Standard name
Description Subunit of Elongator complex; binds to microtubules via conserved alkaline residues; has two seven-bladed WD40 β propellers; Elongator complex is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0.09 0 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.97 0.94 0.93 0.92 0.87 0.83 0.77 0.74 0.73 0.79 0.99 0.99 1.0 0.91 0.94 0.94 0.98 0.94 0.94 0.84 0.81 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.12 0.11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.14 0.1 0.36 0.38 0.37 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0 0 0.1 0 0.05 0 0 0 0 0 0.09 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 1 0 2 5 4 3 1 2 2 0 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 1 3 2 2 12 12 5 10 13 6 3 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 7 14 6 14 23 15 10 7 11 14 18 0 4 4 21 23 11 0 0 0 0 0 0
Cytoplasm 84 169 267 241 362 435 607 468 206 243 252 333 306 378 480 372 253 247 95 167 281 166 243 308
Endoplasmic Reticulum 0 1 1 4 1 6 0 2 0 1 2 3 3 2 1 10 8 3 0 0 5 15 34 40
Endosome 0 0 0 1 1 1 3 3 0 2 2 2 0 0 0 0 0 1 0 1 1 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 0 0 0 1 1 1
Mitochondria 1 1 1 4 12 36 101 58 98 126 127 111 0 0 1 2 1 0 0 0 2 2 3 4
Nucleus 0 1 0 0 0 3 1 6 0 3 2 1 1 1 0 1 3 1 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 8 8 30 14 21 59 11 15 12 18 1 2 0 37 6 12 0 3 5 7 10 14
Unique Cell Count 85 175 275 256 391 473 697 566 269 328 347 421 310 382 482 410 270 262 97 178 299 197 299 375
Labelled Cell Count 86 181 292 268 424 522 767 625 331 413 427 495 314 387 486 445 295 278 97 178 299 197 299 375


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.2 11.7 11.1 10.3 9.8 7.4 7.0 7.2 5.7 5.5 5.4 5.9 12.9 12.3 11.9 18.4 18.2 17.6 11.4 11.8 12.5
Std Deviation (1e-4) 2.1 2.0 1.4 1.3 1.2 1.1 1.0 1.2 1.3 1.1 1.1 1.1 1.8 1.5 1.4 3.4 3.5 3.2 1.7 1.7 1.9
Intensity Change (Log2) – – – -0.12 -0.18 -0.59 -0.66 -0.64 -0.96 -1.02 -1.04 -0.91 0.22 0.15 0.1 0.72 0.71 0.66 0.04 0.09 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2.5 2.8 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.7 -1.0 -0.1 -2.4 -2.7 -1.5 -1.1 -0.6 -0.5 -4.0 -3.1 -3.7 0 1.6 -0.5
Cytoplasm -1.7 -2.5 -2.8 -4.7 -5.9 -7.1 -7.8 -8.2 -6.7 1.4 1.8 2.9 -3.3 -1.9 -1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.5 4.4 6.5 5.3 10.9 11.4 11.1 9.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.1 2.6 0 0.1 3.8 0.7 1.1 0.4 0.9 0 0 0 3.2 -0.5 1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3959 6.1362 5.3407 5.1354 5.4018 6.2193 4.4387 6.2783 5.2764 4.9472 5.2123 5.3727 4.9044 7.4423 6.6937 6.5153 4.9898 6.8328
Actin 0.0144 0.0022 0.0057 0.088 0.0517 0.0023 0.0236 0.0003 0.0164 0.0106 0.0033 0.0162 0.0216 0.0001 0.0088 0.0124 0.007 0.0061
Bud 0.0005 0.0008 0.0011 0.0011 0.0004 0.0007 0.0009 0.0008 0.0019 0.0012 0.0042 0.0005 0.0017 0.0018 0.0005 0.0013 0.0006 0.0047
Bud Neck 0.0033 0.0004 0.0011 0.0024 0.0007 0.0017 0.0028 0.0002 0.0014 0.0006 0.0006 0.0008 0.0016 0.0001 0.0003 0.0007 0.0008 0.0011
Bud Periphery 0.0005 0.0003 0.0009 0.0012 0.0001 0.001 0.0011 0.0003 0.0021 0.0007 0.0074 0.0005 0.0014 0.0004 0.0002 0.0009 0.0005 0.0037
Bud Site 0.0064 0.0101 0.0038 0.0027 0.0006 0.0004 0.0044 0.0028 0.0066 0.001 0.0082 0.0003 0.0017 0.005 0.001 0.0046 0.0008 0.0004
Cell Periphery 0.001 0.0009 0.0011 0.0011 0.0001 0.0006 0.0006 0.0001 0.0004 0.0001 0.0002 0.0001 0.0004 0.0001 0.0001 0.0002 0.0001 0.0002
Cytoplasm 0.7018 0.8583 0.7891 0.7662 0.619 0.8786 0.8131 0.9563 0.817 0.7629 0.7834 0.8627 0.8094 0.9599 0.9001 0.8666 0.7364 0.9038
Cytoplasmic Foci 0.0327 0.0194 0.0282 0.0128 0.058 0.0156 0.0191 0.0092 0.0243 0.0246 0.0208 0.0191 0.0214 0.0074 0.0195 0.0175 0.0461 0.0135
Eisosomes 0.0004 0 0.0003 0.0099 0.0001 0 0.0002 0 0.0004 0.0001 0 0 0.0002 0 0.0001 0 0.0001 0.0002
Endoplasmic Reticulum 0.0267 0.0088 0.0125 0.0212 0.0065 0.0106 0.0245 0.0037 0.0103 0.0058 0.0064 0.0108 0.0258 0.0018 0.006 0.0113 0.0087 0.0152
Endosome 0.0557 0.0427 0.0389 0.0369 0.2094 0.0331 0.0504 0.0165 0.0646 0.1035 0.0928 0.0586 0.0488 0.0173 0.0314 0.0459 0.1224 0.0342
Golgi 0.0094 0.0043 0.0066 0.0089 0.0135 0.0058 0.0071 0.0012 0.0148 0.0203 0.0235 0.0132 0.0087 0.0007 0.0055 0.0061 0.0207 0.0041
Lipid Particles 0.0095 0.0005 0.0089 0.0029 0.004 0.0001 0.0055 0 0.0038 0.0053 0.0013 0.0009 0.006 0 0.0014 0.0037 0.0227 0.0003
Mitochondria 0.0171 0.0014 0.014 0.0036 0.0021 0.0023 0.0043 0.0003 0.0119 0.0083 0.0159 0.0072 0.011 0.0004 0.0064 0.0032 0.0064 0.0043
None 0.0966 0.0366 0.0605 0.0067 0.0037 0.0374 0.007 0.0023 0.0052 0.0012 0.0009 0.0009 0.0062 0.0011 0.0077 0.0029 0.0025 0.0011
Nuclear Periphery 0.0034 0.0009 0.0025 0.007 0.0017 0.001 0.0139 0.0005 0.0024 0.0091 0.0017 0.0017 0.0079 0.0003 0.0021 0.0026 0.0049 0.0019
Nucleolus 0.0005 0.0001 0.0002 0.0004 0 0.0001 0.0004 0 0.0003 0.0003 0.0005 0 0.0003 0 0.0001 0.0003 0.0003 0.0001
Nucleus 0.0019 0.0011 0.0017 0.0019 0.0009 0.0009 0.005 0.0007 0.0014 0.0018 0.002 0.0007 0.0015 0.0005 0.001 0.0029 0.0032 0.0008
Peroxisomes 0.0058 0.0002 0.0096 0.0009 0.0132 0.0002 0.0026 0.0001 0.0013 0.0017 0.001 0.0005 0.0075 0 0.002 0.0037 0.0031 0.0001
Punctate Nuclear 0.002 0.0002 0.0011 0.0009 0.0041 0.0001 0.0024 0.0001 0.0012 0.0027 0.0003 0.0004 0.0075 0.0001 0.0004 0.0015 0.0021 0.0002
Vacuole 0.0092 0.009 0.0106 0.0198 0.007 0.006 0.0087 0.0037 0.0097 0.0294 0.0201 0.0031 0.0078 0.0023 0.0042 0.0091 0.0073 0.0026
Vacuole Periphery 0.0015 0.0017 0.0015 0.0037 0.0034 0.0017 0.0023 0.0007 0.0027 0.0088 0.0053 0.0016 0.0016 0.0007 0.0012 0.0025 0.0037 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 52.6763 55.4238 51.9497 49.2096 51.6761 37.7382 40.4491 37.8225 38.6696 47.4851
Translational Efficiency 1.1106 1.0454 1.018 1.0798 0.8797 1.0387 1.0413 0.7398 0.7145 0.7237

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