Standard name | |
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Description | Mitochondrial NADH-cytochrome b5 reductase; involved in ergosterol biosynthesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 7 | 6 | 11 | 8 | 1 | 0 | 2 | 2 | 1 | 2 | 4 | 3 | 3 | 3 | 0 | 0 | – | 10 | 21 | 9 | 4 | 3 | 6 |
Bud | 8 | 10 | 4 | 9 | 11 | 3 | 20 | 14 | 11 | 24 | 25 | 21 | 2 | 2 | 1 | 0 | 0 | – | 4 | 5 | 1 | 2 | 3 | 5 |
Bud Neck | 3 | 1 | 6 | 1 | 5 | 2 | 4 | 8 | 3 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 3 | 10 | 2 | 1 | 4 | 6 |
Bud Site | 2 | 1 | 0 | 0 | 1 | 3 | 4 | 1 | 10 | 4 | 2 | 6 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 8 | 11 | 7 | 4 | 0 | 0 | 0 | 2 | 1 | 5 | 2 | 6 | 1 | 6 | 5 | 0 | 0 | – | 1 | 4 | 1 | 0 | 0 | 0 |
Cytoplasm | 109 | 211 | 121 | 65 | 36 | 52 | 48 | 35 | 26 | 34 | 35 | 25 | 87 | 127 | 178 | 22 | 19 | – | 25 | 63 | 25 | 35 | 33 | 46 |
Endoplasmic Reticulum | 7 | 12 | 6 | 6 | 6 | 6 | 3 | 2 | 8 | 4 | 1 | 3 | 10 | 7 | 4 | 2 | 0 | – | 3 | 7 | 3 | 2 | 0 | 2 |
Endosome | 24 | 13 | 5 | 5 | 2 | 32 | 2 | 18 | 3 | 11 | 5 | 9 | 5 | 14 | 27 | 1 | 5 | – | 44 | 51 | 20 | 10 | 8 | 8 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 2 | 1 | 2 | 0 | 0 | – | 5 | 9 | 1 | 2 | 4 | 2 |
Mitochondria | 118 | 151 | 6 | 60 | 76 | 199 | 202 | 170 | 317 | 345 | 400 | 485 | 8 | 10 | 4 | 4 | 5 | – | 286 | 362 | 189 | 95 | 96 | 126 |
Nucleus | 2 | 1 | 1 | 1 | 2 | 11 | 18 | 15 | 11 | 10 | 14 | 10 | 0 | 1 | 0 | 2 | 1 | – | 0 | 1 | 1 | 0 | 2 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 11 | 4 | 10 | 0 | 0 | 1 | 0 | 0 | – | 1 | 1 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 2 | 4 | 6 | 6 | 7 | 0 | 0 | 0 | 0 | 0 | – | 2 | 2 | 0 | 1 | 1 | 0 |
Peroxisomes | 2 | 1 | 5 | 3 | 0 | 3 | 0 | 2 | 0 | 0 | 3 | 2 | 0 | 0 | 8 | 0 | 1 | – | 2 | 4 | 2 | 2 | 0 | 1 |
SpindlePole | 19 | 12 | 14 | 10 | 10 | 26 | 23 | 45 | 15 | 40 | 24 | 47 | 3 | 9 | 5 | 4 | 3 | – | 16 | 12 | 7 | 6 | 10 | 5 |
Vac/Vac Membrane | 40 | 33 | 8 | 1 | 3 | 34 | 6 | 25 | 9 | 19 | 27 | 22 | 9 | 6 | 10 | 0 | 11 | – | 24 | 44 | 4 | 11 | 10 | 16 |
Unique Cell Count | 293 | 418 | 166 | 152 | 134 | 316 | 267 | 289 | 348 | 418 | 455 | 552 | 126 | 173 | 228 | 28 | 36 | 443 | 612 | 280 | 183 | 190 | 236 | |
Labelled Cell Count | 347 | 466 | 189 | 177 | 160 | 374 | 335 | 342 | 424 | 516 | 550 | 662 | 130 | 186 | 248 | 36 | 45 | 443 | 612 | 280 | 183 | 190 | 236 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 4.6 | 4.1 | 5.2 | 4.9 | 4.5 | 4.8 | 3.8 | 5.2 | 4.0 | 4.7 | 4.3 | 4.6 | 5.5 | 5.7 | 5.3 | 5.0 | 5.9 | – | 6.4 | 6.1 | 6.8 |
Std Deviation (1e-4) | 0.6 | 0.8 | 1.0 | 1.2 | 1.2 | 1.3 | 1.3 | 1.5 | 0.9 | 1.1 | 1.1 | 1.1 | 0.9 | 1.2 | 1.3 | 1.8 | 1.8 | – | 5.2 | 3.4 | 4.0 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.2 | -0.11 | -0.46 | -0.0 | -0.37 | -0.16 | -0.26 | -0.18 | 0.08 | 0.12 | 0.03 | -0.05 | 0.17 | – | 0.29 | 0.24 | 0.39 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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