Standard name
Human Ortholog
Description Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 0.98 0.9 0.9 0.81 0.72 0.7 0.72 0.63 0.67 0.7 1.0 0.98 0.98 0.94 0.92 0.97 0.96 0.95 0.93 0.94 0.92 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.05 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.07 0.08 0.14 0.18 0.19 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.12 0.11 0.15 0.18 0.21 0.12 0.16 0.14 0.15 0 0 0.05 0.06 0.08 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 1 1 1 0 0 0 0 1 0 0 0 0 1 0 0 1
Bud 0 0 1 0 0 2 2 3 2 1 0 3 0 0 0 2 0 0 1 0 0 1 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 0 5 3 1 3 10 8 11 13 15 20 0 0 1 9 10 8 0 0 1 0 0 0
Cytoplasm 280 250 354 195 283 261 285 296 224 210 213 217 225 418 363 378 261 272 267 236 337 170 315 261
Endoplasmic Reticulum 0 0 1 2 5 2 11 20 7 25 8 12 1 2 0 17 5 6 1 0 5 0 1 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 5 7
Golgi 0 0 0 0 0 0 0 1 0 1 0 0 0 1 1 1 1 1 0 0 1 0 3 7
Mitochondria 0 0 1 0 1 15 28 34 43 60 60 46 0 0 0 1 0 2 0 0 3 0 1 0
Nucleus 0 0 0 0 2 3 7 4 5 3 3 4 0 3 3 1 3 2 1 1 1 1 5 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 1 0 0 1 0 0 1
Nucleolus 0 0 1 1 0 1 2 0 1 0 2 0 0 0 0 2 1 0 0 0 1 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 1 0 6 27 34 48 73 89 38 53 43 48 1 19 19 24 24 1 2 4 2 3 3 6
Unique Cell Count 281 250 362 217 314 324 396 425 313 333 317 312 226 426 371 403 284 280 279 248 363 181 344 303
Labelled Cell Count 282 250 369 228 326 335 419 455 333 368 345 350 227 443 387 436 306 293 279 248 363 181 344 303


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.2 28.6 22.0 20.3 16.2 13.4 12.4 12.0 10.7 10.4 9.8 10.4 24.2 22.7 21.0 24.5 21.0 24.7 25.9 28.3 29.5
Std Deviation (1e-4) 4.1 6.1 3.9 3.6 2.9 2.7 2.6 2.7 2.3 2.3 2.4 2.5 4.2 4.4 3.6 7.1 5.9 6.7 5.9 6.1 6.3
Intensity Change (Log2) -0.11 -0.44 -0.72 -0.82 -0.87 -1.03 -1.07 -1.16 -1.08 0.14 0.05 -0.06 0.16 -0.06 0.17 0.24 0.37 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.1 0.5 1.8 2.1 2.6 3.4 0 0 0 0.9 1.8 1.3
Cytoplasm -4.2 -4.3 -7.4 -9.8 -10.4 -9.7 -11.7 -10.7 -10.2 1.7 0.3 0 -2.7 -3.5 -0.5
Endoplasmic Reticulum 0 0 0 2.8 3.8 0 5.0 0 3.4 0 0 0 3.6 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 4.9 5.2 7.1 8.3 8.5 7.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 5.4 5.0 6.4 7.6 8.3 5.5 6.7 6.0 6.5 0 2.2 2.6 3.1 4.1 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.8456 27.5815 25.7197 24.1322 24.3338 25.1754 23.7737 29.7194 26.252 23.5979 20.9043 26.0823 22.0801 28.9833 26.2432 22.3652 21.7157 25.1952
Actin 0.0005 0.0001 0.0009 0.0001 0 0.0003 0.0072 0.0001 0.0144 0.0019 0.0155 0.0029 0.0273 0.0001 0.0024 0.011 0.0075 0.0007
