Standard name
Description 6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.91 0.97 0.95 0.93 0.93 0.79 0.72 0.76 0.64 0.68 0.53 0.55 1.0 0.98 1.0 – 0.94 0.95 0.76 0.85 0.58 0.9 0.84 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.12 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0.06 0.14 0.17 0.38 0.53 0.4 0.56 0.52 0.64 0.63 0 0 0 – 0 0 0.11 0.05 0.21 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 4 2 6 0 2 3
Bud 2 0 1 2 2 7 5 6 8 0 14 7 0 2 0 – 0 0 2 2 4 0 2 3
Bud Neck 0 3 0 1 0 2 1 5 2 3 1 1 0 0 0 – 3 2 1 1 1 0 0 0
Bud Site 0 0 0 0 0 1 2 0 1 1 3 2 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 3 3 0 2 2 0 0 3 0 0 2 2 1 1 – 4 5 1 1 0 0 1 1
Cytoplasm 230 273 231 174 174 216 185 198 154 148 157 136 183 208 270 – 197 153 201 236 145 92 150 155
Endoplasmic Reticulum 3 2 0 1 0 0 0 0 0 0 0 1 0 0 0 – 25 10 1 0 3 0 0 2
Endosome 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 – 2 3 4 2 6 1 0 1
Golgi 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 – 3 1 4 1 3 0 0 1
Mitochondria 41 9 14 27 32 103 136 105 134 113 187 154 2 1 2 – 4 4 27 15 52 1 8 3
Nucleus 0 0 2 0 1 1 1 0 1 0 1 1 0 1 0 – 0 0 0 0 0 0 1 0
Nuclear Periphery 1 1 0 0 0 0 0 0 0 2 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 2 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 – 0 1 1 0 1 0 0 1
SpindlePole 1 0 1 1 1 2 4 4 5 8 5 7 0 0 0 – 2 0 2 3 7 0 1 2
Vac/Vac Membrane 0 0 0 0 2 3 1 2 4 1 3 3 0 2 0 – 4 3 6 5 6 0 4 4
Unique Cell Count 252 281 243 187 187 273 256 262 239 217 294 246 183 212 271 209 161 264 277 250 103 179 186
Labelled Cell Count 280 291 255 206 214 338 337 320 312 276 373 315 187 216 273 244 182 264 277 250 103 179 186


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.9 3.8 3.3 3.5 2.9 2.5 3.0 2.7 2.6 2.7 2.5 3.9 4.0 4.1 4.6 5.5 5.6 3.8 4.4 5.0
Std Deviation (1e-4) 0.7 1.1 1.5 1.0 1.4 1.0 0.9 1.6 1.1 1.9 1.3 1.2 1.0 1.6 1.4 1.5 1.7 2.0 1.6 4.1 2.0
Intensity Change (Log2) – – – -0.23 -0.12 -0.4 -0.64 -0.35 -0.49 -0.55 -0.5 -0.65 0.04 0.06 0.09 0.27 0.51 0.55 -0.02 0.18 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7699 -0.3625 -0.3763 -0.5906 -0.9817 -0.3793 0.9832 0.8099 0.8755 1.4144 0.9215 0.8782 1.6135 1.4902 1.6967 2.6515 1.9264 1.8992
Actin 0.0157 0.0023 0.0031 0.0013 0.0015 0.0107 0.0586 0.0008 0.0076 0.0032 0.003 0.0096 0.0114 0.0006 0.0123 0.0016 0.037 0.0029
Bud 0.0005 0.0013 0.0005 0.0009 0.0001 0.0008 0.0028 0.0001 0.0015 0.0027 0.0017 0.0002 0.0032 0.0053 0.0026 0.0326 0.0003 0.0002
Bud Neck 0.0006 0.0003 0.004 0.0009 0.0001 0.0018 0.0032 0.0001 0.0005 0.0004 0.0023 0.0024 0.0006 0 0.0006 0.0002 0.0012 0.0001
Bud Periphery 0.0025 0.0007 0.0009 0.0016 0.0001 0.0027 0.0044 0.0002 0.0048 0.0027 0.002 0.0004 0.002 0.0059 0.002 0.0136 0.001 0.0001
Bud Site 0.0015 0.0103 0.0039 0.0012 0.0004 0.0003 0.0202 0.0063 0.0135 0.016 0.0489 0.0004 0.0066 0.0011 0.0057 0.005 0.0007 0
Cell Periphery 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0006 0.0002 0.0005 0.0002 0.0004 0 0.0011 0.0001 0.0004 0.0006 0.0001 0.0001
Cytoplasm 0.0536 0.0608 0.0797 0.0565 0.0811 0.0926 0.0224 0.0375 0.023 0.1178 0.0369 0.0646 0.0213 0.0206 0.021 0.0133 0.007 0.0399
Cytoplasmic Foci 0.0195 0.0061 0.0124 0.0054 0.0062 0.0136 0.0183 0.0068 0.0056 0.0171 0.0079 0.0104 0.0037 0.0032 0.0078 0.0052 0.0059 0.0021
Eisosomes 0.0006 0.0002 0.0004 0.0001 0.0002 0.0002 0.0009 0.0002 0.0005 0.0001 0.0001 0.0001 0.0008 0.0002 0.0003 0.0002 0.0005 0.0005
Endoplasmic Reticulum 0.0018 0.0007 0.0011 0.0009 0.0007 0.0006 0.0032 0.0007 0.0038 0.002 0.0006 0.0002 0.002 0.0003 0.0012 0.0014 0.0006 0.0002
Endosome 0.0039 0.001 0.0012 0.0007 0.0053 0.0016 0.0206 0.0032 0.006 0.0109 0.0223 0.0029 0.006 0.0009 0.0038 0.005 0.0083 0.0004
Golgi 0.0015 0.0001 0.0005 0.0001 0.0012 0.0011 0.0092 0.0005 0.0026 0.0022 0.007 0.0013 0.0022 0 0.0019 0.0004 0.0108 0.0003
Lipid Particles 0.0026 0.0003 0.0013 0.0003 0.0084 0.0015 0.0116 0.0003 0.004 0.0017 0.0119 0.0013 0.0033 0.0002 0.0019 0.002 0.0046 0.0028
Mitochondria 0.0018 0.0002 0.0013 0.0004 0.0006 0.0006 0.0057 0.0001 0.0137 0.0009 0.0034 0.0004 0.0006 0.0008 0.0024 0.0025 0.0069 0.0003
None 0.8746 0.9069 0.8722 0.9235 0.8844 0.8625 0.797 0.9412 0.9014 0.8115 0.8382 0.899 0.9149 0.9569 0.928 0.8757 0.9028 0.943
Nuclear Periphery 0.0028 0.0003 0.0009 0.0009 0.0012 0.0006 0.0059 0.0001 0.0018 0.0015 0.002 0.0002 0.0131 0.0006 0.0012 0.0195 0.0032 0.0001
Nucleolus 0.0021 0.0006 0.0008 0.0004 0.0004 0.0004 0.0005 0.0001 0.0003 0.0003 0.0007 0.0002 0.0003 0.0003 0.0005 0.0017 0.0002 0.0001
Nucleus 0.0013 0.0012 0.0021 0.0013 0.0005 0.001 0.0023 0.0003 0.0005 0.0011 0.0005 0.0004 0.0016 0.0012 0.0019 0.0079 0.0003 0.0001
Peroxisomes 0.0064 0.0002 0.0092 0.0003 0.0026 0.0008 0.0082 0.0002 0.0063 0.0018 0.0058 0.0042 0.0006 0.0002 0.0014 0.0011 0.0067 0.0055
Punctate Nuclear 0.0053 0.0061 0.0035 0.0025 0.0045 0.0063 0.0023 0.0004 0.0006 0.0028 0.0024 0.0017 0.0034 0.0008 0.0022 0.0072 0.0009 0.0012
Vacuole 0.0009 0.0003 0.0007 0.0006 0.0003 0.0003 0.0012 0.0004 0.0011 0.0028 0.0016 0.0001 0.0007 0.0007 0.0007 0.0022 0.0005 0.0002
Vacuole Periphery 0.0003 0 0.0001 0.0001 0.0002 0.0001 0.0008 0.0001 0.0005 0.0005 0.0004 0 0.0004 0.0002 0.0002 0.0009 0.0004 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.6112 5.3354 2.9693 3.0832 1.4509 1.4913 3.8774 2.5032 4.0387 3.1731
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –