Standard name | |
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Human Ortholog | |
Description | Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 5 | 6 | 2 | 6 | 1 | 0 | 3 | 1 | 0 | 2 | 0 | 4 | 6 | 0 | – | 0 | – | 0 | 3 | 5 | 0 | 5 | 3 |
Bud | 1 | 6 | 8 | 4 | 5 | 8 | 11 | 15 | 16 | 23 | 16 | 31 | 3 | 4 | 0 | – | 1 | – | 0 | 2 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 3 | 0 | 1 | 1 | 2 | 3 | 1 | 1 | 0 | 0 | 1 | 1 | 2 | – | 0 | – | 1 | 1 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 10 | 1 | 10 | 9 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – |
Cell Periphery | 2 | 7 | 4 | 4 | 5 | 6 | 2 | 4 | 1 | 3 | 2 | 2 | 4 | 1 | 2 | – | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 30 | 18 | 22 | 32 | 32 | 29 | 44 | 19 | 25 | 17 | 19 | 15 | 37 | 87 | – | 9 | – | 1 | 7 | 0 | 1 | 3 | 3 |
Endoplasmic Reticulum | 7 | 4 | 0 | 0 | 8 | 5 | 1 | 0 | 4 | 2 | 1 | 1 | 7 | 6 | 3 | – | 1 | – | 2 | 1 | 0 | 0 | 3 | 2 |
Endosome | 9 | 12 | 12 | 16 | 53 | 30 | 37 | 30 | 1 | 11 | 3 | 12 | 9 | 14 | 16 | – | 9 | – | 16 | 18 | 17 | 2 | 8 | 18 |
Golgi | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | – | 4 | 7 | 4 | 0 | 4 | 1 |
Mitochondria | 107 | 153 | 36 | 88 | 92 | 212 | 264 | 206 | 357 | 416 | 330 | 413 | 49 | 20 | 10 | – | 5 | – | 124 | 173 | 134 | 33 | 121 | 149 |
Nucleus | 0 | 1 | 0 | 0 | 2 | 16 | 13 | 14 | 10 | 9 | 4 | 9 | 0 | 1 | 0 | – | 2 | – | 0 | 2 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 4 | 2 | 4 | 2 | 0 | 0 | 0 | – | 0 | – | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 2 | 3 | 3 | 3 | 4 | 0 | 0 | 0 | – | 0 | – | 1 | 1 | 1 | 0 | 0 | 1 |
Peroxisomes | 7 | 12 | 16 | 25 | 23 | 9 | 19 | 15 | 5 | 8 | 4 | 10 | 2 | 2 | 13 | – | 8 | – | 9 | 5 | 12 | 3 | 3 | 7 |
SpindlePole | 11 | 4 | 30 | 15 | 36 | 27 | 52 | 49 | 26 | 44 | 21 | 45 | 7 | 9 | 8 | – | 5 | – | 3 | 1 | 3 | 1 | 2 | 4 |
Vac/Vac Membrane | 17 | 23 | 8 | 8 | 20 | 25 | 43 | 42 | 11 | 27 | 12 | 24 | 7 | 6 | 7 | – | 6 | – | 3 | 8 | 2 | 4 | 11 | 9 |
Unique Cell Count | 141 | 197 | 112 | 144 | 219 | 304 | 383 | 335 | 392 | 476 | 360 | 456 | 87 | 95 | 140 | 34 | 173 | 239 | 191 | 52 | 172 | 209 | ||
Labelled Cell Count | 171 | 260 | 142 | 184 | 284 | 376 | 481 | 429 | 469 | 575 | 429 | 581 | 108 | 107 | 149 | 47 | 173 | 239 | 191 | 52 | 172 | 209 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 4.8 | 5.4 | 5.1 | 5.5 | 4.6 | 4.7 | 5.1 | 3.8 | 4.3 | 4.0 | 4.2 | 4.7 | 5.2 | 5.1 | – | 5.5 | – | – | 5.8 | 5.8 |
