46 94 86 128 130
SpindlePole 71 17 45 71 128 114 162 206 87 122 45 70 113 168 182 195 175 209 31 23 17 18 38 23
Vac/Vac Membrane 1 2 1 1 1 2 2 5 5 4 5 1 1 1 4 3 5 3 4 3 1 4 10 14
Unique Cell Count 221 166 159 277 433 510 672 774 452 536 412 570 313 417 510 426 416 432 287 258 217 297 482 505
Labelled Cell Count 271 197 210 382 645 751 933 1054 630 727 561 780 391 501 624 611 585 658 287 258 217 297 482 505


Peroxisomes

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.5 3.9 3.6 3.5 3.4 2.9 3.2 2.9 2.8 2.8 2.9 5.1 5.0 5.1 5.5 5.6 5.6 5.6 5.4 5.4
Std Deviation (1e-4) 1.1 0.9 1.0 1.1 1.5 1.3 1.0 1.2 1.1 1.0 0.8 0.7 0.7 0.8 0.9 1.1 1.2 1.3 1.5 1.3 1.4
Intensity Change (Log2) -0.1 -0.12 -0.17 -0.43 -0.28 -0.4 -0.44 -0.46 -0.42 0.39 0.38 0.39 0.51 0.53 0.52 0.53 0.48 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9372 0.0806 1.0461 0.8222 0.4207 0.43 0.1753 0.8683 0.8285 0.5741 1.1143 0.9494 -1.2809 -0.8634 -1.2629 -1.1838 -1.4111 -1.0747
Actin 0.0725 0.0468 0.0482 0.0132 0.0156 0.0083 0.0395 0.0225 0.0275 0.0092 0.0264 0.0113 0.0506 0.0154 0.0218 0.0028 0.0101 0.0174
Bud 0.001 0.0035 0.0032 0.0014 0.0021 0.0008 0.0008 0.0025 0.0033 0.0016 0.0002 0.0003 0.0003 0.0013 0.0008 0.0002 0.0001 0.0002
Bud Neck 0.0071 0.0016 0.0167 0.0379 0.0387 0.0147 0.0149 0.0146 0.029 0.0056 0.0025 0.0225 0.0058 0.0093 0.0356 0.0032 0.0027 0.0111
Bud Periphery 0.0017 0.0099 0.0049 0.0039 0.0044 0.0024 0.002 0.0052 0.01 0.006 0.0004 0.0009 0.0006 0.0019 0.0018 0.0003 0.0003 0.0005
Bud Site 0.0132 0.0223 0.0366 0.0012 0.0096 0.0024 0.0048 0.0423 0.0147 0.0169 0.0011 0.0018 0.0088 0.0459 0.0223 0.0006 0.0014 0.0004
Cell Periphery 0.0011 0.0006 0.0006 0.0002 0.0005 0.0005 0.0008 0.0011 0.0009 0.0027 0.0001 0.0005 0.0007 0.0008 0.0005 0 0.0003 0.0002
Cytoplasm 0.0121 0.0258 0.0231 0.0131 0.0088 0.0228 0.0219 0.0304 0.019 0.0109 0.0039 0.0287 0.0254 0.0558 0.0275 0.0106 0.0285 0.0337
Cytoplasmic Foci 0.2321 0.1881 0.1516 0.1675 0.2139 0.303 0.2843 0.2292 0.2205 0.217 0.2782 0.3193 0.3331 0.318 0.3149 0.4201 0.3282 0.3943
Eisosomes 0.0012 0.0003 0.0005 0.0004 0.0002 0.0003 0.0021 0.0012 0.0016 0.0015 0.001 0.0006 0.0009 0.0007 0.0005 0.0001 0.0003 0.0003
Endoplasmic Reticulum 0.0027 0.0007 0.0013 0.0006 0.0005 0.0009 0.0021 0.0005 0.0009 0.0003 0.0006 0.0005 0.0027 0.0017 0.0007 0.0003 0.0008 0.001
Endosome 0.0772 0.0162 0.0352 0.0256 0.0264 0.0301 0.019 0.0307 0.032 0.0549 0.0183 0.0221 0.0358 0.0419 0.0308 0.0392 0.0574 0.0269
Golgi 0.0243 0.007 0.0163 0.0254 0.0442 0.0074 0.0112 0.0063 0.0107 0.0193 0.0067 0.0073 0.0098 0.0107 0.0114 0.0157 0.0138 0.0097
Lipid Particles 0.0494 0.0307 0.0159 0.0142 0.0172 0.046 0.0244 0.0234 0.0221 0.0562 0.0478 0.0445 0.0688 0.0256 0.0164 0.0449 0.102 0.0379
Mitochondria 0.0106 0.0048 0.0334 0.0052 0.0069 0.0155 0.0045 0.0128 0.016 0.026 0.0095 0.0113 0.005 0.0056 0.0136 0.0056 0.0028 0.0065
None 0.124 0.2373 0.1763 0.2629 0.044 0.2008 0.2626 0.3401 0.3361 0.1204 0.1997 0.2182 0.1968 0.2727 0.2227 0.0908 0.0894 0.1228
Nuclear Periphery 0.0036 0.0005 0.0051 0.0008 0.0034 0.0038 0.0065 0.0002 0.0006 0.0001 0.0045 0.0008 0.0036 0.0008 0.0004 0.0003 0.0002 0.0028
Nucleolus 0.004 0.0015 0.0017 0.0042 0.0016 0.0029 0.0017 0.0008 0.0015 0.0011 0.0045 0.0009 0.0039 0.0012 0.0009 0.0002 0.0003 0.0002
Nucleus 0.0035 0.0015 0.0031 0.0023 0.0025 0.002 0.0077 0.0005 0.0008 0.0006 0.0184 0.0006 0.0037 0.0011 0.0007 0.0002 0.0002 0.0006
Peroxisomes 0.313 0.3729 0.4072 0.3807 0.5371 0.316 0.2825 0.2315 0.2451 0.4249 0.3389 0.2773 0.235 0.1796 0.2625 0.3613 0.3526 0.3285
Punctate Nuclear 0.0324 0.0265 0.0131 0.0376 0.0209 0.0155 0.0048 0.0029 0.0057 0.0205 0.0359 0.0287 0.0043 0.0063 0.0126 0.0028 0.0063 0.004
Vacuole 0.0107 0.0015 0.0048 0.0013 0.001 0.0031 0.0017 0.0011 0.0016 0.0037 0.0009 0.0017 0.0038 0.003 0.0013 0.0006 0.0021 0.0006
Vacuole Periphery 0.0027 0.0003 0.0012 0.0004 0.0003 0.0008 0.0005 0.0002 0.0004 0.0008 0.0005 0.0003 0.0006 0.0005 0.0003 0.0002 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.9919 3.5738 6.6298 11.8526 19.4377 5.9934 15.3573 18.2915 10.8211 12.0088
Translational Efficiency 1.1054 4.2479 1.962 0.8311 0.5492 2.7357 1.3685 0.8213 1.6144 1.3215

