Standard name
Description Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0.06 0 – 0 – 0 0 0 0 0 0
Bud 0 0 0.07 0 0 0 0 0 0 0.05 0 0.07 0 0 0 – 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 0.05 0.15 0.16 0.15 0.15 0.11 0.08 0.13 0.05 0.05 0.05 0 0.17 0.39 0.62 – 0.26 – 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0 – 0 – 0 0 0 0 0 0
Endosome 0.06 0.06 0.11 0.11 0.24 0.1 0.1 0.09 0 0 0 0 0.1 0.15 0.11 – 0.26 – 0.1 0.08 0.09 0.05 0.05 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Mitochondria 0.76 0.78 0.32 0.61 0.42 0.7 0.69 0.61 0.91 0.87 0.92 0.91 0.56 0.21 0.07 – 0.15 – 0.72 0.73 0.71 0.64 0.71 0.72
Nucleus 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 – 0.06 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0.05 0.06 0.14 0.17 0.11 0 0.05 0 0 0 0 0 0 0 0.09 – 0.24 – 0.05 0 0.06 0.08 0 0
SpindlePole 0.08 0 0.27 0.1 0.16 0.09 0.14 0.15 0.07 0.09 0.06 0.1 0.08 0.09 0.06 – 0.15 – 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.12 0.07 0.06 0.09 0.08 0.11 0.13 0 0.06 0 0.05 0.08 0.06 0.05 – 0.18 – 0 0 0 0.08 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 5 6 2 6 1 0 3 1 0 2 0 4 6 0 – 0 – 0 3 5 0 5 3
Bud 1 6 8 4 5 8 11 15 16 23 16 31 3 4 0 – 1 – 0 2 0 0 0 1
Bud Neck 0 0 3 0 1 1 2 3 1 1 0 0 1 1 2 – 0 – 1 1 0 0 1 1
Bud Site 0 0 0 0 1 2 2 2 10 1 10 9 0 0 0 – 0 – – – – – – –
Cell Periphery 2 7 4 4 5 6 2 4 1 3 2 2 4 1 2 – 1 – 0 0 0 0 0 0
Cytoplasm 7 30 18 22 32 32 29 44 19 25 17 19 15 37 87 – 9 – 1 7 0 1 3 3
Endoplasmic Reticulum 7 4 0 0 8 5 1 0 4 2 1 1 7 6 3 – 1 – 2 1 0 0 3 2
Endosome 9 12 12 16 53 30 37 30 1 11 3 12 9 14 16 – 9 – 16 18 17 2 8 18
Golgi 0 2 1 0 0 0 0 0 0 0 0 0 0 0 1 – 0 – 4 7 4 0 4 1
Mitochondria 107 153 36 88 92 212 264 206 357 416 330 413 49 20 10 – 5 – 124 173 134 33 121 149
Nucleus 0 1 0 0 2 16 13 14 10 9 4 9 0 1 0 – 2 – 0 2 0 0 0 1
Nuclear Periphery 0 1 0 0 0 1 1 0 4 2 4 2 0 0 0 – 0 – 1 0 0 0 0 1
Nucleolus 1 0 0 0 0 1 5 2 3 3 3 4 0 0 0 – 0 – 1 1 1 0 0 1
Peroxisomes 7 12 16 25 23 9 19 15 5 8 4 10 2 2 13 – 8 – 9 5 12 3 3 7
SpindlePole 11 4 30 15 36 27 52 49 26 44 21 45 7 9 8 – 5 – 3 1 3 1 2 4
Vac/Vac Membrane 17 23 8 8 20 25 43 42 11 27 12 24 7 6 7 – 6 – 3 8 2 4 11 9
Unique Cell Count 141 197 112 144 219 304 383 335 392 476 360 456 87 95 140 34 173 239 191 52 172 209
Labelled Cell Count 171 260 142 184 284 376 481 429 469 575 429 581 108 107 149 47 173 239 191 52 172 209


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.8 5.4 5.1 5.5 4.6 4.7 5.1 3.8 4.3 4.0 4.2 4.7 5.2 5.1 – 5.5 – – 5.8 5.8
Std Deviation (1e-4) 0.8 1.6 1.4 1.4 0.9 1.2 1.4 1.9 1.0 1.1 0.9 1.7 0.8 1.1 1.1 – 1.9 – – 2.4 1.3
Intensity Change (Log2) – – – -0.1 0.03 -0.25 -0.21 -0.09 -0.5 -0.33 -0.44 -0.36 -0.2 -0.05 -0.09 – 0.04 – – 0.09 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –