Standard name | |
---|---|
Human Ortholog | |
Description | Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 149 | 104 | 82 | 109 | 108 | 110 | 129 | 127 | 105 | 138 | 121 | 146 | 89 | 112 | 105 | 163 | 140 | 127 | 78 | 68 | 42 | 2 | 12 | 18 |
Bud | 62 | 51 | 36 | 47 | 71 | 51 | 61 | 68 | 63 | 53 | 73 | 69 | 23 | 44 | 39 | 74 | 57 | 68 | 96 | 87 | 71 | 24 | 68 | 74 |
Bud Neck | 3 | 1 | 3 | 3 | 2 | 2 | 3 | 6 | 4 | 5 | 4 | 4 | 0 | 2 | 0 | 4 | 16 | 5 | 57 | 49 | 43 | 14 | 49 | 34 |
Bud Site | 69 | 20 | 16 | 28 | 25 | 37 | 28 | 23 | 32 | 29 | 19 | 32 | 26 | 20 | 2 | 23 | 27 | 27 | – | – | – | – | – | – |
Cell Periphery | 33 | 27 | 22 | 43 | 12 | 24 | 37 | 27 | 46 | 31 | 29 | 53 | 33 | 57 | 99 | 44 | 39 | 25 | 29 | 9 | 16 | 0 | 0 | 0 |
Cytoplasm | 16 | 27 | 22 | 30 | 42 | 44 | 53 | 67 | 40 | 31 | 33 | 45 | 35 | 32 | 42 | 59 | 53 | 47 | 0 | 4 | 3 | 4 | 8 | 19 |
Endoplasmic Reticulum | 9 | 8 | 9 | 14 | 9 | 15 | 34 | 28 | 19 | 29 | 27 | 16 | 25 | 18 | 28 | 8 | 13 | 8 | 3 | 2 | 1 | 0 | 1 | 0 |
Endosome | 1 | 3 | 0 | 3 | 3 | 1 | 3 | 0 | 3 | 3 | 1 | 5 | 1 | 3 | 3 | 4 | 5 | 5 | 1 | 0 | 1 | 0 | 2 | 0 |
Golgi | 2 | 2 | 1 | 1 | 0 | 0 | 1 | 3 | 0 | 1 | 0 | 1 | 1 | 1 | 4 | 48 | 25 | 30 | 3 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 44 | 7 | 1 | 3 | 0 | 24 | 21 | 31 | 84 | 64 | 170 | 105 | 2 | 1 | 3 | 41 | 26 | 32 | 4 | 3 | 5 | 0 | 0 | 0 |
Nucleus | 2 | 7 | 7 | 4 | 8 | 12 | 20 | 12 | 7 | 7 | 9 | 15 | 8 | 7 | 3 | 23 | 8 | 10 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 6 | 3 | 0 | 4 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4 | 3 | 0 | 2 | 7 | 6 | 8 | 12 | 9 | 9 | 11 | 24 | 0 | 4 | 0 | 11 | 6 | 14 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 15 | 4 | 3 | 7 | 2 | 8 | 13 | 11 | 19 | 22 | 17 | 30 | 8 | 13 | 29 | 13 | 25 | 21 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 7 | 6 | 13 | 5 | 17 | 11 | 10 | 20 | 10 | 13 | 4 | 15 | 15 | 32 | 26 | 35 | 37 | 38 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 9 | 6 | 3 | 3 | 10 | 9 | 10 | 17 | 16 | 8 | 15 | 7 | 7 | 5 | 20 | 9 | 11 | 1 | 4 | 2 | 0 | 0 | 1 |
Unique Cell Count | 234 | 178 | 142 | 191 | 206 | 228 | 278 | 294 | 291 | 284 | 299 | 364 | 186 | 229 | 260 | 366 | 308 | 299 | 282 | 238 | 191 | 51 | 149 | 156 |
Labelled Cell Count | 419 | 279 | 221 | 302 | 309 | 356 | 431 | 445 | 458 | 453 | 532 | 578 | 273 | 357 | 389 | 571 | 486 | 468 | 282 | 238 | 191 | 51 | 149 | 156 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.3 | 10.6 | 9.5 | 9.1 | 9.2 | 8.3 | 8.1 | 8.1 | 7.3 | 7.3 | 6.6 | 7.1 | 9.9 | 10.0 | 10.2 | 12.6 | 12.2 | 11.6 | 10.6 | 9.7 | 10.2 |
Std Deviation (1e-4) | 1.6 | 2.6 | 2.0 | 1.5 | 2.2 | 2.2 | 1.9 | 1.8 | 1.7 | 1.6 | 1.4 | 1.5 | 2.2 | 2.4 | 2.3 | 4.2 | 4.2 | 3.8 | 2.1 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.06 | -0.2 | -0.24 | -0.23 | -0.38 | -0.38 | -0.53 | -0.43 | 0.05 | 0.07 | 0.1 | 0.4 | 0.36 | 0.29 | 0.15 | 0.03 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.1 | -1.0 | -1.8 | -2.2 | -2.8 | -4.3 | -1.8 | -3.4 | -3.6 | -1.8 | -1.7 | -3.3 | -2.7 | -2.4 | -3.0 |
Bud | -0.2 | 1.8 | -0.7 | -0.8 | -0.5 | -0.9 | -1.6 | -0.2 | -1.6 | -3.0 | -1.4 | -2.5 | -1.3 | -1.7 | -0.6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 |
Bud Site | 0.9 | 0.2 | 1.3 | -0.4 | -1.2 | -0.1 | -0.3 | -1.8 | -0.9 | 0.7 | -0.8 | -4.9 | -1.9 | -0.8 | -0.7 |
Cell Periphery | 1.6 | -3.0 | -1.4 | -0.6 | -2.0 | 0.1 | -1.3 | -1.8 | -0.3 | 0.5 | 2.1 | 4.7 | -1.0 | -0.8 | -2.3 |
Cytoplasm | 0.1 | 1.2 | 0.9 | 0.9 | 1.8 | -0.5 | -1.3 | -1.3 | -0.9 | 0.8 | -0.4 | 0.2 | 0.2 | 0.5 | 0.1 |
Endoplasmic Reticulum | 0.4 | -0.8 | 0.1 | 1.9 | 1.1 | 0.1 | 1.3 | 1.0 | -0.9 | 2.1 | 0.5 | 1.5 | 0 | -1.0 | -1.9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 3.1 | 3.6 |
Mitochondria | 0 | 0 | 3.7 | 3.0 | 3.7 | 6.9 | 5.9 | 11.3 | 7.0 | 0 | 0 | 0 | 3.9 | 3.2 | 3.7 |
Nucleus | 0 | -0.5 | 0.1 | 0.9 | -0.4 | 0 | 0 | -1.0 | -0.4 | -0.3 | -0.9 | 0 | 0.6 | 0 | -0.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1.3 | 0 | 2.0 | 2.3 | 1.7 | 2.5 | 0 | 1.6 | 3.2 | 0 | 2.5 | 2.1 |
SpindlePole | -2.6 | -0.3 | -1.6 | -2.4 | -0.9 | -2.5 | -1.9 | -4.0 | -2.2 | -0.4 | 1.4 | 0.3 | 0.1 | 0.9 | 1.1 |
Vacuole | 0 | 0 | 0.1 | -0.5 | -0.4 | 0.7 | 0.6 | 0 | -0.1 | -0.2 | 0 | 0 | 0.6 | 0 | -0.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.5826 | 5.0796 | 5.2029 | 5.5727 | 4.9884 | 5.6679 | 4.5366 | 4.5413 | 4.6073 | 3.3403 | 4.3862 | 5.4315 | 4.7128 | 5.2291 | 4.6257 | 5.2348 | 3.1636 | 5.553 |
Actin | 0.2117 | 0.1099 | 0.1177 | 0.1065 | 0.4105 | 0.1765 | 0.214 | 0.0748 | 0.164 | 0.2209 | 0.0932 | 0.1664 | 0.2602 | 0.1223 | 0.078 | 0.2548 | 0.1016 | 0.2256 |
Bud | 0.04 | 0.0741 | 0.25 | 0.1069 | 0.055 | 0.0155 | 0.0237 | 0.0464 | 0.2137 | 0.0572 | 0.0441 | 0.0069 | 0.0358 | 0.0233 | 0.3564 | 0.0879 | 0.0424 | 0.0077 |
Bud Neck | 0.0655 | 0.0075 | 0.0111 | 0.0405 | 0.0354 | 0.6015 | 0.0781 | 0.0203 | 0.0172 | 0.0056 | 0.1496 | 0.6823 | 0.0737 | 0.0353 | 0.0105 | 0.0052 | 0.0013 | 0.6416 |
Bud Periphery | 0.1179 | 0.0968 | 0.4612 | 0.6177 | 0.3534 | 0.0868 | 0.0863 | 0.0698 | 0.3928 | 0.4114 | 0.2825 | 0.0345 | 0.1031 | 0.0344 | 0.4254 | 0.4952 | 0.2837 | 0.0398 |
Bud Site | 0.3353 | 0.5922 | 0.1207 | 0.0325 | 0.0357 | 0.0313 | 0.3173 | 0.7192 | 0.1329 | 0.0118 | 0.0664 | 0.0204 | 0.2393 | 0.6547 | 0.0791 | 0.0227 | 0.0042 | 0.0134 |
Cell Periphery | 0.121 | 0.0152 | 0.017 | 0.0291 | 0.0326 | 0.057 | 0.0894 | 0.0205 | 0.0167 | 0.0263 | 0.0456 | 0.0377 | 0.0589 | 0.0173 | 0.0088 | 0.0172 | 0.014 | 0.0219 |
Cytoplasm | 0.0371 | 0.0639 | 0.0114 | 0.0314 | 0.0521 | 0.0091 | 0.0277 | 0.0301 | 0.0146 | 0.0077 | 0.0463 | 0.0129 | 0.0463 | 0.0723 | 0.0084 | 0.0359 | 0.0126 | 0.0073 |
Cytoplasmic Foci | 0.0041 | 0.0133 | 0.0005 | 0.0003 | 0.0008 | 0.0022 | 0.027 | 0.0015 | 0.0051 | 0.0303 | 0.0224 | 0.0034 | 0.0245 | 0.0028 | 0.0035 | 0.0111 | 0.0108 | 0.0027 |
Eisosomes | 0.0316 | 0.0007 | 0.0005 | 0.0273 | 0.0028 | 0.0059 | 0.0096 | 0.0004 | 0.0007 | 0.0031 | 0.001 | 0.0022 | 0.0112 | 0.0037 | 0.0147 | 0.002 | 0.0007 | 0.002 |
Endoplasmic Reticulum | 0.0094 | 0.0009 | 0.0007 | 0.0019 | 0.0016 | 0.0011 | 0.0117 | 0.0006 | 0.0011 | 0.004 | 0.0073 | 0.0016 | 0.0082 | 0.0025 | 0.0005 | 0.002 | 0.0025 | 0.0004 |
Endosome | 0.004 | 0.0006 | 0.0002 | 0.0003 | 0.0009 | 0.0009 | 0.0231 | 0.0018 | 0.0099 | 0.1043 | 0.0487 | 0.0049 | 0.0347 | 0.0059 | 0.0016 | 0.016 | 0.1342 | 0.0103 |
Golgi | 0.0035 | 0.0014 | 0.0004 | 0.0008 | 0.0022 | 0.0021 | 0.009 | 0.001 | 0.0099 | 0.0534 | 0.0141 | 0.0023 | 0.0279 | 0.0029 | 0.0045 | 0.0392 | 0.0708 | 0.0071 |
Lipid Particles | 0.002 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0008 | 0.0155 | 0.0015 | 0.0039 | 0.0142 | 0.0576 | 0.0012 | 0.0139 | 0.0015 | 0.0021 | 0.0018 | 0.0134 | 0.0057 |
Mitochondria | 0.0032 | 0.0023 | 0.001 | 0.001 | 0.01 | 0.0014 | 0.0102 | 0.0006 | 0.0086 | 0.0267 | 0.0415 | 0.0008 | 0.0258 | 0.0035 | 0.0024 | 0.0045 | 0.1641 | 0.0082 |
None | 0.0018 | 0.0019 | 0.0025 | 0.0005 | 0.0009 | 0.0004 | 0.0042 | 0.0037 | 0.0006 | 0.003 | 0.0024 | 0.0031 | 0.0034 | 0.0006 | 0.0007 | 0.0005 | 0.0066 | 0.0001 |
Nuclear Periphery | 0.0018 | 0.0007 | 0.0004 | 0.0006 | 0.0006 | 0.0004 | 0.0103 | 0.0005 | 0.0006 | 0.0013 | 0.0137 | 0.0015 | 0.0123 | 0.0115 | 0.0002 | 0.0006 | 0.0125 | 0.0002 |
Nucleolus | 0.0008 | 0.0007 | 0.0002 | 0.0001 | 0.0006 | 0.0011 | 0.0016 | 0.0006 | 0.0002 | 0.0007 | 0.0027 | 0.0007 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0046 | 0.0002 |
Nucleus | 0.0013 | 0.0014 | 0.0007 | 0.0004 | 0.001 | 0.0011 | 0.0096 | 0.0014 | 0.0006 | 0.0008 | 0.0063 | 0.0031 | 0.0057 | 0.0014 | 0.0003 | 0.0003 | 0.088 | 0.0004 |
Peroxisomes | 0.0009 | 0.0048 | 0.0002 | 0.0001 | 0.001 | 0.0012 | 0.0109 | 0.0006 | 0.0039 | 0.0073 | 0.0361 | 0.0008 | 0.0062 | 0.0006 | 0.0023 | 0.0016 | 0.0029 | 0.0012 |
Punctate Nuclear | 0.0006 | 0.0086 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0104 | 0.0004 | 0.0003 | 0.0012 | 0.0033 | 0.0055 | 0.002 | 0.0003 | 0.0002 | 0.0002 | 0.0072 | 0.0002 |
Vacuole | 0.0058 | 0.0024 | 0.0026 | 0.0016 | 0.0022 | 0.003 | 0.0079 | 0.0042 | 0.0021 | 0.0065 | 0.0105 | 0.0076 | 0.0046 | 0.0011 | 0.0004 | 0.0009 | 0.013 | 0.0032 |
Vacuole Periphery | 0.0006 | 0.0003 | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0.0023 | 0.0003 | 0.0005 | 0.0024 | 0.0047 | 0.0003 | 0.0014 | 0.0018 | 0.0001 | 0.0003 | 0.009 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.7152 | 20.2245 | 17.1513 | 26.8879 | 18.8569 | 18.0892 | 22.786 | 27.209 | 22.951 | 25.2589 |
Translational Efficiency | 1.1519 | 1.1454 | 1.2381 | 0.6249 | 1.0127 | 1.3208 | 0.9478 | 0.8156 | 1.0543 | 0.8936 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2179 | 339 | 185 | 283 | 1086 | 1729 | 160 | 165 | 3265 | 2068 | 345 | 448 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 822.46 | 950.56 | 1401.98 | 1349.57 | 882.73 | 983.14 | 1302.66 | 1318.34 | 842.51 | 977.80 | 1355.92 | 1338.07 |
Standard Deviation | 107.24 | 188.94 | 176.66 | 250.98 | 135.36 | 149.64 | 198.73 | 215.90 | 120.73 | 157.23 | 193.66 | 239.14 |
Intensity Change Log 2 | — | 0.208832 | 0.769448 | 0.714482 | — | 0.155425 | 0.561416 | 0.578678 | — | 0.181431 | 0.665504 | 0.645777 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.079448 | 0.083856 | 0.239938* | 0.088924 | 0.104587* | 0.076205 | 0.138846* | 0.051884 | 0.087810 | 0.077459 | 0.193055* | 0.075282 |
Bud Neck | 0.170298 | 0.096099 | 0.133372 | 0.050845 | 0.113495 | 0.137951 | 0.068401 | 0.052668 | 0.151404 | 0.131091 | 0.103240 | 0.051516 |
Bud Site | 0.518776* | 0.628734* | 0.455373* | 0.508162* | 0.518854* | 0.611728* | 0.598881* | 0.515323* | 0.518802* | 0.614516* | 0.521928* | 0.510800* |
Cell Periphery | 0.146731* | 0.037625 | 0.048447 | 0.275884* | 0.138289* | 0.102504* | 0.050397 | 0.312635* | 0.143923* | 0.091868 | 0.049351 | 0.289419* |
Cytoplasm | 0.044790 | 0.053411 | 0.011536 | 0.008040 | 0.041231 | 0.027005 | 0.018804 | 0.004994 | 0.043607 | 0.031334 | 0.014907 | 0.006918 |
Cytoplasmic Foci | 0.006576 | 0.026078 | 0.008578 | 0.001584 | 0.017919 | 0.005326 | 0.010223 | 0.001256 | 0.010349 | 0.008728 | 0.009341 | 0.001463 |
Eisosomes | 0.005601 | 0.001356 | 0.013766 | 0.047181 | 0.005843 | 0.002508 | 0.012839 | 0.035815 | 0.005681 | 0.002319 | 0.013336 | 0.042995 |
Endoplasmic Reticulum | 0.001778 | 0.003794 | 0.023800 | 0.005726 | 0.004463 | 0.001876 | 0.007384 | 0.005638 | 0.002671 | 0.002190 | 0.016187 | 0.005693 |
Endosome | 0.002810 | 0.018497 | 0.004382 | 0.001154 | 0.009238 | 0.004811 | 0.003687 | 0.001479 | 0.004948 | 0.007055 | 0.004060 | 0.001274 |
Golgi | 0.001792 | 0.007375 | 0.001491 | 0.000338 | 0.009607 | 0.003340 | 0.000773 | 0.001734 | 0.004392 | 0.004001 | 0.001158 | 0.000852 |
Lipid Particles | 0.001003 | 0.002381 | 0.006159 | 0.000514 | 0.003503 | 0.000311 | 0.003117 | 0.000294 | 0.001835 | 0.000650 | 0.004748 | 0.000433 |
Mitochondria | 0.007904 | 0.007545 | 0.001806 | 0.001836 | 0.011957 | 0.012612 | 0.002653 | 0.003399 | 0.009252 | 0.011781 | 0.002198 | 0.002412 |
Mitotic Spindle | 0.000429 | 0.002257 | 0.003595 | 0.000498 | 0.000491 | 0.001652 | 0.004724 | 0.000586 | 0.000450 | 0.001752 | 0.004118 | 0.000531 |
None | 0.000492 | 0.000477 | 0.000029 | 0.000050 | 0.000263 | 0.000242 | 0.000031 | 0.000043 | 0.000416 | 0.000280 | 0.000030 | 0.000047 |
Nuclear Periphery | 0.000009 | 0.000019 | 0.000085 | 0.000080 | 0.000019 | 0.000025 | 0.000096 | 0.000061 | 0.000013 | 0.000024 | 0.000090 | 0.000073 |
Nuclear Periphery Foci | 0.000113 | 0.000270 | 0.001917 | 0.000113 | 0.000450 | 0.000180 | 0.001347 | 0.000095 | 0.000225 | 0.000195 | 0.001653 | 0.000107 |
Nucleolus | 0.000059 | 0.000124 | 0.000068 | 0.000012 | 0.000582 | 0.000651 | 0.000031 | 0.000020 | 0.000233 | 0.000564 | 0.000051 | 0.000015 |
Nucleus | 0.002181 | 0.001233 | 0.003103 | 0.000403 | 0.002402 | 0.001623 | 0.004096 | 0.000380 | 0.002254 | 0.001559 | 0.003563 | 0.000395 |
Peroxisomes | 0.005317 | 0.010772 | 0.004323 | 0.000253 | 0.009172 | 0.002906 | 0.002065 | 0.002040 | 0.006599 | 0.004195 | 0.003276 | 0.000911 |
Vacuole | 0.003595 | 0.015913 | 0.038017 | 0.006821 | 0.006029 | 0.004978 | 0.071435 | 0.009383 | 0.004405 | 0.006771 | 0.053515 | 0.007765 |
Vacuole Periphery | 0.000297 | 0.002183 | 0.000215 | 0.001582 | 0.001605 | 0.001566 | 0.000171 | 0.000270 | 0.000732 | 0.001667 | 0.000195 | 0.001099 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.38 | -6.61 | -0.74 | -0.33 | 5.51 | 3.88 | -0.98 | 4.32 | 2.13 | 3.31 | 2.06 | -5.92 | 1.40 | 0.24 | 6.02 |
Bud Neck | 6.83 | 2.77 | 15.18 | 4.05 | 4.46 | -2.91 | 4.51 | 5.71 | 8.42 | 0.67 | 3.25 | 5.35 | 15.69 | 11.70 | 4.09 |
