Standard name
Human Ortholog
Description Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.13 0.07 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Nucleus 0.58 0.65 0.66 0.95 0.97 0.9 0.89 0.82 0.87 0.82 0.83 0.72 0.82 0.74 0.68 0.63 0.69 0.41 0.5 0.49 0.71 0.57 0.39
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.5 0.36 0.37 0.07 0 0.06 0.06 0.18 0.1 0.14 0.19 0.29 0.17 0.14 0.26 0.25 0.18 0.51 0.4 0.45 0.19 0.21 0.25
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.09 0.05 0 0 0.05 0.17 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 0 2 0 1 0 0 0 0 0 0 0 3 2 3 3 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 0 0 0 2 1 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 4 5 1 3 7 10 8 7 7 3 12 22 46 17 12 17 0 2 0 3 2 3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 4 9 0 0 0 0 0 1
Endosome 1 1 1 0 0 0 2 0 0 2 0 2 0 4 1 3 5 0 3 2 3 0 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 1 0
Mitochondria 1 15 1 0 0 0 3 18 17 8 10 0 0 0 11 10 6 0 1 0 0 0 5
Nucleus 120 138 149 186 250 207 244 213 265 211 257 177 257 260 161 132 188 92 116 135 193 193 134
Nuclear Periphery 0 0 2 1 6 1 2 1 1 0 5 0 0 1 3 1 1 0 0 1 1 2 5
Nucleolus 104 76 85 13 3 13 16 46 32 37 60 72 52 48 61 52 49 114 93 123 53 71 86
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 1 0 1 1 1 1 1 2 4 0 0 1 1 1 1 0 4 1
Vac/Vac Membrane 7 3 4 1 1 7 6 3 5 9 4 3 7 11 14 15 26 10 8 4 13 56 94
Unique Cell Count 206 211 227 196 257 230 273 260 305 257 311 245 313 352 236 211 274 226 233 275 274 339 346
Labelled Cell Count 236 237 247 204 264 236 283 293 330 277 340 268 340 377 270 229 304 226 233 275 274 339 346


