Standard name
Human Ortholog
Description Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.11 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.15 0.18 0.31 0.14 0.08 0.09 0.08 0.05 0.06 0.05 0.07 0.16 0.14 0.18 0.19 0.24 0.18 0.07 0.08 0.11 0.11 0.1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.37 0.4 0.6 0.37 0.54 0.58 0.55 0.63 0.65 0.65 0.67 0 0 0.12 0.05 0.05 0.05 0 0 0 0 0 0
Nucleus 0.63 0.58 0.51 0.34 0.58 0.67 0.66 0.66 0.69 0.65 0.68 0.7 0.75 0.78 0.76 0.59 0.58 0.61 0.57 0.65 0.5 0.49 0.5 0.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.27 0.16 0.08 0.07 0.12 0.07 0 0 0 0 0 0 0.12 0.08 0.06 0.22 0.18 0.2 0.29 0.18 0.28 0.26 0.19 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 1 0
Bud 0 0 2 1 2 2 2 5 10 9 12 9 1 0 0 0 0 0 10 1 0 8 22 17
Bud Neck 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 4 4
Bud Site 0 0 0 0 2 5 5 12 17 12 11 17 0 0 0 0 0 0
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 2 3 0 0 0 0 0 0
Cytoplasm 50 9 36 68 49 34 48 50 20 33 14 25 22 37 50 37 31 31 36 8 26 11 19 7
Endoplasmic Reticulum 0 0 0 3 0 0 0 1 1 0 0 0 0 2 0 9 2 4 1 0 4 0 0 2
Endosome 17 0 0 0 0 1 1 1 0 0 0 1 2 0 0 4 2 7 4 2 0 0 0 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1 1 0 0 0 0 4
Mitochondria 18 23 81 131 125 218 311 341 277 344 193 245 2 7 35 10 7 8 3 1 5 1 1 3
Nucleus 268 36 104 75 198 270 356 407 306 342 204 258 105 205 213 114 76 102 302 66 115 53 99 121
Nuclear Periphery 0 0 0 0 0 2 0 0 3 1 4 2 0 0 0 1 0 0 2 0 0 0 0 0
Nucleolus 7 1 0 0 0 3 6 10 7 10 3 5 0 1 3 1 0 0 0 0 0 0 2 5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 0 1 0 1 2 1 1 0 1 0 1 2 0 0 0 8 1 4 2 4 7
Vac/Vac Membrane 116 10 16 15 42 30 22 21 13 14 10 13 17 20 18 42 23 34 153 18 65 28 38 60
Unique Cell Count 423 62 202 220 342 404 539 620 441 526 298 366 140 262 281 194 130 168 533 102 234 109 200 244
Labelled Cell Count 477 79 242 293 419 566 752 850 655 766 451 576 149 274 322 221 144 190 533 102 234 109 200 244


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.3 3.8 3.7 4.2 4.0 3.9 4.1 3.9 3.9 3.8 3.8 5.6 5.6 4.7 6.8 7.6 7.7 5.3 6.1 7.2
Std Deviation (1e-4) 0.7 0.6 0.7 0.9 1.0 1.0 1.0 1.1 1.0 1.1 0.8 1.1 1.4 1.3 1.1 1.6 2.1 2.1 1.4 2.0 2.0
Intensity Change (Log2) -0.05 0.13 0.07 0.03 0.09 0.02 0.04 0.0 -0.02 0.55 0.54 0.3 0.82 0.98 0.99 0.47 0.67 0.91


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 3.9 2.7 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8242 1.7768 1.5432 1.2256 1.1846 1.6888 1.9337 2.2326 2.1921 1.8085 2.1246 2.168 -0.1296 1.0557 0.5515 1.2266 0.5875 0.7079
Actin 0.0006 0.0003 0.0007 0.0005 0.0014 0.0002 0.0256 0.0011 0.0122 0.0005 0.008 0.0018 0.0004 0.0018 0.0005 0.0006 0.0017 0.0003
Bud 0.0003 0.0001 0.0002 0.0001 0.0001 0.0002 0.0008 0.0002 0.0004 0.0001 0.0006 0.0002 0.0024 0.0216 0.0001 0.0001 0.0002 0.0002
Bud Neck 0.0012 0.0005 0.0016 0.0016 0.0025 0.007 0.0024 0.0007 0.0026 0.0008 0.0021 0.0098 0.0007 0.001 0.0015 0.0007 0.0045 0.0075
Bud Periphery 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0012 0.0001 0.0005 0.0001 0.0007 0.0003 0.0011 0.0127 0.0001 0.0001 0.0002 0.0002
Bud Site 0.0013 0.0026 0.0018 0.0011 0.0014 0.0003 0.0144 0.0021 0.0081 0.0003 0.0027 0.0004 0.0024 0.0112 0.001 0.0005 0.0014 0.0007
Cell Periphery 0.0003 0.0004 0.0002 0.0001 0.0003 0.0001 0.0006 0.0001 0.0003 0 0.0001 0.0001 0.0003 0.0009 0.0002 0.0002 0.0002 0.0002
Cytoplasm 0.3713 0.3849 0.3012 0.339 0.2173 0.232 0.1176 0.1876 0.11 0.077 0.0807 0.0769 0.3694 0.1936 0.3131 0.2674 0.1227 0.1903
Cytoplasmic Foci 0.0052 0.0022 0.0056 0.0124 0.0153 0.0017 0.0449 0.0018 0.006 0.0113 0.0404 0.004 0.0145 0.0024 0.0041 0.0025 0.0043 0.0049
Eisosomes 0.0001 0.0001 0 0 0.0003 0 0.0002 0 0.0001 0 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0179 0.0081 0.0088 0.0093 0.0095 0.0026 0.0047 0.0045 0.0051 0.0024 0.0051 0.0031 0.0089 0.0068 0.0097 0.0051 0.008 0.0031
Endosome 0.0074 0.002 0.0042 0.014 0.0274 0.0013 0.0287 0.0059 0.0151 0.0128 0.0794 0.0116 0.0043 0.0034 0.0035 0.0017 0.003 0.0022
Golgi 0.0002 0.0001 0.0001 0.0002 0.0006 0 0.0116 0.0003 0.0122 0.0096 0.0342 0.0052 0.0002 0.0008 0.0001 0.0001 0.0002 0.0001
Lipid Particles 0.0011 0.0004 0.0004 0.0009 0.0044 0.0002 0.0259 0.0007 0.009 0.01 0.031 0.002 0.0007 0.0003 0.0007 0.0002 0.0006 0.0006
Mitochondria 0.0006 0.0002 0.0007 0.0005 0.0014 0.0003 0.0146 0.0017 0.0173 0.0222 0.0486 0.0094 0.0005 0.0033 0.0007 0.0006 0.0008 0.0004
None 0.2118 0.1928 0.082 0.1368 0.249 0.074 0.0358 0.0697 0.0347 0.078 0.07 0.0231 0.3252 0.1986 0.2012 0.3637 0.3467 0.2025
Nuclear Periphery 0.0222 0.008 0.0162 0.0207 0.0164 0.0049 0.0306 0.0187 0.0145 0.0271 0.0093 0.0143 0.0149 0.0079 0.0148 0.0107 0.0083 0.0061
Nucleolus 0.0038 0.0017 0.0034 0.0024 0.0125 0.0051 0.0043 0.002 0.0034 0.0027 0.002 0.0052 0.0033 0.0035 0.0018 0.0017 0.0062 0.0029
Nucleus 0.3355 0.3876 0.5597 0.439 0.4011 0.6572 0.5969 0.6901 0.7171 0.7254 0.5607 0.8071 0.2209 0.5179 0.4337 0.3333 0.4711 0.5495
Peroxisomes 0.0001 0 0.0003 0.0001 0.0007 0 0.0141 0.0002 0.0099 0.0061 0.0097 0.0005 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001
Punctate Nuclear 0.0117 0.0048 0.0094 0.0144 0.0315 0.0111 0.017 0.008 0.0148 0.0116 0.0106 0.0223 0.0225 0.0058 0.0102 0.0089 0.0161 0.0259
Vacuole 0.0066 0.003 0.003 0.0064 0.0062 0.0016 0.0062 0.0027 0.006 0.0012 0.0027 0.0021 0.0069 0.0057 0.0026 0.0016 0.0029 0.0022
Vacuole Periphery 0.0005 0.0001 0.0003 0.0004 0.0006 0.0001 0.0019 0.0016 0.0008 0.0006 0.0012 0.0007 0.0004 0.0006 0.0003 0.0002 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.976 30.6761 31.2474 50.3643 33.571 24.6312 39.4052 41.9634 43.9581 55.0303
Translational Efficiency 0.6131 0.5252 0.7765 0.4831 0.5315 0.6805 0.6275 0.4967 0.5614 0.499

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1359 25 286 1200 1613 2223 193 347 2972 2248 479 1547

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 715.85 321.26 1108.74 935.95 950.87 745.64 1038.19 1176.85 843.40 740.92 1080.31 989.99
Standard Deviation 251.12 8.58 129.40 118.39 152.26 98.49 109.57 203.12 234.79 107.58 126.62 173.85
Intensity Change Log 2 -1.155916 0.631192 0.386774 -0.350769 0.126750 0.307610 -0.643587 0.365263 0.342144

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000677 0.000036 0.000740 0.003249 0.000737 0.000314 0.000869 0.001535 0.000710 0.000311 0.000792 0.002865
Bud Neck 0.003645 0.000023 0.002729 0.029553 0.007437 0.012651 0.003219 0.035972 0.005703 0.012511 0.002926 0.030993
Bud Site 0.004477 0.000039 0.001624 0.036214 0.006311 0.005833 0.001286 0.013129 0.005472 0.005768 0.001488 0.031036
Cell Periphery 0.000532 0.000123 0.000124 0.000275 0.000905 0.000128 0.000106 0.000125 0.000735 0.000128 0.000116 0.000242
Cytoplasm 0.091819 0.000045 0.088989 0.125859* 0.198688* 0.276514* 0.017478 0.157882* 0.149820* 0.273440* 0.060176 0.133042*
Cytoplasmic Foci 0.003101 0.000014 0.001283 0.008718 0.005946 0.046451 0.000324 0.000748 0.004645 0.045934 0.000896 0.006931
Eisosomes 0.000023 0.000100 0.000023 0.000050 0.000063 0.000044 0.000049 0.000013 0.000045 0.000045 0.000034 0.000042
Endoplasmic Reticulum 0.005187 0.000001 0.007042 0.009149 0.010374 0.001254 0.012743 0.000903 0.008002 0.001240 0.009339 0.007300
Endosome 0.000472 0.000001 0.000843 0.009267 0.000733 0.003233 0.000648 0.000201 0.000614 0.003197 0.000765 0.007234
Golgi 0.000343 0.000005 0.000088 0.005347 0.000245 0.001619 0.000056 0.000037 0.000290 0.001601 0.000075 0.004156
Lipid Particles 0.000468 0.000024 0.000544 0.001031 0.000723 0.003831 0.000585 0.000134 0.000606 0.003789 0.000561 0.000830
Mitochondria 0.001462 0.000012 0.000611 0.008779 0.001214 0.005878 0.000842 0.004265 0.001327 0.005813 0.000704 0.007767
Mitotic Spindle 0.001101 0.000279 0.000207 0.051898 0.001107 0.008127 0.000418 0.006326 0.001105 0.008039 0.000292 0.041676
None 0.042501 0.999130* 0.002805 0.005642 0.006928 0.005639 0.002373 0.005841 0.023195 0.016688 0.002631 0.005687
Nuclear Periphery 0.000344 0.000000 0.000906 0.003054 0.000798 0.001189 0.001236 0.001205 0.000591 0.001176 0.001039 0.002640
Nuclear Periphery Foci 0.000942 0.000034 0.001322 0.001456 0.002881 0.000713 0.000440 0.000026 0.001995 0.000705 0.000966 0.001136
Nucleolus 0.005489 0.000033 0.000371 0.001735 0.000624 0.002547 0.000996 0.000312 0.002849 0.002519 0.000623 0.001415
Nucleus 0.834886* 0.000013 0.865340* 0.641417* 0.741631* 0.605314* 0.944179* 0.761938* 0.784273* 0.598582* 0.897106* 0.668451*
Peroxisomes 0.000606 0.000087 0.000099 0.002637 0.000167 0.004202 0.000164 0.001207 0.000368 0.004156 0.000125 0.002316
Vacuole 0.001619 0.000001 0.024202 0.052320 0.012278 0.013764 0.011888 0.008107 0.007404 0.013611 0.019240 0.042403
Vacuole Periphery 0.000304 0.000000 0.000109 0.002347 0.000209 0.000754 0.000101 0.000094 0.000253 0.000746 0.000106 0.001842

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 6.05 -2.06 -2.17 -3.60 -1.56 3.40 -2.84 -0.03 -0.76 0.65 3.37 -3.27 -1.94 -2.97 -1.07
Bud Neck 7.03 -0.53 -10.05 -13.13 -9.66 -4.12 3.55 -4.72 -3.56 -5.36 -5.89 2.72 -9.96 -4.80 -10.82
Bud Site 5.53 2.40 -5.15 -7.58 -6.40 0.89 4.62 -0.89 -1.19 -2.39 -0.09 5.46 -4.91 -4.63 -6.77
Cell Periphery 3.97 4.99 4.32 -1.17 -2.60 9.72 9.34 10.02 2.14 2.58 10.97 10.43 9.92 -4.10 -1.89
Cytoplasm 19.05 1.07 0.09 -22.81 -1.05 -10.55 24.36 5.47 13.46 -12.35 -19.26 13.85 8.51 26.69 -7.09
Cytoplasmic Foci 5.47 2.76 0.29 -5.53 -2.57 -18.51 8.40 7.35 21.19 -0.61 -19.30 7.78 3.51 20.24 -3.00
Eisosomes -1.42 -2.32 -15.57 0.45 -9.24 2.23 -0.48 5.13 11.49 7.07 0.33 -0.30 -2.20 -5.11 -2.43
Endoplasmic Reticulum 12.22 -3.90 -11.97 -24.10 -5.52 13.85 -3.93 13.19 -0.74 11.42 16.22 -4.23 -4.01 -20.64 1.21
Endosome 6.91 -3.63 -5.23 -6.16 -3.75 -6.94 -1.66 7.21 8.39 3.15 -7.34 -3.39 -4.73 1.17 -3.05
Golgi 4.32 2.88 -1.41 -2.04 -1.84 -3.48 2.81 3.60 4.01 2.75 -3.34 3.91 -1.40 1.10 -1.83
Lipid Particles 7.97 -2.37 -10.25 -19.79 -4.78 -7.67 -1.20 7.74 9.13 4.31 -8.03 -2.14 -7.09 6.73 -2.43
Mitochondria 3.48 1.67 -2.61 -5.10 -4.02 -5.37 0.44 -2.29 2.10 -2.58 -5.39 1.57 -3.49 2.29 -4.56
Mitotic Spindle 1.33 -0.51 -7.69 -8.07 -7.49 -4.88 0.10 -2.26 -0.01 -2.26 -4.89 -0.45 -8.06 -4.92 -7.89
None -305.38* 12.85 11.96 2299.13* -5.75 1.08 5.16 -0.95 -2.19 -6.46 2.38 13.54 11.27 4.78 -8.04
Nuclear Periphery 8.17 -9.00 -20.56 -22.30 -15.26 -4.25 -4.68 -5.34 -4.06 -2.93 -6.73 -9.13 -20.24 -16.21 -15.04
Nuclear Periphery Foci 6.98 -2.26 -3.67 -8.50 0.30 4.12 3.70 5.84 2.94 3.12 3.68 1.11 1.72 -2.62 0.30
Nucleolus 10.50 9.60 7.75 -7.04 -4.25 -7.01 -2.33 -0.52 6.48 1.54 0.35 8.52 6.08 5.06 -3.04
Nucleus 119.09* -1.03 13.44 -83.24* 11.16 15.48 -14.72 -2.54 -12.49 8.82 23.47 -9.74 8.88 -11.75 15.52
Peroxisomes 2.86 2.68 -0.30 -2.46 -2.32 -7.72 -1.26 -0.89 5.32 -0.74 -7.25 2.33 -1.29 6.25 -2.30
Vacuole 9.62 -7.52 -21.17 -21.51 -9.45 -0.25 -2.51 -1.18 -1.09 1.57 -5.64 -7.08 -19.32 -17.67 -9.55
Vacuole Periphery 3.14 1.80 -1.64 -3.56 -2.84 -2.88 0.80 1.42 3.92 2.12 -2.74 1.85 -1.73 1.03 -2.74