Standard name
Human Ortholog
Description Xylulokinase; converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 – – 1.0 0.97 0.94 – 0.76 0.75 0.72 0.61 – 1.0 0.67 0.94 0.97 0.94 0.94 0.96 0.96 0.84 0.89 0.85
Endoplasmic Reticulum 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – – 0 0 0 – 0.24 0.18 0.44 0.39 – 0.06 0.05 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – – 0 0 0 – 0.07 0 0 0.05 – 0 0.15 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – – 0 0 0 – 0 0 0 0 – 0 0.08 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – – 0 0 0 – 0 0.12 0.05 0.06 – 0 0 0.05 0 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 – – 0 0 0 – 0 1 2 1 – 0 0 0 0 0 0 1 0 0 0 0
Bud Neck 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 1 4 – – 1 0 2 – 0 4 1 0 – 0 0 9 13 6 0 0 0 0 0 0
Cytoplasm 219 204 – – 56 119 150 – 42 104 80 57 – 16 26 263 284 225 218 202 46 17 33 48
Endoplasmic Reticulum 2 3 – – 0 0 0 – 1 1 0 0 – 0 0 10 14 8 0 0 0 0 0 0
Endosome 0 0 – – 0 1 1 – 0 0 0 0 – 0 0 0 0 0 2 0 0 0 0 0
Golgi 0 1 – – 0 0 0 – 0 0 0 0 – 0 0 2 1 0 0 2 0 0 0 0
Mitochondria 4 0 – – 0 3 5 – 13 25 49 36 – 1 2 4 0 0 2 0 0 0 0 0
Nucleus 2 1 – – 0 1 5 – 4 4 1 5 – 0 6 10 8 5 0 2 0 0 0 0
Nuclear Periphery 2 0 – – 0 0 0 – 0 0 0 0 – 0 3 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 – – 0 0 0 – 0 0 0 1 – 0 1 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – 0 0 0 – 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 0 0 – – 0 1 3 – 1 16 5 6 – 0 1 15 7 20 1 0 0 0 0 1
Unique Cell Count 226 207 56 123 159 55 139 111 93 16 39 279 294 240 232 211 48 21 38 57
Labelled Cell Count 230 213 57 125 166 61 155 138 106 17 39 313 327 265 232 211 48 21 38 57


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 6.5 – – 7.7 7.0 7.3 – 5.7 6.3 6.0 5.6 – 6.8 6.0 14.2 14.7 13.9 – – 5.9
Std Deviation (1e-4) 0.8 1.1 – – 1.5 1.6 1.8 – 1.0 1.2 1.5 1.2 – 1.2 1.3 3.0 2.7 2.5 – – 1.1
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0998 1.4575 1.2131 1.371 1.0689 1.3369 1.0985 1.4139 1.2384 0.9393 0.9682 1.2657 1.0025 1.4374 1.116 1.4433 1.3735 1.1543
Actin 0.0236 0.0028 0.0038 0.0335 0.0438 0.0044 0.0382 0.0006 0.0182 0.0041 0.046 0.006 0.0291 0.0078 0.0066 0.0074 0.0004 0.003
Bud 0.0011 0.0112 0.0021 0.0098 0.0036 0.0007 0.0009 0.0014 0.0028 0.0071 0.0007 0.0002 0.0007 0.0005 0.0018 0.0006 0.0001 0.0012
Bud Neck 0.0115 0.0005 0.0005 0.0024 0.0122 0.0009 0.005 0.0002 0.0024 0.0008 0.0005 0.0009 0.0026 0.0002 0.0006 0.0005 0.0003 0.0018
Bud Periphery 0.002 0.0062 0.0014 0.0169 0.0058 0.0007 0.0011 0.0006 0.0033 0.0123 0.0009 0.0002 0.0008 0.0005 0.0014 0.0005 0 0.0012
Bud Site 0.0102 0.01 0.005 0.0171 0.0053 0.0008 0.0086 0.006 0.0295 0.002 0.0019 0.0003 0.008 0.0033 0.0062 0.0007 0.0003 0.0004
