Standard name | |
---|---|
Human Ortholog | |
Description | Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 2 | 2 | 5 | 2 | 1 | 8 | 2 | 3 | 2 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 3 | 16 | 17 | 0 | 1 | 1 |
Bud | 0 | 10 | 5 | 5 | 3 | 6 | 3 | 14 | 10 | 11 | 8 | 4 | 1 | 1 | 1 | 1 | 0 | 0 | 6 | 27 | 11 | 1 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 14 | 5 | 0 | 3 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 8 | 4 | 5 | 11 | 28 | 9 | 11 | 5 | 8 | 2 | 10 | 6 | 7 | 3 | 11 | 7 | 14 | 1 | 3 | 2 | 1 | 2 | 0 |
Cytoplasm | 111 | 235 | 153 | 93 | 101 | 205 | 244 | 341 | 195 | 276 | 140 | 150 | 123 | 205 | 234 | 120 | 50 | 44 | 51 | 114 | 57 | 54 | 106 | 80 |
Endoplasmic Reticulum | 10 | 50 | 23 | 10 | 49 | 31 | 23 | 19 | 6 | 8 | 3 | 5 | 12 | 7 | 3 | 5 | 2 | 1 | 44 | 100 | 76 | 21 | 29 | 11 |
Endosome | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 3 | 7 | 4 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 0 | 0 | 0 |
Mitochondria | 4 | 2 | 5 | 1 | 0 | 4 | 9 | 11 | 9 | 15 | 8 | 20 | 3 | 0 | 2 | 1 | 0 | 1 | 3 | 11 | 11 | 0 | 2 | 4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 5 | 2 | 2 | 4 | 20 | 14 | 8 | 13 | 9 | 12 | 0 | 0 | 1 | 7 | 5 | 3 | 10 | 19 | 9 | 2 | 7 | 10 |
Unique Cell Count | 121 | 283 | 178 | 111 | 150 | 251 | 287 | 386 | 215 | 301 | 157 | 173 | 137 | 212 | 238 | 135 | 60 | 56 | 133 | 321 | 208 | 83 | 159 | 119 |
Labelled Cell Count | 126 | 310 | 198 | 121 | 171 | 281 | 311 | 424 | 237 | 335 | 175 | 202 | 147 | 220 | 245 | 151 | 65 | 66 | 133 | 321 | 208 | 83 | 159 | 119 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 6.8 | 6.8 | 7.3 | 9.7 | 7.9 | 6.6 | 6.3 | 6.1 | 5.5 | 5.3 | 5.2 | 6.3 | 5.7 | 5.4 | 12.1 | 14.5 | 15.8 | 6.4 | 5.7 | 6.6 |
Std Deviation (1e-4) | 1.3 | 1.8 | 2.4 | 2.4 | 2.8 | 2.5 | 2.1 | 1.8 | 1.7 | 1.7 | 1.5 | 1.8 | 1.7 | 1.4 | 1.6 | 5.4 | 6.4 | 7.2 | 3.7 | 1.6 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.52 | 0.22 | -0.03 | -0.11 | -0.16 | -0.31 | -0.36 | -0.4 | -0.12 | -0.24 | -0.33 | 0.84 | 1.09 | 1.22 | -0.07 | -0.24 | -0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0.5 | 0.9 | 0.5 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 2.2 | 3.5 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 0.6 | 0 | 2.4 | 0 | 0 |
Cytoplasm | -0.5 | -4.0 | -1.2 | -0.3 | 0.8 | 1.5 | 2.0 | 0.9 | 0.2 | 1.0 | 3.9 | 4.9 | 0.8 | -0.5 | -1.3 |
Endoplasmic Reticulum | -1.0 | 4.3 | -0.2 | -1.7 | -3.4 | -3.8 | -4.4 | -3.8 | -3.5 | -1.2 | -3.6 | -4.9 | -2.8 | -2.1 | -2.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0.2 | 0 | 0.7 | 1.1 | 1.1 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 1.9 | 0.5 | 0.5 | 0.8 | 1.3 | 1.8 | 0 | 0 | 0 | 1.1 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.6779 | 6.9179 | 5.3516 | 4.9324 | 3.7468 | 5.8118 | 5.8935 | 9.1645 | 8.3014 | 7.0985 | 7.2198 | 8.2997 | 4.8152 | 4.7043 | 7.2907 | 5.6715 | 4.5277 | 8.2854 |
Actin | 0.0445 | 0.0008 | 0.0131 | 0.0005 | 0.0234 | 0.0016 | 0.0273 | 0.0019 | 0.0296 | 0.0018 | 0.0376 | 0.0072 | 0.0018 | 0.0014 | 0.0054 | 0.0003 | 0.0095 | 0.0005 |
Bud | 0.0006 | 0.0008 | 0.0006 | 0.0001 | 0.0006 | 0.0001 | 0.0011 | 0.002 | 0.0004 | 0.0008 | 0.0013 | 0.0012 | 0.0258 | 0.0093 | 0.0002 | 0.0001 | 0.0192 | 0 |
Bud Neck | 0.0027 | 0.0005 | 0.0009 | 0.003 | 0.004 | 0.0096 | 0.0012 | 0.0008 | 0.0011 | 0.0007 | 0.0009 | 0.0117 | 0.0004 | 0.0007 | 0.0006 | 0.0006 | 0.002 | 0.0052 |
Bud Periphery | 0.0035 | 0.0047 | 0.0008 | 0.0002 | 0.0008 | 0.0002 | 0.0024 | 0.0041 | 0.0008 | 0.0014 | 0.0109 | 0.0008 | 0.0106 | 0.0382 | 0.0001 | 0.0001 | 0.0112 | 0 |
Bud Site | 0.0018 | 0.0034 | 0.0033 | 0.0002 | 0.0042 | 0.0001 | 0.0047 | 0.0058 | 0.0066 | 0.0015 | 0.0007 | 0.0003 | 0.0086 | 0.012 | 0.0014 | 0.0001 | 0.0026 | 0.0001 |
Cell Periphery | 0.0102 | 0.0153 | 0.0034 | 0.0064 | 0.0014 | 0.001 | 0.004 | 0.0047 | 0.0036 | 0.0041 | 0.0061 | 0.0016 | 0.0039 | 0.0121 | 0.0009 | 0.0001 | 0.001 | 0.0006 |
Cytoplasm | 0.3312 | 0.4641 | 0.5305 | 0.6604 | 0.6982 | 0.4845 | 0.3903 | 0.4548 | 0.5075 | 0.5172 | 0.5583 | 0.4702 | 0.4662 | 0.5143 | 0.4975 | 0.7236 | 0.6636 | 0.515 |
Cytoplasmic Foci | 0.0238 | 0.0021 | 0.0133 | 0.0036 | 0.0275 | 0.0096 | 0.0408 | 0.0108 | 0.0106 | 0.0075 | 0.0095 | 0.0082 | 0.0153 | 0.0075 | 0.0275 | 0.0143 | 0.0211 | 0.0054 |
Eisosomes | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.3893 | 0.3595 | 0.2958 | 0.2866 | 0.0358 | 0.4164 | 0.2979 | 0.4309 | 0.3349 | 0.3613 | 0.3128 | 0.4324 | 0.2158 | 0.1386 | 0.308 | 0.1656 | 0.1047 | 0.377 |
Endosome | 0.0212 | 0.004 | 0.0315 | 0.0055 | 0.0678 | 0.0132 | 0.0618 | 0.026 | 0.0193 | 0.0331 | 0.0125 | 0.0168 | 0.0954 | 0.013 | 0.0367 | 0.0375 | 0.0486 | 0.029 |
Golgi | 0.0049 | 0.0005 | 0.0036 | 0.0003 | 0.0021 | 0.0011 | 0.0224 | 0.0032 | 0.0051 | 0.0046 | 0.0032 | 0.0033 | 0.0048 | 0.002 | 0.0093 | 0.0026 | 0.033 | 0.0032 |
Lipid Particles | 0.0167 | 0.0047 | 0.0105 | 0.0024 | 0.0081 | 0.0039 | 0.0287 | 0.0231 | 0.0125 | 0.0103 | 0.0064 | 0.0035 | 0.0085 | 0.004 | 0.0071 | 0.0016 | 0.0032 | 0.003 |
Mitochondria | 0.0012 | 0.0001 | 0.0091 | 0.0001 | 0.0005 | 0.0002 | 0.0084 | 0.0008 | 0.0027 | 0.0015 | 0.0011 | 0.0076 | 0.0161 | 0.0157 | 0.0006 | 0.0002 | 0.0289 | 0.0001 |
None | 0.0642 | 0.0636 | 0.0416 | 0.008 | 0.0391 | 0.0346 | 0.0679 | 0.0043 | 0.0388 | 0.0036 | 0.0107 | 0.0136 | 0.0785 | 0.1728 | 0.0584 | 0.0427 | 0.0114 | 0.0396 |
