Standard name
Human Ortholog
Description Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0.06 0.07 0.1 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.05 0.08 0 0.11 0 0 0 0 0 0 0 0.07 0.2 0.25 0.09 0.12 0.05 0 0.06 0.06 0.09 0.09 0.08
Golgi 0 0 0.12 0.08 0.11 0 0 0 0 0 0 0 0.15 0.15 0.17 0.23 0.18 0.13 0.06 0 0 0 0 0
Mitochondria 0.97 0.65 0.57 0.7 0.72 0.69 0.81 0.78 0.89 0.88 0.91 0.85 0.56 0.42 0.26 0.12 0.05 0.12 0.79 0.58 0.66 0.61 0.59 0.59
Nucleus 0 0.07 0.08 0.08 0 0.06 0 0.07 0.07 0 0.05 0.06 0.07 0.09 0.07 0.14 0.16 0.15 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.05 0 0 0.05 0.08 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.06 0 0.09 0 0.07 0.1 0.06 0.06 0 0.06 0.08 0.06 0.06 0 0.3 0.3 0.33 0 0 0 0 0.05 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.1 0 0 0 0.06 0 0.06 0.05 0.05 0.07
SpindlePole 0 0 0.11 0.18 0.1 0.11 0.1 0.1 0 0 0 0.07 0.11 0.12 0.13 0.12 0.12 0.09 0 0 0 0.05 0.08 0.05
Vac/Vac Membrane 0.09 0.24 0.14 0.06 0.11 0.11 0.05 0 0 0.07 0 0.06 0.16 0.19 0.2 0.16 0.11 0.13 0 0.13 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 4 0 0 1 1 0 0 0 0 15 2 1 4 9 5 1 1 1 3 6 12
Bud 0 4 0 8 4 3 13 6 4 7 5 7 8 2 2 4 3 2 0 1 0 1 5 8
Bud Neck 0 0 2 2 0 1 2 1 1 1 0 0 0 3 0 0 0 1 0 1 0 3 5 16
Bud Site 0 0 1 1 3 1 11 4 4 5 4 4 1 0 0 0 1 2
Cell Periphery 0 1 5 2 3 2 1 2 2 2 1 1 13 8 8 11 14 4 0 0 0 0 0 0
Cytoplasm 0 4 3 7 10 10 18 8 6 10 7 8 15 25 32 16 21 18 0 1 0 0 0 3
Endoplasmic Reticulum 1 1 2 0 4 1 1 3 0 2 0 4 2 3 10 4 5 4 0 0 2 0 2 3
Endosome 0 7 7 7 31 8 8 13 2 8 1 2 27 105 131 20 24 9 2 10 9 36 59 64
Golgi 0 1 11 14 30 1 1 0 0 0 0 0 55 80 89 52 36 22 5 6 1 14 16 25
Mitochondria 93 96 52 126 202 154 330 269 221 312 243 246 206 219 134 27 10 20 73 95 92 235 375 459
Nucleus 1 10 7 15 12 14 14 23 17 9 14 18 27 45 36 31 33 25 0 6 3 7 10 8
Nuclear Periphery 4 4 0 1 7 12 13 14 13 28 11 14 7 6 3 1 3 2 0 1 0 6 6 9
Nucleolus 11 9 4 16 11 16 41 20 14 16 16 23 23 32 21 69 62 55 0 6 5 15 32 31
Peroxisomes 1 3 3 1 3 2 6 6 5 3 2 3 17 29 50 7 7 5 5 3 8 20 31 54
SpindlePole 0 4 10 33 28 24 41 35 9 15 6 20 41 64 67 27 24 15 0 5 4 18 51 39
Vac/Vac Membrane 9 35 13 11 30 24 21 11 11 24 12 16 59 102 104 36 23 21 1 21 7 15 26 33
Unique Cell Count 96 147 92 180 282 222 405 347 249 356 268 288 365 525 516 228 204 166 93 165 141 385 639 783
Labelled Cell Count 120 181 121 248 378 273 522 416 309 442 322 366 516 725 688 309 275 210 93 165 141 385 639 783