Std Deviation (1e-4) | 0.8 | 1.6 | 1.4 | 1.4 | 0.9 | 1.2 | 1.4 | 1.9 | 1.0 | 1.1 | 0.9 | 1.7 | 0.8 | 1.1 | 1.1 | – | 1.9 | – | – | 2.4 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.03 | -0.25 | -0.21 | -0.09 | -0.5 | -0.33 | -0.44 | -0.36 | -0.2 | -0.05 | -0.09 | – | 0.04 | – | – | 0.09 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.268 | 1.5989 | 1.3105 | 1.0927 | 0.4333 | 1.1105 | 2.1647 | 2.0962 | 2.0621 | 1.8253 | 1.3512 | 1.9449 | -0.7667 | -0.0405 | -0.4532 | -0.3037 | -0.6397 | -0.5032 |
Actin | 0.0548 | 0.0125 | 0.0381 | 0.0381 | 0.0397 | 0.019 | 0.0432 | 0.0187 | 0.0439 | 0.0188 | 0.0035 | 0.0314 | 0.033 | 0.0216 | 0.0432 | 0.0099 | 0.0264 | 0.0165 |
Bud | 0.001 | 0.0035 | 0.0034 | 0.0022 | 0.0021 | 0.0011 | 0.0013 | 0.0026 | 0.0034 | 0.0078 | 0.001 | 0.0019 | 0.001 | 0.0017 | 0.0021 | 0.0059 | 0.0075 | 0.0007 |
Bud Neck | 0.0039 | 0.0018 | 0.0057 | 0.0365 | 0.0024 | 0.0129 | 0.01 | 0.0007 | 0.0041 | 0.046 | 0.0016 | 0.0107 | 0.0018 | 0.0019 | 0.0087 | 0.0025 | 0.0019 | 0.0015 |
Bud Periphery | 0.0026 | 0.007 | 0.0107 | 0.0062 | 0.0067 | 0.0047 | 0.003 | 0.006 | 0.0128 | 0.04 | 0.0025 | 0.0109 | 0.0017 | 0.0022 | 0.0035 | 0.0139 | 0.0187 | 0.0015 |
Bud Site | 0.0166 | 0.0494 | 0.0256 | 0.0114 | 0.0027 | 0.0017 | 0.0169 | 0.0096 | 0.0172 | 0.0113 | 0.0043 | 0.0027 | 0.0039 | 0.0433 | 0.0145 | 0.0094 | 0.0068 | 0.0009 |
Cell Periphery | 0.0047 | 0.0018 | 0.0069 | 0.0034 | 0.0018 | 0.0027 | 0.0055 | 0.0024 | 0.0052 | 0.0323 | 0.0008 | 0.0173 | 0.001 | 0.0014 | 0.0009 | 0.0013 | 0.0006 | 0.0005 |
Cytoplasm | 0.0195 | 0.0274 | 0.0184 | 0.0119 | 0.0053 | 0.0269 | 0.0396 | 0.0259 | 0.0266 | 0.0357 | 0.078 | 0.0193 | 0.0301 | 0.0393 | 0.0282 | 0.0231 | 0.012 | 0.0186 |
Cytoplasmic Foci | 0.0887 | 0.1337 | 0.0726 | 0.1304 | 0.0312 | 0.082 | 0.0605 | 0.0969 | 0.055 | 0.0881 | 0.0588 | 0.0812 | 0.0955 | 0.113 | 0.0792 | 0.1103 | 0.0951 | 0.0807 |
Eisosomes | 0.0032 | 0.0008 | 0.0023 | 0.0051 | 0.0011 | 0.0015 | 0.0104 | 0.0024 | 0.0029 | 0.0028 | 0.0003 | 0.0018 | 0.0008 | 0.0005 | 0.0012 | 0.0003 | 0.0003 | 0.0003 |
Endoplasmic Reticulum | 0.0058 | 0.0015 | 0.003 | 0.0009 | 0.0046 | 0.0031 | 0.0127 | 0.0016 | 0.0034 | 0.0016 | 0.0012 | 0.0028 | 0.0096 | 0.0025 | 0.0072 | 0.0055 | 0.0039 | 0.0029 |
