Standard name | |
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Human Ortholog | |
Description | Mitochondrial NADH-cytochrome b5 reductase; involved in ergosterol biosynthesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 7 | 6 | 11 | 8 | 1 | 0 | 2 | 2 | 1 | 2 | 4 | 3 | 3 | 3 | 0 | 0 | – | 10 | 21 | 9 | 4 | 3 | 6 |
Bud | 8 | 10 | 4 | 9 | 11 | 3 | 20 | 14 | 11 | 24 | 25 | 21 | 2 | 2 | 1 | 0 | 0 | – | 4 | 5 | 1 | 2 | 3 | 5 |
Bud Neck | 3 | 1 | 6 | 1 | 5 | 2 | 4 | 8 | 3 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 3 | 10 | 2 | 1 | 4 | 6 |
Bud Site | 2 | 1 | 0 | 0 | 1 | 3 | 4 | 1 | 10 | 4 | 2 | 6 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 8 | 11 | 7 | 4 | 0 | 0 | 0 | 2 | 1 | 5 | 2 | 6 | 1 | 6 | 5 | 0 | 0 | – | 1 | 4 | 1 | 0 | 0 | 0 |
Cytoplasm | 109 | 211 | 121 | 65 | 36 | 52 | 48 | 35 | 26 | 34 | 35 | 25 | 87 | 127 | 178 | 22 | 19 | – | 25 | 63 | 25 | 35 | 33 | 46 |
Endoplasmic Reticulum | 7 | 12 | 6 | 6 | 6 | 6 | 3 | 2 | 8 | 4 | 1 | 3 | 10 | 7 | 4 | 2 | 0 | – | 3 | 7 | 3 | 2 | 0 | 2 |
Endosome | 24 | 13 | 5 | 5 | 2 | 32 | 2 | 18 | 3 | 11 | 5 | 9 | 5 | 14 | 27 | 1 | 5 | – | 44 | 51 | 20 | 10 | 8 | 8 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 2 | 1 | 2 | 0 | 0 | – | 5 | 9 | 1 | 2 | 4 | 2 |
Mitochondria | 118 | 151 | 6 | 60 | 76 | 199 | 202 | 170 | 317 | 345 | 400 | 485 | 8 | 10 | 4 | 4 | 5 | – | 286 | 362 | 189 | 95 | 96 | 126 |
Nucleus | 2 | 1 | 1 | 1 | 2 | 11 | 18 | 15 | 11 | 10 | 14 | 10 | 0 | 1 | 0 | 2 | 1 | – | 0 | 1 | 1 | 0 | 2 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 11 | 4 | 10 | 0 | 0 | 1 | 0 | 0 | – | 1 | 1 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 2 | 4 | 6 | 6 | 7 | 0 | 0 | 0 | 0 | 0 | – | 2 | 2 | 0 | 1 | 1 | 0 |
Peroxisomes | 2 | 1 | 5 | 3 | 0 | 3 | 0 | 2 | 0 | 0 | 3 | 2 | 0 | 0 | 8 | 0 | 1 | – | 2 | 4 | 2 | 2 | 0 | 1 |
SpindlePole | 19 | 12 | 14 | 10 | 10 | 26 | 23 | 45 | 15 | 40 | 24 | 47 | 3 | 9 | 5 | 4 | 3 | – | 16 | 12 | 7 | 6 | 10 | 5 |
Vac/Vac Membrane | 40 | 33 | 8 | 1 | 3 | 34 | 6 | 25 | 9 | 19 | 27 | 22 | 9 | 6 | 10 | 0 | 11 | – | 24 | 44 | 4 | 11 | 10 | 16 |
Unique Cell Count | 293 | 418 | 166 | 152 | 134 | 316 | 267 | 289 | 348 | 418 | 455 | 552 | 126 | 173 | 228 | 28 | 36 | 443 | 612 | 280 | 183 | 190 | 236 | |
Labelled Cell Count | 347 | 466 | 189 | 177 | 160 | 374 | 335 | 342 | 424 | 516 | 550 | 662 | 130 | 186 | 248 | 36 | 45 | 443 | 612 | 280 | 183 | 190 | 236 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 4.1 | 5.2 | 4.9 | 4.5 | 4.8 | 3.8 | 5.2 | 4.0 | 4.7 | 4.3 | 4.6 | 5.5 | 5.7 | 5.3 | 5.0 | 5.9 | – | 6.4 | 6.1 | 6.8 |
Std Deviation (1e-4) | 0.6 | 0.8 | 1.0 | 1.2 | 1.2 | 1.3 | 1.3 | 1.5 | 0.9 | 1.1 | 1.1 | 1.1 | 0.9 | 1.2 | 1.3 | 1.8 | 1.8 | – | 5.2 | 3.4 | 4.0 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.2 | -0.11 | -0.46 | -0.0 | -0.37 | -0.16 | -0.26 | -0.18 | 0.08 | 0.12 | 0.03 | -0.05 | 0.17 | – | 0.29 | 0.24 | 0.39 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.5558 | 3.4302 | 3.4554 | 3.341 | 3.8762 | 3.6996 | 7.8105 | 7.8319 | 7.2404 | 7.016 | 6.9462 | 7.6527 | 3.3524 | 4.1519 | 3.3853 | 4.03 | 3.6545 | 3.6664 |
Actin | 0.0156 | 0.0185 | 0.0156 | 0.0029 | 0.006 | 0.0051 | 0.0209 | 0.0247 | 0.0277 | 0.0047 | 0.0513 | 0.012 | 0.0208 | 0.0187 | 0.0053 | 0.0058 | 0.0027 | 0.0034 |
Bud | 0.002 | 0.0029 | 0.0044 | 0.0054 | 0.0255 | 0.0016 | 0.0018 | 0.0052 | 0.0031 | 0.0067 | 0.0012 | 0.0006 | 0.0034 | 0.0043 | 0.002 | 0.0018 | 0.0003 | 0.001 |
Bud Neck | 0.0012 | 0.0009 | 0.0012 | 0.0039 | 0.0438 | 0.002 | 0.0024 | 0.0006 | 0.0013 | 0.0004 | 0.0007 | 0.0012 | 0.0045 | 0.001 | 0.0009 | 0.001 | 0.0002 | 0.0026 |
Bud Periphery | 0.0049 | 0.0035 | 0.0097 | 0.0044 | 0.008 | 0.0025 | 0.003 | 0.0071 | 0.0057 | 0.01 | 0.0019 | 0.0018 | 0.0068 | 0.0058 | 0.0044 | 0.0068 | 0.0011 | 0.0021 |
Bud Site | 0.0091 | 0.0366 | 0.0072 | 0.0073 | 0.0121 | 0.001 | 0.0194 | 0.018 | 0.0109 | 0.0016 | 0.0027 | 0.0012 | 0.0038 | 0.0377 | 0.01 | 0.0036 | 0.0003 | 0.0018 |
Cell Periphery | 0.003 | 0.0008 | 0.0008 | 0.0009 | 0.0013 | 0.0011 | 0.0011 | 0.0008 | 0.0018 | 0.0006 | 0.0003 | 0.0005 | 0.0007 | 0.0012 | 0.001 | 0.0011 | 0.0001 | 0.0008 |
Cytoplasm | 0.0072 | 0.0185 | 0.0068 | 0.0129 | 0.0168 | 0.0105 | 0.0044 | 0.0125 | 0.0177 | 0.0038 | 0.0025 | 0.01 | 0.0032 | 0.0092 | 0.0034 | 0.0366 | 0.0054 | 0.0105 |
Cytoplasmic Foci | 0.0385 | 0.0519 | 0.0292 | 0.019 | 0.0337 | 0.0222 | 0.0295 | 0.0332 | 0.0324 | 0.0064 | 0.0248 | 0.022 | 0.0321 | 0.0681 | 0.0308 | 0.0142 | 0.0179 | 0.0357 |
Eisosomes | 0.0013 | 0.0003 | 0.0008 | 0.0002 | 0.0003 | 0.0004 | 0.0005 | 0.0006 | 0.0007 | 0.0001 | 0.0002 | 0.0004 | 0.0005 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0003 |
Endoplasmic Reticulum | 0.0067 | 0.0018 | 0.0023 | 0.0005 | 0.0004 | 0.0013 | 0.0084 | 0.0014 | 0.0009 | 0.0013 | 0.0008 | 0.0006 | 0.0009 | 0.0008 | 0.0006 | 0.0029 | 0.0002 | 0.0007 |
Endosome | 0.0652 | 0.1068 | 0.0659 | 0.0726 | 0.0339 | 0.0521 | 0.0796 | 0.0767 | 0.0663 | 0.0226 | 0.1148 | 0.0508 | 0.0735 | 0.0861 | 0.0695 | 0.0402 | 0.0567 | 0.0517 |
Golgi | 0.045 | 0.074 | 0.0511 | 0.0241 | 0.0433 | 0.0556 | 0.0886 | 0.0729 | 0.0611 | 0.0073 | 0.0942 | 0.0673 | 0.0508 | 0.0813 | 0.0587 | 0.0237 | 0.0647 | 0.0485 |
Lipid Particles | 0.0319 | 0.0146 | 0.016 | 0.0016 | 0.0144 | 0.0132 | 0.0083 | 0.0047 | 0.0086 | 0.0015 | 0.003 | 0.0055 | 0.0353 | 0.0035 | 0.0096 | 0.0015 | 0.0011 | 0.0113 |
Mitochondria | 0.6825 | 0.6148 | 0.7069 | 0.8226 | 0.7175 | 0.7875 | 0.6829 | 0.6827 | 0.7044 | 0.8979 | 0.6807 | 0.797 | 0.6795 | 0.6139 | 0.7534 | 0.8342 | 0.8397 | 0.7776 |
None | 0.0157 | 0.0054 | 0.0149 | 0.0049 | 0.0051 | 0.0125 | 0.0007 | 0.0012 | 0.0005 | 0.0017 | 0.0003 | 0.0028 | 0.0223 | 0.0013 | 0.0021 | 0.0083 | 0.0009 | 0.0058 |
Nuclear Periphery | 0.0022 | 0.0053 | 0.002 | 0.0002 | 0.0002 | 0.0006 | 0.012 | 0.0003 | 0.0004 | 0.0095 | 0.0004 | 0.0004 | 0.0016 | 0.0005 | 0.0003 | 0.0004 | 0 | 0.0002 |
Nucleolus | 0.0011 | 0.0021 | 0.0006 | 0.0006 | 0.0008 | 0.0005 | 0.0003 | 0.0002 | 0.0014 | 0.0006 | 0.0001 | 0.0002 | 0.003 | 0.0006 | 0.0005 | 0.0002 | 0 | 0.0004 |
Nucleus | 0.0008 | 0.0021 | 0.0006 | 0.0005 | 0.0019 | 0.0008 | 0.0075 | 0.0004 | 0.001 | 0.0033 | 0.0002 | 0.0003 | 0.0023 | 0.0009 | 0.0003 | 0.0007 | 0.0001 | 0.0004 |
Peroxisomes | 0.0321 | 0.0179 | 0.0513 | 0.0024 | 0.0292 | 0.0174 | 0.0167 | 0.0487 | 0.0304 | 0.0066 | 0.007 | 0.0218 | 0.0335 | 0.0456 | 0.0357 | 0.0084 | 0.0058 | 0.0369 |
Punctate Nuclear | 0.0132 | 0.0009 | 0.0006 | 0.0004 | 0.0003 | 0.0005 | 0.0006 | 0.0002 | 0.0003 | 0.0025 | 0.0001 | 0.0002 | 0.0051 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0004 |
Vacuole | 0.0149 | 0.0138 | 0.0068 | 0.0093 | 0.0031 | 0.0055 | 0.0052 | 0.0053 | 0.0171 | 0.0057 | 0.0064 | 0.0017 | 0.0114 | 0.0099 | 0.0058 | 0.0043 | 0.001 | 0.0042 |
Vacuole Periphery | 0.006 | 0.0064 | 0.0053 | 0.0035 | 0.0026 | 0.0062 | 0.0063 | 0.0027 | 0.0062 | 0.0053 | 0.0066 | 0.0019 | 0.0047 | 0.009 | 0.0051 | 0.0037 | 0.0015 | 0.0039 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 49.7685 | 39.0644 | 36.2343 | 61.6707 | 33.8374 | 60.6597 | 55.4069 | 59.2411 | 63.1996 | 53.3706 |
Translational Efficiency | 1.0965 | 1.1715 | 1.49 | 1.0395 | 1.4411 | 1.2665 | 1.1695 | 1.2941 | 1.5656 | 1.2925 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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