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1392 1733 2913 1049 1403 1432 2513 1321 2795 3165 5426 2370

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 560.43 704.33 813.21 816.97 676.90 668.31 786.54 841.88 618.89 688.03 800.86 830.85
Standard Deviation 65.49 92.54 97.23 110.62 80.24 94.35 100.58 113.16 93.59 95.07 99.69 112.72
Intensity Change Log 2 0.329717 0.537094 0.543749 -0.018425 0.216577 0.314672 0.149723 0.370616 0.422953

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002315 0.005144 0.016230 0.056150 0.004865 0.006426 0.033639 0.048394 0.003595 0.005724 0.024293 0.051827
Bud Neck 0.041129 0.043216 0.044623 0.011475 0.034174 0.040589 0.029743 0.018663 0.037638 0.042028 0.037731 0.015482
Bud Site 0.008914 0.018293 0.044173 0.079787 0.008329 0.023361 0.051128 0.053968 0.008620 0.020586 0.047394 0.065396
Cell Periphery 0.000723 0.000163 0.000292 0.000474 0.000260 0.000400 0.000368 0.000689 0.000491 0.000270 0.000327 0.000594
Cytoplasm 0.211018* 0.163981* 0.192232* 0.239236* 0.189805* 0.124657* 0.164849* 0.265239* 0.200370* 0.146189* 0.179550* 0.253730*
Cytoplasmic Foci 0.361020* 0.343980* 0.152110 0.111419 0.374758* 0.309891* 0.134849 0.155545 0.367916* 0.328557* 0.144115 0.136014
Eisosomes 0.001448 0.000388 0.000193 0.000432 0.000876 0.000715 0.000231 0.000827 0.001161 0.000536 0.000211 0.000652
Endoplasmic Reticulum 0.001714 0.000436 0.001633 0.003282 0.000953 0.000981 0.000797 0.001890 0.001332 0.000683 0.001246 0.002506
Endosome 0.013254 0.033349 0.022513 0.013589 0.021444 0.045017 0.022793 0.014699 0.017365 0.038628 0.022643 0.014208
Golgi 0.022261 0.025616 0.003175 0.003782 0.015717 0.040190 0.004408 0.004531 0.018976 0.032210 0.003746 0.004200
Lipid Particles 0.048184 0.017128 0.017828 0.007211 0.031485 0.025298 0.007327 0.007366 0.039801 0.020824 0.012965 0.007297
Mitochondria 0.059089 0.024588 0.006898 0.003119 0.015887 0.057867 0.004605 0.002679 0.037403 0.039645 0.005836 0.002874
Mitotic Spindle 0.000713 0.008482 0.075316 0.034051 0.004987 0.012306 0.049009 0.010521 0.002858 0.010212 0.063132 0.020935
None 0.006339 0.001010 0.002114 0.001150 0.001134 0.002100 0.000926 0.002170 0.003726 0.001503 0.001564 0.001719
Nuclear Periphery 0.000304 0.000230 0.001320 0.000493 0.000295 0.000266 0.000358 0.000578 0.000299 0.000247 0.000874 0.000541
Nuclear Periphery Foci 0.000267 0.000277 0.001630 0.001444 0.000394 0.000322 0.000664 0.001221 0.000331 0.000297 0.001183 0.001320
Nucleolus 0.001511 0.001040 0.003502 0.000402 0.000955 0.001101 0.000829 0.000502 0.001232 0.001067 0.002264 0.000458
Nucleus 0.070785 0.048105 0.147868* 0.074581 0.065770 0.028882 0.042661 0.109750 0.068267 0.039407 0.099142 0.094184
Peroxisomes 0.139431* 0.231545* 0.234743* 0.209644* 0.202134* 0.247509* 0.403356* 0.182229* 0.170906* 0.238768* 0.312834* 0.194363*
Vacuole 0.008263 0.032103 0.031127 0.146830 0.024865 0.028839 0.046876 0.116884 0.016596 0.030626 0.038421 0.130139
Vacuole Periphery 0.001319 0.000927 0.000480 0.001446 0.000914 0.003283 0.000586 0.001654 0.001116 0.001993 0.000529 0.001562

