Standard name
Description Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.13 0.07 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Nucleus 0.58 0.65 0.66 0.95 0.97 0.9 0.89 0.82 0.87 0.82 0.83 0.72 0.82 0.74 0.68 0.63 0.69 0.41 0.5 0.49 0.71 0.57 0.39
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.5 0.36 0.37 0.07 0 0.06 0.06 0.18 0.1 0.14 0.19 0.29 0.17 0.14 0.26 0.25 0.18 0.51 0.4 0.45 0.19 0.21 0.25
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.09 0.05 0 0 0.05 0.17 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 0 2 0 1 0 0 0 0 0 0 0 3 2 3 3 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 0 0 0 2 1 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 4 5 1 3 7 10 8 7 7 3 12 22 46 17 12 17 0 2 0 3 2 3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 4 9 0 0 0 0 0 1
Endosome 1 1 1 0 0 0 2 0 0 2 0 2 0 4 1 3 5 0 3 2 3 0 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 1 0
Mitochondria 1 15 1 0 0 0 3 18 17 8 10 0 0 0 11 10 6 0 1 0 0 0 5
Nucleus 120 138 149 186 250 207 244 213 265 211 257 177 257 260 161 132 188 92 116 135 193 193 134
Nuclear Periphery 0 0 2 1 6 1 2 1 1 0 5 0 0 1 3 1 1 0 0 1 1 2 5
Nucleolus 104 76 85 13 3 13 16 46 32 37 60 72 52 48 61 52 49 114 93 123 53 71 86
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 1 0 1 1 1 1 1 2 4 0 0 1 1 1 1 0 4 1
Vac/Vac Membrane 7 3 4 1 1 7 6 3 5 9 4 3 7 11 14 15 26 10 8 4 13 56 94
Unique Cell Count 206 211 227 196 257 230 273 260 305 257 311 245 313 352 236 211 274 226 233 275 274 339 346
Labelled Cell Count 236 237 247 204 264 236 283 293 330 277 340 268 340 377 270 229 304 226 233 275 274 339 346


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 9.5 12.7 12.6 11.1 9.8 9.2 8.2 8.3 8.9 8.7 13.6 12.5 11.9 10.9 14.2 14.1 13.7 13.5 13.4
Std Deviation (1e-4) 2.7 2.0 2.9 2.4 2.4 2.1 1.9 1.9 2.0 2.1 2.0 3.4 3.4 3.7 3.2 4.6 4.5 3.2 2.7 2.8
Intensity Change (Log2) -0.01 -0.2 -0.37 -0.47 -0.64 -0.61 -0.51 -0.55 0.1 -0.03 -0.1 -0.23 0.16 0.15 0.11 0.09 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.6 1.0 0.6 0.1 0.4 0 1.6 2.5 4.5 2.5 1.9 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.7 3.2 0 0 0 0 0 2.9 2.9 0
Nucleus 7.4 9.1 6.3 6.4 4.1 5.8 4.1 4.5 1.6 4.4 2.1 0.6 -0.7 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -7.5 -10.3 -8.3 -8.8 -4.9 -7.4 -5.8 -4.7 -1.9 -5.5 -6.6 -2.7 -2.9 -4.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0.9 0 0 0 1.2 0 0 0 1.0 2.3 2.7 3.6


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ski6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available