Standard name
Description Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; sumoylated in an Mms21p-dependent manner; relative distribution to the spindle pole body decreases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0.06 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0.05 0 0.05 0.07 0 0.06 – 0.11 0.09 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0.05 – 0 0 0.09 0.14 0.17 0.07 0.05 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.19 0.11 0 0 0.08 0 0 0 0 – 0.06 0 0.21 0.14 0.14 0.19 0.28 0.18 0.09 0.11 0.13 0.15 0.12 0.12
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.24 0.25 0.29 0.09 0.23 0.23 0.22 0.38 – 0.32 0.38 0.08 0.06 0.19 0.09 0 0.11 0 0 0 0 0 0
Nucleus 0.07 0.09 0.1 0.13 0.08 0.07 0.07 0.08 0.08 – 0.06 0.07 0.1 0.13 0.1 0.2 0.14 0.2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.74 0.71 0.48 0.44 0.52 0.43 0.47 0.45 0.47 – 0.46 0.49 0.71 0.56 0.53 0.6 0.71 0.62 0 0.1 0.05 0.08 0.07 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.81 0.42 0.68 0.62 0.76 0.66 0.65 0.65 0.6 – 0.56 0.64 0.5 0.45 0.36 0.33 0.44 0.39 0.74 0.67 0.7 0.57 0.59 0.62
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 2 1 4 6 9 15 28 17 – 15 10 0 0 2 2 2 0 0 1 1 0 4 3
Bud Neck 0 0 6 5 4 6 9 10 3 – 5 4 2 0 0 0 0 0 0 0 2 2 2 3
Bud Site 0 1 8 16 6 16 34 16 18 – 35 34 0 0 4 1 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 1 1 0 0 0 0 0 0
Cytoplasm 2 5 8 9 6 12 19 15 14 – 12 4 18 40 56 22 16 29 0 0 1 1 1 1
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 – 1 1 0 1 0 2 3 7 0 0 0 0 1 0
Endosome 23 21 5 3 24 7 20 7 2 – 17 8 43 38 46 64 95 70 12 21 37 25 27 45
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 2 1 1 0 4
Mitochondria 5 45 70 87 27 75 120 115 116 – 98 138 17 17 62 29 12 43 0 3 4 5 5 11
Nucleus 9 17 28 38 25 23 37 45 23 – 18 24 21 36 32 68 47 78 0 8 9 2 8 7
Nuclear Periphery 1 5 1 1 0 0 1 1 0 – 4 0 2 0 2 1 4 1 0 0 0 0 0 0
Nucleolus 90 134 132 130 163 138 241 239 143 – 140 179 144 156 173 201 239 236 5 21 16 13 16 20
Peroxisomes 3 3 4 7 10 7 19 13 7 – 14 11 5 4 7 5 6 4 1 0 1 3 3 7
SpindlePole 98 80 188 184 238 213 337 345 182 – 170 232 102 127 116 110 149 148 96 136 208 98 136 230
Vac/Vac Membrane 0 2 0 1 4 1 1 2 0 – 2 1 2 4 8 7 6 13 1 3 4 2 7 8
Unique Cell Count 121 189 275 298 314 321 515 530 303 306 365 202 281 324 333 337 383 130 203 299 173 230 369
Labelled Cell Count 231 315 452 485 513 508 853 836 525 532 646 356 423 508 512 581 632 130 203 299 173 230 369


Microtubule, Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 4.8 4.1 3.9 4.3 3.7 3.7 3.7 3.4 – 3.7 3.1 5.2 5.1 4.6 7.2 8.4 8.0 5.4 4.9 5.5
Std Deviation (1e-4) 0.8 0.9 1.2 1.1 1.0 0.9 1.3 1.1 1.2 – 2.9 0.9 1.2 1.4 1.5 1.6 2.0 2.0 1.5 1.4 1.5
Intensity Change (Log2) – – – -0.06 0.06 -0.16 -0.15 -0.14 -0.26 – -0.15 -0.41 0.34 0.31 0.15 0.8 1.04 0.96 0.38 0.26 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 3.1