Standard name
Human Ortholog
Description Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0.05 0.05 0.1 0.09 0.11 0.08 0.05 0.07 0.07 0.1 0.14 0.18 0.15 0.09 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.1 0 0 0.14 0 0 0 0.25 0.14 0.11 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.45 0.6 0.7 0.89 0.71 0.76 0.69 0.71 0.6 0.66 0.76 0.67 0.67 0.58 0.52 0.57 0.63 0.59 0.35 0.5 0.5 0.54 0.34 0.22
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.65 0.32 0.18 0.05 0.1 0.05 0.07 0.08 0.12 0.15 0.07 0.12 0.2 0.14 0.17 0.17 0.18 0.14 0.55 0.37 0.38 0.18 0.31 0.28
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.12 0.11 0.05 0.14 0.11 0.15 0.11 0.13 0.11 0.1 0.06 0.06 0.16 0.14 0.07 0.12 0.21 0 0.06 0.07 0.13 0.24 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 3 4 0 2 0 0 0 0 0 0 1 2 4 2 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 2 3 3 8 1 9 0 0 0 0 0 0
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 2 11 9 5 25 26 32 21 16 13 19 13 17 32 14 10 11 0 0 0 2 2 3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 5 4 0 0 0 1 1 0
Endosome 1 6 3 1 0 2 3 4 0 8 2 4 3 3 10 2 2 2 1 5 1 4 6 8
Golgi 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0
Mitochondria 11 27 0 0 14 4 12 10 64 44 22 58 0 1 0 4 3 2 0 1 0 1 1 3
Nucleus 40 163 112 154 70 192 205 204 155 205 150 187 85 73 91 54 72 71 35 162 96 79 76 34
Nuclear Periphery 0 0 0 0 0 1 1 0 1 1 0 1 0 1 1 0 0 0 0 0 0 1 2 4
Nucleolus 57 88 29 8 10 12 21 23 32 47 13 35 25 17 30 16 21 17 56 120 72 26 70 43
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 1 1 2 0 2 2 2 1 1 0 0 0 0 3 8 2 3 3 5
Vac/Vac Membrane 2 34 18 8 14 27 45 31 33 35 20 18 7 20 24 7 14 25 2 19 12 19 54 45
Unique Cell Count 88 273 161 174 98 252 295 287 260 310 198 281 126 125 174 94 115 120 102 324 192 146 226 156
Labelled Cell Count 113 321 176 180 113 265 316 309 312 370 224 335 135 133 189 97 127 132 102 324 192 146 226 156


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.3 9.5 12.8 11.5 7.0 9.0 8.4 8.9 7.5 7.5 7.8 7.2 14.2 12.0 11.5 13.3 16.1 14.9 12.6 14.0 13.6
Std Deviation (1e-4) 2.6 1.9 3.0 2.4 2.0 2.7 2.4 2.1 2.0 2.0 1.8 1.8 4.3 4.0 4.2 4.0 4.8 5.2 3.9 4.0 3.5
Intensity Change (Log2) -0.16 -0.88 -0.51 -0.61 -0.53 -0.77 -0.77 -0.73 -0.83 0.14 -0.1 -0.16 0.05 0.33 0.22 -0.03 0.12 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 -0.6 1.1 0.7 1.5 0.5 -0.7 -0.1 0 1.1 1.9 3.2 2.1 0.6 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 1.8 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 4.9 0 0 0 6.8 5.0 4.4 6.2 0 0 0 0 0 0
