Standard name
Human Ortholog
Description Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0
Bud 0 0 0 0.05 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0.05 0.08 0.06 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0.07 0.11 0 0 0 0 0 0.06 0 0 0 0.08 0.12 0.25 0 0 0 0 0 0
Cytoplasm 0.92 0.83 0.86 0.84 0.67 0.82 0.85 0.88 0.91 0.92 0.89 0.87 0.9 0.97 0.98 0.89 0.83 0.79 0.39 0.36 0.28 0.66 0.67 0.67
Endoplasmic Reticulum 0.08 0.18 0.13 0.09 0.33 0.12 0.08 0.05 0 0 0 0 0.09 0 0 0 0 0 0.34 0.31 0.37 0.26 0.19 0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.05 0.05 0.12 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 0 0 0 0.06 0.07 0 0 0 0.05 0.08 0.05 0.08 0.06 0.05 0 0.05 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 2 2 5 2 1 8 2 3 2 1 1 0 0 2 0 0 3 16 17 0 1 1
Bud 0 10 5 5 3 6 3 14 10 11 8 4 1 1 1 1 0 0 6 27 11 1 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 14 5 0 3 2
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 8 4 5 11 28 9 11 5 8 2 10 6 7 3 11 7 14 1 3 2 1 2 0
Cytoplasm 111 235 153 93 101 205 244 341 195 276 140 150 123 205 234 120 50 44 51 114 57 54 106 80
Endoplasmic Reticulum 10 50 23 10 49 31 23 19 6 8 3 5 12 7 3 5 2 1 44 100 76 21 29 11
Endosome 0 1 1 2 0 0 1 5 0 1 0 0 0 0 0 3 0 2 3 7 4 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 3 0 0 0
Mitochondria 4 2 5 1 0 4 9 11 9 15 8 20 3 0 2 1 0 1 3 11 11 0 2 4
Nucleus 0 0 0 0 0 0 1 1 2 0 1 0 1 0 0 0 0 1 0 0 0 0 1 0
Nuclear Periphery 1 1 0 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 2 5 2 2 4 20 14 8 13 9 12 0 0 1 7 5 3 10 19 9 2 7 10
Unique Cell Count 121 283 178 111 150 251 287 386 215 301 157 173 137 212 238 135 60 56 133 321 208 83 159 119
Labelled Cell Count 126 310 198 121 171 281 311 424 237 335 175 202 147 220 245 151 65 66 133 321 208 83 159 119


