Standard name
Human Ortholog
Description 6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.91 0.97 0.95 0.93 0.93 0.79 0.72 0.76 0.64 0.68 0.53 0.55 1.0 0.98 1.0 – 0.94 0.95 0.76 0.85 0.58 0.9 0.84 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.12 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0.06 0.14 0.17 0.38 0.53 0.4 0.56 0.52 0.64 0.63 0 0 0 – 0 0 0.11 0.05 0.21 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 4 2 6 0 2 3
Bud 2 0 1 2 2 7 5 6 8 0 14 7 0 2 0 – 0 0 2 2 4 0 2 3
Bud Neck 0 3 0 1 0 2 1 5 2 3 1 1 0 0 0 – 3 2 1 1 1 0 0 0
Bud Site 0 0 0 0 0 1 2 0 1 1 3 2 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 3 3 0 2 2 0 0 3 0 0 2 2 1 1 – 4 5 1 1 0 0 1 1
Cytoplasm 230 273 231 174 174 216 185 198 154 148 157 136 183 208 270 – 197 153 201 236 145 92 150 155
Endoplasmic Reticulum 3 2 0 1 0 0 0 0 0 0 0 1 0 0 0 – 25 10 1 0 3 0 0 2
Endosome 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 – 2 3 4 2 6 1 0 1
Golgi 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 – 3 1 4 1 3 0 0 1
Mitochondria 41 9 14 27 32 103 136 105 134 113 187 154 2 1 2 – 4 4 27 15 52 1 8 3
Nucleus 0 0 2 0 1 1 1 0 1 0 1 1 0 1 0 – 0 0 0 0 0 0 1 0
Nuclear Periphery 1 1 0 0 0 0 0 0 0 2 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 2 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 – 0 1 1 0 1 0 0 1
SpindlePole 1 0 1 1 1 2 4 4 5 8 5 7 0 0 0 – 2 0 2 3 7 0 1 2
Vac/Vac Membrane 0 0 0 0 2 3 1 2 4 1 3 3 0 2 0 – 4 3 6 5 6 0 4 4
Unique Cell Count 252 281 243 187 187 273 256 262 239 217 294 246 183 212 271 209 161 264 277 250 103 179 186
Labelled Cell Count 280 291 255 206 214 338 337 320 312 276 373 315 187 216 273 244 182 264 277 250 103 179 186


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.9 3.8 3.3 3.5 2.9 2.5 3.0 2.7 2.6 2.7 2.5 3.9 4.0 4.1 4.6 5.5 5.6 3.8 4.4 5.0
Std Deviation (1e-4) 0.7 1.1 1.5 1.0 1.4 1.0 0.9 1.6 1.1 1.9 1.3 1.2 1.0 1.6 1.4 1.5 1.7 2.0 1.6 4.1 2.0
Intensity Change (Log2) – – – -0.23 -0.12 -0.4 -0.64 -0.35 -0.49 -0.55 -0.5 -0.65 0.04 0.06 0.09 0.27 0.51 0.55 -0.02 0.18 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7699 -0.3625 -0.3763 -0.5906 -0.9817 -0.3793 0.9832 0.8099 0.8755 1.4144 0.9215 0.8782 1.6135 1.4902 1.6967 2.6515 1.9264 1.8992
Actin 0.0157 0.0023 0.0031 0.0013 0.0015 0.0107 0.0586 0.0008 0.0076 0.0032 0.003 0.0096 0.0114 0.0006 0.0123 0.0016 0.037 0.0029
Bud 0.0005 0.0013 0.0005 0.0009 0.0001 0.0008 0.0028 0.0001 0.0015 0.0027 0.0017 0.0002 0.0032 0.0053 0.0026 0.0326 0.0003 0.0002
Bud Neck 0.0006 0.0003 0.004 0.0009 0.0001 0.0018 0.0032 0.0001 0.0005 0.0004 0.0023 0.0024 0.0006 0 0.0006 0.0002 0.0012 0.0001
Bud Periphery 0.0025 0.0007 0.0009 0.0016 0.0001 0.0027 0.0044 0.0002 0.0048 0.0027 0.002 0.0004 0.002 0.0059 0.002 0.0136 0.001 0.0001