Nucleus 4.3 0.3 1.5 0 0.3 -2.1 -0.8 1.3 -0.7 -0.4 -2.0 -3.2 -2.0 -1.2 -1.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -3.9 -1.7 -4.4 -3.6 -3.2 -1.6 -0.8 -3.4 -1.6 0.4 -1.0 -0.2 -0.2 0.1 -0.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.2 0.7 -0.1 1.2 -0.1 0.5 0 -0.3 -1.8 -1.7 1.2 0.7 -1.0 0.3 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.7564 8.5559 7.5916 6.7489 7.0445 7.36 12.8937 14.1821 13.8088 13.4395 12.5359 13.4745 9.9465 10.8991 10.2399 8.8909 10.1809 10.2744
Actin 0.0026 0 0 0 0.0001 0 0.0047 0 0.002 0.0322 0 0.0026 0.0133 0 0.0001 0.0268 0.013 0
Bud 0.0001 0 0 0.0001 0.0001 0.0001 0.0003 0 0.0004 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001
Bud Neck 0.0004 0.0004 0.0004 0.0008 0.0024 0.0022 0.0013 0.0006 0.0012 0.0005 0.0011 0.0016 0.0011 0.0005 0.0007 0.0055 0.0009 0.0025
Bud Periphery 0.0002 0.0001 0.0001 0.0002 0.0003 0.0002 0.0007 0.0001 0.0005 0.0007 0.0005 0.0001 0.0002 0.0001 0.0001 0.0003 0.0002 0.0002
Bud Site 0.001 0.0002 0.0002 0.0003 0.0005 0.0003 0.0012 0.0002 0.0016 0.0007 0.0004 0.0002 0.0012 0.0004 0.0003 0.0019 0.0005 0.0002
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0003 0.0001 0.0003 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001 0.0002
Cytoplasm 0 0.0002 0 0 0.0002 0 0.005 0 0.0004 0.0002 0.0001 0.0007 0.0026 0 0.0001 0.0101 0.0001 0
Cytoplasmic Foci 0.0002 0 0.0001 0 0.0001 0.0002 0.01 0 0.0038 0.0047 0.0105 0.0026 0.0049 0 0.0003 0.0046 0.0018 0.0003
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0007 0.0001 0 0.0001 0.0001 0 0 0.0003 0 0
Endoplasmic Reticulum 0 0 0 0 0.0001 0 0.0026 0 0.0004 0.0002 0.0001 0.0003 0.0012 0 0.0001 0.0016 0 0
Endosome 0.0003 0 0 0.0001 0.0004 0.0001 0.0203 0 0.0028 0.0039 0.0108 0.0036 0.008 0.0001 0.0028 0.0109 0.001 0.0005
Golgi 0.0003 0 0 0 0.0001 0 0.0034 0 0.0016 0.0099 0.0018 0.0012 0.0016 0 0.0006 0.003 0.0042 0.0005
Lipid Particles 0.0005 0 0.0002 0.0001 0.0003 0.0009 0.014 0 0.0058 0.0109 0.0076 0.0039 0.0059 0 0.0009 0.0036 0.0012 0.0035
Mitochondria 0.0013 0.0001 0.0001 0.0001 0.0003 0.0001 0.01 0.0001 0.0021 0.0039 0.0027 0.0003 0.0008 0.0001 0.001 0.0015 0.0019 0.0002
None 0 0 0 0 0 0 0.0058 0 0.0034 0.0002 0.0001 0.0019 0.0027 0 0 0.0205 0 0
Nuclear Periphery 0.0005 0.0034 0.0006 0.0004 0.0041 0.0005 0.0092 0.002 0.002 0.0131 0.001 0.0022 0.0054 0.0003 0.0017 0.006 0.0004 0.0002
Nucleolus 0.4181 0.436 0.4996 0.5179 0.3348 0.4748 0.4401 0.4627 0.5341 0.3926 0.4479 0.5164 0.4566 0.4104 0.5402 0.3806 0.4753 0.5187
Nucleus 0.5689 0.558 0.4969 0.4778 0.6519 0.5172 0.4535 0.5317 0.4303 0.5149 0.4974 0.457 0.4767 0.5859 0.4437 0.5082 0.4797 0.4701
Peroxisomes 0.0013 0 0 0 0 0 0.0022 0 0.0022 0.0079 0.0015 0.0011 0.0039 0 0.0001 0.0062 0.0058 0.0001
Punctate Nuclear 0.0036 0.001 0.0014 0.0013 0.0027 0.0024 0.0079 0.002 0.0021 0.002 0.011 0.003 0.0121 0.0004 0.0061 0.0069 0.0133 0.002
Vacuole 0.0004 0.0004 0.0003 0.0006 0.0011 0.0007 0.0046 0.0003 0.0017 0.0005 0.0034 0.0008 0.0011 0.0013 0.0008 0.0006 0.0003 0.0006