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 6.0 6.8 6.3 6.9 5.8 5.4 5.7 5.2 5.5 4.8 5.2 7.2 7.0 6.9 10.1 10.4 10.1 6.4 6.5 6.9
Std Deviation (1e-4) 0.9 1.3 1.8 1.6 1.5 1.4 1.6 1.3 1.7 1.6 1.4 1.3 1.3 1.2 1.3 3.2 3.5 3.2 1.3 1.4 1.5
Intensity Change (Log2) -0.1 0.02 -0.23 -0.33 -0.24 -0.38 -0.3 -0.48 -0.39 0.09 0.04 0.03 0.58 0.61 0.57 -0.08 -0.06 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.5 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 1.1 1.3 2.1 2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.9 0 0 0 0 0 0 0 -0.1 2.8 3.8 0.3 1.1 -0.7
Golgi -1.1 -0.4 0 0 0 0 0 0 0 0.8 0.8 1.3 2.2 1.2 0.3
Mitochondria 2.2 2.7 2.2 5.1 4.0 6.6 6.8 0 5.9 0 -2.6 -5.9 -8.4 -10.1 -7.6
Nucleus 0.2 0 -0.4 0 -0.3 -0.3 0 -0.8 -0.5 -0.1 0.3 -0.2 1.5 2.0 1.7
Nuclear Periphery 0 0 0 0 0 0 2.8 0 0 0 0 0 0 0 0
Nucleolus 1.4 0 0.9 1.7 0.5 0 0 0 1.2 0.7 0.7 0 5.0 5.0 5.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 2.0 0 0 0
SpindlePole 1.6 -0.3 0 -0.2 -0.2 -2.6 -2.5 0 -1.2 0.1 0.4 0.6 0.2 0.2 -0.5
Vacuole -2.2 -0.9 -0.8 -3.1 -4.1 -3.1 -2.3 -3.1 -2.7 0.5 1.2 1.4 0.4 -0.7 -0.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3217 5.7335 4.986 4.8846 4.0302 4.6385 6.6309 7.3263 6.8317 6.9124 6.0457 7.1572 7.4417 6.7532 6.6504 6.0197 6.0591 7.1924
Actin 0.0722 0.0101 0.0437 0.0202 0.1825 0.0323 0.0108 0.0144 0.0039 0.0002 0.0044 0.0155 0.029 0.0267 0.0313 0.0536 0.0193 0.015
Bud 0.0009 0.0037 0.0034 0.0003 0.0002 0.0003 0.0005 0.0037 0.002 0.003 0.0001 0.0003 0.0015 0.0031 0.0012 0.0009 0.0005 0.0005
Bud Neck 0.0044 0.0009 0.0037 0.0005 0.0003 0.0137 0.0004 0.0007 0.0004 0.0003 0.0001 0.0003 0.0028 0.0006 0.0023 0.0003 0.0003 0.0006
Bud Periphery 0.0013 0.0057 0.0052 0.0004 0.0002 0.0005 0.001 0.0061 0.003 0.0038 0.0003 0.0006 0.0016 0.0032 0.0016 0.0011 0.0008 0.0008
Bud Site 0.0108 0.0144 0.0069 0.0007 0.0003 0.0005 0.0015 0.0077 0.008 0.003 0.0001 0.0003 0.0081 0.0084 0.0068 0.0016 0.0009 0.0006
Cell Periphery 0.0005 0.0012 0.0008 0.0001 0.0001 0.0002 0.0013 0.0011 0.0005 0.0007 0.0001 0.0002 0.0009 0.0008 0.001 0.0002 0.0004 0.0004
Cytoplasm 0.0062 0.0006 0.0045 0.0002 0.0001 0.0006 0.0004 0.0007 0.0017 0.0002 0.0001 0.0001 0.019 0.0011 0.0023 0.0018 0.0088 0.0002
Cytoplasmic Foci 0.0543 0.0121 0.0294 0.023 0.0033 0.0068 0.0265 0.0183 0.0181 0.003 0.0035 0.0046 0.0267 0.0228 0.0173 0.0276 0.012 0.006
