| Standard name | |
|---|---|
| Description | Subunit of a TORC1-stimulating GTPase and the EGO/GSE complex; subunit of Gtr1-Gtr2, a GTPase that activates TORC1 in response to amino acid stimulation; negatively regulates the GTPase cycle of Gtr1p, a Ran/TC4 homolog; subunit of EGO, a vacuolar/endosomal membrane complex that regulates exit from rapamycin-induced growth arrest and endosome to plasma membrane sorting of Gap1p; activates transcription when chromatin bound; activated by the Lst4p-Lst7p GAP complex; homolog of human RagC |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
| Bud | 1 | 0 | – | – | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 3 | 3 | 5 |
| Bud Neck | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Bud Site | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 0 | 0 | – | – | 0 | 1 | 0 | – | – | – | – | – | 0 | 1 | 0 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | 3 | 19 | – | – | 5 | 2 | 2 | – | – | – | – | – | 15 | 19 | 21 | 18 | 14 | 22 | 0 | 0 | 0 | 2 | 1 | 0 |
| Endoplasmic Reticulum | 0 | 1 | – | – | 0 | 2 | 0 | – | – | – | – | – | 1 | 0 | 3 | 16 | 6 | 19 | 0 | 2 | 0 | 1 | 1 | 1 |
| Endosome | 0 | 1 | – | – | 1 | 2 | 0 | – | – | – | – | – | 15 | 5 | 12 | 2 | 0 | 7 | 7 | 3 | 0 | 18 | 17 | 31 |
| Golgi | 0 | 2 | – | – | 1 | 2 | 0 | – | – | – | – | – | 2 | 5 | 8 | 24 | 13 | 23 | 1 | 1 | 0 | 1 | 2 | 3 |
| Mitochondria | 32 | 5 | – | – | 0 | 10 | 5 | – | – | – | – | – | 6 | 1 | 1 | 10 | 4 | 17 | 3 | 3 | 0 | 7 | 2 | 5 |
| Nucleus | 14 | 17 | – | – | 0 | 1 | 0 | – | – | – | – | – | 28 | 10 | 17 | 18 | 5 | 17 | 2 | 10 | 1 | 8 | 11 | 16 |
| Nuclear Periphery | 34 | 43 | – | – | 1 | 4 | 3 | – | – | – | – | – | 37 | 95 | 90 | 80 | 23 | 100 | 13 | 13 | 2 | 44 | 53 | 53 |
| Nucleolus | 17 | 2 | – | – | 0 | 1 | 0 | – | – | – | – | – | 9 | 1 | 3 | 10 | 3 | 12 | 1 | 1 | 0 | 2 | 4 | 6 |
| Peroxisomes | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 0 | 0 | – | – | 1 | 1 | 0 | – | – | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
| Vac/Vac Membrane | 125 | 255 | – | – | 12 | 23 | 9 | – | – | – | – | – | 394 | 462 | 464 | 296 | 132 | 319 | 143 | 264 | 8 | 399 | 336 | 447 |
| Unique Cell Count | 168 | 297 | 18 | 35 | 16 | 442 | 502 | 515 | 345 | 157 | 380 | 174 | 309 | 15 | 490 | 442 | 582 | |||||||
| Labelled Cell Count | 226 | 345 | 21 | 49 | 19 | 507 | 599 | 621 | 478 | 201 | 538 | 174 | 309 | 15 | 490 | 442 | 582 | |||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 7.8 | 11.0 | – | – | 9.6 | 7.9 | 7.5 | – | – | – | – | – | 10.1 | 10.9 | 11.0 | 14.3 | 13.9 | 14.0 | 10.5 | 10.6 | 11.1 |
| Std Deviation (1e-4) | 1.4 | 2.7 | – | – | 1.6 | 1.7 | 2.3 | – | – | – | – | – | 2.2 | 2.1 | 1.9 | 4.0 | 3.8 | 3.6 | 2.2 | 2.2 | 2.6 |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