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.80 -10.06 -10.15 -9.25 -6.28 -1.52 -12.15 -9.75 -9.22 -2.07 -3.36 -15.49 -14.04 -13.08 -6.05
Bud Neck -0.52 -2.04 9.74 12.86 18.86 -1.78 0.83 5.96 7.65 6.89 -1.67 -1.30 10.95 13.93 17.71
Bud Site -6.03 -15.61 -11.34 -9.00 -3.17 -7.30 -16.43 -11.41 -5.94 0.59 -9.54 -22.71 -16.04 -10.83 -1.91
Cell Periphery 6.76 5.37 3.64 -6.13 -3.31 -2.30 -1.54 -6.07 -2.63 -3.44 4.42 3.61 -0.38 -5.78 -4.88
Cytoplasm 5.47 2.12 -3.82 -8.73 -6.04 7.92 0.58 -7.75 -15.58 -8.96 9.05 1.82 -8.25 -16.78 -10.69
Cytoplasmic Foci 1.93 30.12 36.89 37.14 11.14 7.39 33.38 29.84 22.32 -0.16 6.42 44.75 45.83 40.57 6.57
Eisosomes 7.29 8.79 7.39 0.30 -6.56 1.15 6.21 1.59 0.30 -7.08 6.35 10.76 6.43 -0.19 -8.94
Endoplasmic Reticulum 2.61 -1.33 -3.63 -9.35 -3.79 -0.04 -1.01 -6.59 -7.01 -7.09 2.41 -1.78 -5.85 -12.31 -6.41
Endosome -8.95 -4.33 2.03 10.66 6.45 -8.58 -0.26 4.85 12.50 5.28 -12.07 -2.99 4.84 16.13 8.08
Golgi -1.29 12.88 12.73 13.87 0.96 -8.92 9.95 9.16 14.32 0.66 -7.55 16.29 15.42 19.55 0.87
Lipid Particles 11.43 11.90 16.91 6.85 9.78 2.33 11.67 12.06 10.10 1.34 10.10 16.16 20.63 11.99 9.35
Mitochondria 8.51 13.86 15.06 11.99 6.47 -10.80 7.33 8.88 15.44 4.32 -0.73 15.25 17.00 19.31 8.29
Mitotic Spindle -7.08 -22.23* -7.83 -5.21 10.73 -3.61 -14.47 -2.76 1.02 12.52 -6.58 -26.15 -8.21 -3.74 16.90
None 4.23 3.11 3.96 -1.38 4.89 -1.21 0.08 -2.28 -0.01 -2.59 3.13 2.97 2.99 -0.67 0.38
Nuclear Periphery 1.10 -13.68 -7.70 -8.48 4.79 0.54 -7.16 -5.98 -5.99 -2.12 1.19 -15.19 -9.65 -10.31 3.76
Nuclear Periphery Foci -0.33 -9.32 -10.47 -10.61 1.88 1.08 -5.27 -5.67 -6.10 -3.28 0.65 -10.54 -9.81 -10.20 -0.56
Nucleolus 0.83 -5.95 2.83 3.04 10.02 -0.89 -2.74 2.47 2.80 4.63 0.34 -6.73 3.47 4.04 10.97
Nucleus 5.03 -16.10 -6.32 -10.56 7.79 9.31 1.96 -10.82 -18.15 -12.79 9.62 -12.31 -12.37 -20.13 -1.99
Peroxisomes -9.97 -7.29 1.71 10.55 8.15 -4.34 -13.66 6.85 11.09 21.15* -9.61 -14.32 6.04 15.22 20.16*
Vacuole -13.67 -20.46 -29.77 -26.37 -25.07 -1.42 -14.97 -23.96 -23.11 -15.62 -9.26 -22.70 -37.87 -34.61 -29.16
Vacuole Periphery 2.95 5.61 1.18 0.27 -0.84 -5.52 1.51 -0.51 3.66 -1.38 -4.11 4.20 0.36 2.77 -1.65