Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.12 0.13 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0 0.1 0.06 0 0.05 0 0 0 0 0 0.07 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0 0 0.13 0.11 0.16 0 0 0 0.07 0 0
Golgi 0 0 0.19 0.23 0.07 0 0 0 0 0 0 0 0.18 0.34 0.32 0.06 0.22 0.21 0 0.09 0 0.08 0.12 0.06
Mitochondria 0.9 0.93 0.86 0.88 0.89 0.85 0.84 0.72 0.89 0.89 0.91 0.9 0.87 0.77 0.77 0.09 0 0.11 0.85 0.77 0.83 0.7 0.68 0.74
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0.09 0.1 0.08 0.09 0.08 0 0.08 0 0.09 0 0.17 0.09 0.08 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0.08 0 0.07 0.07 0.12 0.1 0 0.11 0 0 0 0.08 0.06 0 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0 0 0 0 0 0 0 0 0 0.09 0 0 0.22 0.16 0.1 0 0 0.09 0.06 0 0.06
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.09 0.06 0.05 0.06 0.07 0.06 0.07 0 0 0 0 0.08 0.08 0.09 0.21 0.19 0.12 0 0 0 0 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 5 2 0 0 0 0 0 0 0 1 2 2 0 0 2 4 2 1 1 3 6
Bud 2 1 4 2 3 4 6 2 3 0 6 6 0 3 0 6 2 6 1 1 1 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 1 4 0 0 1 0 0 0 1 0 0
Cell Periphery 1 10 3 2 1 0 1 1 0 0 0 0 10 9 9 12 14 7 0 0 0 0 0 0
Cytoplasm 0 4 2 8 4 8 7 19 10 7 8 4 6 3 7 4 7 12 0 1 0 0 2 0
Endoplasmic Reticulum 1 3 0 2 0 0 1 2 0 0 0 2 1 5 7 4 3 8 0 1 0 0 1 1
Endosome 7 5 6 2 0 7 11 9 1 1 2 4 6 6 10 13 12 21 5 6 1 6 5 6
Golgi 2 5 28 42 8 4 2 4 0 0 0 1 29 71 74 6 24 27 10 21 5 8 26 14
Mitochondria 236 218 124 159 97 132 171 131 145 149 148 181 140 159 178 9 4 14 234 181 136 71 151 171
Nucleus 1 2 0 1 1 0 1 4 0 2 1 1 0 0 1 7 3 3 0 0 0 0 1 0
Nuclear Periphery 14 7 4 5 3 14 20 14 14 13 6 16 6 19 7 17 10 11 3 0 0 0 1 0
Nucleolus 17 6 1 1 9 6 15 13 19 17 3 22 1 1 1 8 6 5 0 0 0 2 0 1
Peroxisomes 7 4 8 5 0 2 2 1 0 0 1 2 14 8 9 22 17 13 8 10 15 6 9 13
SpindlePole 2 2 1 2 0 3 4 3 0 3 0 4 1 4 1 7 5 11 2 0 0 2 0 1
Vac/Vac Membrane 34 21 8 9 6 11 12 13 6 2 5 4 13 17 20 21 20 15 4 6 0 1 13 7
Unique Cell Count 261 234 145 181 109 155 204 183 163 168 163 202 161 206 232 102 107 130 276 237 164 103 222 232
Labelled Cell Count 324 289 190 245 134 191 253 217 202 194 180 248 228 307 326 137 127 155 276 237 164 103 222 232


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 7.1 9.3 9.5 6.8 7.7 7.6 7.7 6.6 6.3 6.4 6.6 9.3 8.5 8.6 6.8 9.2 8.3 9.3 10.8 10.5
Std Deviation (1e-4) 1.8 1.2 1.4 2.1 2.2 1.8 1.7 1.8 2.0 1.3 1.9 1.6 1.7 1.4 1.8 1.5 3.2 1.7 1.9 2.0 2.3
Intensity Change (Log2) 0.03 -0.45 -0.27 -0.3 -0.28 -0.51 -0.57 -0.54 -0.5 -0.01 -0.14 -0.12 -0.46 -0.02 -0.17 -0.0 0.21 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1.8 0 0 3.1 3.4 0
Cytoplasm 0 0 0 0 3.3 2.2 0 0 0 0 0 0 0 0 3.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.2 0.5 0.3 0 0 0 0 -0.2 0 0.1 2.5 2.2 3.3
Golgi 0.9 -2.7 -4.7 -6.0 -5.2 -5.9 -6.0 -5.9 -6.2 -0.3 3.1 2.7 -3.0 0.6 0.3
Mitochondria 0.6 0.8 -0.1 -0.4 -3.0 0.9 0.8 1.4 1.2 0.4 -1.9 -2.1 -11.9 -12.8 -12.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.3 2.6 1.9 2.2 1.9 0 2.0 0 2.4 0 3.9 2.3 2.1
Nucleolus 0 0 0 2.9 2.9 3.9 3.6 0 3.8 0 0 0 0 0 0
Peroxisomes -1.3 0 0 0 0 0 0 0 0 1.1 -0.7 -0.7 3.8 2.7 1.4
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.1
Vacuole -0.2 0 0.6 0.1 0.6 -0.8 0 -1.1 -1.8 0.9 1.0 1.1 3.6 3.3 1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.5037 6.5248 6.5375 6.2323 6.2133 6.1993 11.7622 12.0249 11.8114 12.5841 11.2889 11.7206 8.9003 8.5591 8.3349 7.1309 5.5366 8.3679
Actin 0.0125 0.0024 0.0127 0.0007 0.0125 0.0091 0.0093 0.0039 0.0018 0 0.0009 0.0007 0.0216 0.0054 0.0238 0.008 0.0051 0.0077
Bud 0.0002 0.0002 0.0002 0.0001 0.0005 0.0002 0.0001 0.0001 0.0001 0 0 0 0.0013 0.0003 0.0008 0.0002 0.0011 0.0001
Bud Neck 0.0001 0 0.0005 0 0.0004 0.0001 0.0001 0 0 0 0 0 0.0027 0.0001 0.0072 0 0.0002 0.0001
Bud Periphery 0.0007 0.0003 0.0004 0.0002 0.001 0.0004 0.0002 0.0001 0.0001 0 0.0001 0 0.0017 0.0004 0.0011 0.0006 0.0022 0.0001
Bud Site 0.0008 0.0003 0.0014 0 0.001 0.0002 0.0012 0.0001 0.0001 0 0.0001 0 0.0141 0.002 0.0173 0.0002 0.0039 0.0002
Cell Periphery 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0001 0 0 0 0 0.0001 0.0007 0.0001 0.0016 0.0002 0.0003 0
Cytoplasm 0.0028 0.0001 0.0015 0 0.0012 0.001 0 0 0 0 0.0001 0 0.0087 0 0.0338 0 0.0002 0.0002
Cytoplasmic Foci 0.0094 0.0026 0.0024 0.0001 0.0183 0.0054 0.0014 0.0015 0.0027 0 0.0011 0.0002 0.0189 0.0015 0.0039 0.0014 0.0047 0.0062
Eisosomes 0.0006 0.0001 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0005 0.0002 0.0009 0.0015 0.0003 0.0001
Endoplasmic Reticulum 0.0005 0.0001 0.0015 0 0.0011 0.0008 0 0 0 0 0.0004 0 0.0007 0 0.0038 0 0.0003 0.0001
Endosome 0.0098 0.0059 0.009 0.0004 0.0279 0.0094 0.0035 0.0032 0.0055 0.0002 0.0021 0.0007 0.0111 0.0082 0.002 0.0007 0.0161 0.0118
Golgi 0.0182 0.0237 0.0096 0.0008 0.0558 0.018 0.0113 0.0186 0.011 0.001 0.0057 0.0045 0.0146 0.0114 0.0026 0.002 0.0134 0.0106
Lipid Particles 0.0327 0.0123 0.0075 0.0001 0.0214 0.01 0.0112 0.0016 0.0069 0.0001 0.0225 0.0014 0.0167 0.0032 0.0039 0.0031 0.0071 0.0038
Mitochondria 0.8771 0.9324 0.9115 0.9968 0.7922 0.9216 0.9374 0.964 0.9589 0.9977 0.9592 0.9842 0.7906 0.9347 0.8732 0.9436 0.9309 0.9462
None 0.0015 0 0.0042 0 0.0012 0.0003 0 0 0 0 0.0003 0 0.0247 0 0.0012 0.0001 0.0003 0
Nuclear Periphery 0.0055 0 0.0094 0 0.0071 0.0014 0 0 0.0001 0 0.0014 0 0.0028 0 0.0005 0 0.0003 0.0001
Nucleolus 0.0009 0.0001 0.0006 0 0.0006 0.0001 0.0022 0.0001 0 0 0.0001 0 0.0093 0.0001 0.0015 0.0001 0.0007 0
Nucleus 0.0056 0.0001 0.0082 0 0.0023 0.0003 0.0002 0 0 0 0.0001 0 0.01 0 0.0005 0 0.0007 0
Peroxisomes 0.015 0.0121 0.0064 0.0003 0.0061 0.0156 0.0187 0.0061 0.0098 0.0007 0.0035 0.0042 0.0435 0.0288 0.0173 0.0378 0.0062 0.0114
Punctate Nuclear 0.0024 0 0.0045 0 0.0451 0.0007 0 0 0 0 0.0003 0 0.0017 0 0.0002 0.0001 0.0002 0.0001
Vacuole 0.0008 0.0024 0.0037 0.0001 0.0013 0.0005 0.0008 0.0001 0.0005 0 0.0003 0.001 0.0024 0.0013 0.0024 0.0002 0.0032 0.0003
Vacuole Periphery 0.0025 0.0048 0.0045 0.0003 0.0025 0.0047 0.0021 0.0004 0.0021 0.0002 0.0018 0.0028 0.0019 0.0024 0.0006 0.0004 0.0028 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.7801 23.1372 24.3978 38.9793 36.2068 35.3983 34.1676 35.7339 44.0293 44.6849
Translational Efficiency 0.9124 1.2362 1.2564 1.0623 0.8174 1.0219 1.0432 1.0634 0.998 0.9375

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
504 953 2224 1907 1289 2005 198 92 1793 2958 2422 1999

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 884.68 1092.17 1360.80 1259.29 945.89 1122.99 1290.06 1427.77 928.68 1113.06 1355.02 1267.04
Standard Deviation 161.00 210.40 236.97 205.36 165.06 194.73 214.66 220.55 166.22 200.43 236.02 209.08
Intensity Change Log 2 0.303970 0.621227 0.509383 0.247601 0.447694 0.594019 0.275118 0.534168 0.553736

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001786 0.005419 0.002231 0.006987 0.001648 0.003241 0.003656 0.013646 0.001687 0.003943 0.002347 0.007293
Bud Neck 0.001289 0.001447 0.000810 0.000887 0.001740 0.000871 0.010376 0.004512 0.001613 0.001056 0.001592 0.001054
Bud Site 0.002977 0.002332 0.001116 0.004072 0.001586 0.002135 0.009981 0.003784 0.001977 0.002198 0.001841 0.004059
Cell Periphery 0.000137 0.000136 0.000062 0.000474 0.000144 0.000146 0.000146 0.006455 0.000142 0.000143 0.000069 0.000749
Cytoplasm 0.000507 0.000656 0.000257 0.000616 0.000378 0.000287 0.000870 0.000521 0.000415 0.000406 0.000308 0.000612
Cytoplasmic Foci 0.013490 0.026340 0.014665 0.005586 0.025344 0.010549 0.044685 0.031671 0.022012 0.015637 0.017119 0.006787
Eisosomes 0.000074 0.000178 0.000127 0.000197 0.000114 0.000120 0.000111 0.001056 0.000103 0.000139 0.000126 0.000237
Endoplasmic Reticulum 0.000227 0.000256 0.000157 0.000218 0.000358 0.000104 0.000420 0.002316 0.000321 0.000153 0.000178 0.000314
Endosome 0.013675 0.017696 0.004827 0.005427 0.013894 0.009698 0.028978 0.012978 0.013833 0.012275 0.006801 0.005775
Golgi 0.024624 0.049413 0.043197 0.027402 0.028789 0.029128 0.048827 0.040339 0.027618 0.035663 0.043658 0.027997
Lipid Particles 0.007097 0.008452 0.004225 0.000578 0.012784 0.005040 0.006779 0.003462 0.011185 0.006139 0.004434 0.000710
Mitochondria 0.902984* 0.843445* 0.895502* 0.925242* 0.869904* 0.918454* 0.735062* 0.816391* 0.879202* 0.894288* 0.882386* 0.920232*
Mitotic Spindle 0.002551 0.003404 0.002668 0.002797 0.003505 0.000719 0.033159 0.002200 0.003237 0.001584 0.005160 0.002769
None 0.000344 0.000532 0.001497 0.000464 0.000609 0.000420 0.000731 0.001247 0.000535 0.000456 0.001435 0.000500
Nuclear Periphery 0.000072 0.000178 0.000924 0.000076 0.000399 0.000048 0.004033 0.000317 0.000307 0.000090 0.001178 0.000087
Nuclear Periphery Foci 0.000138 0.000590 0.000407 0.000093 0.001187 0.000073 0.002669 0.000408 0.000892 0.000240 0.000592 0.000107
Nucleolus 0.000071 0.000212 0.000143 0.000208 0.000639 0.000202 0.001068 0.000202 0.000479 0.000205 0.000219 0.000207
Nucleus 0.000059 0.000090 0.000045 0.000047 0.000424 0.000247 0.000364 0.000144 0.000321 0.000196 0.000071 0.000051
Peroxisomes 0.023061 0.032918 0.004976 0.008436 0.028644 0.013584 0.034307 0.047818 0.027075 0.019813 0.007374 0.010249
Vacuole 0.001771 0.002835 0.005805 0.003806 0.003289 0.002656 0.016532 0.005594 0.002862 0.002714 0.006682 0.003889
Vacuole Periphery 0.003066 0.003473 0.016360 0.006387 0.004622 0.002279 0.017246 0.004938 0.004185 0.002664 0.016432 0.006321

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.77 -0.64 -4.72 -1.05 -4.75 -2.93 -1.69 -3.30 -2.84 -2.62 -4.06 -1.56 -6.00 -3.24 -5.10
Bud Neck -0.34 1.13 0.88 1.79 -0.44 1.62 -3.61 -1.99 -2.76 2.21 1.37 0.01 1.31 -0.08 1.85
Bud Site 0.50 1.57 -0.88 -1.77 -3.37 -0.69 -3.02 -1.51 -1.08 2.04 -0.34 0.18 -2.38 -2.11 -2.58
Cell Periphery 0.06 4.39 -1.97 -1.98 -2.42 -0.03 -0.04 -1.25 -1.25 -1.25 -0.00 3.17 -2.13 -2.13 -2.40
Cytoplasm -0.75 1.53 -0.66 0.26 -4.97 1.10 -2.55 -1.22 -2.34 1.76 0.10 1.41 -2.17 -2.62 -4.34
Cytoplasmic Foci -4.41 -0.52 3.71 9.66 8.21 6.93 -2.58 -0.84 -2.89 1.27 3.61 2.62 9.18 8.04 8.29
Eisosomes -6.00 -3.46 -3.98 -0.66 -2.46 -0.33 0.19 -3.26 -3.25 -3.28 -2.61 -1.74 -4.19 -3.21 -3.65
Endoplasmic Reticulum -0.41 1.32 0.15 0.63 -1.63 3.71 -0.65 -1.22 -1.38 -1.18 3.12 2.69 0.09 -1.95 -1.65
Endosome -1.49 4.13 3.84 6.93 -0.90 2.84 -2.75 0.21 -0.78 2.37 1.18 5.86 7.02 6.72 1.29
Golgi -4.70 -4.12 -0.64 5.11 4.76 -0.10 -2.50 -1.33 -1.30 0.75 -2.94 -5.17 -0.13 2.71 4.91
Lipid Particles -0.54 1.39 3.21 5.27 9.36 4.65 2.58 4.94 1.07 1.51 3.61 5.45 8.83 7.19 9.47
Mitochondria 4.54 0.70 -2.05 -8.41 -4.78 -5.71 5.30 1.84 3.58 -2.20 -2.09 -0.41 -5.60 -4.29 -5.96
Mitotic Spindle -0.80 -0.14 -0.27 0.65 -0.16 3.59 -3.69 1.44 -2.90 3.86 2.54 -1.90 0.60 -2.07 2.47
None -3.18 -16.67 -3.03 1.30 16.28 1.76 -0.90 -2.83 -3.67 -2.17 1.01 -11.37 0.59 -0.74 15.52
Nuclear Periphery -1.57 -6.23 -0.27 1.47 6.17 2.78 -2.64 0.49 -2.41 2.70 2.32 -4.55 2.38 0.10 6.43
Nuclear Periphery Foci -1.55 -3.28 0.89 1.71 4.10 2.90 -1.67 1.95 -2.86 2.79 2.23 1.03 2.83 1.35 4.98
Nucleolus -2.14 -2.60 -0.87 0.03 -0.40 2.13 -0.90 2.37 0.02 1.90 1.87 1.99 1.41 -0.01 0.08
Nucleus -1.20 0.63 0.41 1.36 -0.06 0.70 0.30 1.42 0.54 1.97 0.71 1.92 2.05 1.19 0.76
Peroxisomes -1.80 4.16 3.32 6.77 -2.56 4.69 -0.72 -1.37 -2.49 -0.85 2.58 7.74 6.36 4.87 -1.82
Vacuole -1.50 -5.12 -2.54 -1.34 2.25 0.89 -4.50 -0.87 -1.10 2.81 0.27 -5.47 -1.51 -1.73 3.21
Vacuole Periphery -0.41 -10.10 -3.08 -2.54 6.94 2.46 -3.59 -0.21 -2.09 3.40 2.04 -9.55 -2.01 -4.14 7.39