Cell Periphery 0.0003 0.0003 0.0001 0.0006 0.0002 0.0001 0.0005 0.0001 0.0003 0.0001 0.0001 0.0001 0.0005 0.0001 0.0007 0 0 0.0001
Cytoplasm 0.408 0.6072 0.4986 0.4934 0.459 0.6258 0.4087 0.6247 0.4835 0.5409 0.4302 0.6102 0.5797 0.8266 0.7251 0.8201 0.7602 0.8057
Cytoplasmic Foci 0.036 0.0119 0.0191 0.0134 0.0177 0.0228 0.0369 0.0105 0.0195 0.0133 0.0771 0.0161 0.0398 0.0066 0.0112 0.0453 0.0414 0.0143
Eisosomes 0.0003 0.0001 0.0001 0.0003 0.0003 0 0.0009 0.0001 0.0001 0 0.0002 0.0001 0.0006 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0024 0.0018 0.0023 0.0056 0.0037 0.0027 0.008 0.0011 0.0029 0.0012 0.0033 0.002 0.0074 0.0012 0.0037 0.0015 0.0026 0.0023
Endosome 0.0181 0.0056 0.0121 0.0048 0.0132 0.0068 0.0259 0.0014 0.0049 0.0015 0.0573 0.0103 0.0377 0.0011 0.0136 0.011 0.0104 0.0157
Golgi 0.0057 0.0004 0.003 0.0015 0.0105 0.0011 0.0082 0.0002 0.0025 0.0015 0.0133 0.0066 0.0119 0.002 0.0108 0.008 0.0015 0.0089
Lipid Particles 0.0079 0.0002 0.004 0.002 0.0015 0.0034 0.017 0.0002 0.0037 0.0003 0.0325 0.0048 0.0112 0.0001 0.0051 0.0021 0.0034 0.001
Mitochondria 0.0033 0.0011 0.0048 0.0013 0.0423 0.0051 0.0035 0.0002 0.0016 0.0024 0.0084 0.0097 0.013 0.0014 0.0076 0.0009 0.0003 0.0022
None 0.4382 0.3306 0.4233 0.3762 0.3504 0.3005 0.3831 0.3449 0.3904 0.3572 0.2519 0.3198 0.2073 0.1451 0.1816 0.0731 0.1436 0.1286
Nuclear Periphery 0.0054 0.0009 0.0032 0.0041 0.0098 0.0015 0.0151 0.0005 0.0013 0.0004 0.0147 0.0034 0.0049 0.0003 0.0015 0.0007 0.0012 0.0016
Nucleolus 0.001 0.0004 0.0005 0.0003 0.0005 0.0003 0.0008 0.0002 0.0007 0.0001 0.0007 0.0002 0.0006 0 0.0002 0.0001 0.0004 0.0002
Nucleus 0.0033 0.004 0.0036 0.0047 0.007 0.0068 0.0043 0.0029 0.0025 0.002 0.0028 0.0023 0.005 0.0011 0.0019 0.0025 0.0038 0.0038
Peroxisomes 0.0124 0.0013 0.002 0.0046 0.0041 0.0085 0.0191 0.0007 0.0254 0.0519 0.0214 0.0016 0.0273 0.0014 0.0139 0.0219 0.0085 0.0034
Punctate Nuclear 0.0064 0.0019 0.0067 0.005 0.0054 0.0062 0.0103 0.0027 0.0031 0.0006 0.029 0.0041 0.0059 0.0002 0.0007 0.0025 0.0209 0.0036
Vacuole 0.002 0.0014 0.0021 0.0021 0.002 0.0008 0.0023 0.0007 0.0012 0.0004 0.003 0.0007 0.0047 0.0005 0.0052 0.0007 0.0006 0.0008
Vacuole Periphery 0.0008 0.0002 0.0017 0.0005 0.0015 0.0002 0.0017 0.0001 0.0002 0.0001 0.0038 0.0005 0.0013 0 0.001 0.0002 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.8959 4.3766 2.4357 7.7798 9.5215 4.2815 10.3715 11.9468 13.1543 9.7608
Translational Efficiency 0.8671 0.9547 1.589 0.9212 0.5912 1.4125 0.8336 0.8722 0.9578 0.8576

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
143 1363 170 632 1397 2021 1758 1390 1540 3384 1928 2022

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 845.98 752.24 1434.70 976.85 742.17 753.28 1024.75 941.54 751.81 752.86 1060.90 952.58
Standard Deviation 175.29 121.98 177.12 148.45 92.15 100.98 136.29 139.25 107.07 109.92 182.25 143.13
Intensity Change Log 2 — -0.169431 0.762054 0.207513 — 0.021437 0.465450 0.343273 — -0.077085 0.630989 0.272549

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001164 0.000463 0.001228 0.000320 0.000131 0.000198 0.000208 0.001602 0.000227 0.000305 0.000298 0.001201
Bud Neck 0.021876 0.009739 0.003805 0.002558 0.003924 0.018433 0.001455 0.009978 0.005591 0.014931 0.001662 0.007659
Bud Site 0.045373 0.009784 0.006301 0.012807 0.002440 0.012656 0.005744 0.046503 0.006427 0.011500 0.005793 0.035971
Cell Periphery 0.000481 0.000079 0.000355 0.000141 0.000103 0.000090 0.000074 0.000152 0.000138 0.000085 0.000099 0.000149
Cytoplasm 0.518141* 0.619914* 0.391427* 0.553777* 0.662258* 0.565964* 0.647975* 0.557400* 0.648876* 0.587694* 0.625354* 0.556268*
Cytoplasmic Foci 0.162275 0.124143 0.013919 0.020323 0.139189 0.178963 0.007288 0.020163 0.141333 0.156883 0.007872 0.020213
Eisosomes 0.000112 0.000050 0.000038 0.000036 0.000061 0.000074 0.000015 0.000028 0.000066 0.000064 0.000017 0.000030
Endoplasmic Reticulum 0.004893 0.000795 0.004172 0.003631 0.000838 0.000666 0.001359 0.001444 0.001214 0.000718 0.001607 0.002128
Endosome 0.012129 0.012455 0.004301 0.006412 0.007031 0.017953 0.000900 0.011107 0.007504 0.015738 0.001200 0.009639
Golgi 0.001624 0.001540 0.000425 0.006382 0.001129 0.003995 0.000062 0.007750 0.001175 0.003006 0.000094 0.007322
Lipid Particles 0.004543 0.002781 0.002378 0.001897 0.003307 0.003635 0.000496 0.000940 0.003422 0.003291 0.000662 0.001239
Mitochondria 0.002515 0.002925 0.000445 0.000766 0.000716 0.004405 0.000251 0.007982 0.000883 0.003809 0.000268 0.005727
Mitotic Spindle 0.018643 0.004722 0.002058 0.022338 0.001165 0.011513 0.003311 0.085465 0.002788 0.008777 0.003201 0.065734
None 0.003723 0.004769 0.003345 0.005638 0.004069 0.002544 0.007869 0.008464 0.004037 0.003440 0.007470 0.007581
Nuclear Periphery 0.000709 0.000391 0.001609 0.001330 0.000425 0.000438 0.000807 0.002224 0.000451 0.000419 0.000877 0.001945
Nuclear Periphery Foci 0.006219 0.000840 0.009279 0.005093 0.001320 0.000559 0.001914 0.004733 0.001775 0.000673 0.002563 0.004846
Nucleolus 0.004279 0.001664 0.000218 0.000267 0.000777 0.001035 0.000123 0.000369 0.001102 0.001289 0.000131 0.000337
Nucleus 0.098311 0.115256 0.408948* 0.226940* 0.127150 0.084988 0.264965* 0.127138 0.124472 0.097180 0.277660* 0.158332*
Peroxisomes 0.007842 0.003226 0.000229 0.000283 0.001653 0.005889 0.000123 0.003754 0.002228 0.004817 0.000132 0.002669