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 6.8 6.8 7.3 9.7 7.9 6.6 6.3 6.1 5.5 5.3 5.2 6.3 5.7 5.4 12.1 14.5 15.8 6.4 5.7 6.6
Std Deviation (1e-4) 1.3 1.8 2.4 2.4 2.8 2.5 2.1 1.8 1.7 1.7 1.5 1.8 1.7 1.4 1.6 5.4 6.4 7.2 3.7 1.6 2.3
Intensity Change (Log2) – – – 0.11 0.52 0.22 -0.03 -0.11 -0.16 -0.31 -0.36 -0.4 -0.12 -0.24 -0.33 0.84 1.09 1.22 -0.07 -0.24 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.5 0.9 0.5 1.1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.2 3.5 0 0 0 0 0 1.7 0 0.6 0 2.4 0 0
Cytoplasm -0.5 -4.0 -1.2 -0.3 0.8 1.5 2.0 0.9 0.2 1.0 3.9 4.9 0.8 -0.5 -1.3
Endoplasmic Reticulum -1.0 4.3 -0.2 -1.7 -3.4 -3.8 -4.4 -3.8 -3.5 -1.2 -3.6 -4.9 -2.8 -2.1 -2.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.2 0 0.7 1.1 1.1 3.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 1.9 0.5 0.5 0.8 1.3 1.8 0 0 0 1.1 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6779 6.9179 5.3516 4.9324 3.7468 5.8118 5.8935 9.1645 8.3014 7.0985 7.2198 8.2997 4.8152 4.7043 7.2907 5.6715 4.5277 8.2854
Actin 0.0445 0.0008 0.0131 0.0005 0.0234 0.0016 0.0273 0.0019 0.0296 0.0018 0.0376 0.0072 0.0018 0.0014 0.0054 0.0003 0.0095 0.0005
Bud 0.0006 0.0008 0.0006 0.0001 0.0006 0.0001 0.0011 0.002 0.0004 0.0008 0.0013 0.0012 0.0258 0.0093 0.0002 0.0001 0.0192 0
Bud Neck 0.0027 0.0005 0.0009 0.003 0.004 0.0096 0.0012 0.0008 0.0011 0.0007 0.0009 0.0117 0.0004 0.0007 0.0006 0.0006 0.002 0.0052
Bud Periphery 0.0035 0.0047 0.0008 0.0002 0.0008 0.0002 0.0024 0.0041 0.0008 0.0014 0.0109 0.0008 0.0106 0.0382 0.0001 0.0001 0.0112 0
Bud Site 0.0018 0.0034 0.0033 0.0002 0.0042 0.0001 0.0047 0.0058 0.0066 0.0015 0.0007 0.0003 0.0086 0.012 0.0014 0.0001 0.0026 0.0001
Cell Periphery 0.0102 0.0153 0.0034 0.0064 0.0014 0.001 0.004 0.0047 0.0036 0.0041 0.0061 0.0016 0.0039 0.0121 0.0009 0.0001 0.001 0.0006
Cytoplasm 0.3312 0.4641 0.5305 0.6604 0.6982 0.4845 0.3903 0.4548 0.5075 0.5172 0.5583 0.4702 0.4662 0.5143 0.4975 0.7236 0.6636 0.515
Cytoplasmic Foci 0.0238 0.0021 0.0133 0.0036 0.0275 0.0096 0.0408 0.0108 0.0106 0.0075 0.0095 0.0082 0.0153 0.0075 0.0275 0.0143 0.0211 0.0054
Eisosomes 0.0006 0.0001 0.0002 0 0.0002 0 0.0005 0 0.0002 0.0001 0.0005 0.0001 0.0003 0.0003 0 0 0.0001 0
Endoplasmic Reticulum 0.3893 0.3595 0.2958 0.2866 0.0358 0.4164 0.2979 0.4309 0.3349 0.3613 0.3128 0.4324 0.2158 0.1386 0.308 0.1656 0.1047 0.377
Endosome 0.0212 0.004 0.0315 0.0055 0.0678 0.0132 0.0618 0.026 0.0193 0.0331 0.0125 0.0168 0.0954 0.013 0.0367 0.0375 0.0486 0.029
Golgi 0.0049 0.0005 0.0036 0.0003 0.0021 0.0011 0.0224 0.0032 0.0051 0.0046 0.0032 0.0033 0.0048 0.002 0.0093 0.0026 0.033 0.0032
Lipid Particles 0.0167 0.0047 0.0105 0.0024 0.0081 0.0039 0.0287 0.0231 0.0125 0.0103 0.0064 0.0035 0.0085 0.004 0.0071 0.0016 0.0032 0.003
Mitochondria 0.0012 0.0001 0.0091 0.0001 0.0005 0.0002 0.0084 0.0008 0.0027 0.0015 0.0011 0.0076 0.0161 0.0157 0.0006 0.0002 0.0289 0.0001
None 0.0642 0.0636 0.0416 0.008 0.0391 0.0346 0.0679 0.0043 0.0388 0.0036 0.0107 0.0136 0.0785 0.1728 0.0584 0.0427 0.0114 0.0396
Nuclear Periphery 0.0121 0.0132 0.0165 0.0064 0.0253 0.0046 0.0096 0.0066 0.0057 0.0064 0.0041 0.0046 0.009 0.0107 0.0111 0.0031 0.0042 0.0056
Nucleolus 0.0003 0.0002 0.0002 0 0.0007 0 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0074 0.0005 0.0001 0 0.0002 0
Nucleus 0.0027 0.0022 0.0022 0.0022 0.0201 0.0015 0.0024 0.0019 0.0018 0.002 0.0016 0.0019 0.0091 0.0054 0.0016 0.0009 0.0031 0.0019
Peroxisomes 0.0017 0 0.0018 0 0.0003 0.0002 0.012 0.0001 0.0054 0.0001 0.0058 0.0006 0.001 0.0002 0.0072 0 0.0018 0
Punctate Nuclear 0.0017 0.0004 0.001 0.0003 0.0088 0.0004 0.0017 0.0006 0.0008 0.0007 0.0039 0.0006 0.0014 0.001 0.0009 0.0002 0.0008 0.0002
Vacuole 0.063 0.0589 0.0181 0.0135 0.0291 0.0166 0.0134 0.0169 0.012 0.039 0.0117 0.0133 0.0169 0.0374 0.0245 0.0055 0.0263 0.013
Vacuole Periphery 0.0022 0.0009 0.002 0.0003 0.0018 0.0006 0.0016 0.0006 0.0006 0.0023 0.0006 0.0005 0.0035 0.0028 0.0008 0.0007 0.0033 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 582.111 209.0548 81.1659 192.213 390.0389 429.1054 127.6103 72.2153 150.8985 453.5565
Translational Efficiency 1.3991 1.2262 1.1044 1.0861 1.3568 2.0305 1.4299 1.2085 1.4758 1.492

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress
Localization
Cell Percentages ER (26%), cytoplasm (21%), mixed (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Crh1

Crh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Crh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available