Bud 0.0001 0.0002 0 0 0.0002 0.0001 0.0011 0.0001 0.0008 0.0002 0.001 0.0002 0.0002 0 0.0001 0.0005 0.0004 0.0009
Bud Neck 0.0003 0.0001 0.0003 0.0004 0.0004 0.0005 0.001 0.0002 0.0031 0.0003 0.0012 0.0009 0.0002 0.0001 0.0002 0.0004 0.0007 0.0009
Bud Periphery 0 0 0 0 0 0.0001 0.0021 0 0.0007 0.0004 0.0017 0.0001 0.0002 0 0.0001 0.0006 0.0002 0.0005
Bud Site 0.0001 0.0003 0.0002 0.0001 0 0.0001 0.0016 0.0001 0.0024 0.0002 0.002 0.0007 0.0008 0 0.0003 0.0008 0.003 0.0002
Cell Periphery 0.0001 0 0 0 0 0 0.0003 0 0.0002 0.0001 0.001 0 0.0001 0 0 0.0002 0.0001 0
Cytoplasm 0.9584 0.9661 0.9633 0.9653 0.9484 0.9516 0.8888 0.9842 0.9223 0.9122 0.8022 0.9295 0.8749 0.99 0.9729 0.8566 0.8401 0.9696
Cytoplasmic Foci 0.0012 0.0006 0.0009 0.0014 0.0007 0.001 0.008 0.0005 0.0055 0.0038 0.0077 0.0088 0.0132 0.0002 0.0012 0.0133 0.038 0.001
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0002 0.0001 0.0004 0.0001 0.0002 0 0 0.0002 0.0001 0
Endoplasmic Reticulum 0.0027 0.006 0.0008 0.0017 0.0017 0.0027 0.0053 0.0005 0.0014 0.0029 0.0166 0.0028 0.0064 0.0002 0.001 0.0051 0.007 0.0009
Endosome 0.0012 0.0003 0.0012 0.0015 0.0016 0.0025 0.0135 0.0002 0.0044 0.0073 0.0286 0.0089 0.0091 0 0.003 0.0268 0.0258 0.0007
Golgi 0.0001 0 0.0003 0.0001 0 0.0002 0.0026 0 0.0018 0.0016 0.0107 0.0027 0.0045 0 0.0014 0.0103 0.0041 0.0001
Lipid Particles 0.0003 0 0.001 0 0 0.0003 0.0024 0.0001 0.0051 0.0037 0.0242 0.0038 0.0094 0 0.0019 0.0173 0.0088 0
Mitochondria 0.0002 0 0.0002 0.0001 0.0001 0.0023 0.0049 0.0001 0.0049 0.0205 0.027 0.0018 0.0022 0 0.001 0.027 0.0012 0.0014
None 0.0011 0.0005 0.0005 0.0004 0.0006 0.0006 0.0022 0.0003 0.001 0.002 0.0102 0.0007 0.0018 0.0002 0.0004 0.0018 0.0219 0.0004
Nuclear Periphery 0.0063 0.0021 0.0034 0.0029 0.0065 0.0063 0.011 0.0016 0.0027 0.0118 0.0151 0.007 0.0118 0.0007 0.0032 0.0052 0.0056 0.0029
Nucleolus 0.0002 0 0.0001 0 0.0001 0.0002 0.0004 0 0.0002 0.0004 0.0005 0.0001 0.0001 0 0.0002 0.0002 0.0004 0
Nucleus 0.0238 0.023 0.0253 0.0248 0.0344 0.0265 0.0362 0.0114 0.0209 0.0234 0.0187 0.0248 0.0228 0.0084 0.0076 0.0153 0.0119 0.0186
Peroxisomes 0 0 0.0003 0 0 0 0.0013 0 0.0024 0.0005 0.0016 0.0011 0.0085 0 0.0004 0.002 0.0096 0.0001
Punctate Nuclear 0.0011 0.0002 0.0008 0.0004 0.0011 0.0025 0.0026 0.0002 0.0015 0.0008 0.0013 0.0013 0.0039 0.0001 0.0006 0.0016 0.0116 0.0002
Vacuole 0.002 0.0003 0.0004 0.0006 0.0032 0.0014 0.0061 0.0003 0.0032 0.0022 0.0096 0.001 0.0012 0 0.0014 0.0022 0.0014 0.0007
Vacuole Periphery 0.0003 0 0.0001 0.0001 0.0009 0.0006 0.0016 0 0.0009 0.0036 0.0032 0.0006 0.0013 0 0.0007 0.0016 0.0007 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.554 37.4573 35.2069 37.3938 43.4616 26.0576 23.1446 22.4375 29.5029 33.9104