Eisosomes 0.0014 0.0003 0.0004 0.0002 0.0002 0.0004 0.0003 0.0003 0.0002 0.0001 0.0002 0.0002 0.0005 0.0003 0.0006 0.0003 0.0003 0.0003
Endoplasmic Reticulum 0.0039 0.0003 0.0008 0.0001 0.0002 0.002 0.0046 0.0011 0.0004 0.0001 0.0001 0.0003 0.0017 0.0005 0.0011 0.0018 0.004 0.0002
Endosome 0.0689 0.0379 0.037 0.0099 0.0196 0.0109 0.0391 0.045 0.0471 0.0192 0.0144 0.0109 0.0361 0.0519 0.0417 0.0382 0.0537 0.0214
Golgi 0.0687 0.0591 0.0486 0.0653 0.0589 0.0217 0.04 0.0751 0.0674 0.0093 0.1331 0.0396 0.0595 0.1011 0.0821 0.0799 0.0513 0.0957
Lipid Particles 0.053 0.0227 0.036 0.0055 0.0585 0.0234 0.054 0.0477 0.0164 0.0038 0.004 0.0135 0.0725 0.0511 0.0243 0.0439 0.048 0.0128
Mitochondria 0.512 0.7546 0.7087 0.8104 0.6562 0.8084 0.7351 0.6786 0.7616 0.9166 0.8293 0.8849 0.6578 0.6602 0.733 0.6844 0.7048 0.8291
None 0.019 0.0002 0.0004 0.0001 0.0001 0.021 0.0006 0.0002 0.0023 0.0001 0 0.0002 0.0138 0.0003 0.0018 0.0037 0.0056 0.0001
Nuclear Periphery 0.0131 0.0005 0.0025 0 0.0005 0.0108 0.006 0.0028 0.0008 0 0 0.0009 0.0023 0.0013 0.0005 0.0018 0.012 0.0002
Nucleolus 0.0044 0.0135 0.0199 0.0001 0.0001 0.0009 0.0066 0.0101 0.0061 0.0025 0 0.0012 0.0133 0.0069 0.0021 0.001 0.0079 0.0013
Nucleus 0.0071 0.0013 0.002 0 0 0.0006 0.0017 0.0079 0.0013 0.0004 0 0.0004 0.0023 0.0013 0.0008 0.0007 0.0101 0.0002
Peroxisomes 0.0566 0.0188 0.0291 0.0603 0.0023 0.0321 0.0435 0.0397 0.0443 0.0112 0.0077 0.0194 0.0241 0.0196 0.0261 0.0492 0.0273 0.0079
Punctate Nuclear 0.0184 0.0015 0.0011 0.0007 0 0.0009 0.0024 0.0003 0.002 0 0 0.0005 0.0017 0.0002 0.0007 0.0016 0.0015 0
Vacuole 0.0097 0.0247 0.01 0.0005 0.0016 0.0025 0.0102 0.0229 0.0071 0.0169 0.0009 0.0015 0.012 0.0176 0.0075 0.0031 0.0126 0.0019
Vacuole Periphery 0.0129 0.0157 0.0057 0.0016 0.0147 0.0094 0.0135 0.0157 0.0054 0.0056 0.0017 0.0046 0.0127 0.0209 0.0138 0.0034 0.0179 0.0051

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 65.6366 53.9177 61.9751 101.6811 65.9339 79.1931 100.4807 125.5348 132.9393 107.3278
Translational Efficiency 1.6073 1.6046 1.6612 1.0289 1.4008 1.7032 1.3548 1.2745 1.2246 1.1741

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1638 976 2824 1479 1126 183 1732 968 2764 1159 4556 2447

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 821.85 853.57 1099.67 1083.62 717.86 950.25 1073.77 1065.52 779.49 868.84 1089.82 1076.46
Standard Deviation 144.93 154.92 202.64 193.83 123.16 139.54 202.12 216.26 145.73 156.61 202.84 203.19