Nuclear Periphery | 0.0121 | 0.0132 | 0.0165 | 0.0064 | 0.0253 | 0.0046 | 0.0096 | 0.0066 | 0.0057 | 0.0064 | 0.0041 | 0.0046 | 0.009 | 0.0107 | 0.0111 | 0.0031 | 0.0042 | 0.0056 |
Nucleolus | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0007 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0074 | 0.0005 | 0.0001 | 0 | 0.0002 | 0 |
Nucleus | 0.0027 | 0.0022 | 0.0022 | 0.0022 | 0.0201 | 0.0015 | 0.0024 | 0.0019 | 0.0018 | 0.002 | 0.0016 | 0.0019 | 0.0091 | 0.0054 | 0.0016 | 0.0009 | 0.0031 | 0.0019 |
Peroxisomes | 0.0017 | 0 | 0.0018 | 0 | 0.0003 | 0.0002 | 0.012 | 0.0001 | 0.0054 | 0.0001 | 0.0058 | 0.0006 | 0.001 | 0.0002 | 0.0072 | 0 | 0.0018 | 0 |
Punctate Nuclear | 0.0017 | 0.0004 | 0.001 | 0.0003 | 0.0088 | 0.0004 | 0.0017 | 0.0006 | 0.0008 | 0.0007 | 0.0039 | 0.0006 | 0.0014 | 0.001 | 0.0009 | 0.0002 | 0.0008 | 0.0002 |
Vacuole | 0.063 | 0.0589 | 0.0181 | 0.0135 | 0.0291 | 0.0166 | 0.0134 | 0.0169 | 0.012 | 0.039 | 0.0117 | 0.0133 | 0.0169 | 0.0374 | 0.0245 | 0.0055 | 0.0263 | 0.013 |
Vacuole Periphery | 0.0022 | 0.0009 | 0.002 | 0.0003 | 0.0018 | 0.0006 | 0.0016 | 0.0006 | 0.0006 | 0.0023 | 0.0006 | 0.0005 | 0.0035 | 0.0028 | 0.0008 | 0.0007 | 0.0033 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 582.111 | 209.0548 | 81.1659 | 192.213 | 390.0389 | 429.1054 | 127.6103 | 72.2153 | 150.8985 | 453.5565 |
Translational Efficiency | 1.3991 | 1.2262 | 1.1044 | 1.0861 | 1.3568 | 2.0305 | 1.4299 | 1.2085 | 1.4758 | 1.492 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1765 | 561 | 130 | 1368 | 1365 | 447 | 235 | 261 | 3130 | 1008 | 365 | 1629 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 774.36 | 870.06 | 1379.73 | 1337.31 | 748.42 | 792.11 | 1292.20 | 2020.42 | 763.05 | 835.49 | 1323.38 | 1446.76 |
Standard Deviation | 104.08 | 207.76 | 223.96 | 285.82 | 95.97 | 133.61 | 163.68 | 506.24 | 101.44 | 182.87 | 192.02 | 415.27 |
Intensity Change Log 2 | — | 0.168110 | 0.833310 | 0.788258 | — | 0.081853 | 0.787909 | 1.432735 | — | 0.126360 | 0.811175 | 1.140779 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000706 | 0.002715 | 0.002585 | 0.006423 | 0.000231 | 0.005242 | 0.001967 | 0.002220 | 0.000499 | 0.003836 | 0.002187 | 0.005750 |
Bud Neck | 0.029279 | 0.028415 | 0.005505 | 0.011415 | 0.010050 | 0.032473 | 0.005613 | 0.032171 | 0.020894 | 0.030215 | 0.005575 | 0.014740 |
Bud Site | 0.017696 | 0.042795 | 0.020390 | 0.065018 | 0.006349 | 0.037105 | 0.021799 | 0.048569 | 0.012748 | 0.040272 | 0.021297 | 0.062383 |
Cell Periphery | 0.001497 | 0.001421 | 0.004898 | 0.011246 | 0.000379 | 0.001306 | 0.002132 | 0.001668 | 0.001010 | 0.001370 | 0.003117 | 0.009712 |