Translational Efficiency 3.8152 1.9412 2.1672 2.1125 1.6321 2.0626 2.4061 2.0054 1.5608 2.0005

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1715 127 2301 1227 1516 1696 243 1360 3231 1823 2544 2587

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1895.25 2313.38 2256.25 2365.39 1834.12 2055.50 2503.15 2354.18 1866.57 2073.47 2279.83 2359.50
Standard Deviation 318.29 380.00 368.66 421.16 306.99 345.32 370.10 428.72 314.52 353.99 375.87 425.19
Intensity Change Log 2 0.287614 0.251539 0.319690 0.164401 0.448657 0.360137 0.228332 0.351848 0.339723

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000120 0.000430 0.000103 0.001404 0.000181 0.000196 0.001098 0.000785 0.000149 0.000213 0.000198 0.001078
Bud Neck 0.000254 0.001073 0.000580 0.001622 0.000411 0.000422 0.013514 0.001855 0.000327 0.000468 0.001816 0.001744
Bud Site 0.000994 0.005946 0.002321 0.010617 0.001648 0.002151 0.016820 0.009806 0.001301 0.002415 0.003706 0.010191
Cell Periphery 0.000110 0.000506 0.000138 0.001437 0.000180 0.000103 0.001403 0.001125 0.000143 0.000131 0.000259 0.001273
Cytoplasm 0.905489* 0.835122* 0.929912* 0.874736* 0.855615* 0.873036* 0.607386* 0.867741* 0.882088* 0.870395* 0.899105* 0.871058*
Cytoplasmic Foci 0.005751 0.035658 0.005602 0.007703 0.006719 0.005115 0.039285 0.009486 0.006205 0.007243 0.008819 0.008640
Eisosomes 0.000001 0.000002 0.000001 0.000003 0.000001 0.000001 0.000009 0.000003 0.000001 0.000001 0.000001 0.000003
Endoplasmic Reticulum 0.000193 0.000099 0.000052 0.000112 0.000439 0.000303 0.000603 0.000111 0.000308 0.000289 0.000104 0.000111
Endosome 0.000224 0.000555 0.000484 0.000885 0.000361 0.000568 0.007739 0.002301 0.000289 0.000568 0.001177 0.001629
Golgi 0.000908 0.002601 0.001677 0.004594 0.001245 0.001150 0.006444 0.004731 0.001066 0.001251 0.002132 0.004666
Lipid Particles 0.000634 0.002576 0.001360 0.006734 0.000422 0.000298 0.013728 0.004837 0.000535 0.000457 0.002542 0.005737
Mitochondria 0.000079 0.000045 0.000052 0.000117 0.000165 0.000178 0.000112 0.000325 0.000120 0.000169 0.000058 0.000226
Mitotic Spindle 0.000972 0.001760 0.001697 0.005448 0.001978 0.004602 0.014968 0.009428 0.001444 0.004404 0.002965 0.007540
None 0.006000 0.008277 0.001624 0.001286 0.006275 0.008576 0.001424 0.001916 0.006129 0.008556 0.001605 0.001617
Nuclear Periphery 0.000045 0.000083 0.000070 0.000147 0.000075 0.000091 0.000947 0.000103 0.000059 0.000090 0.000153 0.000124
Nuclear Periphery Foci 0.002502 0.004688 0.001802 0.003532 0.002611 0.002330 0.017313 0.003119 0.002553 0.002494 0.003284 0.003315
Nucleolus 0.000067 0.000057 0.000082 0.000161 0.000058 0.000060 0.000243 0.000144 0.000063 0.000060 0.000098 0.000152
Nucleus 0.070441 0.089269 0.033861 0.022794 0.115968 0.094985 0.075145 0.038046 0.091803 0.094587 0.037804 0.030812
Peroxisomes 0.000013 0.000044 0.000044 0.000251 0.000011 0.000041 0.000172 0.000217 0.000012 0.000041 0.000056 0.000233
Vacuole 0.004243 0.011002 0.018033 0.054960 0.004726 0.004932 0.179763 0.042439 0.004469 0.005355 0.033481 0.048378
Vacuole Periphery 0.000961 0.000208 0.000505 0.001458 0.000911 0.000860 0.001884 0.001484 0.000938 0.000815 0.000636 0.001472

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.96 -0.62 -1.56 -0.75 -1.52 -0.02 -9.41 -1.98 -1.91 2.32 -1.18 -2.59 -2.19 -1.93 -1.91
Bud Neck -4.43 -9.25 -14.87 -3.68 -9.51 -0.10 -12.57 -9.79 -9.76 10.68 -2.36 -12.21 -15.80 -13.59 -0.56
Bud Site -4.57 -5.52 -11.93 -2.64 -8.66 -0.73 -8.07 -8.94 -7.78 4.30 -2.18 -7.75 -14.60 -10.16 -7.79
Cell Periphery -4.57 -2.54 -10.61 -3.91 -10.02 2.43 -11.17 -5.74 -6.68 4.37 0.32 -5.74 -11.24 -11.32 -9.15
Cytoplasm 6.38 -0.68 14.15 -0.43 14.46 -2.99 18.95 7.65 10.38 -15.53 4.25 2.18 15.57 10.71 11.53
Cytoplasmic Foci -4.49 -2.05 -5.08 3.90 -3.50 1.80 -11.92 -6.28 -8.60 10.36 -2.10 -7.06 -8.24 -4.09 -0.06
Eisosomes -2.90 -2.45 -8.10 -2.08 -7.37 0.14 -14.58 -7.67 -6.10 10.06 -0.91 -8.36 -11.25 -7.21 -6.44
Endoplasmic Reticulum 2.53 4.43 3.09 0.43 -2.41 0.85 -0.61 3.40 1.19 8.20 0.28 4.05 4.25 1.18 1.06
Endosome -1.94 -3.93 -4.46 -0.89 -1.44 -0.86 -8.91 -2.57 -2.05 6.60 -1.51 -7.39 -3.78 -2.20 0.24
Golgi -1.97 -3.62 -2.51 -0.49 -1.60 0.51 -7.63 -1.93 -2.17 2.71 -0.63 -4.37 -3.19 -2.90 -1.54
Lipid Particles -2.54 -4.94 -11.38 -5.00 -9.13 2.07 -11.15 -10.03 -10.33 7.03 0.66 -10.27 -15.05 -15.30 -7.33
Mitochondria 2.04 1.59 -0.35 -1.67 -1.34 -0.02 0.90 -0.41 -0.26 -0.88 -0.45 3.23 -0.62 0.14 -2.06
Mitotic Spindle -1.77 -3.40 -2.75 -2.26 -1.64 -1.91 -3.30 -3.79 -2.10 2.03 -2.62 -3.84 -4.76 -1.77 -2.10
None -1.24 10.69 11.64 5.27 3.76 -2.88 11.50 9.85 9.86 -2.97 -3.39 15.64 14.98 11.38 -0.44
Nuclear Periphery -4.15 -8.85 -4.20 -2.05 -2.21 -0.78 -5.10 -7.46 -4.32 4.65 -1.98 -6.41 -7.31 -3.84 1.34
Nuclear Periphery Foci -2.94 1.51 -2.95 1.42 -4.30 0.97 -9.45 -3.01 -3.66 8.82 0.10 -3.56 -4.11 -3.63 -0.78
Nucleolus 0.19 -1.81 -4.29 -6.03 -4.20 -0.65 -9.70 -4.41 -4.25 3.53 -0.03 -5.55 -5.93 -6.50 -3.27
Nucleus -2.76 11.68 12.17 5.82 2.24 4.62 4.06 14.14 11.83 4.96 -2.02 19.17 17.58 18.01 -0.71
Peroxisomes -3.27 -4.49 -3.43 -2.76 -2.79 -1.59 -10.37 -4.10 -2.87 1.80 -1.69 -7.14 -5.11 -3.76 -3.42
Vacuole -3.21 -15.82 -23.96 -14.22 -16.89 -1.70 -15.74 -21.98 -21.45 9.30 -3.16 -19.06 -32.35 -30.68 -13.38
Vacuole Periphery 1.38 0.91 -0.00 -2.54 -1.72 0.21 -1.06 0.16 -0.09 1.60 0.37 1.15 0.10 -0.36 -1.85