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 9.5 12.7 12.6 11.1 9.8 9.2 8.2 8.3 8.9 8.7 13.6 12.5 11.9 10.9 14.2 14.1 13.7 13.5 13.4
Std Deviation (1e-4) 2.7 2.0 2.9 2.4 2.4 2.1 1.9 1.9 2.0 2.1 2.0 3.4 3.4 3.7 3.2 4.6 4.5 3.2 2.7 2.8
Intensity Change (Log2) -0.01 -0.2 -0.37 -0.47 -0.64 -0.61 -0.51 -0.55 0.1 -0.03 -0.1 -0.23 0.16 0.15 0.11 0.09 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.6 1.0 0.6 0.1 0.4 0 1.6 2.5 4.5 2.5 1.9 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.7 3.2 0 0 0 0 0 2.9 2.9 0
Nucleus 7.4 9.1 6.3 6.4 4.1 5.8 4.1 4.5 1.6 4.4 2.1 0.6 -0.7 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -7.5 -10.3 -8.3 -8.8 -4.9 -7.4 -5.8 -4.7 -1.9 -5.5 -6.6 -2.7 -2.9 -4.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0.9 0 0 0 1.2 0 0 0 1.0 2.3 2.7 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.9038 8.2944 7.829 6.9196 6.2197 7.9171 10.4508 11.6365 11.665 10.6424 11.0061 11.348 7.9769 9.7598 9.3119 8.556 7.5994 8.8657
Actin 0.0068 0.0033 0.0081 0.0026 0.0227 0.0006 0.0095 0.0004 0.0045 0 0 0.0028 0.0412 0.0036 0.0007 0.0735 0.013 0
Bud 0.0003 0.0001 0.0005 0.0001 0.0002 0.0001 0.0007 0.0002 0.0002 0.0001 0 0.0001 0.0006 0.0003 0 0.0004 0.0003 0.0001
Bud Neck 0.0006 0.0003 0.0017 0.0002 0.0007 0.0007 0.0008 0.0004 0.0004 0.0003 0.0003 0.0007 0.0012 0.0003 0.0002 0.0003 0.0006 0.0006
Bud Periphery 0.0005 0.0001 0.001 0.0001 0.0005 0.0002 0.0016 0.0003 0.0003 0.0001 0.0001 0.0002 0.0009 0.0004 0.0001 0.0004 0.0004 0.0001
Bud Site 0.0012 0.0009 0.0074 0.0001 0.0005 0.0001 0.0016 0.0005 0.0007 0.0002 0.0001 0.0002 0.0043 0.0055 0.0002 0.0007 0.0009 0.0001
Cell Periphery 0.0004 0.0002 0.0004 0 0.0002 0.0001 0.0005 0.0001 0.0002 0.0001 0 0.0001 0.0003 0.0003 0.0001 0.0001 0.0002 0.0001
Cytoplasm 0.0097 0.0053 0.0019 0.0003 0.0031 0.0003 0.01 0.0006 0.0058 0.0001 0 0.0004 0.0059 0.0001 0.0011 0.0002 0.0056 0
Cytoplasmic Foci 0.0183 0.0006 0.0027 0.0088 0.0039 0.0052 0.0225 0.0011 0.0067 0.0181 0 0.0019 0.0103 0.0001 0.0095 0.0009 0.0023 0
Eisosomes 0.0003 0 0.0001 0 0.0002 0 0.0004 0 0.0001 0 0 0 0.0005 0.0001 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0034 0.0006 0.0005 0.0001 0.0136 0 0.0073 0.0002 0.0052 0 0 0.0002 0.0028 0 0.0002 0.0003 0.0095 0
Endosome 0.0114 0.0005 0.0034 0.0026 0.0254 0.0012 0.0125 0.0024 0.0108 0.0003 0 0.0043 0.01 0.0002 0.0025 0.0013 0.0115 0
Golgi 0.0029 0.0012 0.0021 0.0021 0.0051 0.0004 0.003 0.0008 0.0042 0.0003 0 0.0037 0.0154 0.0002 0.0005 0.0024 0.0018 0
Lipid Particles 0.0055 0.0001 0.0021 0.0359 0.0094 0.0013 0.0043 0.0007 0.0018 0.0245 0 0.0023 0.0126 0.0002 0.0012 0.0005 0.0019 0.0001
Mitochondria 0.0084 0.0004 0.0134 0.0048 0.0056 0.0002 0.0073 0.0009 0.0078 0.0002 0.0002 0.0015 0.0283 0.0028 0.0002 0.0014 0.0022 0.0001
None 0.0066 0.0003 0.0019 0.0006 0.0017 0.0002 0.0168 0.0001 0.0056 0 0 0.0002 0.0015 0.0001 0.0021 0.0002 0.0053 0
Nuclear Periphery 0.0125 0.0008 0.0078 0.0013 0.0389 0.0009 0.0346 0.0028 0.0018 0.0004 0.0008 0.0022 0.0122 0.0008 0.0006 0.0005 0.0134 0.0002
Nucleolus 0.3299 0.2673 0.375 0.2752 0.2398 0.4227 0.3625 0.3787 0.4596 0.3754 0.2679 0.4661 0.2987 0.3366 0.5059 0.4252 0.3353 0.4396
Nucleus 0.5651 0.7164 0.5499 0.6509 0.6057 0.554 0.4617 0.6004 0.4712 0.5771 0.7295 0.5062 0.5309 0.6441 0.466 0.4901 0.5905 0.558
Peroxisomes 0.0026 0.0001 0.0046 0.0114 0.0026 0.0032 0.0086 0.0002 0.0006 0.0005 0 0.0006 0.0148 0.0005 0.0052 0.0008 0.0006 0
Punctate Nuclear 0.008 0.0006 0.0101 0.0021 0.0129 0.0081 0.0251 0.0071 0.009 0.002 0.0005 0.0056 0.0029 0.0003 0.0036 0.0005 0.0028 0.0008
Vacuole 0.0031 0.0006 0.003 0.0002 0.0041 0.0004 0.0046 0.0015 0.0028 0.0003 0.0003 0.0004 0.0028 0.0022 0.0002 0.0002 0.0009 0.0001
Vacuole Periphery 0.0025 0.0004 0.0022 0.0005 0.0031 0.0002 0.0039 0.0005 0.0008 0.0001 0.0001 0.0002 0.002 0.0013 0.0001 0.0002 0.001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 46.9059 55.908 46.4249 39.3314 41.509 29.765 38.6951 33.1567 32.7183 41.6417
Translational Efficiency 1.6439 1.3717 1.4006 1.4725 1.4544 1.9999 1.4147 1.6576 1.5181 1.5039

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
Localization
Cell Percentages nucleus (46%), nucleolus (24%), mixed (30%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ski6

Ski6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ski6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available