Standard name
Human Ortholog
Description Alcohol acetyltransferase; may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Cytoplasm 0.82 0.88 0.93 0.91 0.86 0.77 0.69 0.76 0.75 0.76 0.62 0.68 0.97 0.98 0.96 0.73 0.69 0.75 0.42 0.62 0.53 0.69 0.72 0.65
Endoplasmic Reticulum 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0.21 0.13 0.1 0.1 0 0.05
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.1 0.13 0.14 0.38 0.45 0.38 0.47 0.44 0.49 0.53 0 0 0 0.07 0 0 0.22 0.11 0.21 0.08 0.08 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.17 0.24 0.1 0.06 0.05 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 6 1 0 1 5 5 0 4 5
Bud 1 0 1 5 1 5 8 10 6 17 3 6 1 1 0 2 0 0 4 3 5 1 5 8
Bud Neck 0 1 1 0 1 1 3 2 1 5 0 0 0 0 0 2 0 0 0 4 5 1 2 3
Bud Site 1 0 0 0 0 1 3 0 4 3 1 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 4 2 2 4 0 1 0 1 1 1 1 1 2 1 10 3 8 0 2 5 2 2 2
Cytoplasm 65 212 278 174 230 274 352 419 258 388 95 144 180 234 226 217 116 80 63 163 160 82 133 118
Endoplasmic Reticulum 0 19 2 0 2 1 0 4 0 1 0 1 2 2 3 14 8 5 31 35 29 11 6 8
Endosome 4 0 0 1 5 6 5 3 1 0 1 1 1 0 1 12 12 2 1 2 4 3 1 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 0 0 0 0 0 0 0
Mitochondria 4 9 30 25 38 136 227 209 160 222 75 113 1 2 0 21 2 2 32 27 64 10 15 14
Nucleus 0 1 0 0 0 2 1 1 1 1 0 0 1 1 1 5 1 4 0 0 0 0 0 0
Nuclear Periphery 0 2 0 0 0 0 1 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 1 0
Nucleolus 1 0 0 2 0 1 2 0 1 0 0 1 1 0 0 1 0 0 0 0 0 0 0 1
Peroxisomes 1 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 3 0 0 1 3 2 11 9 7 2 5 2 0 0 0 1 0 0 2 1 2 1 3 3
Vac/Vac Membrane 3 6 3 1 9 9 8 2 2 1 2 0 1 0 4 51 40 11 8 14 11 1 3 8
Unique Cell Count 79 241 299 191 266 356 508 553 342 509 154 212 185 238 235 296 169 106 152 263 303 119 185 183
Labelled Cell Count 86 255 317 212 294 438 622 660 443 642 184 272 189 242 237 345 185 112 152 263 303 119 185 183


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.4 3.8 3.9 3.8 3.3 2.7 3.2 3.0 2.6 3.0 2.5 4.9 4.5 4.8 6.6 7.6 8.0 4.4 4.5 5.7
Std Deviation (1e-4) 0.7 0.8 1.1 1.2 1.3 1.5 0.9 1.1 1.4 0.9 2.1 0.8 2.2 1.5 2.0 1.5 1.8 1.6 1.7 1.8 2.5
Intensity Change (Log2) – – – 0.01 -0.02 -0.21 -0.51 -0.27 -0.33 -0.56 -0.37 -0.6 0.36 0.23 0.33 0.79 1.0 1.07 0.21 0.22 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 6.9 8.2 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0226 -0.0968 -0.0644 -0.3793 -0.745 0.1087 1.2009 0.6209 1.3377 1.0387 1.2596 1.0476 1.5045 1.8542 1.8692 1.6522 2.003 1.7318
Actin 0.031 0.0001 0.0165 0.0075 0.0003 0.0024 0.0133 0.0007 0.0054 0.0263 0.0137 0.008 0.0303 0.0012 0.0268 0.0003 0.0288 0.0013
Bud 0.0004 0.0001 0.0005 0.0002 0.0001 0.0003 0.0008 0.0007 0.0003 0.0008 0.0003 0.0003 0.0007 0.0015 0.0012 0.0002 0.0021 0.0002
Bud Neck 0.0015 0.0001 0.0079 0.0021 0.0001 0.0004 0.0148 0.0001 0.0002 0.0005 0.0009 0.0005 0.0015 0.0001 0.0011 0.0001 0.0062 0.0013
Bud Periphery 0.0006 0.0001 0.0009 0.0004 0.0002 0.0015 0.002 0.0018 0.0008 0.0009 0.0003 0.0012 0.001 0.0006 0.0047 0.0003 0.0081 0.0003
Bud Site 0.0014 0.0015 0.0116 0.0027 0 0.0002 0.0128 0.0034 0.0007 0.014 0.0025 0.0001 0.0095 0.0062 0.0079 0.004 0.0047 0.0003
Cell Periphery 0.0005 0.0009 0.0012 0.0002 0.0001 0.0002 0.0012 0.0004 0.0003 0.0002 0.0001 0.0001 0.0003 0.0003 0.0005 0.0001 0.0012 0.0001
Cytoplasm 0.1227 0.1541 0.1973 0.1434 0.0272 0.2571 0.029 0.079 0.0888 0.0354 0.0603 0.1442 0.0648 0.1094 0.1387 0.0903 0.098 0.1191
Cytoplasmic Foci 0.0191 0.0038 0.0122 0.031 0.023 0.0107 0.054 0.0152 0.0145 0.0457 0.0935 0.0119 0.0282 0.0038 0.0078 0.021 0.0089 0.0182
Eisosomes 0.0005 0.0002 0.0013 0.0007 0.0001 0.0001 0.0004 0.0005 0.0006 0.0004 0.0001 0.0002 0.0006 0.0002 0.0006 0.0001 0.0006 0.0001
Endoplasmic Reticulum 0.0067 0.0184 0.0076 0.0047 0.0006 0.0089 0.0027 0.017 0.0057 0.0007 0.0008 0.0223 0.0167 0.0305 0.0043 0.0018 0.0006 0.0028
Endosome 0.0114 0.0005 0.0174 0.0024 0.0053 0.005 0.0213 0.0067 0.022 0.0091 0.0321 0.0065 0.0122 0.0012 0.0067 0.0133 0.0029 0.0022
Golgi 0.0043 0.0001 0.0057 0.0005 0.0006 0.0006 0.0116 0.0009 0.001 0.0047 0.0387 0.0008 0.0054 0.0001 0.003 0.0013 0.0017 0.0004
Lipid Particles 0.0211 0.0008 0.0093 0.0017 0.0161 0.0011 0.045 0.0032 0.0032 0.0065 0.0758 0.0022 0.0069 0.0004 0.0019 0.0031 0.0039 0.0016
Mitochondria 0.002 0.0002 0.0097 0.0009 0.0043 0.0063 0.0046 0.0004 0.0014 0.005 0.0106 0.0024 0.0018 0.0002 0.0019 0.0007 0.0218 0.0009
None 0.7404 0.8163 0.6844 0.7858 0.9108 0.6894 0.6644 0.8634 0.834 0.8361 0.6012 0.7892 0.8 0.8379 0.7838 0.8563 0.7748 0.8376
Nuclear Periphery 0.0108 0.0004 0.0013 0.0008 0.0013 0.0058 0.0197 0.0011 0.0056 0.0007 0.0004 0.0016 0.0033 0.0012 0.0013 0.0005 0.0003 0.0023
Nucleolus 0.0005 0.0002 0.0016 0.0004 0.0004 0.0003 0.0014 0.0002 0.0007 0.0003 0.0007 0.0001 0.0006 0.0003 0.0003 0.0003 0.0047 0.0003
Nucleus 0.0034 0.0005 0.0018 0.0013 0.0004 0.0016 0.028 0.0007 0.003 0.0004 0.0004 0.0007 0.0016 0.0014 0.0007 0.0005 0.0029 0.0006
Peroxisomes 0.0121 0 0.0034 0.0028 0.0049 0.0003 0.0609 0.0024 0.0037 0.0103 0.0612 0.0057 0.008 0.0002 0.0047 0.0011 0.0233 0.0074
Punctate Nuclear 0.0047 0.0006 0.0018 0.0094 0.0025 0.0044 0.0063 0.0009 0.004 0.0011 0.004 0.0007 0.0045 0.002 0.0007 0.0014 0.0008 0.0021
Vacuole 0.0031 0.0011 0.0058 0.0012 0.0011 0.0023 0.0037 0.0012 0.003 0.0006 0.0019 0.0011 0.0016 0.0012 0.0011 0.0029 0.0034 0.0006
Vacuole Periphery 0.002 0 0.0008 0.0001 0.0006 0.001 0.002 0.0002 0.001 0.0003 0.0005 0.0003 0.0006 0.0001 0.0003 0.0004 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.5482 15.9489 26.4006 21.6079 11.5659 7.544 19.6922 22.1864 15.6236 10.835
Translational Efficiency 0.8583 1.3245 1.0542 1.123 0.7314 0.7032 0.7399 0.7821 0.9061 0.5989

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alcohol acetyltransferase; may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking
Localization
Cell Percentages cytoplasm (21%), ER (17%), mixed (37%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Atf2

Atf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available