Bud Site | -5.52 | 3.25 | 0.44 | 4.05 | -2.19 | -6.80 | -0.85 | 0.08 | 3.06 | 0.70 | -10.01 | 1.61 | 0.39 | 5.22 | -0.95 |
Cell Periphery | 14.08 | 11.05 | -5.95 | -11.03 | -10.40 | 4.05 | 7.07 | -5.98 | -7.37 | -8.88 | 8.88 | 12.77 | -8.37 | -11.44 | -13.61 |
Cytoplasm | -1.33 | 5.06 | 10.21 | 5.88 | 1.56 | 3.23 | 2.13 | 8.80 | 7.35 | 4.05 | 3.70 | 5.21 | 13.46 | 8.61 | 3.74 |
Cytoplasmic Foci | -4.52 | -1.38 | 4.31 | 5.52 | 2.90 | 6.09 | 1.42 | 8.35 | 4.35 | 3.73 | 1.21 | -0.58 | 8.98 | 6.35 | 4.60 |
Eisosomes | 8.33 | -2.61 | -6.02 | -6.65 | -4.64 | 5.59 | -1.41 | -4.89 | -5.46 | -3.36 | 8.26 | -2.75 | -7.60 | -8.30 | -5.68 |
Endoplasmic Reticulum | -1.27 | -3.74 | -1.77 | -0.70 | 2.87 | 4.06 | -1.13 | -0.63 | -2.11 | 0.62 | 1.06 | -3.97 | -1.93 | -2.21 | 2.82 |
Endosome | -4.49 | -2.67 | 2.90 | 4.96 | 4.36 | 3.58 | 3.24 | 6.44 | 4.00 | 3.39 | -2.32 | -0.35 | 6.65 | 6.89 | 5.61 |
Golgi | -3.71 | 0.89 | 4.93 | 4.75 | 3.34 | 4.36 | 6.89 | 4.47 | 1.15 | -0.81 | 0.53 | 6.55 | 5.44 | 4.11 | 0.50 |
Lipid Particles | -1.63 | -2.20 | 1.00 | 2.30 | 2.38 | 4.20 | -0.31 | 4.20 | 0.17 | 2.77 | 3.03 | -2.34 | 3.52 | 0.96 | 3.23 |
Mitochondria | 0.14 | 6.64 | 7.85 | 2.09 | -0.19 | -0.28 | 5.14 | 4.29 | 5.19 | -0.79 | -1.68 | 8.25 | 8.01 | 6.89 | -0.60 |
Mitotic Spindle | -1.81 | -1.26 | -1.11 | 1.69 | 1.22 | -3.02 | -1.52 | -0.67 | 2.72 | 1.48 | -3.61 | -1.95 | -1.31 | 3.28 | 1.90 |
None | 0.02 | 3.73 | 3.60 | 1.36 | -1.51 | 0.03 | 3.05 | 3.05 | 3.31 | -0.06 | 0.98 | 4.45 | 4.34 | 3.32 | -1.28 |
Nuclear Periphery | -2.80 | -4.53 | -4.02 | -3.36 | 1.76 | -1.01 | -4.20 | -1.66 | -1.32 | 2.59 | -2.20 | -6.22 | -4.21 | -3.10 | 3.05 |
Nuclear Periphery Foci | -2.16 | -3.41 | -0.26 | 1.87 | 3.39 | 1.05 | -2.58 | 1.51 | 1.54 | 3.02 | 0.14 | -4.35 | 1.20 | 1.56 | 4.54 |
Nucleolus | -1.16 | -0.44 | 1.75 | 2.32 | 1.86 | -0.22 | 1.48 | 1.53 | 2.02 | 2.02 | -1.20 | 1.39 | 1.76 | 2.10 | 2.30 |
Nucleus | 1.51 | -1.97 | 3.88 | 1.76 | 2.93 | 0.70 | -2.71 | 3.36 | 2.74 | 3.85 | 0.85 | -3.37 | 5.19 | 2.69 | 4.78 |
Peroxisomes | -2.05 | 0.66 | 7.55 | 4.07 | 2.50 | 5.27 | 5.82 | 3.62 | 0.52 | -0.00 | 2.94 | 3.26 | 6.78 | 3.89 | 2.11 |
Vacuole | -4.11 | -5.00 | -1.66 | 1.62 | 4.49 | 0.60 | -5.88 | -0.97 | -1.13 | 5.58 | -3.10 | -7.69 | -1.94 | -0.84 | 7.14 |
Vacuole Periphery | -1.99 | 0.72 | -1.15 | 0.41 | -1.21 | 0.07 | 3.66 | 3.19 | 2.80 | -0.58 | -2.24 | 3.74 | -0.51 | 0.70 | -1.26 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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