Endosome | 0.1172 | 0.1144 | 0.0701 | 0.0927 | 0.0528 | 0.06 | 0.0897 | 0.1299 | 0.109 | 0.1038 | 0.1042 | 0.0699 | 0.1861 | 0.1924 | 0.1127 | 0.123 | 0.2091 | 0.1671 |
Golgi | 0.0456 | 0.0715 | 0.0469 | 0.0518 | 0.0487 | 0.0523 | 0.0658 | 0.0859 | 0.066 | 0.1061 | 0.0729 | 0.0709 | 0.1146 | 0.1335 | 0.0755 | 0.0788 | 0.0867 | 0.1233 |
Lipid Particles | 0.1086 | 0.0262 | 0.0205 | 0.01 | 0.0174 | 0.0398 | 0.0651 | 0.0446 | 0.0299 | 0.075 | 0.0076 | 0.0366 | 0.0854 | 0.0304 | 0.0242 | 0.0467 | 0.0147 | 0.0318 |
Mitochondria | 0.288 | 0.4241 | 0.5198 | 0.5032 | 0.7272 | 0.5572 | 0.3716 | 0.3689 | 0.4629 | 0.2891 | 0.6199 | 0.5004 | 0.314 | 0.3069 | 0.5115 | 0.4541 | 0.4319 | 0.4564 |
None | 0.0839 | 0.0129 | 0.0521 | 0.0469 | 0.0062 | 0.0516 | 0.0869 | 0.0727 | 0.088 | 0.0469 | 0.0078 | 0.052 | 0.0442 | 0.0409 | 0.0156 | 0.0104 | 0.0073 | 0.025 |
Nuclear Periphery | 0.0118 | 0.0004 | 0.0009 | 0.0002 | 0.0057 | 0.0003 | 0.0104 | 0.0003 | 0.0011 | 0.0005 | 0.0002 | 0.0005 | 0.0141 | 0.001 | 0.0009 | 0.0009 | 0.0079 | 0.0025 |
Nucleolus | 0.0027 | 0.0015 | 0.0015 | 0.0007 | 0.0009 | 0.0011 | 0.0058 | 0.0007 | 0.0011 | 0.0024 | 0.0003 | 0.0004 | 0.0014 | 0.0007 | 0.0005 | 0.0015 | 0.0012 | 0.0003 |
Nucleus | 0.004 | 0.0014 | 0.0013 | 0.0005 | 0.0017 | 0.0008 | 0.0225 | 0.0005 | 0.0012 | 0.0012 | 0.0005 | 0.0005 | 0.0031 | 0.0009 | 0.0008 | 0.001 | 0.0019 | 0.0006 |
Peroxisomes | 0.0913 | 0.0926 | 0.0865 | 0.042 | 0.0336 | 0.0738 | 0.0521 | 0.1196 | 0.0494 | 0.0743 | 0.0281 | 0.0804 | 0.038 | 0.0436 | 0.0524 | 0.0833 | 0.0315 | 0.0461 |
Punctate Nuclear | 0.0082 | 0.0012 | 0.0008 | 0.0007 | 0.002 | 0.0012 | 0.009 | 0.0005 | 0.0007 | 0.0011 | 0.0003 | 0.0005 | 0.0019 | 0.0006 | 0.0056 | 0.003 | 0.0185 | 0.0004 |
Vacuole | 0.0279 | 0.0109 | 0.0083 | 0.0034 | 0.0034 | 0.0038 | 0.0116 | 0.0076 | 0.0116 | 0.0107 | 0.0036 | 0.0044 | 0.0121 | 0.0147 | 0.0075 | 0.0084 | 0.0084 | 0.0102 |
Vacuole Periphery | 0.01 | 0.0035 | 0.0046 | 0.002 | 0.0028 | 0.0024 | 0.0063 | 0.0022 | 0.0045 | 0.0046 | 0.0025 | 0.0037 | 0.0065 | 0.007 | 0.0038 | 0.0068 | 0.0076 | 0.0121 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.6378 | 11.1834 | 13.2777 | 16.1285 | 15.4091 | 5.0013 | 10.7422 | 10.303 | 15.935 | 20.6183 |
Translational Efficiency | 0.4047 | 0.4249 | 0.5555 | 0.5629 | 0.3591 | 1.6775 | 0.8374 | 1.1962 | 0.5274 | 0.393 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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