Vacuole Periphery 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0028 0.0001 0.0005 0.0003 0.0016 0.0002 0.0005 0.0001 0.0003 0.0004 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.6237 31.4383 33.0488 32.5829 35.1478 13.1808 30.7939 29.0526 21.4646 35.2131
Translational Efficiency 1.3769 1.9327 1.4994 1.3061 1.4675 3.9774 1.7077 1.7971 2.0508 1.4642

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1954 1264 2681 1630 1512 2124 2033 1510 3466 3388 4714 3140

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1017.78 1247.92 1364.27 1566.43 1081.69 1254.86 1311.68 1440.30 1045.66 1252.27 1341.59 1505.78
Standard Deviation 172.22 271.86 224.63 246.61 179.13 249.18 219.43 261.60 178.11 257.90 223.92 261.63
Intensity Change Log 2 0.294100 0.422703 0.622055 0.214239 0.278129 0.413082 0.253506 0.350026 0.518170

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000336 0.000888 0.000446 0.001308 0.000436 0.000452 0.000500 0.000701 0.000380 0.000615 0.000469 0.001016
Bud Neck 0.004259 0.011007 0.022673 0.064721 0.008534 0.013821 0.017549 0.027395 0.006124 0.012771 0.020463 0.046771
Bud Site 0.000391 0.001376 0.000974 0.006229 0.000893 0.002094 0.001517 0.001767 0.000610 0.001826 0.001208 0.004083
Cell Periphery 0.000038 0.000098 0.000034 0.000150 0.000059 0.000118 0.000053 0.000066 0.000047 0.000111 0.000042 0.000109
Cytoplasm 0.000803 0.003378 0.000313 0.002768 0.004068 0.002014 0.001533 0.002561 0.002227 0.002523 0.000839 0.002669
Cytoplasmic Foci 0.000127 0.000717 0.000042 0.002043 0.000520 0.000384 0.000092 0.000377 0.000298 0.000509 0.000064 0.001242
Eisosomes 0.000032 0.000047 0.000040 0.000038 0.000038 0.000044 0.000051 0.000044 0.000035 0.000045 0.000044 0.000041
Endoplasmic Reticulum 0.001791 0.001535 0.000729 0.001530 0.002039 0.002050 0.001222 0.000972 0.001899 0.001858 0.000942 0.001262
Endosome 0.000263 0.000619 0.000153 0.004030 0.000409 0.000970 0.000222 0.000396 0.000326 0.000839 0.000183 0.002282
Golgi 0.000247 0.000565 0.000166 0.003417 0.000302 0.000348 0.000173 0.000385 0.000271 0.000429 0.000169 0.001959
Lipid Particles 0.000093 0.000350 0.000020 0.000656 0.000131 0.000187 0.000035 0.000070 0.000109 0.000248 0.000026 0.000374
Mitochondria 0.001252 0.001471 0.001157 0.006556 0.000560 0.001493 0.001048 0.002085 0.000950 0.001485 0.001110 0.004406
Mitotic Spindle 0.002312 0.003569 0.001014 0.034577 0.001092 0.002858 0.002176 0.007262 0.001780 0.003124 0.001515 0.021441
None 0.001334 0.004030 0.000648 0.002235 0.002087 0.002284 0.003044 0.002413 0.001663 0.002935 0.001681 0.002321
Nuclear Periphery 0.000238 0.000242 0.000469 0.005368 0.000185 0.000695 0.000246 0.000906 0.000215 0.000526 0.000373 0.003222
Nuclear Periphery Foci 0.002950 0.001858 0.000251 0.003860 0.002497 0.001412 0.000210 0.000753 0.002753 0.001579 0.000233 0.002366
Nucleolus 0.416655* 0.373455* 0.346473* 0.305836* 0.356233* 0.351697* 0.358996* 0.387791* 0.390297* 0.359814* 0.351874* 0.345247*
Nucleus 0.565386* 0.590838* 0.622603* 0.537552* 0.617714* 0.614345* 0.608952* 0.559749* 0.588214* 0.605575* 0.616716* 0.548226*
Peroxisomes 0.000872 0.002044 0.000888 0.001260 0.001155 0.001100 0.001538 0.001624 0.000996 0.001452 0.001168 0.001435
Vacuole 0.000310 0.001119 0.000384 0.007494 0.000731 0.000863 0.000504 0.001468 0.000493 0.000958 0.000436 0.004596
Vacuole Periphery 0.000311 0.000796 0.000522 0.008372 0.000317 0.000770 0.000340 0.001214 0.000313 0.000780 0.000444 0.004930

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.09 -5.65 -5.42 -1.11 -4.51 -0.20 -3.80 -11.17 -6.85 -8.86 -2.23 -6.76 -7.76 -3.32 -6.28
Bud Neck -9.74 -25.36 -32.27 -28.83 -22.86 -6.01 -12.74 -17.70 -14.09 -9.94 -11.70 -27.49 -35.56 -30.49 -23.65
Bud Site -5.85 -11.60 -11.61 -9.25 -10.11 -3.33 -2.65 -8.30 -2.31 -3.47 -5.54 -5.88 -13.93 -8.37 -10.65
Cell Periphery -4.93 0.11 -6.24 -1.92 -6.33 -2.28 0.56 -1.37 1.78 -3.08 -3.98 0.55 -6.15 0.22 -7.14
Cytoplasm -2.63 1.20 -4.58 -0.43 -6.40 1.98 2.33 1.22 -1.29 -1.94 -0.15 2.62 -1.43 -1.48 -5.95
Cytoplasmic Foci -2.22 2.50 -3.43 -1.03 -3.73 0.68 2.27 0.93 0.46 -1.25 -1.40 2.73 -2.16 -1.01 -3.88
Eisosomes -8.09 -5.60 -3.77 4.58 1.07 -2.95 -6.92 -2.37 0.83 4.41 -7.84 -8.78 -4.60 3.68 3.49
Endoplasmic Reticulum 1.68 8.74 0.93 -0.99 -10.63 0.03 6.67 6.57 4.82 -0.17 0.34 11.02 4.65 3.48 -7.74
Endosome -2.26 1.49 -4.58 -3.41 -4.82 -2.95 1.99 0.17 2.93 -1.54 -3.99 2.47 -4.38 -1.69 -5.06
Golgi -1.97 2.32 -2.93 -2.21 -3.06 -0.64 4.01 -0.86 -0.44 -1.85 -2.13 4.29 -3.05 -2.40 -3.38
Lipid Particles -1.15 3.37 -1.90 -0.17 -2.38 -0.81 2.59 1.09 1.79 -3.04 -1.50 4.08 -1.62 -0.01 -2.70
Mitochondria -0.33 -0.14 -6.66 -6.40 -8.46 -3.61 -10.81 -16.91 -3.92 -11.17 -1.55 -1.18 -8.78 -7.71 -10.51
Mitotic Spindle -0.83 1.11 -8.33 -7.57 -8.90 -2.10 -2.29 -5.49 -4.52 -4.33 -1.72 -0.65 -9.89 -8.99 -9.88
None -2.87 2.82 -5.12 1.03 -8.83 -0.29 -1.09 -1.43 -1.01 0.07 -2.69 0.07 -4.33 -0.04 -3.67
Nuclear Periphery -0.20 -3.39 -8.68 -8.67 -8.03 -3.10 -2.27 -7.59 -2.11 -6.61 -3.00 -3.99 -10.20 -8.25 -9.31
Nuclear Periphery Foci 1.32 4.43 1.14 -0.28 -3.71 2.08 5.10 3.42 1.34 -2.29 2.46 6.37 2.70 0.22 -4.34
Nucleolus 4.43 8.56 16.73 10.15 9.29 0.57 -0.03 0.58 0.11 0.60 4.90 6.31 12.17 7.54 6.61
Nucleus -2.58 -6.36 0.41 2.72 6.03 0.30 1.16 4.15 4.19 3.10 -2.81 -3.94 2.91 5.42 6.51
Peroxisomes -6.84 -0.67 -3.82 4.22 -3.48 0.44 -4.02 -4.31 -3.43 -0.63 -3.85 -3.15 -5.88 -0.23 -3.22
Vacuole -4.49 -1.90 -10.63 -9.09 -10.38 -0.37 1.44 -3.76 -3.59 -5.19 -3.17 0.41 -11.23 -9.76 -11.51
Vacuole Periphery -3.60 -2.99 -5.13 -4.46 -4.86 -2.58 -1.11 -7.99 -1.87 -7.74 -3.87 -3.20 -6.00 -4.73 -5.67