Standard name
Description Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0
Bud 0 0 0 0.05 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0.05 0.08 0.06 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0.07 0.11 0 0 0 0 0 0.06 0 0 0 0.08 0.12 0.25 0 0 0 0 0 0
Cytoplasm 0.92 0.83 0.86 0.84 0.67 0.82 0.85 0.88 0.91 0.92 0.89 0.87 0.9 0.97 0.98 0.89 0.83 0.79 0.39 0.36 0.28 0.66 0.67 0.67
Endoplasmic Reticulum 0.08 0.18 0.13 0.09 0.33 0.12 0.08 0.05 0 0 0 0 0.09 0 0 0 0 0 0.34 0.31 0.37 0.26 0.19 0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.05 0.05 0.12 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 0 0 0 0.06 0.07 0 0 0 0.05 0.08 0.05 0.08 0.06 0.05 0 0.05 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 2 2 5 2 1 8 2 3 2 1 1 0 0 2 0 0 3 16 17 0 1 1
Bud 0 10 5 5 3 6 3 14 10 11 8 4 1 1 1 1 0 0 6 27 11 1 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 14 5 0 3 2
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 8 4 5 11 28 9 11 5 8 2 10 6 7 3 11 7 14 1 3 2 1 2 0
Cytoplasm 111 235 153 93 101 205 244 341 195 276 140 150 123 205 234 120 50 44 51 114 57 54 106 80
Endoplasmic Reticulum 10 50 23 10 49 31 23 19 6 8 3 5 12 7 3 5 2 1 44 100 76 21 29 11
Endosome 0 1 1 2 0 0 1 5 0 1 0 0 0 0 0 3 0 2 3 7 4 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 3 0 0 0
Mitochondria 4 2 5 1 0 4 9 11 9 15 8 20 3 0 2 1 0 1 3 11 11 0 2 4
Nucleus 0 0 0 0 0 0 1 1 2 0 1 0 1 0 0 0 0 1 0 0 0 0 1 0
Nuclear Periphery 1 1 0 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 2 5 2 2 4 20 14 8 13 9 12 0 0 1 7 5 3 10 19 9 2 7 10
Unique Cell Count 121 283 178 111 150 251 287 386 215 301 157 173 137 212 238 135 60 56 133 321 208 83 159 119
Labelled Cell Count 126 310 198 121 171 281 311 424 237 335 175 202 147 220 245 151 65 66 133 321 208 83 159 119


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 6.8 6.8 7.3 9.7 7.9 6.6 6.3 6.1 5.5 5.3 5.2 6.3 5.7 5.4 12.1 14.5 15.8 6.4 5.7 6.6
Std Deviation (1e-4) 1.3 1.8 2.4 2.4 2.8 2.5 2.1 1.8 1.7 1.7 1.5 1.8 1.7 1.4 1.6 5.4 6.4 7.2 3.7 1.6 2.3
Intensity Change (Log2) – – – 0.11 0.52 0.22 -0.03 -0.11 -0.16 -0.31 -0.36 -0.4 -0.12 -0.24 -0.33 0.84 1.09 1.22 -0.07 -0.24 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.5 0.9 0.5 1.1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.2 3.5 0 0 0 0 0 1.7 0 0.6 0 2.4 0 0
Cytoplasm -0.5 -4.0 -1.2 -0.3 0.8 1.5 2.0 0.9 0.2 1.0 3.9 4.9 0.8 -0.5 -1.3
Endoplasmic Reticulum -1.0 4.3 -0.2 -1.7 -3.4 -3.8 -4.4 -3.8 -3.5 -1.2 -3.6 -4.9 -2.8 -2.1 -2.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.2 0 0.7 1.1 1.1 3.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 1.9 0.5 0.5 0.8 1.3 1.8 0 0 0 1.1 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Crh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available