Standard name
Description Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.64 0.58 0.58 0.57 0.52 0.48 0.46 0.43 0.36 0.49 0.4 0.4 0.48 0.49 0.4 0.45 0.45 0.42 0.28 0.29 0.22 0 0.09 0.12
Bud 0.26 0.29 0.25 0.25 0.34 0.22 0.22 0.23 0.22 0.19 0.24 0.19 0.12 0.19 0.15 0.2 0.19 0.23 0.34 0.37 0.37 0.49 0.46 0.48
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.21 0.21 0.23 0.28 0.33 0.22
Bud Site 0.29 0.11 0.11 0.15 0.12 0.16 0.1 0.08 0.11 0.1 0.06 0.09 0.14 0.09 0 0.06 0.09 0.09
Cell Periphery 0.14 0.15 0.15 0.23 0.06 0.11 0.13 0.09 0.16 0.11 0.1 0.15 0.18 0.25 0.38 0.12 0.13 0.08 0.11 0 0.09 0 0 0
Cytoplasm 0.07 0.15 0.15 0.16 0.2 0.19 0.19 0.23 0.14 0.11 0.11 0.12 0.19 0.14 0.16 0.16 0.17 0.16 0 0 0 0.09 0.06 0.12
Endoplasmic Reticulum 0 0 0.06 0.07 0 0.07 0.12 0.1 0.07 0.1 0.09 0 0.13 0.08 0.11 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.08 0.1 0 0 0 0 0 0
Mitochondria 0.19 0 0 0 0 0.11 0.08 0.11 0.29 0.23 0.57 0.29 0 0 0 0.11 0.08 0.11 0 0 0 0 0 0
Nucleus 0 0 0.05 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes 0.06 0 0 0 0 0 0.05 0 0.07 0.08 0.06 0.08 0 0.06 0.11 0 0.08 0.07 0 0 0 0 0 0
SpindlePole 0 0 0.09 0 0.08 0.05 0 0.07 0 0.05 0 0 0.08 0.14 0.1 0.1 0.12 0.13 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 149 104 82 109 108 110 129 127 105 138 121 146 89 112 105 163 140 127 78 68 42 2 12 18
Bud 62 51 36 47 71 51 61 68 63 53 73 69 23 44 39 74 57 68 96 87 71 24 68 74
Bud Neck 3 1 3 3 2 2 3 6 4 5 4 4 0 2 0 4 16 5 57 49 43 14 49 34
Bud Site 69 20 16 28 25 37 28 23 32 29 19 32 26 20 2 23 27 27
Cell Periphery 33 27 22 43 12 24 37 27 46 31 29 53 33 57 99 44 39 25 29 9 16 0 0 0
Cytoplasm 16 27 22 30 42 44 53 67 40 31 33 45 35 32 42 59 53 47 0 4 3 4 8 19
Endoplasmic Reticulum 9 8 9 14 9 15 34 28 19 29 27 16 25 18 28 8 13 8 3 2 1 0 1 0
Endosome 1 3 0 3 3 1 3 0 3 3 1 5 1 3 3 4 5 5 1 0 1 0 2 0
Golgi 2 2 1 1 0 0 1 3 0 1 0 1 1 1 4 48 25 30 3 1 0 0 0 0
Mitochondria 44 7 1 3 0 24 21 31 84 64 170 105 2 1 3 41 26 32 4 3 5 0 0 0
Nucleus 2 7 7 4 8 12 20 12 7 7 9 15 8 7 3 23 8 10 0 1 0 0 0 0
Nuclear Periphery 2 0 0 0 0 1 1 0 0 2 6 3 0 4 1 1 0 0 2 0 0 0 0 0
Nucleolus 4 3 0 2 7 6 8 12 9 9 11 24 0 4 0 11 6 14 0 0 0 1 0 0
Peroxisomes 15 4 3 7 2 8 13 11 19 22 17 30 8 13 29 13 25 21 0 0 0 0 1 0
SpindlePole 7 6 13 5 17 11 10 20 10 13 4 15 15 32 26 35 37 38 0 0 0 0 0 0
Vac/Vac Membrane 1 9 6 3 3 10 9 10 17 16 8 15 7 7 5 20 9 11 1 4 2 0 0 1
Unique Cell Count 234 178 142 191 206 228 278 294 291 284 299 364 186 229 260 366 308 299 282 238 191 51 149 156
Labelled Cell Count 419 279 221 302 309 356 431 445 458 453 532 578 273 357 389 571 486 468 282 238 191 51 149 156


Ambiguous, Bud, Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 10.6 9.5 9.1 9.2 8.3 8.1 8.1 7.3 7.3 6.6 7.1 9.9 10.0 10.2 12.6 12.2 11.6 10.6 9.7 10.2
Std Deviation (1e-4) 1.6 2.6 2.0 1.5 2.2 2.2 1.9 1.8 1.7 1.6 1.4 1.5 2.2 2.4 2.3 4.2 4.2 3.8 2.1 1.5 1.5
Intensity Change (Log2) -0.07 -0.06 -0.2 -0.24 -0.23 -0.38 -0.38 -0.53 -0.43 0.05 0.07 0.1 0.4 0.36 0.29 0.15 0.03 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.1 -1.0 -1.8 -2.2 -2.8 -4.3 -1.8 -3.4 -3.6 -1.8 -1.7 -3.3 -2.7 -2.4 -3.0
Bud -0.2 1.8 -0.7 -0.8 -0.5 -0.9 -1.6 -0.2 -1.6 -3.0 -1.4 -2.5 -1.3 -1.7 -0.6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 1.5 0
Bud Site 0.9 0.2 1.3 -0.4 -1.2 -0.1 -0.3 -1.8 -0.9 0.7 -0.8 -4.9 -1.9 -0.8 -0.7
Cell Periphery 1.6 -3.0 -1.4 -0.6 -2.0 0.1 -1.3 -1.8 -0.3 0.5 2.1 4.7 -1.0 -0.8 -2.3
Cytoplasm 0.1 1.2 0.9 0.9 1.8 -0.5 -1.3 -1.3 -0.9 0.8 -0.4 0.2 0.2 0.5 0.1
Endoplasmic Reticulum 0.4 -0.8 0.1 1.9 1.1 0.1 1.3 1.0 -0.9 2.1 0.5 1.5 0 -1.0 -1.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 4.3 3.1 3.6
Mitochondria 0 0 3.7 3.0 3.7 6.9 5.9 11.3 7.0 0 0 0 3.9 3.2 3.7
Nucleus 0 -0.5 0.1 0.9 -0.4 0 0 -1.0 -0.4 -0.3 -0.9 0 0.6 0 -0.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0
Peroxisomes 0 0 0 1.3 0 2.0 2.3 1.7 2.5 0 1.6 3.2 0 2.5 2.1
SpindlePole -2.6 -0.3 -1.6 -2.4 -0.9 -2.5 -1.9 -4.0 -2.2 -0.4 1.4 0.3 0.1 0.9 1.1
Vacuole 0 0 0.1 -0.5 -0.4 0.7 0.6 0 -0.1 -0.2 0 0 0.6 0 -0.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yap1802-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available