Cytoplasm | 0.460921* | 0.309006* | 0.161782* | 0.442489* | 0.504833* | 0.284790* | 0.251055* | 0.562061* | 0.480071* | 0.298267* | 0.219259* | 0.461647* |
Cytoplasmic Foci | 0.147270 | 0.274232* | 0.030169 | 0.041109 | 0.202409* | 0.264969* | 0.046879 | 0.031795 | 0.171316 | 0.270124* | 0.040928 | 0.039617 |
Eisosomes | 0.001312 | 0.000827 | 0.000179 | 0.000279 | 0.000378 | 0.000945 | 0.000153 | 0.000268 | 0.000905 | 0.000879 | 0.000162 | 0.000277 |
Endoplasmic Reticulum | 0.059679 | 0.058319 | 0.402878* | 0.092163 | 0.016568 | 0.055862 | 0.121666* | 0.015071 | 0.040878 | 0.057229 | 0.221824* | 0.079811 |
Endosome | 0.021473 | 0.054591 | 0.025444 | 0.010331 | 0.021091 | 0.070860 | 0.017226 | 0.008787 | 0.021307 | 0.061805 | 0.020153 | 0.010083 |
Golgi | 0.010261 | 0.016514 | 0.001848 | 0.005524 | 0.003340 | 0.015935 | 0.000764 | 0.002951 | 0.007243 | 0.016258 | 0.001150 | 0.005112 |
Lipid Particles | 0.030049 | 0.023081 | 0.036384 | 0.110588* | 0.018624 | 0.031467 | 0.038829 | 0.019123 | 0.025066 | 0.026800 | 0.037958 | 0.095934 |
Mitochondria | 0.004441 | 0.005025 | 0.000132 | 0.000367 | 0.002637 | 0.004605 | 0.000093 | 0.006350 | 0.003654 | 0.004839 | 0.000107 | 0.001326 |
Mitotic Spindle | 0.000150 | 0.005220 | 0.002313 | 0.003839 | 0.001204 | 0.001560 | 0.001863 | 0.001246 | 0.000609 | 0.003597 | 0.002023 | 0.003423 |
None | 0.006114 | 0.000439 | 0.000133 | 0.001597 | 0.003110 | 0.000455 | 0.000123 | 0.001245 | 0.004804 | 0.000446 | 0.000127 | 0.001541 |
Nuclear Periphery | 0.000495 | 0.000352 | 0.004898 | 0.000376 | 0.000956 | 0.000658 | 0.002864 | 0.000474 | 0.000696 | 0.000488 | 0.003589 | 0.000391 |
Nuclear Periphery Foci | 0.001906 | 0.002176 | 0.103400 | 0.028082 | 0.002557 | 0.002748 | 0.089025 | 0.002448 | 0.002190 | 0.002429 | 0.094145 | 0.023975 |
Nucleolus | 0.000995 | 0.000473 | 0.000177 | 0.000141 | 0.001106 | 0.000755 | 0.000084 | 0.000849 | 0.001044 | 0.000598 | 0.000117 | 0.000254 |
Nucleus | 0.075862 | 0.024542 | 0.028506 | 0.024547 | 0.094095 | 0.025400 | 0.028312 | 0.048049 | 0.083814 | 0.024922 | 0.028381 | 0.028312 |
Peroxisomes | 0.003408 | 0.010244 | 0.000423 | 0.001286 | 0.002842 | 0.009599 | 0.000371 | 0.002854 | 0.003161 | 0.009958 | 0.000389 | 0.001537 |
Vacuole | 0.124372 | 0.135453 | 0.167421 | 0.141784 | 0.105699 | 0.148732 | 0.368935* | 0.209676* | 0.116229 | 0.141341 | 0.297163* | 0.152662 |
Vacuole Periphery | 0.002113 | 0.004162 | 0.000536 | 0.001397 | 0.001540 | 0.004533 | 0.000246 | 0.002123 | 0.001863 | 0.004327 | 0.000349 | 0.001513 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.38 | -3.08 | -9.64 | -4.85 | -4.07 | -2.12 | -4.40 | -3.00 | 1.39 | -0.15 | -3.09 | -4.92 | -10.32 | -1.43 | -5.89 |
