Standard name
Description Xylulokinase; converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 1.00 0.97 0.94 0.76 0.75 0.72 0.61 1.00 0.67 0.94 0.97 0.94 0.94 0.96 0.96 0.84 0.89 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.24 0.18 0.44 0.39 0.06 0.05 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.07 0 0 0.05 0 0.15 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.12 0.05 0.06 0 0 0.05 0 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 0 1 2 1 0 0 0 0 0 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 4 1 0 2 0 4 1 0 0 0 9 13 6 0 0 0 0 0 0
Cytoplasm 219 204 56 119 150 42 104 80 57 16 26 263 284 225 218 202 46 17 33 48
Endoplasmic Reticulum 2 3 0 0 0 1 1 0 0 0 0 10 14 8 0 0 0 0 0 0
Endosome 0 0 0 1 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0
Golgi 0 1 0 0 0 0 0 0 0 0 0 2 1 0 0 2 0 0 0 0
Mitochondria 4 0 0 3 5 13 25 49 36 1 2 4 0 0 2 0 0 0 0 0
Nucleus 2 1 0 1 5 4 4 1 5 0 6 10 8 5 0 2 0 0 0 0
Nuclear Periphery 2 0 0 0 0 0 0 0 0 0 3 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 1 0 1 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 3 1 16 5 6 0 1 15 7 20 1 0 0 0 0 1
Unique Cell Count 226 207 56 123 159 55 139 111 93 16 39 279 294 240 232 211 48 21 38 57
Labelled Cell Count 230 213 57 125 166 61 155 138 106 17 39 313 327 265 232 211 48 21 38 57

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 6.5 7.7 7.0 7.3 5.7 6.3 6.0 5.6 6.8 6.0 14.2 14.7 13.9 5.9
Std Deviation (1e-4) 0.8 1.1 1.5 1.6 1.8 1.0 1.2 1.5 1.2 1.2 1.3 3.0 2.7 2.5 1.1
Intensity Change (Log2)

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole