Standard name
Description Nitric oxide oxidoreductase; flavohemoglobin that plays role in oxidative and nitrosative stress responses; protects against nitration of cellular targets and against cell growth inhibition under aerobic or anaerobic conditions; yeast flavohemoglobin Yhb1p and human homolog neuroglobin NGB protect cells against alpha-synuclein cytotoxicity and aggregate formation; protein increases in abundance, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0.09 0.14 0.13
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 1.0 0.99 0.95 0.91 0.83 0.68 0.56 0.65 0.57 0.58 0.6 0.97 0.96 0.9 0.93 0.96 0.98 0.88 0.88 0.9 0.83 0.8 0.8
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.15 0.17 0.15 0.2 0.14 0.2 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.06 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0.07 0.11 0.16 0.12 0.09 0.1 0.08 0 0 0.06 0.09 0.05 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 7 15 13 13 26 26 18 0 2 6 0 0 0 0 0 0 0 0 3
Bud 0 1 0 0 3 1 2 5 2 8 6 5 0 4 3 0 0 0 11 14 10 14 61 62
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 1 3 2 7 6 11 16 17 20 13 10 1 6 7 2 5 1 0 0 0 0 0 0
Cytoplasm 152 201 277 260 466 363 418 319 283 294 225 252 347 492 596 86 110 40 143 187 243 134 364 382
Endoplasmic Reticulum 0 0 1 2 10 20 94 99 64 104 53 84 2 2 13 2 6 2 1 0 3 2 1 1
Endosome 0 0 0 0 0 4 1 0 1 0 0 0 0 0 3 0 0 1 0 1 1 1 0 0
Golgi 0 0 0 2 1 9 19 37 18 27 31 17 2 0 8 0 0 0 0 0 0 0 0 0
Mitochondria 5 0 2 2 4 12 20 9 8 24 18 11 0 0 0 0 0 0 0 0 1 0 1 2
Nucleus 0 0 0 1 1 0 1 3 2 3 2 1 2 2 0 2 0 0 1 3 0 3 8 5
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 2 0 0 1 1 3 4 11 5 13 8 12 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 3 5 0 0 0 1 0 2 1 2 2
Vac/Vac Membrane 2 0 5 9 30 32 65 90 50 49 37 34 6 19 40 8 6 2 0 3 2 2 9 4
Unique Cell Count 159 201 281 275 514 435 612 571 433 519 386 422 357 512 660 92 115 41 163 214 270 162 456 476
Labelled Cell Count 161 203 288 279 523 457 650 603 463 568 419 444 360 531 682 100 127 46 163 214 270 162 456 476


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.4 23.7 14.6 20.5 25.8 26.0 30.4 34.6 37.0 39.0 39.8 44.0 25.8 29.8 30.1 20.5 27.3 36.0 21.8 23.5 23.7
Std Deviation (1e-4) 6.0 13.2 7.2 9.3 12.6 11.8 12.0 12.5 13.0 13.3 15.3 14.1 15.7 17.8 18.6 11.4 17.4 20.9 13.7 13.3 12.9
Intensity Change (Log2) – – – 0.5 0.83 0.84 1.06 1.25 1.35 1.42 1.45 1.6 0.83 1.03 1.05 0.49 0.91 1.31 0.58 0.69 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 2.9 3.8 4.4 3.5 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1.6 2.3 2.3 1.9 1.3 0 0 0 0 0 0
Cytoplasm -2.6 -4.3 -6.4 -10.1 -12.8 -10.5 -12.5 -11.9 -11.7 -1.2 -2.0 -4.5 -2.6 -1.8 -0.5
Endoplasmic Reticulum 0 0 3.3 6.8 7.2 6.5 7.9 6.3 7.8 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 3.0 4.4 3.5 3.9 4.9 3.4 0 0 0 0 0 0
Mitochondria 0 0 1.9 2.3 0 0 3.0 3.0 1.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.1 2.7 3.3 4.6 6.1 4.8 4.1 4.1 3.6 0 1.5 2.8 0 0 0