Intensity Change Log 2 0.054634 0.420124 0.398912 0.404605 0.580911 0.569783 0.228397 0.497322 0.481101

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000949 0.007195 0.015160 0.026195 0.001561 0.003082 0.007744 0.031580 0.001198 0.006545 0.012341 0.028325
Bud Neck 0.002961 0.004081 0.002324 0.005659 0.008933 0.005512 0.007660 0.008101 0.005394 0.004307 0.004353 0.006625
Bud Site 0.007403 0.015805 0.023412 0.076456 0.011692 0.024879 0.033488 0.096728 0.009150 0.017237 0.027242 0.084476
Cell Periphery 0.000421 0.000817 0.000351 0.000983 0.001294 0.000384 0.000400 0.001571 0.000777 0.000749 0.000370 0.001215
Cytoplasm 0.001527 0.002863 0.003777 0.010107 0.004634 0.002579 0.005646 0.012237 0.002793 0.002819 0.004488 0.010950
Cytoplasmic Foci 0.021137 0.035075 0.025623 0.038844 0.039818 0.054576 0.048759 0.040395 0.028747 0.038154 0.034418 0.039458
Eisosomes 0.000054 0.000117 0.000103 0.000159 0.000113 0.000092 0.000094 0.000214 0.000078 0.000113 0.000100 0.000181
Endoplasmic Reticulum 0.000666 0.000428 0.001454 0.001807 0.000944 0.000757 0.000971 0.001171 0.000779 0.000480 0.001270 0.001555
Endosome 0.059850 0.080138 0.039034 0.055766 0.081159 0.114403 0.078632 0.050531 0.068531 0.085548 0.054088 0.053695
Golgi 0.043597 0.069024 0.075346 0.099642 0.084803 0.067543 0.097215 0.088047 0.060383 0.068790 0.083660 0.095055
Lipid Particles 0.005665 0.003738 0.001170 0.002454 0.014953 0.004116 0.004037 0.001880 0.009449 0.003798 0.002260 0.002227
Mitochondria 0.795683* 0.700258* 0.701103* 0.533287* 0.653774* 0.594314* 0.532655* 0.535066* 0.737872* 0.683530* 0.637067* 0.533990*
Mitotic Spindle 0.004145 0.008856 0.043814 0.067768 0.006120 0.028031 0.063966 0.049320 0.004950 0.011884 0.051475 0.060470
None 0.000659 0.000233 0.000577 0.000508 0.000630 0.000239 0.000544 0.000469 0.000647 0.000234 0.000564 0.000493
Nuclear Periphery 0.000398 0.000092 0.000634 0.000367 0.000736 0.000327 0.001230 0.000150 0.000536 0.000130 0.000861 0.000281
Nuclear Periphery Foci 0.000367 0.000234 0.000560 0.000758 0.001015 0.002001 0.001405 0.000286 0.000631 0.000513 0.000881 0.000571
Nucleolus 0.000414 0.000255 0.000133 0.000267 0.001277 0.000456 0.001347 0.000317 0.000766 0.000287 0.000594 0.000287
Nucleus 0.000747 0.000600 0.000625 0.000436 0.001535 0.000634 0.001483 0.000298 0.001068 0.000606 0.000951 0.000382