Bud Site 0.0015 0.0103 0.0039 0.0012 0.0004 0.0003 0.0202 0.0063 0.0135 0.016 0.0489 0.0004 0.0066 0.0011 0.0057 0.005 0.0007 0
Cell Periphery 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0006 0.0002 0.0005 0.0002 0.0004 0 0.0011 0.0001 0.0004 0.0006 0.0001 0.0001
Cytoplasm 0.0536 0.0608 0.0797 0.0565 0.0811 0.0926 0.0224 0.0375 0.023 0.1178 0.0369 0.0646 0.0213 0.0206 0.021 0.0133 0.007 0.0399
Cytoplasmic Foci 0.0195 0.0061 0.0124 0.0054 0.0062 0.0136 0.0183 0.0068 0.0056 0.0171 0.0079 0.0104 0.0037 0.0032 0.0078 0.0052 0.0059 0.0021
Eisosomes 0.0006 0.0002 0.0004 0.0001 0.0002 0.0002 0.0009 0.0002 0.0005 0.0001 0.0001 0.0001 0.0008 0.0002 0.0003 0.0002 0.0005 0.0005
Endoplasmic Reticulum 0.0018 0.0007 0.0011 0.0009 0.0007 0.0006 0.0032 0.0007 0.0038 0.002 0.0006 0.0002 0.002 0.0003 0.0012 0.0014 0.0006 0.0002
Endosome 0.0039 0.001 0.0012 0.0007 0.0053 0.0016 0.0206 0.0032 0.006 0.0109 0.0223 0.0029 0.006 0.0009 0.0038 0.005 0.0083 0.0004
Golgi 0.0015 0.0001 0.0005 0.0001 0.0012 0.0011 0.0092 0.0005 0.0026 0.0022 0.007 0.0013 0.0022 0 0.0019 0.0004 0.0108 0.0003
Lipid Particles 0.0026 0.0003 0.0013 0.0003 0.0084 0.0015 0.0116 0.0003 0.004 0.0017 0.0119 0.0013 0.0033 0.0002 0.0019 0.002 0.0046 0.0028
Mitochondria 0.0018 0.0002 0.0013 0.0004 0.0006 0.0006 0.0057 0.0001 0.0137 0.0009 0.0034 0.0004 0.0006 0.0008 0.0024 0.0025 0.0069 0.0003
None 0.8746 0.9069 0.8722 0.9235 0.8844 0.8625 0.797 0.9412 0.9014 0.8115 0.8382 0.899 0.9149 0.9569 0.928 0.8757 0.9028 0.943
Nuclear Periphery 0.0028 0.0003 0.0009 0.0009 0.0012 0.0006 0.0059 0.0001 0.0018 0.0015 0.002 0.0002 0.0131 0.0006 0.0012 0.0195 0.0032 0.0001
Nucleolus 0.0021 0.0006 0.0008 0.0004 0.0004 0.0004 0.0005 0.0001 0.0003 0.0003 0.0007 0.0002 0.0003 0.0003 0.0005 0.0017 0.0002 0.0001
Nucleus 0.0013 0.0012 0.0021 0.0013 0.0005 0.001 0.0023 0.0003 0.0005 0.0011 0.0005 0.0004 0.0016 0.0012 0.0019 0.0079 0.0003 0.0001
Peroxisomes 0.0064 0.0002 0.0092 0.0003 0.0026 0.0008 0.0082 0.0002 0.0063 0.0018 0.0058 0.0042 0.0006 0.0002 0.0014 0.0011 0.0067 0.0055
Punctate Nuclear 0.0053 0.0061 0.0035 0.0025 0.0045 0.0063 0.0023 0.0004 0.0006 0.0028 0.0024 0.0017 0.0034 0.0008 0.0022 0.0072 0.0009 0.0012
Vacuole 0.0009 0.0003 0.0007 0.0006 0.0003 0.0003 0.0012 0.0004 0.0011 0.0028 0.0016 0.0001 0.0007 0.0007 0.0007 0.0022 0.0005 0.0002
Vacuole Periphery 0.0003 0 0.0001 0.0001 0.0002 0.0001 0.0008 0.0001 0.0005 0.0005 0.0004 0 0.0004 0.0002 0.0002 0.0009 0.0004 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.6112 5.3354 2.9693 3.0832 1.4509 1.4913 3.8774 2.5032 4.0387 3.1731
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2128 1587 542 1673 2162 2328 87 2040 4290 3915 629 3713