Bud Neck | 0.32 | 11.22 | 9.41 | 6.29 | -4.63 | -7.26 | 4.45 | -4.63 | 0.74 | -5.77 | -4.10 | 12.39 | 4.93 | 7.44 | -8.37 |
Bud Site | -6.19 | -0.50 | -13.22 | -4.22 | -6.66 | -6.45 | -3.17 | -7.17 | -0.68 | -3.39 | -8.89 | -2.14 | -16.13 | -4.90 | -9.15 |
Cell Periphery | 0.30 | -1.91 | -9.66 | -9.32 | -2.04 | -3.81 | -2.63 | -3.85 | -0.17 | 0.36 | -1.84 | -2.45 | -10.31 | -9.48 | -5.22 |
Cytoplasm | 12.67 | 14.54 | 2.05 | -9.99 | -13.07 | 16.68 | 13.90 | -2.99 | -13.81 | -12.92 | 20.45 | 18.85 | 2.38 | -15.28 | -15.91 |
Cytoplasmic Foci | -13.04 | 20.80 | 26.47 | 24.83 | -2.59 | -6.14 | 26.44 | 28.41 | 23.80 | 2.40 | -13.99 | 32.47 | 39.11 | 34.28 | -0.27 |
Eisosomes | 3.43 | 11.01 | 10.33 | 5.31 | -1.43 | -2.40 | 5.74 | 2.33 | 3.04 | -1.51 | 0.23 | 12.49 | 10.18 | 5.23 | -2.88 |
Endoplasmic Reticulum | 0.21 | -10.04 | -2.24 | -1.78 | 9.55 | -4.87 | -4.88 | 0.89 | 4.87 | 4.99 | -2.78 | -9.08 | -4.74 | -1.01 | 7.45 |
Endosome | -9.41 | -1.73 | 11.94 | 13.06 | 7.34 | -10.01 | 4.08 | 7.76 | 12.58 | 4.37 | -13.69 | 2.15 | 14.28 | 18.03 | 8.41 |
Golgi | -3.17 | 12.60 | 6.87 | 6.12 | -3.96 | -6.28 | 6.40 | 1.08 | 6.42 | -2.31 | -6.38 | 15.37 | 4.78 | 8.33 | -5.89 |
Lipid Particles | 3.02 | -0.76 | -14.09 | -15.01 | -7.09 | -4.59 | -2.96 | 0.19 | 2.86 | 2.26 | -0.90 | -1.92 | -14.61 | -13.66 | -9.90 |
Mitochondria | -0.38 | 10.16 | 9.70 | 3.32 | -3.35 | -1.55 | 4.04 | -0.95 | -0.18 | -1.99 | -1.17 | 9.73 | 4.74 | 3.71 | -2.42 |
Mitotic Spindle | -1.96 | -1.20 | -6.53 | 0.34 | -0.89 | -0.41 | -1.25 | -0.49 | -0.05 | 0.90 | -2.00 | -2.01 | -5.62 | -0.18 | -1.12 |
None | 8.41 | 8.90 | 6.32 | -2.82 | -3.78 | 4.35 | 4.90 | 3.17 | -2.49 | -4.06 | 9.36 | 10.09 | 6.43 | -3.14 | -4.24 |
Nuclear Periphery | 2.16 | -4.40 | 0.50 | -1.41 | 4.43 | 1.50 | -4.39 | 1.99 | 0.61 | 5.38 | 2.19 | -6.39 | 2.66 | 0.19 | 6.96 |
Nuclear Periphery Foci | -1.10 | -9.41 | -17.54 | -17.21 | 6.89 | -0.09 | -11.87 | 0.09 | 0.17 | 11.85 | -0.58 | -15.01 | -16.79 | -16.66 | 10.76 |
Nucleolus | 5.02 | 9.65 | 17.56 | 3.74 | 0.61 | 1.18 | 4.46 | 1.37 | -0.28 | -10.12 | 2.81 | 8.44 | 7.42 | 2.75 | -4.01 |
Nucleus | 18.26 | 7.20 | 18.82 | -2.12 | 0.30 | 17.12 | 13.87 | 9.14 | -4.46 | -2.87 | 24.85 | 15.07 | 23.21 | -3.57 | -0.18 |
Peroxisomes | -7.41 | 17.67 | 11.43 | 9.90 | -4.74 | -6.83 | 9.54 | 0.60 | 6.76 | -5.31 | -10.07 | 18.95 | 9.44 | 12.79 | -7.38 |
Vacuole | -1.60 | -3.31 | -8.10 | -4.57 | 0.76 | -5.10 | -18.27 | -9.58 | -5.52 | 9.13 | -4.64 | -15.58 | -12.63 | -5.94 | 10.67 |
Vacuole Periphery | -3.32 | 9.87 | 3.09 | 4.53 | -2.00 | -3.89 | 4.14 | -0.40 | 3.16 | -3.03 | -5.09 | 9.47 | 2.09 | 5.89 | -3.49 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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