Peroxisomes 0.025292 0.049300 0.014380 0.031142 0.049084 0.060808 0.027373 0.047011 0.034985 0.051117 0.019320 0.037420
Vacuole 0.011957 0.011861 0.026555 0.027552 0.014164 0.022580 0.047796 0.020935 0.012856 0.013554 0.034630 0.024934
Vacuole Periphery 0.016107 0.009030 0.023863 0.019844 0.021759 0.012687 0.037556 0.013693 0.018410 0.009607 0.029068 0.017411

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.80 -11.02 -11.00 -7.25 -4.20 -1.68 -5.44 -9.16 -8.43 -6.90 -4.84 -12.24 -14.29 -9.96 -7.61
Bud Neck -1.17 0.80 -3.35 -2.03 -6.42 1.38 0.64 0.42 -1.33 -0.36 1.05 1.13 -1.49 -3.11 -4.37
Bud Site -3.54 -9.20 -17.22 -13.95 -13.05 -1.77 -7.60 -13.99 -7.69 -9.92 -3.54 -11.77 -22.03 -17.31 -16.26
Cell Periphery -2.32 1.57 -4.20 -0.78 -4.95 2.52 2.53 -0.65 -4.84 -5.03 0.13 2.77 -2.32 -2.52 -7.04
Cytoplasm -2.07 -5.02 -10.48 -7.12 -7.11 1.60 -1.04 -5.50 -7.65 -5.02 -0.17 -3.23 -10.85 -9.78 -8.64
Cytoplasmic Foci -5.33 -2.51 -7.35 -1.27 -5.73 -1.99 -2.80 -0.13 1.89 2.39 -3.71 -3.37 -5.34 -0.51 -2.63
Eisosomes -9.15 -11.26 -13.98 -4.52 -7.20 1.26 2.17 -6.14 -6.02 -8.25 -5.04 -4.71 -12.86 -7.49 -10.89
Endoplasmic Reticulum 1.28 -3.10 -2.98 -4.00 -0.88 0.57 -0.21 -1.22 -1.27 -1.02 2.25 -2.95 -3.21 -4.82 -1.12
Endosome -3.82 6.13 1.06 5.00 -6.21 -2.68 0.50 6.38 5.34 7.59 -3.57 5.06 4.90 7.21 0.21
Golgi -5.36 -8.45 -11.29 -5.25 -4.81 1.97 -2.10 -0.47 -2.27 1.51 -1.97 -7.12 -8.74 -5.48 -2.92
Lipid Particles 1.36 5.26 3.03 0.97 -2.00 4.33 5.58 6.86 1.29 2.49 4.21 7.62 7.20 1.43 0.09
Mitochondria 7.22 9.80 21.56 11.56 14.53 1.95 8.96 7.57 1.96 -0.15 4.51 12.43 20.94 11.85 11.16
Mitotic Spindle -3.59 -17.76 -16.87 -15.04 -5.63 -3.33 -15.20 -11.35 -2.91 2.73 -4.58 -23.16 -20.28 -15.88 -2.77
None 1.83 0.30 0.65 -4.97 0.61 2.80 0.77 1.40 -3.09 1.51 2.86 0.56 1.14 -6.30 1.03
Nuclear Periphery 2.92 -1.38 0.21 -3.23 1.65 1.37 -1.61 2.39 1.03 5.71 3.37 -2.03 1.98 -2.55 4.72
Nuclear Periphery Foci 1.87 -1.99 -1.81 -2.47 -0.92 -0.56 -1.25 2.60 0.98 6.49 0.39 -1.80 0.28 -0.22 2.01
Nucleolus 1.35 2.16 1.11 -0.14 -1.19 2.20 -0.04 3.03 0.56 2.60 3.11 1.10 3.01 -0.03 1.84
Nucleus 0.27 0.19 0.85 0.48 0.83 1.86 0.19 3.34 0.92 3.41 1.23 0.34 2.68 0.76 2.79
Peroxisomes -5.21 4.48 -1.75 3.88 -6.30 -0.92 4.96 0.42 1.10 -4.41 -3.64 6.83 -0.77 3.11 -7.60
Vacuole 0.04 -9.55 -8.29 -7.50 -0.45 -1.94 -13.23 -3.15 0.29 9.10 -0.50 -15.96 -8.46 -6.46 5.65
Vacuole Periphery 4.61 -4.50 -2.01 -6.65 2.26 3.71 -6.10 3.63 -0.45 10.05 6.78 -7.30 0.71 -6.13 8.13