Vacuole 0.083661 0.083022 0.145269 0.126851 0.041613 0.083796 0.054988 0.095980 0.045518 0.083484 0.062949 0.105629
Vacuole Periphery 0.001486 0.001439 0.000248 0.002211 0.000699 0.002204 0.000075 0.006824 0.000772 0.001896 0.000090 0.005382

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.90 -1.32 3.02 0.49 9.88 -1.28 -2.55 -4.63 -4.00 -3.68 -0.58 -2.65 -3.79 -1.97 -2.35
Bud Neck 1.99 2.67 3.17 7.72 5.30 -10.60 3.00 -1.92 8.60 -4.88 -8.28 3.99 0.47 10.09 -4.81
Bud Site 3.97 4.03 4.05 0.52 0.34 -7.87 -1.94 -7.46 -2.34 -6.01 -3.91 1.23 -4.98 -2.21 -5.92
Cell Periphery 3.50 0.00 3.12 -4.80 10.65 2.30 3.51 0.89 -0.49 -1.07 4.50 1.72 2.64 -2.21 1.59
Cytoplasm -4.17 5.54 1.50 12.10 -6.81 10.59 13.02 20.48 10.84 9.15 7.71 13.68 21.22 16.15 8.27
Cytoplasmic Foci 2.10 9.64 9.55 24.10 -0.83 -6.38 30.68 29.48 38.22 -4.30 -2.92 32.15 30.85 44.81 -5.97
Eisosomes 4.17 4.34 4.90 2.86 1.58 -2.83 14.26 9.71 12.23 -7.53 0.79 14.47 10.45 11.77 -8.10
Endoplasmic Reticulum 3.81 0.15 1.63 -8.22 3.10 0.93 -6.79 -4.37 -5.47 3.39 2.71 -5.32 -4.23 -9.66 1.39
Endosome -0.24 3.15 3.85 8.29 2.21 -8.95 10.85 5.19 12.47 -4.20 -8.79 11.25 6.47 14.85 -4.55
Golgi 0.03 2.38 -0.38 -0.41 -1.26 -5.39 4.63 -1.16 3.17 -3.42 -4.91 4.99 -1.24 1.93 -3.48
Lipid Particles 1.39 0.96 2.46 2.57 5.90 -0.68 8.34 7.20 7.91 -4.62 0.43 8.15 7.19 7.78 -3.05
Mitochondria -0.37 1.59 1.66 3.63 0.81 -4.91 4.01 -2.63 2.23 -3.40 -5.41 3.88 -1.96 3.12 -3.45
Mitotic Spindle 1.56 1.72 -0.01 -3.41 -3.86 -5.41 -3.60 -10.13 -6.81 -9.09 -3.94 -1.65 -10.05 -7.90 -9.61
None -0.85 0.72 -1.68 -1.05 -3.70 2.94 -3.68 -5.45 -8.20 -2.27 1.29 -3.51 -5.34 -6.79 -2.02
Nuclear Periphery 1.45 -6.26 -4.47 -13.31 3.23 -0.07 -6.11 -6.76 -7.26 -3.83 0.18 -7.02 -7.88 -9.80 -3.95
Nuclear Periphery Foci 2.75 -1.99 0.98 -9.35 5.69 2.33 -2.83 -1.23 -3.13 0.63 3.12 -3.32 -1.89 -5.77 0.95
Nucleolus 0.83 1.41 1.41 7.35 0.13 -1.29 4.68 2.33 3.87 -2.80 -0.82 3.45 2.62 7.30 -3.10
Nucleus -1.59 -13.94 -9.56 -12.57 8.16 9.71 -27.78 -12.33 -21.00 13.29 6.64 -30.56 -15.77 -23.63 14.80
Peroxisomes 2.59 4.37 4.41 8.98 2.57 -7.31 4.85 1.56 8.63 -2.73 -5.54 6.28 3.77 10.67 -2.79
Vacuole 1.47 -5.41 -7.63 -14.30 -1.11 -12.88 -15.92 -26.43 -19.91 -13.90 -12.42 -16.38 -30.47 -24.40 -15.22
Vacuole Periphery -0.01 2.70 1.19 1.69 -1.45 -3.22 3.77 -1.87 0.97 -3.41 -3.56 4.31 -1.59 1.27 -3.85
Standard name
Human Ortholog
Description Xylulokinase; converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains
Localization
Cell Percentages cytoplasm (73%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Xks1

Xks1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Xks1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available