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 560.84 667.90 858.24 783.34 680.06 683.54 903.99 828.14 620.92 677.20 864.57 807.95
Standard Deviation 65.16 93.98 99.66 114.47 73.78 84.94 80.53 214.14 91.67 89.05 98.51 177.75
Intensity Change Log 2 — 0.252043 0.613792 0.482049 — 0.007364 0.410645 0.284213 — 0.123111 0.506015 0.376999

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000048 0.000391 0.000183 0.000578 0.000027 0.000626 0.000199 0.000881 0.000037 0.000531 0.000185 0.000744
Bud Neck 0.014770 0.041124 0.003554 0.008876 0.005739 0.059641 0.000809 0.012951 0.010219 0.052135 0.003175 0.011115
Bud Site 0.007811 0.014317 0.010051 0.025879 0.000839 0.024274 0.001127 0.045509 0.004297 0.020238 0.008816 0.036664
Cell Periphery 0.000826 0.000142 0.000157 0.000297 0.000089 0.000403 0.000283 0.000193 0.000455 0.000298 0.000174 0.000240
Cytoplasm 0.511218* 0.401785* 0.381263* 0.502290* 0.540303* 0.356074* 0.293338* 0.500730* 0.525876* 0.374604* 0.369101* 0.501433*
Cytoplasmic Foci 0.111606 0.264136* 0.023241 0.021899 0.118774 0.267672* 0.041898 0.014895 0.115218 0.266238* 0.025822 0.018051
Eisosomes 0.000330 0.000160 0.000058 0.000036 0.000079 0.000205 0.000148 0.000041 0.000203 0.000187 0.000070 0.000039
Endoplasmic Reticulum 0.001162 0.000785 0.002587 0.001168 0.000354 0.000540 0.003208 0.001196 0.000755 0.000640 0.002673 0.001184
Endosome 0.002910 0.028446 0.003309 0.006881 0.002309 0.029371 0.005986 0.005256 0.002607 0.028996 0.003680 0.005988
Golgi 0.001484 0.006153 0.000075 0.004948 0.000413 0.010284 0.000102 0.001531 0.000945 0.008610 0.000079 0.003071
Lipid Particles 0.009040 0.010044 0.002195 0.000821 0.003559 0.008035 0.005393 0.000882 0.006277 0.008849 0.002637 0.000855
Mitochondria 0.004181 0.013081 0.000318 0.004141 0.001340 0.024711 0.000230 0.004487 0.002749 0.019997 0.000306 0.004331
Mitotic Spindle 0.000253 0.001693 0.000821 0.052010 0.000084 0.011072 0.000053 0.061062 0.000168 0.007270 0.000714 0.056984
None 0.021894 0.006269 0.003901 0.007104 0.005261 0.002482 0.003092 0.004288 0.013512 0.004017 0.003789 0.005557
Nuclear Periphery 0.000636 0.000553 0.001381 0.001289 0.000376 0.000342 0.001417 0.001621 0.000505 0.000428 0.001386 0.001471
Nuclear Periphery Foci 0.000196 0.000379 0.002152 0.000564 0.000131 0.000171 0.001792 0.000566 0.000163 0.000255 0.002102 0.000565
Nucleolus 0.002359 0.003318 0.000573 0.000529 0.000746 0.001702 0.001129 0.000521 0.001546 0.002357 0.000650 0.000525
Nucleus 0.292267* 0.112084 0.492906* 0.269997* 0.301466* 0.102942 0.502251* 0.258222* 0.296903* 0.106648 0.494199* 0.263527*
Peroxisomes 0.001664 0.018167 0.000306 0.001508 0.001303 0.025228 0.000636 0.000901 0.001482 0.022366 0.000352 0.001175
Vacuole 0.014578 0.074222 0.070764 0.085825 0.016528 0.071766 0.136686 0.080394 0.015561 0.072762 0.079882 0.082841
Vacuole Periphery 0.000768 0.002750 0.000204 0.003361 0.000279 0.002459 0.000223 0.003871 0.000521 0.002577 0.000207 0.003641

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.53 -7.80 -3.31 0.14 -1.38 -4.78 -5.19 -2.85 0.61 -1.17 -6.26 -9.55 -4.00 0.69 -1.93
Bud Neck -9.99 8.13 5.10 13.35 -4.54 -21.53 9.56 -4.41 19.39 -11.02 -22.54 7.52 1.52 23.54 -6.65
Bud Site -4.09 -0.12 -3.48 -0.25 -2.23 -13.90 -3.74 -11.06 0.25 -10.61 -12.90 -1.37 -10.50 0.35 -4.93
Cell Periphery 7.39 7.11 6.22 -0.50 -0.07 -1.31 -6.00 -2.23 1.14 4.14 1.11 5.79 5.86 1.03 1.18
Cytoplasm 12.72 14.14 12.08 -0.56 -5.04 23.77 11.87 15.10 -7.50 -6.85 26.23 18.52 19.09 -6.12 -7.71
Cytoplasmic Foci -24.44 26.22 32.12 44.08 3.62 -25.37 13.29 32.17 52.52 4.82 -35.47 32.48 45.50 68.46 6.31
Eisosomes 8.49 13.33 15.08 15.77 5.29 -8.95 -5.41 6.88 11.68 7.54 1.41 10.88 16.00 16.39 7.81
Endoplasmic Reticulum 2.26 -8.12 -4.72 -10.29 5.80 -2.82 -7.05 -15.01 -12.19 4.67 1.35 -11.99 -11.13 -16.17 7.59
Endosome -15.13 -2.76 -1.31 14.19 0.57 -19.48 -4.86 -1.30 18.75 4.10 -24.58 -5.28 -1.74 23.40 2.80
Golgi -6.47 8.26 -0.60 4.22 -2.63 -12.37 6.77 -1.28 11.97 -3.54 -13.68 9.81 -0.73 11.52 -3.42
Lipid Particles -1.09 11.07 14.88 13.46 6.52 -7.74 -2.67 9.16 13.70 6.34 -4.99 8.71 17.26 19.18 8.52
Mitochondria -6.65 8.34 3.01 8.15 -4.09 -14.74 4.57 -1.81 13.59 -4.23 -15.72 9.32 1.09 15.82 -5.73
Mitotic Spindle -3.77 -2.33 -9.80 -9.33 -9.55 -7.41 -0.99 -11.53 -6.94 -8.34 -7.88 -2.46 -15.12 -11.01 -14.40
None 7.94 10.53 8.42 -0.02 -4.02 5.34 4.29 1.09 -5.37 -4.01 9.57 10.93 8.54 -2.63 -5.01
Nuclear Periphery 0.76 -11.31 -15.30 -13.90 -6.65 0.64 -6.24 -22.93 -22.32 -3.32 1.37 -15.46 -26.50 -25.60 -9.01
Nuclear Periphery Foci -4.13 -8.32 -5.85 -3.87 6.25 -2.67 -4.42 -8.50 -7.49 3.67 -4.31 -9.36 -9.41 -7.52 7.49
Nucleolus -1.39 4.68 4.59 4.63 -0.42 -6.73 -1.06 4.30 8.33 2.10 -2.63 4.65 5.15 7.15 0.96
Nucleus 28.75 -15.12 -5.70 -29.81 11.01 35.29* -5.71 -3.53 -34.32 4.77 45.51* -16.27 -6.53 -45.55 12.90
Peroxisomes -12.76 11.43 5.83 13.51 -2.57 -19.85 4.14 5.74 20.70 1.64 -23.48 11.67 8.44 24.61 -2.34
Vacuole -21.39 -13.17 -27.27 -14.30 -5.51 -21.92 -7.82 -29.38 -15.48 2.27 -30.43 -14.97 -40.10 -21.08 -4.02
Vacuole Periphery -7.93 5.96 -1.32 2.58 -2.59 -10.99 1.13 -2.64 1.93 -2.76 -13.07 5.87 -2.80 3.11 -3.81
Standard name
Human Ortholog
Description 6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (37%), nucleus (6%), mixed (48%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gnd2

Gnd2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gnd2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available