Standard name | |
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Human Ortholog | |
Description | Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; sumoylated in an Mms21p-dependent manner; relative distribution to the spindle pole body decreases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 2 | 1 | 4 | 6 | 9 | 15 | 28 | 17 | – | 15 | 10 | 0 | 0 | 2 | 2 | 2 | 0 | 0 | 1 | 1 | 0 | 4 | 3 |
Bud Neck | 0 | 0 | 6 | 5 | 4 | 6 | 9 | 10 | 3 | – | 5 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 3 |
Bud Site | 0 | 1 | 8 | 16 | 6 | 16 | 34 | 16 | 18 | – | 35 | 34 | 0 | 0 | 4 | 1 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 5 | 8 | 9 | 6 | 12 | 19 | 15 | 14 | – | 12 | 4 | 18 | 40 | 56 | 22 | 16 | 29 | 0 | 0 | 1 | 1 | 1 | 1 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 1 | 0 | 2 | 3 | 7 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 23 | 21 | 5 | 3 | 24 | 7 | 20 | 7 | 2 | – | 17 | 8 | 43 | 38 | 46 | 64 | 95 | 70 | 12 | 21 | 37 | 25 | 27 | 45 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 1 | 0 | 4 |
Mitochondria | 5 | 45 | 70 | 87 | 27 | 75 | 120 | 115 | 116 | – | 98 | 138 | 17 | 17 | 62 | 29 | 12 | 43 | 0 | 3 | 4 | 5 | 5 | 11 |
Nucleus | 9 | 17 | 28 | 38 | 25 | 23 | 37 | 45 | 23 | – | 18 | 24 | 21 | 36 | 32 | 68 | 47 | 78 | 0 | 8 | 9 | 2 | 8 | 7 |
Nuclear Periphery | 1 | 5 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | – | 4 | 0 | 2 | 0 | 2 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 90 | 134 | 132 | 130 | 163 | 138 | 241 | 239 | 143 | – | 140 | 179 | 144 | 156 | 173 | 201 | 239 | 236 | 5 | 21 | 16 | 13 | 16 | 20 |
Peroxisomes | 3 | 3 | 4 | 7 | 10 | 7 | 19 | 13 | 7 | – | 14 | 11 | 5 | 4 | 7 | 5 | 6 | 4 | 1 | 0 | 1 | 3 | 3 | 7 |
SpindlePole | 98 | 80 | 188 | 184 | 238 | 213 | 337 | 345 | 182 | – | 170 | 232 | 102 | 127 | 116 | 110 | 149 | 148 | 96 | 136 | 208 | 98 | 136 | 230 |
Vac/Vac Membrane | 0 | 2 | 0 | 1 | 4 | 1 | 1 | 2 | 0 | – | 2 | 1 | 2 | 4 | 8 | 7 | 6 | 13 | 1 | 3 | 4 | 2 | 7 | 8 |
Unique Cell Count | 121 | 189 | 275 | 298 | 314 | 321 | 515 | 530 | 303 | 306 | 365 | 202 | 281 | 324 | 333 | 337 | 383 | 130 | 203 | 299 | 173 | 230 | 369 | |
Labelled Cell Count | 231 | 315 | 452 | 485 | 513 | 508 | 853 | 836 | 525 | 532 | 646 | 356 | 423 | 508 | 512 | 581 | 632 | 130 | 203 | 299 | 173 | 230 | 369 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | 4.8 | 4.1 | 3.9 | 4.3 | 3.7 | 3.7 | 3.7 | 3.4 | – | 3.7 | 3.1 | 5.2 | 5.1 | 4.6 | 7.2 | 8.4 | 8.0 | 5.4 | 4.9 | 5.5 |
Std Deviation (1e-4) | 0.8 | 0.9 | 1.2 | 1.1 | 1.0 | 0.9 | 1.3 | 1.1 | 1.2 | – | 2.9 | 0.9 | 1.2 | 1.4 | 1.5 | 1.6 | 2.0 | 2.0 | 1.5 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.06 | -0.16 | -0.15 | -0.14 | -0.26 | – | -0.15 | -0.41 | 0.34 | 0.31 | 0.15 | 0.8 | 1.04 | 0.96 | 0.38 | 0.26 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 3.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0343 | 2.2145 | 2.0832 | 2.0862 | 1.9172 | 2.107 | 2.4428 | 2.7884 | 3.057 | 2.765 | 2.738 | 2.7677 | 2.6407 | 3.146 | 3.0701 | 3.2821 | 2.3454 | 2.6937 |
Actin | 0.0035 | 0 | 0.0255 | 0.0001 | 0.0824 | 0.0034 | 0.0124 | 0.0002 | 0.0012 | 0.0109 | 0.0223 | 0.0029 | 0.0003 | 0 | 0.0016 | 0.0001 | 0.0002 | 0.0001 |
Bud | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0007 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0016 | 0.0006 | 0.0008 | 0.0003 | 0.0022 | 0.0015 | 0.0007 | 0.0004 | 0.0005 | 0.0003 | 0.001 | 0.0007 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0005 |
Bud Periphery | 0.0004 | 0 | 0 | 0 | 0.0004 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.001 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Bud Site | 0.0012 | 0.0003 | 0.0003 | 0.0001 | 0.0007 | 0.0002 | 0.0007 | 0.0004 | 0.0022 | 0.0032 | 0.0014 | 0.0001 | 0.0001 | 0.0018 | 0.0001 | 0.0002 | 0.0001 | 0 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0023 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0009 | 0.0002 | 0.0026 | 0.0024 | 0.0008 | 0.0003 | 0.0001 | 0.0015 | 0 | 0.0002 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0072 | 0.0027 | 0.0021 | 0.0104 | 0.0065 | 0.002 | 0.0155 | 0.0101 | 0.0135 | 0.0062 | 0.0075 | 0.0036 | 0.0096 | 0.0017 | 0.0006 | 0.0016 | 0.0028 | 0.0007 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0013 | 0 | 0.0001 | 0 | 0.0017 | 0.0004 | 0.0018 | 0 | 0.0007 | 0.0076 | 0.0006 | 0.0002 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Endosome | 0.0099 | 0.0001 | 0.0004 | 0.0006 | 0.0075 | 0.0016 | 0.0246 | 0.0008 | 0.0097 | 0.0175 | 0.0073 | 0.0031 | 0.0008 | 0.0003 | 0.0005 | 0.0008 | 0.0009 | 0.0002 |
Golgi | 0.0028 | 0.0001 | 0.0013 | 0.0007 | 0.009 | 0.0011 | 0.0062 | 0.0005 | 0.0014 | 0.0071 | 0.0136 | 0.0018 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0007 | 0.0002 |
Lipid Particles | 0.0133 | 0.001 | 0.0018 | 0.0085 | 0.0532 | 0.0068 | 0.0214 | 0.0028 | 0.0039 | 0.0086 | 0.027 | 0.0064 | 0.003 | 0.0033 | 0.0013 | 0.0018 | 0.0123 | 0.0013 |
Mitochondria | 0.003 | 0 | 0.0002 | 0 | 0.0066 | 0.0011 | 0.0035 | 0.0001 | 0.0002 | 0.0076 | 0.0181 | 0.0003 | 0 | 0.0002 | 0.0005 | 0 | 0.001 | 0 |
None | 0.005 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0003 | 0.0015 | 0.0001 | 0.0007 | 0.0007 | 0.0004 | 0.0002 | 0.0002 | 0.0053 | 0.0001 | 0.0002 | 0.0003 | 0 |
Nuclear Periphery | 0.022 | 0.0008 | 0.0008 | 0.0029 | 0.0094 | 0.0095 | 0.0124 | 0.0007 | 0.0075 | 0.0091 | 0.0094 | 0.0089 | 0.0014 | 0.0025 | 0.0031 | 0.0116 | 0.013 | 0.0017 |
Nucleolus | 0.0651 | 0.1187 | 0.0638 | 0.0138 | 0.069 | 0.075 | 0.0403 | 0.064 | 0.055 | 0.0466 | 0.0419 | 0.0607 | 0.0293 | 0.0633 | 0.0447 | 0.0433 | 0.0334 | 0.0485 |
Nucleus | 0.042 | 0.1637 | 0.0384 | 0.014 | 0.0058 | 0.0392 | 0.0278 | 0.1536 | 0.0523 | 0.0644 | 0.0169 | 0.0703 | 0.0109 | 0.1196 | 0.0525 | 0.0127 | 0.0067 | 0.0264 |
Peroxisomes | 0.007 | 0.0001 | 0.005 | 0.0032 | 0.0171 | 0.0013 | 0.0092 | 0.003 | 0.0025 | 0.0024 | 0.0026 | 0.0022 | 0.0034 | 0.0001 | 0.0003 | 0.0002 | 0.001 | 0.0001 |
Punctate Nuclear | 0.8095 | 0.7117 | 0.8591 | 0.945 | 0.7262 | 0.856 | 0.8184 | 0.763 | 0.8447 | 0.8006 | 0.8258 | 0.8381 | 0.9399 | 0.7989 | 0.8937 | 0.9267 | 0.9258 | 0.9202 |
Vacuole | 0.0014 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0012 | 0.0001 | 0.0008 | 0.0017 | 0.0015 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0.0004 | 0 |
Vacuole Periphery | 0.0013 | 0 | 0 | 0 | 0.0004 | 0.0003 | 0.0009 | 0 | 0.0002 | 0.0011 | 0.0008 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.9029 | 13.399 | 3.3142 | 7.0265 | 19.1845 | 18.8216 | 13.7215 | 7.2682 | 5.2625 | 18.5325 |
Translational Efficiency | 0.7173 | 0.8848 | 0.8976 | 0.5365 | 0.5865 | 0.7862 | 0.717 | 0.7016 | 0.7532 | 0.5398 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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